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Antibodies
Antibodies
Anti-Argonaute-2 antibody
Anti-Argonaute-2 antibody from Abcam
Be the first to write a review!
Citations:
(31)
Supplier Page
Supplier Page from
Abcam for
Anti-Argonaute-2 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Argonaute-2 antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab5072
Antibody Type
Primary
Quantity
100 UG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Applications
Western blot
Conjugate/Tag
Unconjugated
Concentration
0.5 mg/ml
Research Area
Epigenetics
Gene Name
AGO2
Reactivity
Drosophila melanogaster
Target
Argonaute-2
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (31)
(1)
HNRNPA2B1-mediated m(6)A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis
Journal of translational medicine
June 12, 2023
Li K, Gong Q, Xiang XD, Guo G, Liu J, Zhao L, Li J, Chen N, Li H, Zhang LJ,
et al
.
manufacturer’s instructions. Antibodies against Ago2 and IgG for RIP
assays
were purchased from
Abcam
(
ab5072
, Cambridge, MA, USA). Anti-m6A antibody (
A-1801
–020,
Epigentek
) were used for MeRIP
assay
.
Luciferase reporter assay
(2)
Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs
Experimental & Molecular Medicine
November 16, 2022
Fei Zhang, Wuxun Peng, Tao Wang, Jian Zhang, Wentao Dong, Chuan Wang, Zhihong Xie, Hong Luo, Gang Liu
Abcam
), rabbit anti-PARP1/Cleaved
PARP1
(
1:10000
;
ab191217
;
Abcam
), rabbit
anti-Argonaute 2
(
1:800
;
ab5072
;
Abcam
),
mouse
anti-OCN (10 µg/mL;
ab13420
;
Abcam
), rabbit anti-OPG (
1:100
;
bs-20624R
;
Bioss
), rabbit
(3)
CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma
Molecular cancer
July 20, 2022
Liu L, Gu M, Ma J, Wang Y, Li M, Wang H, Yin X, Li X.
manufacturer’s instructions. Antibodies for RIP
assays
against Ago2 and IgG were purchased from
Abcam
(
ab5072
, Rabbit polyclonal antibody, Cambridge, MA, USA). Anti-m6A antibody (
A-1801
-020,
Epigentek
) and Magna
(4)
CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury
Cell Death Discovery
July 11, 2022
Jingjie Xiong, Yisen Hu, Yi Liu, Xiaocong Zeng
instructions. The antibodies against AGO2 and IgG used for the RIP
assays
were purchased from
Abcam
(
ab5072
, Cambridge, MA, USA).
Flow cytometry
Cardiomyocyte
apoptosis was measured using
flow cytometry
. After
(5)
CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p
Cancer Cell International
December 6, 2021
Yang Yang et al.
Magna RIP RNA-binding protein Immunoprecipitation Kit
(
Millipore
) according to the manufacturer’s instructions. Antibodies for RIP
assays
against Ago2 (
ab5072
) and IgG were purchased from
Abcam
, USA. In vivo
tumorigenesis
assay
Male nude
mice
(6 weeks old)
(6)
Circular RNA SERPINE2 promotes development of glioblastoma by regulating the miR-361-3p/miR-324-5p/BCL2 signaling pathway
Molecular Therapy - Oncolytics
September 24, 2021
Deheng Li, Liangdong Li, Xin Chen, Wentao Yang, Yiqun Cao
according to the producer’s protocol. RIP
assays
antibodies against AGO2 and IgG were obtained from
Abcam
(
ab5072
, rabbit polyclonal antibody, USA). In vivo
assays
for
tumor
growth To investigate
tumor
growth through
(7)
GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2
Oncology Letters
August 9, 2021
Hua Xu, Xiao Wang, Yinghong Zhang, Wei Zheng, Huijie Zhang
cellular lysates were next probed with magnetic beads conjugated with a
human
anti-AGO2 antibody (cat. no.
ab5072
; rabbit polyclonal antibody;
Abcam
) or control IgG (cat. no.
03-110
;
EMD Millipore
) at 4°C for 6 h.
(8)
circARL15 Plays a Critical Role in Intervertebral Disc Degeneration by Modulating miR-431-5p/DISC1
Frontiers in Genetics
June 21, 2021
Hanbang Wang, Yakun Zhu, Le Cao, Ziming Guo, Kai Sun, Wangbao Qiu, Haitao Fan
instructions. The antibodies against AGO2 and IgG used for the RIP
assays
were purchased from
Abcam
(
ab5072
, rabbit polyclonal antibody, Cambridge, MA, United States). Cell Counting Kit-8 (CCK-8)
Assay
At the
(9)
RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer
Cell transplantation
January 1, 2021
Yan X, Zhang M, Li B, Ji X, Wu H, Zhang Q.
manufacturer’s instructions. Antibodies for RIP
assays
against Ago2 and IgG were purchased from
Abcam
(
ab5072
, Rabbit polyclonal antibody, Cambridge, MA, USA). In vivo
Tumorigenesis
Assay
Male BALB/c nude
mice
(10)
Hsa_circ_0016760 exacerbates the malignant development of non‑small cell lung cancer by sponging miR‑145‑5p/FGF5
Oncology Reports
December 14, 2020
Zheng Zhu, Qiyong Wu, Ming Zhang, Jichun Tong, Bin Zhong, Kai Yuan
with RIP buffer containing magnetic beads conjugated with antibodies against AGO2 (
1:200
; product code
ab5072
) or IgG (
1:200
; product code
ab109489
; both from
Abcam
).
Wash buffer
was used to wash the beads. Subsequently,
(11)
circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2
Molecular Cancer
December 1, 2020
Lei Peng, Huaiming Sang, Shuchun Wei, Yuanyuan Li, Duochen Jin, Xudong Zhu, Xuan Li, Yini Dang, Guoxin Zhang
instructions. The antibodies against AGO2 and IgG used for the RIP
assays
were purchased from
Abcam
(
ab5072
, rabbit polyclonal antibody, Cambridge, MA, USA).
RNA pull-down
assay
The biotinylated circCUL2 probes
(12)
LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2
Aging
October 20, 2020
Jiang X, Chen D.
Magna RIP RNA-binding protein immunoprecipitation kit
(
Millipore
Corp., Billerica, MA, USA). We used rabbit polyclonal anti-Ago2 antibody (
ab5072
,
Abcam
) to
pull down
the RNA. Anti-IgG antibodies were used as negative control. Then, FAM181A-AS1 and
(13)
Knockdown of LncRNA DLEU2 Inhibits Cervical Cancer Progression via Targeting miR-128-3p
OncoTargets and Therapy
October 9, 2020
Bofei Wang, Jing Hang, Weiling Li, Wanqiong Yuan
guidance of the manufacturer. Antibodies for RIP
assays
against Ago2 and IgG were purchased from
Abcam
(
ab5072
, rabbit polyclonal antibody, Cambridge, MA, USA). Cell lysate was incubated with anti-Ago2 antibody
(14)
Circular RNA_CNST Promotes the Tumorigenesis of Osteosarcoma Cells by Sponging miR-421
Cell Transplantation
July 23, 2020
Ji-Hai Wang, Xue-Jian Wu, Yong-Zhuang Duan, Feng Li
manufacturer’s instructions. The antibodies against Ago2 and immunoglobulin G were purchased from
Abcam
(
ab5072
, Rabbit polyclonal antibody).
Animal Experiments
Six-week-old female immune-deficient nude
mice
(BALB/c-nu)
(15)
LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2
Cell Transplantation
July 9, 2020
Yuanjian Hui, Yan Yang, Deping Li, Juan Wang, Maojun Di, Shichao Zhang, Shasha Wang
protein G Sepharose beads (GE Healthcare, Eindhoven, the Netherlands) coated with anti-AGO2 antibody (
ab5072
;
Abcam
, Cambridge, MA, USA) overnight. Anti-IgG antibody was used as negative control. RNAs were isolated
(16)
CircSAMD4A regulates cell progression and epithelial‑mesenchymal transition by sponging miR‑342‑3p via the regulation of FZD7 expression in osteosarcoma
International Journal of Molecular Medicine
April 22, 2020
Chuhai Xie, Binwei Chen, Boyi Wu, Jianhong Guo, Yulong Shi, Yanming Cao
using RIP buffer, and then incubated with
human
anti-Argo-naute 2 (Ago2) antibody (
1:1,000
dilution,
ab5072
,
Abcam
) or
normal mouse IgG
(negative control,
1:1,000
dilution,
12-349
,
Sigma-Aldrich
; Merck KGaA).
(17)
Blocking lncRNA MALAT1/miR-199a/ZHX1 Axis Inhibits Glioblastoma Proliferation and Progression
Molecular therapy. Nucleic acids
December 6, 2019
Liao K, Lin Y, Gao W, Xiao Z, Medina R, Dmitriev P, Cui J, Zhuang Z, Zhao X, Qiu Y,
et al
.
and lysed in RIP lysis buffer with magnetic beads conjugated with
human
anti-Ago2 antibody (Cat. no:
ab5072
,
Abcam
) or IgG (Millipore, USA). The samples were incubated with Proteinase K and RNase
inhibitors
,
(18)
CircDLST promotes the tumorigenesis and metastasis of gastric cancer by sponging miR-502-5p and activating the NRAS/MEK1/ERK1/2 signaling
Molecular Cancer
December 1, 2019
Jing Zhang, Lidan Hou, Rui Liang, Xiaoyu Chen, Rui Zhang, Wei Chen, Jinshui Zhu
manufacturer’s instructions. Antibodies for RIP
assays
against Ago2 and IgG were purchased from
Abcam
(
ab5072
, Rabbit polyclonal antibody, Cambridge, MA, USA). RIP-
miRNA
expression profiling
The miRNAs were pulled-down
(19)
CircSLC3A2 functions as an oncogenic factor in hepatocellular carcinoma by sponging miR-490-3p and regulating PPM1F expression
Molecular Cancer
December 1, 2018
Hongjian Wang, Wei Chen, Ming Jin, Lidan Hou, Xiaoyu Chen, Rui Zhang, Jing Zhang, Jinshui Zhu
manufacturer’s instructions. Antibodies for RIP
assays
against Ago2 and IgG were purchased from
Abcam
(
ab5072
, Rabbit polyclonal antibody, Cambridge, MA, USA). Statistical analysis Statistical analyses were implemented
(20)
LncRNA SNHG4 promotes tumour growth by sponging miR-224-3p and predicts poor survival and recurrence in human osteosarcoma
Cell Proliferation
December 1, 2018
Ruida Xu, Fan Feng, Xiaosheng Yu, Zude Liu, Lifeng Lao
and IgG were purchased sion was upregulated in patients with TS ≥5 cm as compared with from
Abcam
(
ab5072
, rabbit polyclonal antibody). those with TS <5 cm (Figure 1B). 2.10 | Statistical analysis 3.2 |
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Reviews
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Anti-Argonaute-2 antibody from Abcam
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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