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Anti-GAPDH antibody [6C5] - Loading Control
Anti-GAPDH antibody [6C5] - Loading Control from Abcam
(7)
Write a review
Citations:
(>1k)
Supplier Page
Supplier Page from
Abcam for
Anti-GAPDH antibody [6C5] - Loading Control
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-GAPDH antibody [6C5] - Loading Control
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab8245
Antibody Type
Primary
Clonality
Monoclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Host
Mouse, Nude mouse
Quantity
100 UG
Clone
6C5
Isotype
IgG1
Conjugate/Tag
Unconjugated
Format
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis
Concentration
2 mg/ml
Gene Name
GAPDH
Reactivity
Human, Mouse, Rat
Target
GAPDH
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (3967)
(1)
MALAT1 promotes FOXA1 degradation by competitively binding to miR-216a-5p and enhancing neuroendocrine differentiation in prostate cancer
Translational Oncology
January 1, 2024
Fanchang Zeng, Daoyuan Li, Xinli Kang, Qinghui Wu, Mi Song, Zhewen Ou, Zuobing Yang, Jing Yang, Liumei Luo
Abcam), SYP (
ab32127
,
Abcam
),
FOXA1
(
ab55178
,
Abcam
), ZFP91 (MA5–31,957) and the loading control GAPDH (
ab8245
,
Abcam
), followed by
incubation
with
horseradish
peroxidase-labeled goat anti-mouse IgG (
ab205719
,
1:20,000
).
(2)
GPR37-centered ceRNA network contributes to metastatic potential in lung adenocarcinoma: Evidence from high-throughput sequencing
Translational Oncology
January 1, 2024
Chuanhui Chen, Mengzhi Wan, Xiong Peng, Qing Zhang, Yu Liu
diluted rabbit primary antibodies against GPR37 (MAB44501,
1:1000
, Bio-Techne China, Shanghai) and GAPDH (
ab8245
,
1:2500
,
Abcam
). The next day, the
membrane
was further incubated with HRP-labeled goat anti-mouse IgG
(3)
MiR-18a-5p attenuates HER2-positive breast cancer development by regulating PI3K/AKT pathway
Cancer Biology & Therapy
December 31, 2023
Yongjun Liu, Hua Yang
(
ab8805
,
Abcam
), P-PI3K (ab278545, Abcam), PI3K (
ab32089
,
Abcam
, UK),
HER2
(
ab134182
,
Abcam
), and GAPDH (
ab8245
,
Abcam
) were incubated with the
membranes
after blocking at 37°C for an hour with 5% nonfat milk. The
(4)
Gallic acid improves the metformin effects on diabetic kidney disease in mice
Renal Failure
December 31, 2023
Yan Hong, Jidong Wang, Wenjuan Sun, Lai Zhang, Xuefang Xu, Kaiyue Zhang
EPR19549] to AMPK alpha 1 + AMPK alpha 2,
1:1000
), anti-
SIRT1
(#
ab110304
, 1.25 µg/mL), and anti-GAPDH (#
ab8245
,
1:1000
) from
Abcam
. Protein signals were detected using enhanced chemiluminescence (ECL). The DHE
(5)
LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway
Epigenetics
December 31, 2023
Fei Qi, Zhong-Dong Lv, Wen-Di Huang, Shan-Chen Wei, Xin-Min Liu, Wei-Dong Song
(
AP0094
,
1:500
,
ABclonal
), mTOR (
A2445
,
1:500
,
ABclonal
),
CDC27
(
A3333
,
1:1000
,
ABclonal
), and GAPDH (
ab8245
,
1:2000
,
Abcam
) at 4°C overnight, followed by
incubation
with secondary antibody (bs-0295 G-HRP,
1:1000
,
(6)
Pyrotinib and chrysin synergistically potentiate autophagy in HER2-positive breast cancer
Signal Transduct Target Ther
December 18, 2023
Xiaoxiao Liu, Xing Zhang, Zhiying Shao, Xiaorong Zhong, Xin Ding, Liang Wu, Jie Chen, Ping He, Yan Cheng, Kunrui Zhu,
et al.
(ab109012), p-eIEFA (ab157478), XBP-1 (ab37152), IRE (ab37073), G6PD (ab210702), ZBTB16 (ab104854) and GAPDH (
ab8245
), at 4 °C overnight. After washing in Tris-buffered saline with 0.05% Tween 20 (TBST), hybridized
membranes
(7)
Mesenchymal stem cells lose the senescent phenotype under 3D cultivation
Stem Cell Res Ther
December 18, 2023
O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. Neganova
Goat Anti-Mouse IgG (H + L)-HRP
) (
Abcam
, UK,
ab205719
). As a loading control we used GAPDH (
Abcam
, UK,
ab8245
). All experiments were performed as three biological repeats. Quantifications of
western blot
data were
(8)
Inhibitory effects of the nanoscale lysate derived from xenogenic dental pulp stem cells in lung cancer models
J Nanobiotechnology
December 18, 2023
Yan He, Ruohan Li, Wenting She, Yilong Ai, Kesheng Li, Tushar Kumeria, Ziran Jiang, Qing Shao, Chen Zou, Abdullkhaleg Ali Albashari,
et al.
anti-Bcl-2 (
ab182858
,
1:2000
), anti-Bax (
ab32503
,
1:1000
), anti-Bad (
ab32445
,
1:1000
), anti-GAPDH (
ab8245
,
1:500
) from
Abcam
(Cambridge, MA, USA).
Animal experiments
Male nude
mice
aged 6–8 weeks were obtained
(9)
Cyclophilin A facilitates influenza B virus replication by stabilizing viral proteins
iScience
December 15, 2023
Huizi Li, Wenhui Fan, Jie Min, Xiaoyuan Bai, Wenxian Yang, Heqiao Li, Yuna Zhao, Runshan Lin, Xiaojuan Jia, Wenjun Liu,
et al.
monoclonal anti-β-tubulin,
Abcam
,Cat#
ab6046
; RRID: AB_2210370 Mouse monoclonal anti-GAPDH,
Abcam
,Cat#
ab8245
; RRID: AB_2107448
Mouse
monoclonal
anti-Tubulin
(
clone
DM1A+DM1B),
Abcam
,Cat#
ab44928
; RRID: AB_2241150
Mouse
(10)
Effective protection of photoreceptors using an inflammation-responsive hydrogel to attenuate outer retinal degeneration
NPJ Regen Med
December 14, 2023
Hyerim Kim, Hyeonhee Roh, Sang-Heon Kim, Kangwon Lee, Maesoon Im, Seung Ja Oh
The
membranes
were incubated with primary antibodies including
1:5000
diluted anti-GAPDH antibody (#
ab8245
,
Abcam
, Cambridege, Cambridgeshire, UK),
1:1000
diluted anti-Phospho-STAT (#
9167
,
Cell Signaling
, Danvers,
(11)
Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3
Gut Microbes
December 11, 2023
Samira A. Hamed, Armaan Mohan, Saranya Navaneetha Krishnan, Arthur Wang, Marija Drikic, Nicole L. Prince, Ian A. Lewis, Jane Shearer, Åsa V. Keita, Johan D. Söderholm,
et al.
using
mouse
anti-
human
FFAR3 (
1:1,000
; #66811–1-1 g, Proteintech, Rosemount, IL) and GAPDH (
1:1,000
;
ab8245
,
Abcam
) antibodies, and a goat anti-mouse HRP-conjugated secondary antibody (
1:5,000
;
sc-2031
;
Santa Cruz
(12)
RAD54L2 counters TOP2-DNA adducts to promote genome stability
Sci Adv
December 6, 2023
Giuseppina D'Alessandro, David A. Morales-Juarez, Sean L. Richards, Karin C. Nitiss, Almudena Serrano-Benitez, Juanjuan Wang, John C. Thomas, Vipul Gupta, Andrea Voigt, Rimma Belotserkovskaya,
et al.
(
Sigma-Aldrich
,
SAB2108741
),
LIG4
(
ab193353
,
Abcam
), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (
ab8245
,
Abcam
).
Immunoprecipitation
For IP
, around 2 million RPE-1 cells were plated in 15-cm dishes (2 per
(13)
HO-1-Mediated Autophagic Restoration Protects Lens Epithelial Cells Against Oxidative Stress and Cellular Senescence
Invest Ophthalmol Vis Sci
December 5, 2023
Lijun Wang, Wei Lou, Yao Zhang, Ziang Chen, Yang Huang, Haiying Jin
(
ab208943
), anti-
TFEB
(
ab264421
), anti-
p21
(
ab188224
), anti-
p53
(
ab26
), anti–cyclin D1 (
ab16663
), anti-GAPDH (
ab8245
), and anti–Histone H3 (
ab1791
) primary antibodies were supplied from
Abcam
.
Lipofectamine 3000
(
L3000001
),
(14)
Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis
Redox Biol
December 2, 2023
Ufuk Ersoy, Ioannis Kanakis, Moussira Alameddine, Gibran Pedraza-Vazquez, Susan E. Ozanne, Mandy Jayne Peffers, Malcolm J. Jackson, Katarzyna Goljanek-Whysall, Aphrodite Vasilaki
(
ab73349
,
1:1000
), Anti-p62 (
ab91526
,
1:1000
), Anti-Citrate synthase (
ab129095
,
1:1000
), Anti-GAPDH (
ab8245
,
1:2000
) from
Abcam
(Cambridge, UK); Anti-AMPKα (#
2532
,
1:400
), Anti- Phospho-AMPKαThr172 (#
2535
,
1:400
),
(15)
Long non-coding RNA PCAT5 regulates the progression of Esophageal Squamous Cell Carcinoma via miR-4295/PHF20
Heliyon
December 1, 2023
Hui Liu, Hang Yin, Tao Yang, Jiacai Lin, Tingting Sun
severally incubated with primary antibodies against PHF20 (
ab67796
,
Abcam
, Cambridge, USA) and GAPDH (
ab8245
,
Abcam
). Secondary antibodies were added to culture for 1 h. Amount of protein was detected via chemiluminescence
(16)
HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice
Renal failure
December 1, 2023
Xing H, Li S, Fu Y, Wan X, Zhou A, Cao F, Sun Q, Hu N, Ma M, Li W,
et al
.
anti-LC3-I (ab62721;
1:1000
), anti-LC3-II (ab192890;
1:2000
), anti-p62 (ab109012;
1:10000
) and GAPDH (
ab8245
;
1:500
) at 4 °C overnight and subsequently with
horseradish
peroxidase-labeled IgG (
ab6721
;
1:1000
)
(17)
Icariin has the potential to induce the differentiation of bone marrow mesenchymal stem cells into brown fat cells via PDE5A inhibition
Heliyon
December 1, 2023
Yao Lu, Xiangfeng Xu, Sitong Liu, Zhaozheng Wang, Kai Jiang, Juan Gu, Li Fang, Rong Shen
ab129156
, 1/1000), PGC-1α (
Abcam
,
ab176328
, 1/1000), PDE5A (Abcam, ab259945, 1/1000) and GAPDH (
Abcam
,
ab8245
, 1/1000) at 4 °C overnight. GAPDH served as a loading control. Next morning,
membranes
were incubated
(18)
NRF2 is essential for adaptative browning of white adipocytes
Redox Biology
December 1, 2023
Marco Bauzá-Thorbrügge, Eduard Peris, Shabnam Zamani, Peter Micallef, Alexandra Paul, Stefano Bartesaghi, Anna Benrick, Ingrid Wernstedt Asterholm
MA1-112
;
Invitrogen
), anti-Histone H3 (
1:1000
dilution;
ab1791
;
Abcam
), anti-GAPDH (
1:2000
dilution;
ab8245
;
Abcam
), anti-betaTubulin (
1:1000
dilution;
2128
;
Cell signaling
), anti-Rabbit-HRP (
1:5000
; #
31461
;
(19)
Identification of a venetoclax-resistance prognostic signature base on 6-senescence genes and its clinical significance for acute myeloid leukemia
Frontiers in Oncology
November 30, 2023
Peng Ke, Jundan Xie, Ting Xu, Meiyu Chen, Yusha Guo, Ying Wang, Huiying Qiu, Depei Wu, Zhao Zeng, Suning Chen,
et al.
(35).
BAG3
Ab (
sc-136467
, United States) was purchased from
Santa Cruz Biotechnology
, and GAPDH Ab (
ab8245
, United States) was obtained from
Abcam
. G6PD Ab (AF6945, China) and secondary Abs, such as HRP goat
(20)
Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cells
Journal of Neuroinflammation
November 30, 2023
Zhaohui Ruan, Guosheng Cao, Yisong Qian, Longsheng Fu, Jinfang Hu, Tiantian Xu, Yaoqi Wu, Yanni Lv
temperature for 2 h, incubated with anti-
Lrg1
(
ab178698
,
Abcam
, Cambridge, England,
1: 1000
) and GAPDH (
ab8245
,
Abcam
, Cambridge, England,
1:1000
) primary antibodies in an ice
bath
for 24 h, and then incubated with
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Reviews
Write a Review
(1)
12/8/2023
Good GAPDH Antibody From Abcam for Western Blotting
Dr. David Sunnen
The specific goal of this western blot experiment was to evaluate the expression level of protein of interest relative to GAPDH protein expression in cell lysate samples. This antibody worked for ...
Read Review
(1)
8/7/2023
Best GAPDH Antibody From Abcam
Shivali Rawat
I use GAPDH antibody as loading control for total cellular protein fractions or soluble protein fractions regularly under various stress conditions.
(1)
9/13/2021
Great GAPDH Antibody
Jian Chen
GAPDH is a common loading control used for most western blots. This antibody has been working great for most of the cell lines we had worked at.
(1)
8/18/2016
Decent Loading Control Antibody
Shikha Saini
Using a loading control is extremely important for any conclusive experiment. Mostly, any gene which is vital for the cell or is abundantly present can be used as a loading control. GAPDH is one of ...
Read Review
(1)
2/1/2016
GAPDH Antibody for Western Blotting
Prudhvi Pusapati
I am interested in studying the role of various transcription factors during regenerative processes of the lung and intestine after chemical induced tissue injury. The level of transcription factors ...
Read Review
(1)
1/11/2016
Western Blotting GAPDH Antibody from Abcam
Ganesh Pusapati
GAPDH is considered like a house keeping gene and so its levels are used as a metric to determine the loading efficiency in gels (unless the experiment affects GAPDH levels. If yes, then an ...
Read Review
(1)
7/15/2015
Review of Anti-GAPDH antibody
Liyong Z.
To perform Western Blot analysis, the cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium ...
Read Review
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Anti-GAPDH antibody [6C5] - Loading Control from Abcam
Supplier Page
View Company Product Page
Pricing Info
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Company Info
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