Anti-MAP2 antibody ab5392 from Abcam

Product Specs
ItemAnti-MAP2 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab5392
Antibody TypePrimary
IsotypeIgY
HostChicken
Quantity25 UL
ApplicationsImmunocytochemistry, Western blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
FormatLipid extraction from egg yolk, followed by ammonium sulphate precipitation
Concentration19 mg/ml
Research AreaNeuroscience
Gene Namemap2
ReactivityMouse, Rat
TargetMAP2

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Developing a human iPSC-derived three-dimensional myelin spheroid platform for modeling myelin diseasesiScienceNovember 17, 2023Lizhao Feng, Jianfei Chao, Mingzi Zhang, Elizabeth Pacquing, Weidong Hu, Yanhong Shimonocolonal anti-MBP,Millipore Sigma,Cat# MAB386; RRID: AB_94975 Chicken polyclonal anti-Map2,Abcam,Cat# ab5392; RRID: AB_2138153 Rabbit polyclonal anti-OLIG2,Millipore Sigma,Cat# AB9610; RRID: AB_570666 Rabbit

(2) A tetracationic porphyrin with dual anti-prion activityiScienceSeptember 15, 2023Antonio Masone, Chiara Zucchelli, Enrico Caruso, Giada Lavigna, Hasier Eraña, Gabriele Giachin, Laura Tapella, Liliana Comerio, Elena Restelli, Ilaria Raimondi, et al.(WUR; 1:500), rabbit polyclonal anti-LAMP1 (Abcam ab24170; 1:1000), chicken polyclonal anti-MAP2 (Abcam ab5392; 1:50000) primary antibodies diluted in blocking solution, for 1h at RT, or overnight at 4°C, then rinsed

(3) Functional imaging of conduction dynamics in cortical and spinal axonsElifeAugust 22, 2023Milos Radivojevic, Anna Rostedt PungaSigma-Aldrich) for 30 min to prevent unspecific binding of antibodies. The primary antibodies Anti-Map2 (Abcam, ab5392), Anti-β-III tubulin (Abcam, ab18207), and Anti-mCherry (Abcam, ab125096), diluted in phosphate buffered

(4) Extracellular RNAs-TLR3 signaling contributes to cognitive impairment after chronic neuropathic pain in miceSignal Transduct Target TherAugust 7, 2023Xueying Zhang, Rui Gao, Changteng Zhang, Yi Teng, Hai Chen, Qi Li, Changliang Liu, Jiahui Wu, Liuxing Wei, Liyun Deng, et al.follows: anti-TLR3 rabbit polyclonal antibody (1:200, ab62566, Abcam), anti-MAP2 chicken antibody (1:200, ab5392, Abcam), anti-NeuN mouse antibody (1:100, 26975, Proteintech), anti-GFAP mouse antibody (1:200, 60190-1-Ig,

(5) Schizophrenia Risk Mapping and Functional Engineering of the 3D Genome in Three Neuronal SubtypesbioRxivJuly 22, 2023Samuel K. Powell, Will Liao, Callan O’Shea, Sarah Kammourh, Sadaf Ghorbani, Raymond Rigat, Rahat Elahi, PJ Michael Deans, Derek J. Le, Poonam Agarwal, et al.Antibody,Species,Supplier,Product number,Dilution MAP2,Ck,Abcam,ab5392,1:500 SYNAPSIN,Ms,Synaptic Systems,106 011,1:500 TUJ1,Ms,BioLegend,MMS-435P,1:1000 Alexa 568 anti-Mouse,Ms,Life technologies

(6) Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cellsiScienceJuly 21, 2023Martin H. Berryer, Matthew Tegtmeyer, Loïc Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietiläinen, et al.SYNAPSIN1 (1:1000, Millipore, AB1543, RRID:AB_2200400) and Chicken anti-human MAP2 (1:1000, Abcam, ab5392, RRID:AB_2138153) primary antibodies, Goat anti-chicken AlexaFluor 488 (1:1000, ThermoFisher, A21131),

(7) Detection of endogenous NPY release determined by novel GRAB sensor in cultured cortical neuronsFrontiers in Cellular NeuroscienceJuly 20, 2023Emma Kragelund Christensen, Ainoa Konomi-Pilkati, Joscha Rombach, Raquel Comaposada-Baro, Huan Wang, Yulong Li, Andreas Toft SørensenC-terminal region of full-length NPY (1:1000; N9528, Sigma-Aldrich, Germany) and chicken anti-MAP2 (1:500, Ab5392, Abcam) in 5% goat serum + 0.25% Triton X-100 (Sigma-Aldrich, Germany, #93443) at 4°C overnight. The

(8) AraC interacts with p75NTR transmembrane domain to induce cell death of mature neuronsCell Death & DiseaseJuly 17, 2023Vanessa Lopes-Rodrigues, Pia Boxy, Eunice Sim, Dong Ik Park, Michael Habeck, Josep Carbonell, Annika Andersson, Diana Fernández-Suárez, Poul Nissen, Anders Nykjær, et al.(Neuromics; GT15057; 1:500), polyclonal anti-TrkB (R&D systems; AF1494; 1:250), polyclonal anti-MAP2 (Abcam; ab5392; 1:2000) and polyclonal anti-P65NFkB (Santa Cruz; sc-372; 1:250). Secondary antibodies were Alexa Fluor–conjugated

(9) Absent in melanoma 2 mediates aging‐related cognitive dysfunction by acting on complement‐dependent microglial phagocytosisAging CellJuly 1, 2023Lei Ye, Shu Shu, Junqiu Jia, Min Sun, Siyi Xu, Xinyu Bao, Huijie Bian, Yi Liu, Meijuan Zhang, Xiaolei Zhu, et al.Bio Rad, MCA1957GA); mouse anti‐PSD‐95 (1:1000, Abcam, ab2723); chicken anti‐MAP‐2 (1:1000, Abcam, ab5392); rat anti‐C1q (1:500, Abcam, ab11861); rat anti‐C3 (1:500, Abcam, ab11862). The samples were washed

(10) Co-Administration of Resolvin D1 and Peripheral Nerve-Derived Stem Cell Spheroids as a Therapeutic Strategy in a Rat Model of Spinal Cord InjuryInternational Journal of Molecular SciencesJune 30, 2023Seung-Young Jeong, Hye-Lan Lee, SungWon Wee, HyeYeong Lee, GwangYong Hwang, SaeYeon Hwang, SolLip Yoon, Young-Il Yang, Inbo Han, Keung-Nyun KimThese primary antibodies included mouse anti-CNPase (1:200; Ab6319; Abcam), chicken anti-MAP2 (1:800; Ab5392; Abcam), and goat anti-GFAP (1:800; Ab53554; Abcam). After incubation, the tissue samples were washed

(11) Structurally and Morphologically Distinct Pathological Tau Assemblies Differentially Affect GVB AccumulationInternational Journal of Molecular SciencesJune 29, 2023Marta Jorge-Oliva, Jan R. T. van Weering, Wiep ScheperTriton X-100 in PBS. Incubation with CK1δ (Thermo-Scientific Cat# PA5-32129, 1:500), MAP2 (Abcam Cat# ab5392, 1:250), and MC1 (a kind gift from Dr. Peter Davies, 1:500) primary antibodies was performed O/N at

(12) Single-cell RNA-sequencing identifies various proportions of excitatory and inhibitory neurons in cultured human fetal brain cortical tissuesFrontiers in NeuroscienceJune 28, 2023Rong Liu, Wei Dong, Dan Xiong, Lanqi Hu, Haoran Zhang, Xiaoping Yuan, Zhonghui Tang, Fang Fu, Xin Yang, Xia Wugoat serum, and 0.3% Triton X-100. We used primary antibodies against MAP2 (Chicken, 1:1,500; Abcam ab5392; RRID: AB_2138153), GABA (Rabbit, 1:500; sigma, A2052: RRID: AB_477652), and NEUROD2 (rabbit, 1:300;

(13) Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoidsNature communicationsJune 24, 2023Sebastian R, Jin K, Pavon N, Bansal R, Potter A, Song Y, Babu J, Gabriel R, Sun Y, Aronow B, et al.(1:1000, EMD Milipore ABN78), rabbit anti-S100B (1:1000, Sigma S2644), chicken anti-MAP2 (1:5000, Abcam ab5392), rabbit anti-SYNAPTOPHYSIN (1:1000, Abcam ab14692), rabbit anti-HOMER (1:1000 Synaptic System 160003),

(14) Postoperative risk of IDH mutant glioma–associated seizures and their potential management with IDH mutant inhibitorsJournal of Clinical InvestigationJune 15, 2023Michael R. Drumm, Wenxia Wang, Thomas K. Sears, Kirsten Bell-Burdett, Rodrigo Javier, Kristen Y. Cotton, Brynna T Webb, Kayla T Byrne, Dusten Unruh, Vineeth Thirunavu, et al.used were as follows: NeuN (Abcam, 1B7, ab104224; 1:400), cFOS (Abcam, ab190289; 1:500), MAP2 (Abcam, ab5392; 1:1,000), GFAP (IHC: Biocare, CP040A, 1:500; immunofluorescent: Abcam, ab5804, 1:1,000), phospho-S6

(15) Emergent structural and functional properties of hippocampal multi-cellular aggregatesFrontiers in NeuroscienceJune 15, 2023Victor P. Acero, Suradip Das, Olivia Rivellini, Erin M. Purvis, Dayo O. Adewole, Daniel Kacy CullenIII (1:500; T8578, Sigma-Aldrich) to label axons, microtubule associated protein-2 (MAP2; 1:10000; AB5392, ABCAM) to label neuronal somata and dendrites, and glial-fibrillary associated protein (GFAP; 1:500;

(16) Lipid nanoparticle delivery limits antisense oligonucleotide activity and cellular distribution in the brain after intracerebroventricular injectionMolecular Therapy - Nucleic AcidsJune 13, 2023Amy E. Byrnes, Sara L. Dominguez, Chun-Wan Yen, Benjamin I. Laufer, Oded Foreman, Mike Reichelt, Han Lin, Meredith Sagolla, Kathy Hötzel, Hai Ngu, et al.rat anti-ASO (Roche) 1:10,000, mouse anti-MAPT (MAB361, Millipore Sigma) 1:200, chicken anti-MAP2 (ab5392, Abcam) 1:10,000, mouse anti-⍺-tubulin (DM1⍺, T6199, Millipore Sigma) 1:1,000, rabbit anti-Iba1 (019–19741,

(17) Selective Inhibition of Soluble Tumor Necrosis Factor Alters the Neuroinflammatory Response following Moderate Spinal Cord Injury in MiceBiologyJune 12, 2023Lund MC, Ellman DG, Nielsen PV, Raffaele S, Fumagalli M, Guzman R, Degn M, Brambilla R, Meyer M, Clausen BH, et al.combinations overnight at 4 °C: chicken anti-MAP2 (Microtubule-associated protein 2, 1:100, Abcam, cat. no. ab5392), rabbit anti-TNFR1 (1:50, clone H-271, Santa Cruz, cat. no. sc-7895, Århus, Denmark), rabbit anti-TNFR2

(18) SARS-CoV-2 infection and viral fusogens cause neuronal and glial fusion that compromises neuronal activityScience advancesJune 9, 2023Martínez-Mármol R, Giordano-Santini R, Kaulich E, Cho AN, Przybyla M, Riyadh MA, Robinson E, Chew KY, Amor R, Meunier FA, et al.blocked in 5% (w/v) BSA for 5 hours. The organoids were then incubated with primary antibodies to MAP2 (#ab5392, Abcam) at 4°C for 3 days. Following PBS washing, they were incubated with Alexa Fluor–conjugated secondary

(19) Differentiation, Transcriptomic Profiling, and Calcium Imaging of Human Hypothalamic NeuronsCurrent ProtocolsJune 5, 2023Hsiao‐Jou Cortina Chen, Simone Mazzaferro, Tian Tian, Iman Mali, Florian T. MerkleRRID:AB_2064130",Rat,1:500 FOXG1,"Abcam, cat. no. ab196868, lot no. RRID:AB_2892604",Rabbit,1:100 MAP2,"Abcam, cat. no. ab5392, lot no. RRID:AB_2138153",Chicken,1:2000 NKX2.1,"Thermo Fisher Scientific, cat. no. 18-0221, lot. no

(20) Treadmill exercise exerts a synergistic effect with bone marrow mesenchymal stem cell-derived exosomes on neuronal apoptosis and synaptic-axonal remodelingNeural regeneration researchJune 1, 2023Jiang XH, Li HF, Chen ML, Zhang YX, Chen HB, Chen RH, Xiao YC, Liu N.anti-synaptophysin (1:1000, Abcam, Cat# ab32127, RRID: AB_2286949), chicken anti-MAP-2 (1:1000, Abcam, Cat# ab5392, RRID:AB_2138153), rabbit anti-GAP-43 (1:800, Abcam, Cat #ab75810, RRID: AB_1310252), rabbit anti-β-actin

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12/29/2016Best Antibody For MAP2 Tara VanderweydeDiscovered this antibody when needed to get a MAP2 antibody quick that was not mouse or rabbit. Very concentrated and works great at 1:10,000. Best for immunostaining primary neurons that I have tried... Read Review

Anti-MAP2 antibody from Abcam

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Anti-MAP2 antibody from Abcam

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