Fig 1: Confocal imaging of immunohistochemistry analysis of ß-catenin and NICD1 in colon cancer tissuesRepresentative image of ß-catenin and NICD1 localization in colon cancer tissue samples. DAPI, Merged image of NICD1 and ß-catenin and HE stain. The antibody used (ab8925, from Abcam) actually recognizes the active form of Notch1 receptor, exposed after cleavage by ?-secretase. Yellow bar indicates 50um. (A) Nuclear NICD1 positive pattern: NICD1 and ß-catenin were expressed in the nucleus of colon cancer cell. Merged image indicated that NICD1 and ß-catenin were co-expressed in the same colon cancer cell. (B) Nuclear NICD1 negative pattern: NOTCH1 and ß-catenin were expressed in the cytoplasm of colon cancer cell. Merged image indicated that NOTCH1 and ß-catenin were co expressed in the same colon cancer cell.
Supplier Page from Abcam for Anti-activated Notch1 antibody