Fig 1: Reprogramming of the Notch cellular status by posterior TFCs is for proper CpC specification. (A) Expression of the Notch activity reporter [E(spl)mß-CD2, green] and Dl protein (red) during the GSC niche formation. The Notch activity reporter and Dl protein are expressed in TFCs at ML3 stage. At LL3 stage, levels of the Notch reporter and the Dl protein are reduced in the most posterior TFC (blue asterisks). Dl appears in vesicles, indicating a Notch signal-sending state. At the prepupal stage, the Notch reporter is active in CpCs (yellow asterisks) and levels of the Notch reporter and Dl protein are reduced in the most posterior TFC (TC, blue asterisks). (B) The potential model of TFC reprograming from the Notch active to Dl-sending status.