Acetyl-alpha-Tubulin (Lys40) (D20G3) XP ® Rabbit mAb 5335S from Cell Signaling Technology

Product Specs
ItemAcetyl-alpha-Tubulin (Lys40) (D20G3) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5335S
Quantity100 µl
ApplicationsW, IP, IF-F, IF-IC, FC-FP
ReactivityH, M, R, Mk, Z
Targettubulin alpha (Lys40) acetylate
Modificationstubulin alpha (Lys40) acetylate
HostRabbit
NCBI Gene AliasesK-ALPHA-1
ClonalityMonoclonal
NCBI Full Gene Nametubulin alpha 1b
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD20G3
Molecular Weight52

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cellsNPJ MicrogravityDecember 16, 2023Nancy Garbacki, Jérôme Willems, Thibaut Neutelings, Charles Lambert, Christophe Deroanne, Astrid Adrian, Markus Franz, Matthias Maurer, Philippe De Gieter, Betty Nusgens, et al.mouse anti-α-tubulin (1:1000, Sigma, #T6199), rabbit anti-acetyl-α-tubulin (1:500, Cell Signaling #5335P), mouse anti-LAMP2 (5 µg ml−1, DSHB #H4B4), rabbit anti-pFAK (1:500, Invitrogen #700255). The following

(2) Novel variants in TUBB8 gene cause multiple phenotypic abnormalities in human oocytes and early embryosJournal of Ovarian ResearchNovember 25, 2023Tingwenyi Hu, Chong Li, Sen Qiao, Weiwei Liu, Wei Han, Wei Li, Rong Shi, Xia Xue, Juanzi Shi, Guoning Huang, et al.Myc epitope (1:500 dilution, AM926-1, Beyotime) to visualize transgene and α-TUBULIN (1:800 dilution, 5335s, CST) to visualize endogenous microtubule network. The next day, cells were incubated with Goat anti

(3) Circular RNA from Tyrosylprotein Sulfotransferase 2 Gene Inhibits Cisplatin Sensitivity in Head and Neck Squamous Cell Carcinoma by Sponging miR-770-5p and Interacting with NucleolinCancers (Basel)November 9, 2023Tianqing Wang, Chuan Xin, Shiyu Zhang, Xin Tian, Yuting Hu, Ying Wang, Jiongke Wang, Ning Ji, Xin Zeng, Jing Lidilution rate 1:1000), histone H2A. X (9718 S, Cell Signaling Technology, dilution rate 1:1000), tubulin (5335 S, Cell Signaling Technology, dilution rate 1:1000), nucleolin (ab129200, Abcam, dilution rate 1:10,000),

(4) The HDAC6-RNF168 axis regulates H2A/H2A.X ubiquitination to enable double-strand break repairNucleic acids researchSeptember 22, 2023Qiu L, Xu W, Lu X, Chen F, Chen Y, Tian Y, Zhu Q, Liu X, Wang Y, Pei XH, et al.(Abcam, ab92450), anti-PARP1 (proteintech, 13371-1-AP), anti-Ac-α-tubulin (Cell Signaling Technology, 5335S), anti-HDAC1 (Santa Cruz, sc-6298), anti-HDAC2 (Cell Signaling, 2545), anti-H2A.X (Cell Signaling, 2595),

(5) Endogenous expression of inactive lysine deacetylases reveals deacetylation-dependent cellular mechanismsPLOS ONESeptember 18, 2023Tasha B. Toro, Elena V. Skripnikova, Kiara E. Bornes, Kun Zhang, Terry J. Wattβ-Tubulin (Developmental Hybridoma Studies Bank E7), α-Tubulin-acetyl-K40 (Cell Signaling Technologies 5335), Histone3 (Cell Signaling Technologies 4499), Histone H3-acetyl-K9 (Cell Signaling Technologies 9649),

(6) Nosip is a potential therapeutic target in hepatocellular carcinoma cellsiScienceAugust 18, 2023Junjie Gao, Dandan Yang, Zheng Huang, Xueshan Pan, Ruoxue Cao, Chaoqun Lian, Jia Ma, Yuyun Li, Zhiwei Wang, Jun XiaRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-NOSIP,Proteintech,Cat# 27979-1-AP;RRID: AB_2881027 Anti-Tubulin,CST,Cat#5335; HRP- conjugated β-actin,Proteintech,Cat#HRP-66009; RRID:AB_2883836 ,, "Chemicals, peptides, and

(7) Ciliary ARL13B prevents obesity in micebioRxiv : the preprint server for biologyAugust 4, 2023Terry TT, Gigante ED, Alexandre CM, Brewer KM, Engle SE, Yue X, Berbari NF, Vaisse C, Caspary T.anti-ARL13B (1:1000, NeuroMab, N295B/66); rabbit anti-acetylated α-tubulin (1:1000,Cell Signaling, 5335); mouse anti-acetylated α-tubulin (1:2500, Sigma, T7451); chicken anti-ACIII (1:1000, Encor, CPCA-ACIII);

(8) A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lungExperimental & molecular medicineAugust 1, 2023Lee W, Lee S, Yoon JK, Lee D, Kim Y, Han YB, Kim R, Moon S, Park YJ, Park K, et al.anti-SCGB1A1 (1:200, R&D Systems, MAB4218), rabbit anti-acetyl-α-tubulin (1:200, Cell Signaling Technology, 5335), mouse anti-SARS-CoV NP (1:150, Sino Biological, 40143-MM05), mouse anti-influenza A NP (1:100, Meridian,

(9) Influenza A–induced cystic fibrosis transmembrane conductance regulator dysfunction increases susceptibility to Streptococcus pneumoniaeJCI InsightJuly 24, 2023Erin Y. Earnhardt, Jennifer L. Tipper, Adonis D’Mello, Ming-Yuan Jian, Elijah S. Conway, James A. Mobley, Carlos J. Orihuela, Hervé Tettelin, Kevin S. Harrod30 minutes. HBECs were stained with primary antibodies against α-tubulin (rabbit monoclonal antibody 5335S, Cell Signaling Technology), IAV (mouse monoclonal antibody MAB8251, EDM Millipore), and F-actin conjugated

(10) Design, Synthesis, and Biological Evaluation of Novel Quinazolin-4(3H)-one-Based Histone Deacetylase 6 (HDAC6) Inhibitors for Anticancer ActivityInternational Journal of Molecular SciencesJuly 3, 2023Yogesh Mahadu Khetmalis, Ashna Fathima, Markus Schweipert, Cécile Debarnot, Naga Venkata Madhusudhan Rao Bandaru, Sankaranarayanan Murugesan, Trinath Jamma, Franz-Josef Meyer-Almes, Kondapalli Venkata Gowri Chandra Sekharantibodies of acetyl-H3 (CST (Danvers, MA, USA), 9677S), total H3 (CST, 9715S), acetyl-α tubulin (CST, 5335T), total α-tubulin (CST, 2125S), cleaved PARP (CST, 5625T), and β-actin (Sigma Aldrich (Burlington, MA,

(11) A COMPARATIVE ANALYSIS TO DETERMINE THE OPTIMUM HISTONE DEACETYLASE INHIBITORS AND ADMINISTRATION ROUTE FOR IMPROVING SURVIVAL AND ORGAN INJURY IN RATS AFTER HEMORRHAGIC SHOCKShockJuly 1, 2023Kunwei Niu, Long Yang, Wenjie Song, Zhengcai Liu, Juzheng Yuan, Hong Zhang, Wenjie Zhang, Jianlin Wang, Kaishan Taoanti-acetylated histone H4 (AC-H4, CST, #8647, 1:1,000), rabbit antiacetylated α-tubulin (AC-tubulin, CST, #5335, 1:1,000), rabbit antihistone H3 (H3, CST, #4499, 1:2,000), rabbit anti-histone H4 (H4, CST, #13919,

(12) Mechanisms of cilia regeneration in Xenopus multiciliated epithelium in vivobioRxivJune 18, 2023Venkatramanan G. Rao, Vignesharavind Subramanianbalachandar, Magdalena M. Magaj, Stefanie Redemann, Saurabh S. Kulkarnifix, 1:1000" Rabbit mAb Acetyl-α-Tubulin (Lys40) (D20G3),Ciliary axoneme,Cell Signalling technology (#5335),"Methanol Fix, 1: 500" Rabbit mAb Acetyl-α-Tubulin (Lys40) (D20G3) (Alexa Fluor® 647 Conjugate),Ciliary

(13) LRRC6 regulates biogenesis of motile cilia by aiding FOXJ1 translocation into the nucleusCell communication and signaling : CCSJune 16, 2023Kim DY, Sub YJ, Kim HY, Cho KJ, Choi WI, Choi YJ, Lee MG, Hildebrandt F, Gee HY.(NBP1-93,613; Novus Biologicals, Littleton, CO, USA), acetylated α-tubulin (T7451 from Sigma-Aldrich and 5335S from Cell Signaling Technology, Danvers, MA, USA), FLAG (#8146; Cell Signaling Technology, Danvers,

(14) The macrocyclic lactone oxacyclododecindione reduces fibrosis progressionFrontiers in PharmacologyJune 13, 2023Sabrina Saurin, Myriam Meineck, Markus Rohr, Wilfried Roth, Till Opatz, Gerhard Erkel, Andrea Pautz, Julia Weinmann-MenkeE-cadherin (24E10, 3195), Snail (C15D3, 3879), α-tubulin (11H10; 2125), and acetyl-α-tubulin (D20G3, Lys40; 5335) (all Cell Signaling Technology, Danvers, United States) antibodies. Antibodies against β-actin (13E5;

(15) linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cellseLifeMay 16, 2023Valerie A Tornini, Liyun Miao, Ho-Joon Lee, Timothy Gerson, Sarah E Dube, Valeria Schmidt, François Kroll, Yin Tang, Katherine Du, Manik Kuchroo, et al.(rabbit); anti-GFP (mouse, A11120, Thermo Fisher Scientific, 1:500); acetylated α-tubulin (rabbit, 5335T, Cell Signaling Technology, 1:500). Alexa Fluor 488, 546 or 568 secondary antibodies against rabbit

(16) Omicron Spike confers enhanced infectivity and interferon resistance to SARS-CoV-2 in human nasal tissuebioRxivMay 11, 2023Guoli Shi, Tiansheng Li, Kin Kui Lai, Jonathan W Yewdell, Alex A Comptonpermeabilized with 0.1% Triton-X, and stained with anti-Acetyl-a-Tubulin (Lys40) (D20G3) (Cell Signaling; 5335) and DAPI to assess pseudostratification and the presence of ciliated cells. Images were obtained using

(17) Hydrogen Peroxide Induces α-Tubulin Detyrosination and Acetylation and Impacts Breast Cancer Metastatic PhenotypesCellsApril 27, 2023Megan B. Stemberger, Julia A. Ju, Keyata N. Thompson, Trevor J. Mathias, Alexandra E. Jerrett, Katarina T. Chang, Eleanor C. Ory, David A. Annis, Makenzy L. Mull, Darin E. Gilchrist, et al.Cat. #2144S), anti-acetylated-α-tubulin (1:1000, Cell Signaling Technologies, Danvers, MA, USA, Cat. #5335S), anti-detyrosinated-α-tubulin (1:10,000, ReVmAb Biosciences, South San Francisco, CA, USA, Cat. #31-1335-00),

(18) PIMT is a novel and potent suppressor of endothelial activationeLifeApril 18, 2023Zhang C, Guo ZF, Liu W, Kazama K, Hu L, Sun X, Wang L, Lee H, Lu L, Yang XF, et al.anti-phospho-p44/42 MAPK (Erk1/2; #4370), anti-p44/42 MAPK (Erk1/2; #4695), and anti-acetyl-α-Tubulin (Lys40; #5335) antibodies were purchased from Cell Signaling Technology. Anti-Lamin A/C (sc-20681), anti-ICAM-1 (sc-7891),

(19) RSV-induced expanded ciliated cells contribute to bronchial wall thickeningVirus ResearchApril 2, 2023Sattya N. Talukdar, Jaspreet Osan, Ken Ryan, Bryon Grove, Danielle Perley, Bony D. Kumar, Shirley Yang, Sydney Dallman, Lauren Hollingsworth, Kristina L. Bailey, et al.polyclonal (1:100) (48-8400, Thermo Fisher Scientific), anti-acetyl-α-tubulin rabbit monoclonal (1:500) (5335, Cell Signaling Technologies), anti-MUC5AC mouse polyclonal (1:100) (H00004586-A01, Abnova), anti-Respiratory Syncytial Virus

(20) Mucociliary Wnt signaling promotes cilia biogenesis and beatingNature communicationsMarch 6, 2023Seidl C, Da Silva F, Zhang K, Wohlgemuth K, Omran H, Niehrs C.Proteintech, Cat#11770-1-AP; Rabbit monoclonal anti-Acetyl-α-Tubulin (Lys40), Cell Signaling Technology, Cat#5335T; Mouse monoclonal anti-TSG101 (4A10), Abcam, Cat#ab83). Secondary antibodies (Donkey polyclonal Anti-Mouse

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4/14/2016Acetylated Alpha Tubulin Claire StewartAcetylated tubulin has been implicated in ER localization and ER-mitochondrial interactions and many other functions associated with cellular homeostasis. This antibody was chosen to assess the amount... Read Review

Acetyl-alpha-Tubulin (Lys40) (D20G3) XP ® Rabbit mAb

Acetyl-alpha-Tubulin (Lys40) (D20G3) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (256)

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Acetyl-alpha-Tubulin (Lys40) (D20G3) XP ® Rabbit mAb from Cell Signaling Technology

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