Anti-Neurofilament heavy polypeptide antibody ab8135 from Abcam

Product Specs
ItemAnti-Neurofilament heavy polypeptide antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab8135
Antibody TypePrimary
ClonalityPolyclonal
HostRabbit
ApplicationsImmunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity50 UL
IsotypeIgG
Conjugate/TagUnconjugated
Gene NameNEFH
ReactivityMouse, Rat
TargetNeurofilament heavy polypeptide

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondiiCommunications BiologyNovember 27, 2023Thomas Grochow, Britta Beck, Zaida Rentería-Solís, Gereon Schares, Pavlo Maksimov, Christina Strube, Lisa Raqué, Johannes Kacza, Arwid Daugschies, Simone A. FietzAmsterdam, Netherlands, ab184267), neurofilament H (1:500, chicken, Abcam, Amsterdam, Netherlands, ab8135), SAG1 (1:1200, mouse, Bio-Rad Laboratories, Feldkirchen, Germany, 9070-2020), MAP2 (1:1000, chicken,

(2) Novel mutations in UL24 and gH rescue efficient infection of an HSV vector retargeted to TrkAMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Marco Marzulli, Bonnie L. Hall, Mingdi Zhang, William F. Goins, Justus B. Cohen, Joseph C. GloriosoSanta Cruz Biotechnology, sc-56986), TrkA (1:250, Thermo Fisher Scientific, 06–574), NF200 (1:250, Abcam, ab8135), TRPV1 (1:250, Santa Cruz Biotechnology, sc-286759), or TrkB (1:250, Santa Cruz Biotechnology, sc-12-G)

(3) Discovery of therapeutic targets for spinal cord injury based on molecular mechanisms of axon regeneration after conditioning lesionJournal of Translational MedicineJuly 28, 2023Xiaoxiong Wang, Wenxiang Li, Jianping Zhang, Jinze Li, Xianjin Zhang, Min Wang, Zhijian Wei, Shiqing Feng℃. Primary antibodies used were rabbit anti-neurofilament heavy polypeptide (NF200, 1:2000, Abcam, ab8135) and anti-serotonin (5-HT, 1:5000, Immunostar, 20080). The next day, the sections were incubated with

(4) Oscillating field stimulation promotes neurogenesis of neural stem cells through miR-124/Tal1 axis to repair spinal cord injury in ratsNeural regeneration researchApril 1, 2023Fang C, Sun J, Qian J, Shen CL.overnight. Longitudinal sections were incubated with primary antibody against neurofilament marker NF-H (ab8135, polyclonal antibody, rabbit anti-rat, 1:500, Abcam, RRID: AB_306298, Cambridge, MA, USA) at 4°C overnight.

(5) Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in miceCommunications biologyMarch 14, 2023Chou MY, Cao X, Hou KC, Tsai MH, Lee CY, Kuo MF, Wu VC, Huang HY, Akbarian S, Chang SK, et al.(1:1000, Sigma, G3893), mouse anti-NeuN (1:1000, Merck, MAB377), rabbit anti-Neurofilament (1:1000, Abcam, ab8135), mouse PSD95 (1:2000, Thermo Fisher, 7E3-1B8), rabbit anti-Synapsin I (1:1000, Merck, AB1543), and

(6) Organ- and function-specific anatomical organization of vagal fibers supports fascicular vagus nerve stimulationBrain StimulationMarch 1, 2023Naveen Jayaprakash, Weiguo Song, Viktor Toth, Avantika Vardhan, Todd Levy, Jacquelyn Tomaio, Khaled Qanud, Ibrahim Mughrabi, Yao-Chuan Chang, Moontahinaz Rob, et al.h of blocking step using one normal goat serum. Sections were incubated with anti-NF (1:500, Abcam AB8135), anti-MBP (1:500, Abcam AB7349) and either anti-ChAT (Millipore Sigma, AB144P) or anti-TH (Abcam, ab112

(7) Ang-(1-7)/MasR axis promotes functional recovery after spinal cord injury by regulating microglia/macrophage polarizationCell & bioscienceFebruary 4, 2023Gu G, Zhu B, Ren J, Song X, Fan B, Ding H, Shang J, Wu H, Li J, Wang H, et al.ab64693), anti-CD68(1:100, Abcam, ab283654), anti-GFAP (1:500, Abcam, ab53554), anti-NF200(1:500, abcam, ab8135). Flow cytometry The treated macrophages were digested into cell suspensions using 0.25% Trypsin–EDTA

(8) Phloretin enhances remyelination by stimulating oligodendrocyte precursor cell differentiationProceedings of the National Academy of Sciences of the United States of AmericaNovember 16, 2022Dierckx T, Vanherle S, Haidar M, Grajchen E, Mingneau F, Gervois P, Wolfs E, Bylemans D, Voet A, Nguyen T, et al.MAB386, Millipore; brain cryosections and in vitro OPC cultures), rabbit anti–neurofilament (1/1,000; Ab8135, Abcam), rat anti-MBP (1/250; MCA409S, Millipore; cerebellar brain slices), mouse anti-O4 (1/1,000;

(9) Insights into the Genetic Profile of Two Siblings Affected by Unverricht-Lundborg Disease Using Patient-Derived hiPSCsCellsNovember 4, 2022Valeria Lucchino, Luana Scaramuzzino, Stefania Scalise, Michela Lo Conte, Clara Zannino, Giorgia Lucia Benedetto, Umberto Aguglia, Edoardo Ferlazzo, Giovanni Cuda, Elvira Immacolata Parrottamouse monoclonal, #sc-32320, Santa Cruz, Dallas, TX, USA), anti-NEF-H (1:1000, rabbit polyclonal, #ab8135, Abcam, Cambridge, UK), and anti-MAP2 (1:1000, mouse monoclonal, #MA5-12826, Thermofisher Scientific,

(10) Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organizationCell reportsNovember 1, 2022Romano LEL, Aw WY, Hixson KM, Novoselova TV, Havener TM, Howell S, Taylor-Blake B, Hall CL, Xing L, Beri J, et al.RRID:AB_2138153 NES,Abcam,Ab22035; RRID:AB_446723 NEUN,Millipore,ABN90P; RRID:AB_2341095 NFH,Abcam,Ab8135; RRID:AB_306298 NUP98,Cell Signaling,#2598; RRID:AB_2267700 p-FAK (Tyr397),ThermoFisher,44–625G; RRID:AB_2533702 p-JNK

(11) Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasisMolecular metabolismNovember 1, 2022Frei IC, Weissenberger D, Ritz D, Heusermann W, Colombi M, Shimobayashi M, Hall MN.BML-CA1137-0100), GAP43-pS41 (1:200, R&D system, PPS006), Neurofilament heavy polypeptide (1:1000, Abcam, ab8135), Synaptophysin (1:200, Abcam, ab32127) and Adiponectin (1:500, Invitrogen, PA1-054) were used. After

(12) Metabolomics detects clinically silent neuroinflammatory lesions earlier than neurofilament-light chain in a focal multiple sclerosis animal modelJournal of NeuroinflammationOctober 9, 2022Tianrong Yeo, Halwan Bayuangga, Marcus Augusto-Oliveira, Megan Sealey, Timothy D. W. Claridge, Rachel Tanner, David Leppert, Jacqueline Palace, Jens Kuhle, Fay Probert, et al.Dako, Z0334) for astrocytes; anti-MBP (1:1000, Abcam, ab40390) for myelin, and anti-NfH (1:1000, Abcam, ab8135) for axons. Sections were then incubated with goat anti-rabbit biotinylated secondary antibody (Vectorlabs)

(13) Neuronal avalanche dynamics and functional connectivity elucidate information propagation in vitroFrontiers in Neural CircuitsSeptember 15, 2022Kristine Heiney, Ola Huse Ramstad, Vegard Fiskum, Axel Sandvig, Ioanna Sandvig, Stefano Nichele2 h blocking with 5% goat serum 0.3% Triton X in D-PBS−/−. Primary antibodies (neurofilament heavy (ab8135) at 1:1,000, GAD65 (ab26113) at 1:500, GABA-A (ab94585) at 1:100, GABA-B (ab55051) at 1:100, all primary

(14) Bioprinting of Human Neural Tissues Using a Sustainable Marine Tunicate-Derived Bioink for Translational Medicine ApplicationsInternational Journal of BioprintingAugust 24, 2022Soja Saghar Saghar Soman, Mano Govindraj, Noura Al Hashimi, Jiarui Zhou, Sanjairaj Vijayavenkataramanincubated with Rabbit Anti-Neurofilament heavy polypeptide (NEFH) antibody (1:50 in 1% BSA in PBS, ab8135, Abcam) at room temperature for 1 h. The scaffolds or constructs were washed with PBS for 3 times and

(15) Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophagesMolecular medicine (Cambridge, Mass.)June 29, 2022Yin G, Lin Y, Wang P, Zhou J, Lin H.(Agar Scientific). The primary antibodies used for IF were as follows: neurofilament (1:1000, Abcam, ab8135), SCG10 (1:500, Abcam, ab115513), IBA1 (1:100, Abcam, ab178847), and CD68 (1:100, Abcam, ab125212).

(16) Improving Control of Gene Therapy-Based Neurotrophin Delivery for Inner Ear ApplicationsFrontiers in Bioengineering and BiotechnologyJune 3, 2022Madeleine St. Peter, Douglas E. Brough, Anna Lawrence, Jennifer Nelson-Brantley, Peixin Huang, Jennifer Harre, Athanasia Warnecke, Hinrich StaeckerAntibody,Company,Product #,Concentration (mg/ml),Dilution/concentration (μg/ml),Source,Type Anti-NF,Abcam,ab8135,1.0,1:300 (3.33),Rabbit,Polyclonal Anti-TrkB,Alomone Labs,ANT-019-AG,1,1:50 (20),Rabbit,Polyclonal Anti-TrkC,Alomone Labs

(17) Recapitulation of endogenous 4R tau expression and formation of insoluble tau in directly reprogrammed human neuronsCell stem cellJune 2, 2022Capano LS, Sato C, Ficulle E, Yu A, Horie K, Kwon JS, Burbach KF, Barthélemy NR, Fox SG, Karch CM, et al.University",Cat# TOC1; RRID: AB_2832939 Rabbit polyclonal anti-Neurofilament Heavy Chain,Abcam,Cat# ab8135; RRID: AB_306298 Abberior StarRed goat anti-mouse,Abberior,Cat# STRED-1001-500UG Biological samples,, Fetal

(18) Excessive release of inorganic polyphosphate by ALS/FTD astrocytes causes non-cell-autonomous toxicity to motoneuronsNeuronMay 18, 2022Arredondo C, Cefaliello C, Dyrda A, Jury N, Martinez P, Díaz I, Amaro A, Tran H, Morales D, Pertusa M, et al.RRID:AB_94967 Rabbit anti-MAP2,Invitrogen,Cat# OSM00030W RRID:AB_10987424 Rabbit anti-SMI32,Abcam,Cat# ab8135 RRID:AB_306298 Mouse anti-SMI32,Abcam,Cat# ab187374 RRID:AB_2904559 Goat anti-ChAT,Sigma-Aldrich,Cat#

(19) Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal ActivityBiomedicinesMay 5, 2022Scalise S, Zannino C, Lucchino V, Lo Conte M, Scaramuzzino L, Cifelli P, D'Andrea T, Martinello K, Fucile S, Palma E, et al.polyclonal, 1:5000, PA1-10005, both from Thermo Fisher Scientific), anti-NEFH (rabbit polyclonal, 1:1000, ab8135, Abcam), anti-GAD1 (chicken polyclonal, 1:1000, AP31805PU-N, Origene, Rockville, MD, USA), anti-SST

(20) Low-Molecular Weight Protamine Overcomes Chondroitin Sulfate Inhibition of Neural RegenerationFrontiers in Cell and Developmental BiologyApril 25, 2022Natalia Kulesskaya, Ekaterina Mugantseva, Rimante Minkeviciene, Natalia Acosta, Ari Rouhiainen, Juha Kuja-Panula, Mikhail Kislin, Sami Piirainen, Mikhail Paveliev, Heikki Rauvalathe section interval 120 microns. The sections were stained with primary antibodies against NF200 (ab8135, Abcam, 1:500) in blocking buffer (1xTBS with 0.5% TX-100 and with 3%BSA) and AlexaFluor 594 (REF A11037,

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Anti-Neurofilament heavy polypeptide antibody from Abcam

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Anti-Neurofilament heavy polypeptide antibody from Abcam

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