PE anti-mouse CD3 100205 from BioLegend

Product Specs
ItemPE anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100205
NCBI Gene AliasesCtg-3, Ctg3, T3g
HostRat
ApplicationsFlow Cytometry (FC)
Conjugate/TagPE
ReactivityMouse
IsotypeIgG2b, Kappa
Quantity50 µg
Clone17A2
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) FGFR3 Alterations in Bladder Cancer Stimulate Serine Synthesis to Induce Immune-Inert Macrophages That Suppress T-cell Recruitment and ActivationCancer researchDecember 15, 2023Ouyang Y, Ou Z, Zhong W, Yang J, Fu S, Ouyang N, Chen J, Xu L, Wu D, Qian J, et al.Brilliant Violet 650 anti-CD8α (catalog no. 100741, BioLegend, RRID: AB_11124344); PE anti-CD3 (catalog no. 100206, BioLegend, RRID: AB_312663); PE/Cyanine7 anti-CD4 (catalog no. 100421, BioLegend, RRID: AB_312706);

(2) Transcription factor ETV4 promotes the development of hepatocellular carcinoma by driving hepatic TNF-α signalingCancer communications (London, England)December 1, 2023Qi D, Lu M, Xu P, Yao X, Chen Y, Gan L, Li Y, Cui Y, Tong X, Liu S, et al.FA‐11), anti‐F4/80 (123110, BM8), anti‐CD45 (103115, 30‐F11), anti‐CD11b (101212, M1/70), anti‐CD3 (100205, 17A2), anti‐CD4 (100515, RM4‐5), anti‐CD8 (100733, 53‐6.7), anti‐Ly6G (127617, 1A8) antibodies (BioLegend,

(3) IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8+ T Cells in the Tumor MicroenvironmentJournal of Clinical and Translational HepatologyNovember 28, 2023Lingyu Ma, Jiayu Jiang, Qin Si, Chong Chen, Zhaojun Duansoftware (version 10). The following antibodies (all from Biolegend, San Diego, CA, USA) were used: CD3 (100205), CD8 (100705), CD45 (103129), F4/80 (123125), CD206 (162505), I-A/I-E (MHCII) (107605), CD11b (101205),

(4) Analysis of ATG4C function in vivoAutophagyNovember 1, 2023Tamargo-Gómez I, Martínez-García GG, Suárez MF, Mayoral P, Bretones G, Astudillo A, Prieto-Lloret J, Sveen C, Fueyo A, Engedal N, et al.concentration according lot number. All the antibodies used in the study were from BioLegend: (PE-CD3, 100205; PE/Cy7-NK1.1, 108713; APC-CD19, 152409). Lc3B-Keima analyses For LC3B-Keima ratio analysis, cells

(5) C-type lectin receptor 2d forms homodimers and heterodimers with TLR2 to negatively regulate IRF5-mediated antifungal immunityNature CommunicationsOctober 23, 2023Fan Li, Hui Wang, Yan-Qi Li, Yebo Gu, Xin-Ming Jiawith FITC-conjugated anti-CD45 (Biolegend, 147710, dilution 1:200), PE-conjugated anti-CD3 (Biolegend, 100205, dilution 1:200), and BV421-conjugated anti-NK1.1 (Biolegend, 108741, dilution 1:200) antibodies. The

(6) Tracing immune cells around biomaterials with spatial anchors during large-scale wound regenerationNature CommunicationsSeptember 26, 2023Yang Yang, Chenyu Chu, Li Liu, Chenbing Wang, Chen Hu, Shengan Rung, Yi Man, Yili QueBioscience) and antibodies against surface markers CD45 (PE/Cyanine7, 147703, BioLegend), CD3 (PE, 100205, BioLegend), and F4/80 (FITC, 123107, BioLegend) at 1:400 dilution for 30 min at 4 °C in the dark (100

(7) Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometrySTAR ProtocolsSeptember 1, 2023Muddassar Hameed, Pallavi Rai, Melissa Makris, James Weger-Lucarelli1/100,BioLegend,AB_2227348 (Cat. No. 127614) PE anti-mouse CD3 antibody 1/100,BioLegend,AB_312663 (Cat. No. 100206) PerCP/Cyanine5.5 anti-mouse CD4 antibody 1/100,BioLegend,AB_2563023 (Cat. No. 116012) FITC anti-mouse CD8a

(8) METTL3 drives NAFLD-related hepatocellular carcinoma and is a therapeutic target for boosting immunotherapyCell Rep MedAugust 15, 2023Yasi Pan, Huarong Chen, Xiang Zhang, Weixin Liu, Yanqiang Ding, Dan Huang, Jianning Zhai, Wenchao Wei, Jun Wen, Danyu Chen, et al.IFN-γ(BV711) (4S.B3),Biolegend,Cat# 502540; RRID: AB_2563506 Anti-mouse CD3(PE) (17A2),Biolegend,Cat# 100206; RRID: AB_312663 Anti-human CD8(PE-Cy5) (SK1),Biolegend,Cat# 344770 Anti-mouse NK-1.1(PE-Cy7) (PK136),Biolegend,Cat#

(9) Synergistic efficacy of simultaneous anti-TGF-β/VEGF bispecific antibody and PD-1 blockade in cancer therapyJournal of hematology & oncologyAugust 12, 2023Niu M, Yi M, Wu Y, Lyu L, He Q, Yang R, Zeng L, Shi J, Zhang J, Zhou P, et al.then fluorescently stained with the following detection antibodies: α-CD45 (103132, BioLegend), α-CD3 (100206, BioLegend), α-CD8 (100706, BioLegend), α-Ki67 (151215, BioLegend), α-CD69 (104536, BioLegend), α-CD25

(10) Chronic pulmonary bacterial infection facilitates breast cancer lung metastasis by recruiting tumor-promoting MHCII(hi) neutrophilsSignal transduction and targeted therapyAugust 11, 2023Ma T, Tang Y, Wang T, Yang Y, Zhang Y, Wang R, Zhang Y, Li Y, Wu M, Tang M, et al.all purchased from Biolegend: CD45 (clone S18009, catalog no. 157214); CD3 (clone 17A2, catalog no. 100205); PD-1 (clone 29 F.1A12, catalog no. 135210); F4/80 (clone BM8, catalog no. 123109); CD11b (clone M1/70,

(11) Raman Spectroscopy Combined with Malaria Protein for Early Capture and Recognition of Broad-Spectrum Circulating Tumor CellsInternational Journal of Molecular SciencesJuly 28, 2023Xinning Liu, Yidan Zhang, Xunrong Li, Jian Xu, Chenyang Zhao, Jinbo Yanganti-IgG-AF488 secondary (A-10680) antibodies were purchased from Invitrogen (Waltham, MA, USA). Anti-CD3-PE (100205), anti-CD45-APC (368512), and anti-CD49b-PE (108908) antibodies were purchased from Biolegend (San Diego,

(12) High-fat-diet-associated intestinal microbiota exacerbates psoriasis-like inflammation by enhancing systemic γδ T cell IL-17 productionCell reportsJuly 25, 2023Sonomoto K, Song R, Eriksson D, Hahn AM, Meng X, Lyu P, Cao S, Liu N, Taudte RV, Wirtz S, et al.(clone 1D3),BD Biosciences,Cat#557655; RRID:AB_396770 Anti-mouse CD3-PE (clone 17A2),Biolegend,Cat#100205; RRID:AB_312662 TruStain FcX™ (anti-mouse CD16/32) Antibody,Biolegend,Cat#101320; RRID:AB_1574975 Anti-CD28

(13) Reversing the PAI-1-induced fibrotic immune exclusion of solid tumor by multivalent CXCR4 antagonistic nano-permeatorActa Pharmaceutica Sinica BJuly 1, 2023Jingwen Dong, Chenfei Zhu, Ying Huang, Quanhao Li, Jing Li, Zheng Wang, Yixin Wang, Zhanwei Zhou, Minjie Sunas follows: Brilliant Violet 421 anti-CD45 (cat#103133, Biolegend Co., Ltd.), PE anti-mouse CD3 (cat#100205, Biolegend Co., Ltd.), FITC anti-CD4 (cat#11603, Biolegend Co., Ltd.), APC anti-mouse CD8a (cat#100711,

(14) Large clones of pre-existing T cells drive early immunity against SARS-COV-2 and LCMV infectioniScienceJune 16, 2023Martina Milighetti, Yanchun Peng, Cedric Tan, Michal Mark, Gayathri Nageswaran, Suzanne Byrne, Tahel Ronel, Tom Peacock, Andreas Mayer, Aneesh Chandran, et al.AB_893330 Percp/cy5.5 CD8 mouse,Biolegend,Cat# 100733;RRID: AB_2075239 PE CD3 mouse,Biolegend,Cat# 100205;RRID: AB_312662 APC CD62L mouse,Biolegend,Cat# 104411;RRID: AB_313098 FITC CD44 mouse,Biolegend,Cat#

(15) Natural γδT17 cell development and functional acquisition is governed by the mTORC2-c-Maf-controlled mitochondrial fission pathwayiScienceMay 19, 2023Yunke Wang, Hui Qin, Yihua Cai, Xu Chen, Hong Li, Diego Elias Montoya-Durango, Chuanlin Ding, Xiaoling Hu, Julia H. Chariker, Harshini Sarojini, et al.17A2),Biolegend,Cat#100220 Anti-mouse CD3 APC/Cy7 (clone 17A2),Biolegend,Cat#100222 Anti-mouse CD3 PE (clone 17A2),Biolegend,Cat#100206 Anti-mouse γδTCR APC (clone GL3),Biolegend,Cat#118116 Anti-mouse γδTCR Percp/Cy5.5 (clone GL3),Biolegend,Cat#118118 Anti-mouse

(16) Effective-mononuclear cell (E-MNC) therapy alleviates salivary gland damage by suppressing lymphocyte infiltration in Sjögren-like diseaseFrontiers in Bioengineering and BiotechnologyApril 24, 2023Kayo Hasegawa, Jorge Luis Montenegro Raudales, Takashi I, Takako Yoshida, Ryo Honma, Mayumi Iwatake, Simon D. Tran, Makoto Seki, Izumi Asahina, Yoshinori SumitaAPC anti-mouse CD206 (MMR),"Biolegend, # 141707" "APC rat IgG2a, κ isotype Ctrl","Biolegend, # 400713" PE anti-mouse CD3,"Biolegend, # 100205" "PE Rat IgG2b, κ Isotype Ctrl","Biolegend, # 400607" APC-Cy7 anti-mouse CD4,"Biolegend, # 100413" "APC-Cy7

(17) A Beta Strain-Based Spike Glycoprotein Vaccine Candidate Induces Broad Neutralization and Protection against SARS-CoV-2 Variants of ConcernMicrobiology SpectrumApril 13, 2023Lei Cao, Jinyuan Guo, Hai Li, Hu Ren, Kang Xiao, Yan Zhang, Shuangli Zhu, Yang Song, Weijia Zhao, Dan Wu, et al.centrifuged and resuspended in 100 μL PBS. Then, 0.5 μL of 200-fold diluted PE anti-mouse CD3 (Biolegend, 100206), APC anti-mouse CD4 (Biolegend, 100412), and Alexa Fluor anti-mouse CD8 (Biolegend, 100100723) were

(18) Human adenovirus type 7 subunit vaccine induces dendritic cell maturation through the TLR4/NF-κB pathway is highly immunogenicFrontiers in Cellular and Infection MicrobiologyApril 12, 2023Yaru Li, Xia Yang, Renshuang Zhao, Zhiru Xiu, Shanzhi Li, Yue Li, Gaojie Song, Chenchen Ge, Jinbo Fang, Jicheng Han, et al.cat.105007), PE-CD40 (BioLegend, cat.124609), FITC-I-A/I-E (BioLegend, cat.), PE-CD3 (BioLegend, cat.100205), PerCP-CD4 (BioLegend, cat.100431), and FITC-CD8 (BioLegend, cat.100705) antibodies and the proportion

(19) An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarctionScience advancesApril 5, 2023Sun JY, Du LJ, Shi XR, Zhang YY, Liu Y, Wang YL, Chen BY, Liu T, Zhu H, Liu Y, et al.BioLegend), CD64-allophycocyanin (139305, BioLegend), Ly6G-phycoerythrin (127607, BioLegend), and CD3-PE (100205, BioLegend). Samples were analyzed using LSRFortessa (BD Biosciences). Enzyme-linked immunosorbent assay

(20) Induction of the activating transcription factor-4 in the intratumoral CD8+ T cells sustains their viability and anti-tumor activitiesCancer Immunology, ImmunotherapyApril 1, 2023Zhen Lu, Eun-Ah Bae, Ioannis I. Verginadis, Hongru Zhang, Christina Cho, Noreen McBrearty, Subin S. George, J. Alan Diehl, Constantinos Koumenis, Linda M. Bradley, et al.(Invitrogen, cat#A32732, 1:500), anti-CD45-APC/Cy7 (BioLegend, cat#157204), anti-CD3-PE (BioLegend, cat#100206), anti-CD8-AF700 (BioLegend, cat#100730), anti- CD69-BV421 (BioLgend, cat#104545, anti-PD-1-PE/Cy7 (BioLegend,

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12/1/2015CD3 PE Antibody Review Michaela RekowSplenocytes from latently infected mice are analyzed for various immune cells (T and B cells) and those are quantified at various time points of latent infection.

PE anti-mouse CD3 from BioLegend

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PE anti-mouse CD3 from BioLegend

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