Anti-Ezrin antibody [3C12] ab4069 from Abcam

Product Specs
ItemAnti-Ezrin antibody [3C12]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab4069
Antibody TypePrimary
HostMouse, Nude mouse
ClonalityMonoclonal
IsotypeIgG1
Quantity10 UG, 100 UG
Clone3C12
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot
Conjugate/TagUnconjugated
Concentration0.1 mg/ml
Gene NameEZR
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetEzrin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) A Cantú syndrome mutation produces dual effects on KATP channels by disrupting ankyrin B regulationJournal of General PhysiologyJanuary 2, 2023Teresa Crespo-García, Marcos Rubio-Alarcón, Anabel Cámara-Checa, María Dago, Josu Rapún, Paloma Nieto-Marín, María Marín, Jorge Cebrián, Juan Tamargo, Eva Delpón, et al.Thermo Fisher Scientific), mouse monoclonal anti-AnkB (1:400; 33-3700; Invitrogen) and mouse monoclonal anti-ezrin (1:400; ab4069; Abcam) primary antibodies overnight at 4°C. All the antibodies had been validated by the manufacturers.

(2) Monoallelic loss of the F-actin-binding protein radixin facilitates startle reactivity and pre-pulse inhibition in miceFrontiers in Cell and Developmental BiologyNovember 28, 2022Torben J. Hausrat, Christian Vogl, Jakob Neef, Michaela Schweizer, Benjamin K. Yee, Nicola Strenzke, Matthias Kneussel#ab52495, IHC 1:200); mouse anti-γ-adaptin (BD Biosciences, #610386; WB 1:5,000); mouse anti-ezrin (Abcam, #ab4069, clone 3C12, WB 1:1,000); mouse anti-neuN (Millipore, clone A60, #MAB377, IHC 1:1,00). The following

(3) Mdm1 ablation results in retinal degeneration by specific intraflagellar transport defects of photoreceptor cellsCell death & diseaseSeptember 28, 2022Son Y, Kim SJ, Kim HY, Lee J, Kim JR.Rosemont, IL, USA); anti-γ-tubulin (sc-17787) (Santa Cruz Biotechnology, Dallas, TX, USA); anti-Ezrin (ab4069) (Abcam, Cambridge, UK); anti-acetylated α-tubulin (T6793), anti-Opsin (O4886) (Merck KGaA, Darmstadt,

(4) Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoidsSTAR protocolsSeptember 16, 2022Ryosaka M, Mae SI, Osafune K.AB_397580 Goat anti-E-CADHERIN (1:200),R&D,Cat#AF648;RRID: AB_355504 Mouse anti-EZRIN (1:500),Abcam,Cat#Ab4069;RRID: AB_304261 Mouse anti-FOXA1 (1:500),Santa Cruz,Cat#sc-514695 Rabbit anti-GATA3 (1:500),Cell Signaling,Cat#5852S;RRID:

(5) Long non-coding RNA taurine up regulated 1 promotes osteosarcoma cell proliferation and invasion through upregulating Ezrin expression as a competing endogenous RNA of micro RNA-377-3pBioengineeredJanuary 11, 2022Qin Yao, Yingchao Li, Yihua Pei, Bozhen XieChemilum HRP Substrate (Millipore). The primary antibodies for WB in this study included Ezrin antibody (#ab4069, Abcam, Cambridge, UK) and GAPDH antibody (#ab8245, Abcam). Dual-luciferase reporter gene assay Wild-type

(6) Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in miceNature CommunicationsDecember 1, 2021Yan Zhang et al.antibody (rabbit, 1:100, mAb#3726, Cell Signaling Technologies), anti-EZRIN antibody (rabbit, 1:100, ab4069, Abcam), anti-MOESIN antibody (rabbit, 1:100, ab52490, Abcam), and anti-GDF9 antibody (goat, 1:50, AF739,

(7) Molecular Mechanism of Stimulation of Na-K-ATPase by Leukotriene D4 in Intestinal Epithelial CellsInternational journal of molecular sciencesJuly 15, 2021Nepal N, Arthur S, Butts MR, Singh S, Palaniappan B, Sundaram U.(Alpha Innotech, San Leandro, CA, USA) and analyzed with its software. Ezrin antibody (Abcam, Cat# ab4069, Cambridge, MA, USA) and GAPDH (anti-mouse; ThermoFisher Scientific, Cat# MA-15378, Waltham, MA, USA)

(8) An inducible Cre mouse for studying roles of the RPE in retinal physiology and diseaseJCI insightMay 10, 2021Choi EH, Suh S, Einstein DE, Leinonen H, Dong Z, Rao SR, Fliesler SJ, Blackshaw S, Yu M, Peachey NS, et al.incubated with either an anti-RPE65 antibody (1:250; ref. 40) or an anti-ezrin antibody (1:400, Abcam, ab4069) in permeabilization solution containing 1% goat serum (Thermo Fisher Scientific) in PBS overnight at

(9) Mechanism of Na-K-ATPase Inhibition by PGE2 in Intestinal Epithelial CellsCellsMarch 29, 2021Niraj Nepal, Subha Arthur, Jennifer Haynes, Balasubramanian Palaniappan, Uma SundaramFluorChem instrument (Alpha Innotech, San Leandro, CA, USA) and analyzed with its software. Ezrin (Cat# ab4069, Abcam, Cambridge, MA, USA) antibody was used to normalize the expression levels of proteins in plasma membrane

(10) Dynamic conversion of cell sorting patterns in aggregates of embryonic stem cells with differential adhesive affinityBMC developmental biologyJanuary 6, 2021Tse JD, Moore R, Meng Y, Tao W, Smith ER, Xu XX.(Santa Cruz Biotechnology, sc-5279), anti-ZO1 (Invitrogen, Inc., #61–7300), anti-Ezrin (Abcam, [3C12] ab4069), anti-aPKC (Santa Cruz Biotechnology, In., sc-216), and anti-E-cadherin (BD Transduction Labs no. 610181). For

(11) Cancer Cell CD44 Mediates Macrophage/Monocyte-Driven Regulation of Head and Neck Cancer Stem CellsCancer researchOctober 1, 2020Gomez KE, Wu F, Keysar SB, Morton JJ, Miller B, Chimed TS, Le PN, Nieto C, Chowdhury FN, Tyagi A, et al.(#ab134047 or #ab174279 (peptide-blockade treated spheres) (Abcam, Cambridge, United Kingdom), 1:200 Ezrin (#ab4069 Abcam), and 1:100 PIK3CA (#PA5-77798 Thermo Fisher, Waltham, MA). Slides were coverslipped using Fluoroshield Mounting Medium

(12) Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear ConditioningCell reportsAugust 25, 2020Mazaré N, Oudart M, Moulard J, Cheung G, Tortuyaux R, Mailly P, Mazaud D, Bemelmans AP, Boulay AC, Blugeon C, et al.RRID:AB_11151389 Rabbit monoclonal anti-EEF1A1,Abcam,Cat#ab157455 Mouse monoclonal anti-Ezrin (3C12),Abcam,Cat#ab4069; RRID:AB_304261 Rabbit monoclonal anti-FTH1 (D1D4),Cell Signaling,Cat#4393S; RRID:AB_11217441 Rabbit

(13) PFOS ile tedavi edilen Sertoli hücre kültüründe ezrin ve fascin 1'in değerlendirilmesi: İn vitro bir çalışmaActa Medica AlanyaMarch 1, 2020Nazli Ece Gungor-Orduericultured Sertoli cells was performed as described [15]. Primary antibodies for Ezrin (Abcam cat no: ab4069, 1/200 dilution), fascin 1 (Abcam cat no: ab126722, 1/100) , F-actin (Phalloidin, sigma, p5282, 1/150

(14) A Direct Podocalyxin-Dynamin-2 Interaction Regulates Cytoskeletal Dynamics to Promote Migration and Metastasis in Pancreatic Cancer CellsCancer researchJune 1, 2019Wong BS, Shea DJ, Mistriotis P, Tuntithavornwat S, Law RA, Bieber JM, Zheng L, Konstantopoulos K.rabbit polyclonal antibody (Abcam, ab3457, 1:1000). 5) Ezrin (3C12) mouse monoclonal antibody (Abcam, ab4069, 1:500). 6) NHERF2 (D3A5) rabbit monoclonal antibody (Cell Signaling, 9568, 1:1000). 7) GST (26H1) mouse

(15) Wnt produced by stretched roof-plate cells is required for the promotion of cell proliferation around the central canal of the spinal cordDevelopmentJanuary 15, 2019Takuma Shinozuka, Ritsuko Takada, Shosei Yoshida, Shigenobu Yonemura, Shinji Takadathe following primary antibodies : anti-β-galactosidase (PM049, MBL International; 1:400), antiezrin (ab4069, Abcam; 1:400), anti-GFP (598, MBL International; 1:500), anti-Gpr177 (ab176376, Abcam; 1:400), anti-Ki67

(16) A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programingNature CommunicationsDecember 1, 2018Libing Mu et al.in media containing 25% of L929 supernatant. Reagents Anti-Moesin (ab151542) (1:1000), anti-Ezrin (ab4069) (1:200), anti-Radixin (ab52495) (1:1000), anti-phospho-ERM (ab76247) (1:1000), and anti-CD8α (ab209775)

(17) ERK phosphorylation functions in invadopodia formation in tongue cancer cells in a novel silicate fibre-based 3D cell culture systemInternational Journal of Oral ScienceDecember 1, 2018Masaharu Noi et al.Tween 20] containing 4% dried skim milk. As primary antibodies, anti-ezrin mouse antibody (1:1 000, ab4069; Abcam, Cambridge, UK), anti-β-actin mouse monoclonal antibody (1:1 000, sc-47778; Santa Cruz Biotechnology),

(18) Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transportMolecular biology of the cellJuly 1, 2018Muroyama A, Terwilliger M, Dong B, Suh H, Lechler T.Zymed/Invitrogen), rabbit anti-pericentrin (ab4448), rabbit anti-GRASP65 (ab30315), mouse anti-ezrin (ab4069), rabbit anti-Ki67 (ab15580; all from Abcam), mouse anti–γ-tubulin (clone GTU-88), mouse anti–β-actin

(19) miR-204 reverses temozolomide resistance and inhibits cancer initiating cells phenotypes by degrading FAP-α in glioblastomaOncology LettersMarch 20, 2018Yun‑Na Yang, Xiang‑Hua Zhang, Yan‑Ming Wang, Xi Zhang, Zheng Guanti-ras-related protein rab-22A (RAB22A; cat no. ab137093; 1:500; Abcam), rabbit anti-EZRIN (cat no. ab4069; 1:500; Abcam), rabbit anti-SRY-box 4 (SOX4; cat no. ab80261; 1:500; Abcam), rabbit anti-activating

(20) Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testisCell death & diseaseFebruary 12, 2018Wen Q, Li N, Xiao X, Lui WY, Chu DS, Wong CKC, Lian Q, Ge R, Lee WM, Silvestrini B, et al.,Santa Cruz Biotechnology,sc-20770,1:200,— Fascin(AB_627580),Mouse,Santa Cruz Biotechnology,sc-21743,1:200,— Ezrin(AB_304261),Mouse,Abcam,ab4069,1:300,— β1-integrin(AB_2130101),Rabbit,Santa Cruz Biotechnology,sc-8978,1:200,1:100 α-tubulin(AB_2241126),Mouse,Abcam,ab7291,1:1000,1.200 Detyrosinated

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Anti-Ezrin antibody [3C12] from Abcam

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Anti-Ezrin antibody [3C12] from Abcam

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