Anti-alpha Tubulin antibody [TU-01] ab7750 from Abcam

Product Specs
ItemAnti-alpha Tubulin antibody [TU-01]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab7750
Antibody TypePrimary
HostMouse, Nude mouse
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ClonalityMonoclonal
IsotypeIgG1
CloneTU-01
Quantity100 UG
Conjugate/TagUnconjugated
FormatPurified from ascites using precipitation methods
Concentration1 mg/ml
Gene NameTUBA4A
ReactivityHuman, Mouse
Targetalpha Tubulin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Staphylococcus aureus phagocytosis is affected by senescenceFrontiers in AgingJuly 31, 2023Esteban Robledo, Paula Guadalupe Benito Rodriguez, Israel Aníbal Vega, María Isabel Colombo, Milton Osmar Aguileramouse anti-LAMP2 (Santa Cruz Biotechnology, # sc-18822, RRID:AB_626858), mouse anti-tubulin (Abcam # ab7750, RRID:AB_306044), and mouse monoclonal anti-actin (Sigma-Aldrich, # A5441, RRID:AB_476744). The secondary

(2) The combination of Astragalus membranaceus and ligustrazine mitigates cerebral ischemia‐reperfusion injury via regulating NR2B‐ERK/CREB signalingBrain and BehaviorFebruary 1, 2023Xialing Tang, Shanshan Xie, Huajun Wang, Yingbin Li, Zhiyu Lai, Shuangxi Sun, Ruanhuan Pan, Yan Huang, Jun Caiab184699, Abcam), p‐ERK1/2 (cat. no. ab201015, Abcam), and β‐actin (cat. no. ab6276, Abcam) and α‐tubulin (ab7750, Abcam). After incubation with horseradish peroxidase‐labeled secondary antibodies (NO.32260, Goat anti‐Rabbit IgG [H+L

(3) Characterization of human nasal organoids from chronic rhinosinusitis patientsBiology openAugust 15, 2022Ramezanpour M, Bolt H, Hon K, Shaghayegh G, Rastin H, Fenix KA, Psaltis Alkis J, Wormald PJ, Vreugde S.USA), rabbit Anti-MUC2 antibody (ab90007, Abcam, Cambridge, UK), mouse anti-alpha Tubulin antibody (ab7750, Abcam, Cambridge, UK), mouse Anti-E-cadherin (610182, BD BioSciences, CA, USA) or mouse monoclonal

(4) Focal Adhesion Protein Vinculin Is Required for Proper Meiotic Progression during Mouse SpermatogenesisCellsJune 23, 2022Petrusová J, Havalda R, Flachs P, Venit T, Darášová A, Hůlková L, Sztacho M, Hozák P.Cell Signaling Technology, Danvers, MA, USA), rabbit anti REC8 (1:400, ab149221 Abcam), mouse anti αTubulin (1:100, ab7750 Abcam), rabbit anti-PSMB1 (1:100, PA5–49648 Thermo Fisher), and rabbit anti-Sumo2 + Sumo3 (1:200, ab3742

(5) Factor quinolinone inhibitors disrupt spindles and multiple LSF (TFCP2)-protein interactions in mitosis, including with microtubule-associated proteinsPLOS ONEJune 15, 2022Sarah A. Yunes et al.rabbit polyclonal anti-α-tubulin (1:5000, Thermo Fisher PA529444), and anti-α-tubulin antibody (Abcam #AB7750). Secondary antibodies included: goat anti-mouse IgG-Texas Red (1:200, Thermo Fisher T-862), goat anti-mouse

(6) Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAsNature cell biologySeptember 1, 2021Loubalova Z, Fulka H, Horvat F, Pasulka J, Malik R, Hirose M, Ogura A, Svoboda P.overnight. To visualize the meiotic spindle, MII oocytes were stained with mouse anti-Tubulin (Abcam, ab7750) diluted 1:100 for 1 h at room temperature. To visualize H3K9me3 histone modification, rabbit anti-H3K9me3

(7) Signs of Chronic Hypoxia Suggest a Novel Pathophysiological Event in α‐Synucleinopathies Movement DisordersDecember 1, 2020Antonio Heras‐Garvin, Christoph Danninger, Sabine Eschlböck, Janice L. Holton, Gregor K. Wenning, Nadia Stefanova(Cell Signaling; 7096), anti‐HIF1α (BD; 610959), anti‐GAPDH (Sigma; G9545), anti‐α‐Tubulin (Abcam; ab7750), and anti‐ß‐tubulin (Sigma; T4026) as loading controls. Signal detection was performed using horseradish

(8) Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 GeneJournal of neuropathology and experimental neurologyJanuary 1, 2020Katsumata Y, Fardo DW, Bachstetter AD, Artiushin SC, Wang WX, Wei A, Brzezinski LJ, Nelson BG, Huang Q, Abner EL, et al.Lee and produced originally by Dr Peter Davies, 1:1000 dilution), and anti-α-Tubulin (TU-01, Cat no.: ab7750, Abcam, 1:100 dilution). Real-Time-qPCR Analysis of Human Brain Samples to Test If the VNTR Polymorphism

(9) Mitochondrial dysfunction causes Ca²⁺overload and ECM degradation–mediated muscle damage inC. elegansThe FASEB JournalAugust 1, 2019Surabhi Sudevan, Mai Takiura, Yukihiko Kubota, Nahoko Higashitani, Michael Cooke, Rebecca A. Ellwood, Timothy Etheridge, Nathaniel J. Szewczyk, Atsushi Higashitanimembrane was incubated overnight at 4°C in primary antibody (anti-mCherry, ab125096, anti-α-tubulin, ab7750; Abcam, Cambridge, MA, USA) prepared in 2.5% milk. This was followed by 1 wash with 1-time PBS with

(10) Glycocalyx Remodeling with Glycopolymer-Based Proteoglycan MimeticsMethods in Molecular BiologyMay 28, 2019Mia L. Huang, Raymond A. A. Smith, Greg W. Trieger, Kamil Godula(Cell Signaling Technology cat. #9102 used at 1:6000 for Western blot). Anti-alpha-tubulin (Abcam cat. # ab7750, used at 1:1000 for Western blot). Anti-rabbit HRP (Cell Signaling Technology). Anti-mouse HRP (Cell

(11) Anti-proliferative effect of isorhamnetin on HeLa cells through inducing G2/M cell cycle arrestExperimental and Therapeutic MedicineFebruary 26, 2018Juan Wei, Hailan Su, Yang Bi, Jixin Li, Lidan Feng, Wenjun Shengfrom Cell Signaling Technology, Inc. (Danvers, MA, USA). Primary antibodies for α-tubulin (cat. no. ab7750) and β-tubulin (cat. no. ab70187) were obtained from Abcam (Cambridge, MA, USA) and used at dilution

(12) Progressive striatonigral degeneration in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapiesActa neuropathologica communicationsJanuary 3, 2018Refolo V, Bez F, Polissidis A, Kuzdas-Wood D, Sturm E, Kamaratou M, Poewe W, Stefanis L, Angela Cenci M, Romero-Ramos M, et al.were then stripped and re-probed with β-actin (1:120,000; Sigma-Aldrich) or mouse anti-alpha-tubulin (ab7750, Abcam). The optical density of specific bands was then normalized to the corresponding β-actin or alpha-tubulin

(13) A novel Fer/FerT targeting compound selectively evokes metabolic stress and necrotic death in malignant cellsNature CommunicationsDecember 1, 2017Yoav Elkis et al.mAB8164 1:500), anti-lactate dehydrogenase (Cell Signaling mAB3582, USA, 1:1000), anti-Tubulin (Abcam ab7750, USA, 1:1000), anti-poly [ADP-ribose] polymerase 1 (PARP-1) (Santa-Cruz sc-8007, USA, 1:1000), anti-poly-ADP-ribosylation

(14) Epigenetic modifications promote the expression of the orphan nuclear receptor NR0B1 in human lung adenocarcinoma cellsOncotargetJuly 12, 2016Yongjie Lu et al.and mouse monoclonal antibodies for H3K4me3 (ab6000), H3K9me2 (ab1220), H3K27me3 (ab6002), α-TUBULIN (ab7750) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, ab9482) were obtained from Abcam (Cambridge, MA).

(15) A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathwaysScientific reportsMay 9, 2016Moshfegh C, Aires L, Kisielow M, Vogel V.temperature for secondary antibodies. The primary antibodies were anti-alpha-tubulin (1:1000, Abcam, ab7750), anti-Cyclin D1 (1:1000, Cell Signaling Technology, 2926), anti-Cyclin B1 (1:1000, Cell Signaling Technology,

(16) Heme Oxygenase-1 Suppresses Bovine Viral Diarrhoea Virus Replication in vitroScientific ReportsDecember 1, 2015Chong Zhang et al.1: 1000 (Abcam, catalog#: ab90492, Cambridge, UK), anti-α-tubulin antibody at a 1:5000 (Abcam, catalog#: ab7750, Cambridge, UK), and then with HRP-conjugated anti-mouse IgG at a 1:2000 (Jackson, catalog#: 111-035-003,

(17) The combination of rapamycin and resveratrol blocks autophagy and induces apoptosis in breast cancer cellsJournal of cellular biochemistryMarch 1, 2015Alayev A, Berger SM, Kramer MY, Schwartz NS, Holz MK.9665); PDCD4 (Proteintech, 12587–1-AP), actin (Santa Cruz Biotechnology, sc-1615), α-tubulin (Abcam, ab7750), and PARP (Abcam, ab32071). Blots were incubated with IRDye-conjugated anti-rabbit (LI-COR, 827–08365),

(18) Fluorescence nanoscopy by polarization modulation and polarization angle narrowingNature MethodsMay 1, 2014Nour Hafi et al.with 5% (w/v) BSA in PBS. α-tubules were immunolabeled with anti–α-tubulin antibody from mouse (Abcam, ab7750, dilution ~1:100). For samples in Figure 3a,b the primary antibody was labeled with Alexa Fluor 488

(19) GATA6 is a crucial regulator of Shh in the limb budPLoS geneticsJanuary 1, 2014Kozhemyakina E, Ionescu A, Lassar AB.by Western blot, and the membranes probed with goat anti-Gli3 (AF3690, R&D systems) and mouse anti-alpha Tubulin (ab7750, Abcam) antibodies. Whole-mount in situ hybridization, cartilage and bone staining Embryos were fixed

(20) Posttranslational Modifications of Tubulin and the Polarized Transport of Kinesin-1 in NeuronsMolecular Biology of the CellFebruary 15, 2010Jennetta W. Hammond, Chun-Fang Huang, Stefanie Kaech, Catherine Jacobson, Gary Banker, Kristen J. VerheyIA), total α-tubulin (monoclonal DM1α [Sigma-Aldrich, St. Louis, MO]; polyclonal ab18251 or monoclonal ab7750 [Abcam, Cambridge, MA]), acetylated α-tubulin (monoclonal 6-11B-1 or a rabbit polyclonal antibody generated

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Anti-alpha Tubulin antibody [TU-01] from Abcam

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Anti-alpha Tubulin antibody [TU-01] from Abcam

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