Anti-Perilipin-1 antibody ab3526 from Abcam

Product Specs
ItemAnti-Perilipin-1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab3526
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
IsotypeIgG
ClonalityPolyclonal
Quantity100 UL
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NamePLIN1
ReactivityMouse
TargetPerilipin-1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Lepr‐Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1Advanced ScienceOctober 1, 2023Danting Zhang, Weimin Lin, Shuang Jiang, Peng Deng, Linfeng Liu, Qian Wang, Rui Sheng, Hui Sophie Shu, Lijun Wang, Weiguo Zou, et al.rabbit‐anti‐αSMA(1:400, ab184705, Abcam), rabbit‐anti‐Osterix(1:300, ab22552, Abcam), rabbit‐anti‐perilipin(1:800, ab3526, Abcam), mouse‐anti‐Aggrecan(1:200, sc‐166951, Santa cruz), rabbit‐anti‐PIEZO1(1:200, NBP1‐78446, Novus).

(2) Long-term NAD+ supplementation prevents the progression of age-related hearing loss in miceAging cellSeptember 1, 2023Okur MN, Sahbaz BD, Kimura R, Manor U, Patel J, Park JH, Andrade L, Puligilla C, Croteau DL, Bohr VA.the following antigens: CIDEC/Fsp27 (Ptglab, #12287‐1‐AP), PCK1 (Ptglab, #16754‐1‐AP), Plin1 (Abcam, Ab3526, 1:1000), Sirt1 (Santa Cruz, sc15404, 1:1000), GAPDH (Abclonal, AC027, 1:5000), PPARγ (Abcam, ab59256,

(3) Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitisExperimental & Molecular MedicineJuly 3, 2023Jung-Hwan Baek, Myung Sup Kim, Hye Ryeon Jung, Min-Seon Hwang, Chan-ho Lee, Dai Hoon Han, Yong-ho Lee, Eugene C. Yi, Seung-Soon Im, Ilseon Hwang, et al.cat. ab97533) at a 1:500 dilution for 1 h, anti-Perilipin1 rabbit polyclonal antibodies (Abcam, cat. ab3526) at a 1:1000 dilution for 1 h, anti-FABP4 rabbit polyclonal antibodies (Abcam, cat. ab13979) at a 1:2000

(4) Selection of Mechanical Fragmentation Methods Based on Enzyme-Free Preparation of Decellularized Adipose-Derived MatrixBioengineering (Basel)June 25, 2023Jiayi Feng, Su Fu, Jie Luantime point. Immunohistochemical staining was performed for the lipogenic marker Perilipin-1 (Abcam ab3526, Cambridge, UK, 1:1000) and CD31 (Abcam ab28364, Cambridge, UK, 1:4000) was used to locate vascular endothelial cells

(5) Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound HealingInternational Journal of NanomedicineJune 2, 2023Jia Dong, Bin Wu, Weidong Tian(1:1000, Abcam, ab187027), Histone H3 (1:1000, Invitrogen, PA5-16,183), perilipin A (1:1000, Abcam, ab3526), and actin (1:1000, Abcam, ab179467) at 4°C overnight. Horseradish peroxidase (HRP) conjugated secondary

(6) Wound infiltrating adipocytes are not myofibroblastsNature communicationsMay 25, 2023Kalgudde Gopal S, Dai R, Stefanska AM, Ansari M, Zhao J, Ramesh P, Bagnoli JW, Correa-Gallegos D, Lin Y, Christ S, et al.ab7778), Fibronectin 1 (1:250, Abcam ab23750), a-SMA (1:200, Abcam ab5694), Perilipin1 (1:200, Abcam, ab3526), CRAMP (1:200, Novus Biologicals, NB100-98689), FSP1 (1:200, Abcam, ab58597), PDGFRα (1:200, R&D Systems

(7) WNT7A suppresses adipogenesis of skeletal muscle mesenchymal stem cells and fatty infiltration through the alternative Wnt-Rho-YAP/TAZ signaling axisStem Cell ReportsApril 11, 2023Chengcheng Fu, Britney Chin-Young, GaYoung Park, Mariana Guzmán-Seda, Damien Laudier, Woojin M. Hanprimary and secondary antibodies were used for immunocytochemistry in this study: anti-PLIN (Abcam; ab3526; 1:200); anti- αSMA (Abcam; ab7817; 1:200); anti-YAP (Santa Cruz Biotechnology; sc101199; 1:200); anti-TAZ

(8) Human adipose tissue-derived small extracellular vesicles promote soft tissue repair through modulating M1-to-M2 polarization of macrophagesStem Cell Research & TherapyApril 7, 2023Jia Dong, Bin Wu, Weidong Tianincubated overnight at 4 °C with primary antibodies. Primary antibodies against perilipin A (1:200, Abcam, ab3526) and CD31(1:200, Santa Cruz, sc-376764) were used in this study for analyzing adipocytes and blood vessels.

(9) Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing heartsNature Cardiovascular ResearchMarch 23, 2023Linda Berg Berg Luecke, Matthew Waas, Jack Littrell, Melinda Wojtkiewicz, Chase Castro, Maria Burkovetskaya, Erin N. Schuette, Amanda Rae Buchberger, Jared M. Churko, Upendra Chalise, et al.antibodies (LSMEM2, cardiac troponin I (United States Biological, T8665–13F) and perilipin (Abcam, ab3526)) diluted in 10% normal goat serum at 4 °C. After three washes with DPBS, samples were incubated with

(10) Extracellular Lactic Acidosis of the Tumor Microenvironment Drives Adipocyte-to-Myofibroblast Transition Fueling the Generation of Cancer-Associated FibroblastsCellsMarch 20, 2023Elena Andreucci, Bianca Saveria Fioretto, Irene Rosa, Marco Matucci-Cerinic, Alessio Biagioni, Eloisa Romano, Lido Calorini, Mirko Manettidilution; catalog no. ab22554; Abcam), rabbit polyclonal anti-perilipin-1 (1:500 dilution; catalog no. ab3526; Abcam), mouse monoclonal anti-α-smooth muscle actin (α-SMA) (1:300 dilution; catalog no. ab7817; Abcam),

(11) SARS-CoV-2 infection induces persistent adipose tissue damage in aged golden Syrian hamstersCell Death & DiseaseFebruary 1, 2023Gemma Bogard, Johanna Barthelemy, Aline Hantute-Ghesquier, Valentin Sencio, Patricia Brito-Rodrigues, Karin Séron, Cyril Robil, Anne Flourens, Florence Pinet, Delphine Eberlé, et al.were used. For the detection of lipid droplets, rabbit polyclonal anti-perilipin-1 primary antibody (ab3526, Abcam) and HRP-conjugated goat anti-rabbit secondary antibody (Vector Laboratories) were used. Sample

(12) Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ miceCell death & diseaseNovember 19, 2022Farini A, Tripodi L, Villa C, Napolitano F, Strati F, Molinaro D, Facciotti F, Cassani B, Torrente Y.Novusbio); MYD88 (1:500, 23230-1-AP, Proteintech); AKT1/2/3 (1:500, ab126811, Abcam); Perilipin-1 (1:600, ab3526, Abcam); C3aR1 (1:100, PA5-109467 Invitrogen); pAkt (Ser473)(1:600, sc-81515, Santa Cruz Biotechnology);

(13) 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biologyInternational journal of obesity (2005)November 1, 2022Tews D, Brenner RE, Siebert R, Debatin KM, Fischer-Posovszky P, Wabitsch M.#536435, Minneapolis, UCA), rabbit anti-leptin (BioVendor #RD181001220), rabbit anti-perilipin A (Abcam #ab3526), rabbit anti-PPARG (Cell Signaling Technology #2443), rabbit anti-adiponectin (GeneTex #GTX112777),

(14) Dysregulated transforming growth factor-beta mediates early bone marrow dysfunction in diabetesCommunications BiologyOctober 28, 2022Jina J. Y. Kum, Christopher J. Howlett, Zia A. KhanScience, Hatfield, PA). Sections were incubated with rabbit anti-mouse perilipin-1 (PLIN1; Abcam; Cat# ab3526; 1:200 dilution) or insulin (INS; Thermo Fisher; Cat# 15848-1-AP; 1:1000 dilution) at room temperature

(15) The necroptosis-inducing pseudokinase mixed lineage kinase domain-like regulates the adipogenic differentiation of pre-adipocytesiScienceOctober 21, 2022Magusto J, Beaupère C, Afonso MB, Auclair M, Delaunay JL, Soret PA, Courtois G, Aït-Slimane T, Housset C, Jéru I, et al.3180 Anti-GAPDH,Cell Signaling Technology,Cat# 97166 Anti-MLKL,Abcam,Cat# ab172868 Anti-Perilipin-1,Abcam,Cat# ab3526 Anti-PPARγ,Protein Tech,Cat# 16643-1-AP Anti-RIPK3,Biorbyt,Cat# orb74415 Anti-Tubulin,Protein Tech,Cat#

(16) Targeting ABCC6 in Mesenchymal Stem Cells: Impairment of Mature Adipocyte Lipid HomeostasisInternational Journal of Molecular SciencesAugust 16, 2022Ricarda Plümers, Michel R. Osterhage, Christopher Lindenkamp, Cornelius Knabbe, Doris HendigSubsequently, cells were washed twice again before 2 h incubation with primary anti-PLIN1 antibody (ab3526; Abcam, Cambridge, UK) or anti-ATGL-antibody (ab207799; Abcam, Cambridge, UK) diluted 1:400 in 1% BSA

(17) Lipid droplet dynamics in healthy and pyometra-affected canine endometriumBMC veterinary researchJune 11, 2022Leitner N, Hlavaty J, Heider S, Ertl R, Gabriel C, Walter I.typ,Source,Catalogue nr.,Dilution anti-PLIN1 (anti-PLIN1 rabbit polyclonal antibody),IHC,primary,Abcam,ab3526,1:10000 anti-PLIN2 (ADRP (Perilipin 2) (AA5–27) mouse monoclonal antibody),IHC,primary,AntibodiesOnline,ABIN112185,1:1000 anti-PLIN3

(18) Morphological and Immunophenotypical Changes of Human Bone Marrow Adipocytes in Marrow Metastasis and MyelofibrosisFront Endocrinol (Lausanne)June 8, 2022Michele Dello Spedale Venti, Biagio Palmisano, Samantha Donsante, Giorgia Farinacci, Flavia Adotti, Ilenia Coletta, Marta Serafini, Alessandro Corsi, Mara Riminuccidiluted in phosphate buffer were used: polyclonal anti-human perilipin-1 (PLIN1) (Abcam, Cambridge, UK, ab3526, 1:500); polyclonal anti-human fatty acid binding protein 4 (FABP4) (Abcam, ab13979, 1:200); monoclonal

(19) Human Mesenchymal Stromal Cells Resolve Lipid Load in High Fat Diet-Induced Non-Alcoholic Steatohepatitis in Mice by Mitochondria DonationCellsJune 2, 2022Nickel S, Christ M, Schmidt S, Kosacka J, Kühne H, Roderfeld M, Longerich T, Tietze L, Bosse I, Hsu MJ, et al.Antigen,"Cat. No., Supplier",Dilution HepPar1 [OCH1E5],"MAB7927, Abnova, Taipeh, Taiwan",1:100 Perilipin 1,"ab3526, Abcam, Cambridge, England",1:600 E-cadherin,"610182, BD Biosciences, Heidelberg, Germany",1:200 β-catenin,"610154,

(20) Skin Fibrosis and Recovery Is Dependent on Wnt Activation via DPP4Journal of Investigative DermatologyJune 1, 2022Anna R. Jussila, Brian Zhang, Elizabeth Caves, Sakin Kirti, Miarasa Steele, Emily Hamburg-Shields, John Lydon, Yan Ying, Robert Lafyatis, Sanjay Rajagopalan, et al.immunohistochemical staining with β-Catenin (BD Biosciences, 1:250), myc-tag (Abcam ab9106, 1:500), Perilipin 1 (Abcam ab3526, 1:500), and CD26/DPP4 (R&D: AF954, 1:150) antibodies, western blot CD26/DPP4 (R&D: AF954, 0.25 μg/mL),

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Anti-Perilipin-1 antibody from Abcam

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Anti-Perilipin-1 antibody from Abcam

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