Anti-pan-AKT antibody ab8805 from Abcam

Product Specs
ItemAnti-pan-AKT antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab8805
Antibody TypePrimary
ClonalityPolyclonal
IsotypeIgG
Quantity200 UL
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Concentration75 mg/ml
Gene NameAKT1
ReactivityHuman, Mouse, Rat
Targetpan-AKT

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) MiR-18a-5p attenuates HER2-positive breast cancer development by regulating PI3K/AKT pathwayCancer Biology & TherapyDecember 31, 2023Yongjun Liu, Hua Yangwas done following 12% SDS-PAGE separation. Primary antibodies against p-AKT (ab38449, Abcam), AKT (ab8805, Abcam), P-PI3K (ab278545, Abcam), PI3K (ab32089, Abcam, UK), HER2 (ab134182, Abcam), and GAPDH (ab8245,

(2) Utilization of Cottonseed Meal Protein Hydrolysate by Crustaceans: Insights on Growth Performance, Protein Turnover, and Metabolism in Chinese Mitten Crab Eriocheir sinensisAnimals (Basel)November 23, 2023Chaofan He, Wenbin Liu, Ling Zhang, Weiliang Chen, Zishang Liu, Xiangyu Qian, Xiangfei Liwere insulin-like growth factor-1 (Igf-1, #AB182408, Abcam, Shanghai, China), protein kinase B, (Akt, #AB8805, Abcam, China), target of rapamycin (Tor, #AB134903, Abcam, China), forkhead box O1 (FoxO1, #AB52857,

(3) Oncogenic transmembrane protein 158 drives the PI3K/Akt signaling pathway to accelerate gastric cancer cell growthBrazilian Journal of Medical and Biological ResearchNovember 13, 2023Xiaoting Cui, Jie Lu, Cuijuan Zhao, Yu Duananti-p-PI3K (AF4372; 1:1000; Affinity Biosciences), anti-p-Akt (ab18206; 1:1000; Abcam, USA), anti-Akt (ab8805; 1:1000; Abcam), anti-Ki67 (ab16667; 1:1000; Abcam), and anti-β-actin (BM3873; 1:2000; Boster Biological

(4) CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathwayOncology LettersNovember 13, 2023Ping Tian, Jinglan Deng, Cailing Ma, Ainipa Miershali, Gulikezi Maimaitirexiati, Qi Yan, Yating Liu, Hatimihan Maimaiti, Yuting Li, Changhui Zhou, et al.anti-PI3K (1:100; cat. no. E-AB-22165; Elabscience Biotechnology, Inc.), anti-AKT (1:1,000; cat. no. ab8805; Abcam), p-AKT (1:100; cat. no. 4060; CST Biological Reagents Co., Ltd.), anti-E-cad (1:500; cat. no.

(5) Red Light‐Triggered Anti‐Angiogenic and Photodynamic Combination Therapy of Age‐Related Macular DegenerationAdvanced ScienceNovember 1, 2023Shuting Xu, Kaixuan Cui, Kaiqi Long, Jia Li, Ni Fan, Wai‐Ching Lam, Xiaoling Liang, Weiping Wanglisted as follows: CD31 antibody (1:500 dilution, GB11063, Servicebio, China), pan‐Akt antibody (1:1000, ab8805, Abcam, UK), pThr308‐Akt antibody (1:1000, ab38449, Abcam, UK), pThr180‐Tyr182‐MAPK antibody (1:1000,

(6) Utilizing network pharmacology and experimental validation to explore the potential molecular mechanisms of raw Pinellia ternate in treating esophageal cancerJournal of Gastrointestinal OncologyOctober 31, 2023Yanxin Liu, Yunfei Bai, Jinbang Zhang, Reginaldo Silva-Filho, Qingchun Zhu, Zhen Lei(glyceraldehyde-3-phosphate dehydrogenase) antibody: (1:5,000; ab8245, Abcam, Abcam, USA), Akt: (1:500; ab8805, Abcam, USA), p-Akt: (1:750; ab38449, Abcam, USA), p-GSK-3β (1:10,000; ab75814, Abcam, USA), and GSK-3β

(7) PCMT1 regulates the migration, invasion, and apoptosis of prostate cancer through modulating the PI3K/AKT/GSK-3β pathwayAging (Albany NY)October 27, 2023Jiacheng Zhong, Chao Yuan, Lin Liu, Yang Du, Yumin Hui, Zhiyuan Chen, Changhui Diao, Rui Yang, Guiyong Liu, Xiuheng LiuThe primary antibodies used were as follows: PCMT1 (10519-1-AP, Proteintech, dilution 1:100), AKT (Ab8805, Abcam, Cambridge, UK, dilution 1:500), P-AKT (Thr 308, Ab38449, Abcam, dilution 1:500), GSK-3β (AF5016,

(8) Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑BBiomedical ReportsOctober 26, 2023Qingwu Liao, Fang Li, Mingming Xue, Wenan Chen, Zhengang Tao, Yuejiao Song, Ying Yuananti-p-PI3K (1:1,000; cat. no. 17366; CST; Biological Reagents Co., Ltd.); anti-Akt (1:1,000; cat. no. ab8805; Abcam); anti-p-Akt (1:1,000; cat. no. ab81283; Abcam); anti-NF-κB1 (1:1,000; cat. no. ab288751; Abcam),

(9) The neuroprotective effects of Liuwei Dihuang medicine in the APP/PS1 mouse model are dependent on the PI3K/Akt signaling pathwayFrontiers in PharmacologyOctober 18, 2023Ye Yuan, Yamei Liu, Li Hao, Jinlian Ma, Simai Shao, Ziyang Yu, Ming Shi, Zhenqiang Zhang, Zijuan ZhangBOSTER, 1:800); Anti-Beclin1 (ab207612, Abcam, 1:1000); Anti-LC3 (ab192890, Abcam, 1:1000); Anti-p-Akt (ab8805, Abcam, 1:1000); Anti-β-actin (GB12001, Servicebio, 1:2000). 2.10 Statistics The data was processed

(10) Effects of Tiliroside and Lisuride Co-Treatment on the PI3K/Akt Signal Pathway: Modulating Neuroinflammation and Apoptosis in Parkinson’s DiseaseBiomedicinesOctober 9, 2023Faisal K. Alkholifi, Sushma Devi, Mohammed F. Aldawsari, Ahmed I. Foudah, Mohammed H. Alqarni, Mohamad Ayman Salkini, Sherouk Hussein SweilamKappa B ELISA Kit (MBS453975) were obtained from R&D Systems, Minneapolis, MN, USA. Anti-Akt Antibody (ab8805) was obtained from Abcam Inc., Cambridge, MA, USA. All other reagents used were of analytical grade. 2.2.

(11) Neuroprotective effect of Kurarinone against corticosterone‐induced cytotoxicity on rat hippocampal neurons by targeting BACE1 to activate P13K–AKT signaling – A potential treatment in insomnia disorderPharmacology Research & PerspectivesOctober 1, 2023Guoqing Wu, Yanyan Wu(ab191606, 1:1000, 84 kDa, Abcam, UK), phosphorylated (p)‐PI3K (ab182651, 1:1000, 84 kDa, Abcam, UK), AKT (ab8805, 1:500, 56 kDa, Abcam, UK), p‐AKT (ab38449, 1:500, 56 kDa, Abcam, UK), brain‐derived neurotrophic factor

(12) NOTCH2 gene mutation and gamma-secretase inhibitor in mediating the malignancy of ovarian cancerAging (Albany NY)September 30, 2023Wenjing Wang, Ruiqian Liu, Wei Liao, Landie Ji, Jie Mei, Dan SuCambridge), Hes1 (1:500; ab108937, Abcam, Cambridge), p-Akt (1:500; ab8933, Abcam, Cambridge), Akt (1:1000; ab8805, Abcam, Cambridge), Bcl-2 (1:500; ab182858, Abcam, Cambridge), Bax (1:500; ab32503, Abcam, Cambridge),

(13) Fibronectin promotes tumor progression through integrin αvβ3/PI3K/AKT/SOX2 signaling in non-small cell lung cancerHeliyonSeptember 14, 2023Jin-Long Wu, Cheng-Feng Xu, Xu-Hui Yang, Ming-Song Wangpolyclonal p-AKT (1:500, ab38449, Abcam, Cambridge, UK), rabbit polyclonal total-AKT (1:500, Abcam, ab8805, Cambridge, UK) and mouse monoclonal actin (1:500, ab8826, Abcam, Cambridge, UK). Next day, the sample

(14) Salvigenin Suppresses Hepatocellular Carcinoma Glycolysis and Chemoresistance Through Inactivating the PI3K/AKT/GSK-3β PathwayApplied Biochemistry and BiotechnologyAugust 1, 2023Hui Shao, Jingyan Chen, Ali Li, Lili Ma, Yongzhi Tang, Huazhong Chen, Yongping Chen, Junyan Liuserum for 30 min. We added the primary antibodies Anti-PI3K (ab191606, Abcam, 1:250) and Anti-AKT (ab8805, Abcam, 1:200) to incubate them overnight at 4 °C. Next, the fluorescence-labeled goat anti-rabbit IgG

(15) ERα promotes SUMO1 transcription by binding with the ERE and enhances SUMO1-mediated protein SUMOylation in breast cancerGland SurgeryJuly 31, 2023Quhui Wang, Nannan Zhang, Xiaobing Yang, Shichun Feng, Feiran Wang, Wei Zhang, Zhixian He(1/5,000, #ab52939, Abcam), myc (1/1,000, #ab32072, Abcam), GST (1/1,000, #ab111947, Abcam), p-Akt (1/500, #ab8805, Abcam), Akt (1/500, #ab38449, Abcam), p-ERK1/2 (1/1,000, #ab278538, Abcam), ERK1/2 (1/10,000, #ab184699,

(16) Neutrophil extracellular traps promote angiogenesis in gastric cancerCell communication and signaling : CCSJuly 21, 2023Yang S, Sun B, Li J, Li N, Zhang A, Zhang X, Yang H, Zou X.extracted, and the expression of ZO-1 (ab190085; Abcam), VE (ab33168; Abcam), AKT (ab38449; Abcam), p-AKT (ab8805; Abcam), mTOR, and p-mTOR (ab32028; Abcam) was analyzed via western blotting. Tubule formation experiment Matrigel

(17) SMYD3 drives the proliferation in gastric cancer cells via reducing EMP1 expression in an H4K20me3-dependent mannerCell death & diseaseJune 29, 2023Zeng Y, Ma G, Cai F, Wang P, Liang H, Zhang R, Deng J, Liu Y.53348S, 1:1000), rabbit anti-AKT1 (phosphor-S473) (Abcam, ab81283, 1:5000), rabbit anti-AKT (Abcam, ab8805, 1:5000), mouse anti-LaminB1 (proteintech, 66095, 1:1000) and mouse anti-GAPDH (Abcam, ab8245, 1:1000). Xenograft

(18) LINC00638 promotes the progression of non-small cell lung cancer by regulating the miR-541-3p/IRS1/PI3K/Akt axisHeliyonJune 15, 2023Juan Zhang, Yanhua Mou, Hui Li, Hui Shen, Jun Song, Qingfeng Licat. no. ab151549; 1:1,000), anti-p-Akt (Abcam; cat. no. ab38449; 1:1,000), anti-Akt (Abcam; cat. no. ab8805; 1:1,000), anti-cyclin D1 (Abcam; cat. no. ab16663; 1:1,000), anti-cyclin E1 (Abcam; cat. no. ab33911;

(19) Breviscapine regulates the proliferation, migration, invasion, and apoptosis of colorectal cancer cells via the PI3K/AKT pathwayScientific ReportsJune 14, 2023Pengfei Niu, Feng Liu, Fuming Lei, Jisheng Peng, Yanzhao Wang, Jun Zhao, Zhaoya Gao, Qingkun Gao, Jin Gup-PI3K (ab182651), 1:1000, Abcam), PI3K (ab191606, 1:1000, Abcam), p-AKT (ab8933, 1:500, Abcam), AKT (ab8805, 1:500, Abcam) and β-actin (ab8226, 1 µg/mL, Abcam) at 4 °C for 12 h. Next, the membranes were mixed

(20) Paritaprevir ameliorates experimental acute lung injury in vitro and in vivoArchives of Pharmacal ResearchJune 12, 2023Rui Ren, Xin Wang, Zehui Xu, Wanglin JiangCompany (Shanghai, PR China). Lipopolysaccharides (LPS), antibodies against VE-cadherin (ab33168), Akt (ab8805), p-Akt (ab38449), FOX-O1 (ab179450), p-FOX-O1 (ab259337), β-catenin (ab32572), claudin-5 (ab131259),

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8/8/2016A Nice Antibody For Total AKt Shikha SainiAkt is a key regulator in several cellular functions. it is phosphorylated and mediates the signals to nucleus of cell. Total AKT is often used as a control to determine relative phosphorylation of ... Read Review

Anti-pan-AKT antibody from Abcam

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Anti-pan-AKT antibody from Abcam

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