Anti-Rad50 antibody [13B3/2C6] ab89 from Abcam

Product Specs
ItemAnti-Rad50 antibody [13B3/2C6]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab89
Antibody TypePrimary
Quantity100 UL
ClonalityMonoclonal
HostMouse, Nude mouse
IsotypeIgG1
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Clone13B3/2C6
Conjugate/TagUnconjugated
Format1 mg/ml of antibody purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE. Prepared in 10mM PBS (pH 7.4)
Concentration1 mg/ml
Gene Namerad50
ReactivityHuman
TargetRad50

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Targeting Mre11 overcomes platinum resistance and induces synthetic lethality in XRCC1 deficient epithelial ovarian cancersnpj Precision OncologyDecember 1, 2022Adel Alblihy et al.staining22, a set of slides were incubated with the primary anti-RAD50 mouse monoclonal antibody (clone ab89, Abcam), at a dilution of 1:400, for 1 h at room temperature. For LIG1 staining27, a set of slides were

(2) Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancersnpj Breast CancerDecember 1, 2021Adel Alblihy, Ahmed Shoqafi, Michael S. Toss, Mashael Algethami, Anna E. Harris, Jennie N. Jeyapalan, Tarek Abdel-Fatah, Juliette Servante, Stephen Y. T. Chan, Andrew Green, et al.were incubated with primary antibodies as follows: anti-MRE11(1:500, ab214, Abcam), anti-RAD50 (1:500, ab89, Abcam) and anti-NBS1 (1:500, N3162, Sigma) at 4 °C overnight. Samples then were washed and incubated

(3) Rad50 promotes ovarian cancer progression through NF-κB activationJournal of cellular and molecular medicineDecember 1, 2021Li Y, Wang S, Li P, Li Y, Liu Y, Fang H, Zhang X, Liu Z, Kong B.and immunoprecipitation were performed as described. 25 The primary antibodies included anti‐Rad50 (Abcam, USA, Cat# ab89; Santa Cruz, USA, Cat# sc‐74460), anti‐CARD9 (Protein Tech, USA, Cat# 10669–1‐AP; Santa Cruz, USA, Cat#

(4) ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibitionNucleic acids researchNovember 18, 2021Bakr A, Hey J, Sigismondo G, Liu CS, Sadik A, Goyal A, Cross A, Iyer RL, Müller P, Trauernicht M, et al.(Abcam, Cat#ab11169). Rabbit-anti-NBS1 (Novus Biologicals, Cat#NB100-143). Mouse-anti-RAD50 (Abcam, Cat#ab89). Rabbit-anti-RECQL (Abcam, Cat#ab151501). Mouse-anti-RPA32 (Santa Cruz, Cat#sc-53496). Mouse-anti-Flag-HRP

(5) Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stabilityThe Journal of biological chemistrySeptember 1, 2021Cao C, Han P, Liu L, Tang Y, Tian S, Zhang K, Shi L, Liu Z, Zhuo D, Ge W, et al.ABclonal; FLAG (F1804, for IP and WB) was purchased from Sigma; RAD51 (ab63801, for IF and WB) and RAD50 (ab89, for WB) were purchased from Abcam; BRCA1 (22362-1-AP, for IF and WB), KU70 (10723-1-AP, for WB), and

(6) 3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cellsJournal of molecular medicine (Berlin, Germany)March 1, 2021Dubich T, Dittrich A, Bousset K, Geffers R, Büsche G, Köster M, Hauser H, Schulz TF, Wirth D.to develop the signals. The primary antibodies used for Western blot analysis are as follows: RAD50 (ab89, Abcam), MRE11A (GTX70212, GeneTex) NBN (GTX103229, GeneTex), γH2AX S139 (R20244, NSJ Bioreagent), and

(7) Elevated double-strand break repair protein RAD50 predicts poor prognosis in hepatitis B virus-related hepatocellular carcinoma: A study based on Chinese high-risk cohortsJournal of CancerAugust 14, 2020Wangrui Liu, Wenhao Xu, Yuyan Chen, Liugen Gu, Xiaolei Sun, Yuanyuan Qu, Hailiang Zhang, Xiaojuan Liu, Haineng HuangImmunohistochemistry (IHC) Immunostaining of RAD50 was performed using a mouse monoclonal anti-RAD50 antibody (1:1000, #Ab89, Abcam, USA). Positive or negative staining of a specific protein on one formalin-fixed paraffin-embedded

(8) Rad50 mediates DNA demethylation to establish pluripotent reprogrammingExperimental & Molecular MedicineJuly 14, 2020Hanseul Park, Byounggook Cho, Jongpil Kimantibodies overnight at 4 °C. We used the following primary antibodies: rabbit anti-Rad50 (1:1000, ab89; Abcam), rabbit anti-Oct4 (1:1000, ab18976; Abcam), rabbit anti-methylcytosine dioxygenase (TET1) (1:1000,

(9) Vitamin D3 Ameliorates DNA Damage Caused by Developmental Exposure to Endocrine Disruptors in the Uterine Myometrial Stem Cells of Eker RatsCellsJune 12, 2020Elkafas H, Ali M, Elmorsy E, Kamel R, Thompson WE, Badary O, Al-Hendy A, Yang Q.1:1000 IF and F 1:200 RAD51 Abcam AB133534 Rabbit, monoclonal WB 1:5000 F 1:1000 RAD50 Abcam AB89 Mouse, monoclonal WB 1:5000 NBS1 Cell Signaling Technology 14959 Rabbit, monoclonal WB 1:1000 F

(10) A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancersPLoS biologyMarch 1, 2020Zhao K, Wang X, Xue X, Li L, Hu Y.follows: Antibodies against p-ATM (S1981) (#Ab81292), ATM (#Ab32420), NBS1 (#Ab32074), and RAD50 (#Ab89) were from Abcam. Antibodies against p-Chk2 (T68) (#2197), Mre11 (#4847), ATR (#13934), and p-ATR (S428)

(11) CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaksNucleic acids researchSeptember 26, 2019Hwang SY, Kang MA, Baik CJ, Lee Y, Hang NT, Kim BG, Han JS, Jeong JH, Park D, Myung K, et al.ChIP; #4895S from Cell Signaling Technology for IP), NBS1 (ab32074 from Abcam, 1:2000 for IB), RAD50 (ab89 from Abcam, 1:2000 for IB and IP), Tubulin (05-829 from Millipore, 1:3000 for IB), RAD51 (ab176458 from

(12) Hypovitaminosis D exacerbates the DNA damage load in human uterine fibroids, which is ameliorated by vitamin D3 treatmentActa pharmacologica SinicaJuly 1, 2019Ali M, Shahin SM, Sabri NA, Al-Hendy A, Yang Q.ab134826 Rabbit, polyclonal Anti-RAD50 Cell signaling technology, # 3427 Abcam, Rabbit, polyclonal ab89 Mouse, monoclonal WB, 1:1000 IF, 1:120 Anti-NBS1 Cell signaling technology, #14956 Rabbit, monoclonal WB,

(13) DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in gliomaEBioMedicineMarch 1, 2019Meng X, Duan C, Pang H, Chen Q, Han B, Zha C, Dinislam M, Wu P, Li Z, Zhao S, et al.anti-ATM (1:1000, Abcam, ab78), mouse anti-p53 (1:1000, Abcam, ab26), mouse anti-RAD50 (1:1000, Abcam, ab89), rabbit anti-γH2AX (1:1000, Abcam, ab2893), rabbit anti-C5 (1:500, ABclonal, A8104), rabbit anti-IL6

(14) Overexpression of the MRE11-RAD50-NBS1 (MRN) complex in rectal cancer correlates with poor response to neoadjuvant radiotherapy and prognosisBMC cancerSeptember 3, 2018Ho V, Chung L, Singh A, Lea V, Abubakar A, Lim SH, Ng W, Lee M, de Souza P, Shin JS, et al.incubated with mouse monoclonal primary antibodies including anti-RAD50 [13B3/2C6] (1:400 dilution, Abcam #ab89; Cambridge, UK), anti-MRE11 (1:600 dilution, Abcam #ab214) and anti-NBS1 (1:800 dilution, Novus Bioscience,

(15) MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End JoiningMolecular cellJuly 19, 2018Hung PJ, Johnson B, Chen BR, Byrum AK, Bredemeyer AL, Yewdell WT, Johnson TE, Lee BJ, Deivasigamani S, Hindi I, et al.anti-XRCC4,Santa Cruz,Cat#sc-8285 Mouse-anti-ATM,Sigma,Cat#A1106; clone MAT3-4G10/8 Mouse anti-RAD50,Abcam,Cat#ab89; clone 2C6 Rabbit anti-Mre11,Novus Biologicals,Cat#NB100-142 Rabbit anti-Nbs1,Abcam,Cat#ab23996 Rabbit

(16) Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repairCell researchJuly 1, 2018Li Z, Li Y, Tang M, Peng B, Lu X, Yang Q, Zhu Q, Hou T, Li M, Liu C, et al.ab17677), anti-H1.3 (Abcam, ab24174), anti-H3 (Abcam, ab1791), anti-H4 (Abcam, ab10158), anti-RAD50 (Abcam, ab89), anti-MRE11 (Abcam, ab12159), anti-phospho-H3 (S10; Abcam, ab5176), anti-ace-H3 (Active Motif, 39139),

(17) Unrepaired DNA damage in macrophages causes elevation of particulate matter- induced airway inflammatory responseAging (Albany NY)April 14, 2018Man Luo, Zhengqiang Bao, Feng Xu, Xiaohui Wang, Fei Li, Wen Li, Zhihua Chen, Songmin Ying, Huahao Shen(Santa Cruz Biotechnology, sc-9772), CD68( Servicebio, GB11067), Actin B (Santa Cruz Biotechnology, sc-47778), RAD50(Abcam, ab89), γH2AX (EMD Millipore, 05-636), RPA (Abcam, ab2175), pATM (EMD Millipore) and 53BP1 (EMD www.aging‐us.com 556 AGING GmbH,

(18) Early Postoperative Low Expression of RAD50 in Rectal Cancer Patients Associates with Disease-Free SurvivalCancers (Basel)November 30, 2017Vincent Ho et al.were then incubated with a monoclonal anti-RAD50 primary antibody [13B3/2C6] (1:400 dilution, Abcam #ab89; Cambridge, UK) for 60 min at room temperature. Following antigen retrieval, primary antibodies against

(19) Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated HerpesvirusJournal of virologyNovember 15, 2017Hollingworth R, Horniblow RD, Forrest C, Stewart GS, Grand RJ.(in-house), anti-K8.1 (in-house), anti-MRE11 (GTX70212; Genetex), anti-NBS1 (GTX70222; Genetex), anti-RAD50 (ab89; Abcam), anti-Ku80 (GTX70276; Genetex), anti-Ku70 (ab83501; Abcam), anti-BRCA1 (sc-6954; Santa Cruz),

(20) AUNIP/C1orf135 directs DNA double-strand breaks towards the homologous recombination repair pathwayNature communicationsOctober 17, 2017Lou J, Chen H, Han J, He H, Huen MSY, Feng XH, Liu T, Huang J.

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Anti-Rad50 antibody [13B3/2C6] from Abcam

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Anti-Rad50 antibody [13B3/2C6] from Abcam

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