Anti-CUG-BP1 antibody [3B1] ab9549 from Abcam

Product Specs
ItemAnti-CUG-BP1 antibody [3B1]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab9549
Antibody TypePrimary
Quantity10 UG, 100 UG
IsotypeIgG1
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostMouse, Nude mouse
ClonalityMonoclonal
Clone3B1
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameCELF1
ValidationKnockOut Validated
ReactivityHuman
TargetCUG-BP1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Senescence plays a role in myotonic dystrophy type 1JCI insightOctober 10, 2022García-Puga M, Saenz-Antoñanzas A, Gerenu G, Arrieta-Legorburu A, Fernández-Torrón R, Zulaica M, Saenz A, Elizazu J, Nogales-Gadea G, Gadalla SM, et al.Santa Cruz Biotechnology; 1:25, MANDM4 and MANDM13, DSHB), anti-MBNL1 (1:500, ab45899; Abcam), anti-CUGBP1 (1:500, ab9549; Abcam), anti–pH3 Ser10 (1:2000, ab14955; Abcam), anti-p27KIP1 (1:500, BD610241; BD Biosciences), anti-p16INK4A

(2) Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulationNature communicationsSeptember 1, 2021Hoefig KP, Reim A, Gallus C, Wong EH, Behrens G, Conrad C, Xu M, Kifinger L, Ito-Kureha T, Defourny KAY, et al.min, 10,000 × g, 4 °C). Rabbit anti-roquin (A300-514A; Bethyl, 6 µg) and mouse monoclonal anti-Celf1 (ab9549; Abcam, 2 µg) were bound to 50 µl protein A (10001D, Invitrogen) or G (10004D, Invitrogen) magnetic

(3) Whole mount staining of lenses for visualization of lens epithelial cell proteinsMethodsXMay 6, 2021Shaili D. Patel, Sandeep Aryal, Lucas P. Mennetti, Justin ParrenoAbnormal,Abcam,Ab200342,1:100 Abcam,Ab200342,1:100,Vision-Like1 (ElavL1/Hur) CUGBP Elav-Like Family Member 1 (Celf1),Abcam,Ab9549,1:250 Alpha smooth muscle actin,Abcam,Ab7817,1:100 Goat anti rabbit IgG Alexa Fluor® 488,Invitrogen,A-11008,1:100 Goat

(4) Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver DevelopmentInternational journal of molecular sciencesDecember 7, 2020Suzuki T, Adachi S, Kikuguchi C, Shibata S, Nishijima S, Kawamoto Y, Iizuka Y, Koseki H, Okano H, Natsume T, et al.IMP3 (RN009P) were from MBL (Nagoya, Japan). Antibodies against AFP (ab46799), AUF1 (ab61193), CELF1 (ab9549), and CSDE1 (ab201688) were obtained from Abcam (Cambridge, UK). Anti-Albumin antibody (sc-46291) was

(5) Proteomics-Based Characterization of miR-574-5p Decoy to CUGBP1 Suggests Specificity for mPGES-1 Regulation in Human Lung Cancer CellsFrontiers in PharmacologyMarch 13, 2020Anne C. Emmerich, Julia Wellstein, Elena Ossipova, Isabell Baumann, Johan Lengqvist, Kim Kultima, Per-Johan Jakobsson, Dieter Steinhilber, Meike J. Saulnear-infrared fluorescence. The membranes were incubated with primary antibodies that recognize CUGBP1 (ab9549, Abcam), SMAD2 (sc-6200, Santa Cruz), SMAD3 (ab28379, Abcam), SMAD4 (ab3219, Abcam), p38 (sc-535, Santa Cruz

(6) Quantitative Studies of Muscleblind Proteins and Their Interaction With TCF4 RNA Foci Support Involvement in the Mechanism of Fuchs' DystrophyInvestigative Opthalmology & Visual ScienceSeptember 28, 2019Ziye Rong, Jiaxin Hu, David R. Corey, V. Vinod Moothawere: MBNL1 (Santa Cruz sc-47740 or EMD Millipore ABE241), MBNL2 (Santa Cruz sc-136167), CUGBP1 (Abcam ab9549), TCF4 (Abnova H00006925-M03), tubulin (Cell Signaling 3873P), GAPDH (G9545 Sigma), and Histone H3 (Cell Signaling

(7) The MBNL/CELF Splicing Factors Regulate Cytosolic Sulfotransferase 4A1 Protein Expression during Cell DifferentiationDrug metabolism and disposition: the biological fate of chemicalsMarch 1, 2019Idris M, Butcher NJ, Minchin RF.anti-SULT4A1 (12578-1-AP; ProteinTech Group, Chicago, IL), anti–MBNL-1 (ab108519; Abcam), anti–CELF-1 (ab9549; Abcam), anti–MBNL-2 (sc-136167; Santa Cruz Biotechnology, Dallas, TX), anti–CELF-2 (sc-47731; Santa Cruz Biotechnology

(8) The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens developmentPLOS GeneticsMarch 22, 2018Archana D. Siddam et al.

(9) Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1Nature CommunicationsDecember 21, 2017Metehan Cifdaloz et al.(Zeiss). Immunofluorescence Paraffin-embedded malignant melanoma tissues were processed for dual immunostaining of CELF1 (Abcam, ab9549) and DEK (Abcam, ab166624). Antigen retrieval was performed using 10 mmol/L sodium citrate buffer at

(10) Developmentally regulated alternative splicing is perturbed in type 1 diabetic skeletal muscleMuscle & NerveOctober 1, 2017Curtis A. Nutter, Elizabeth Jaworski, Sunil K. Verma, Yareli Perez‐Carrasco, Muge N. Kuyumcu‐Martinezantibodies used in WB analyses were as follows: rabbit anti-RBFOX1 (HPA040809; Sigma); mouse anti-CELF1 (ab9549; Abcam, Cambridge, Massachusetts); mouse anti-MBNL1 (M3320; Sigma), rabbit anti-MYF5 (ab125301; Abcam);

(11) Dynamic transcriptome changes during adipose tissue energy expenditure reveal critical roles for long noncoding RNA regulatorsPLOS BiologyJuly 3, 2017Zhiqiang Bai, Xiao-ran Chai, Myeong Jin Yoon, Hye-Jin Kim, Kinyui Alice LO, Zhi-chun Zhang, Dan Xu, Diana Teh Chee Siang, Arcinas Camille Esther Walet, Shao-hai Xu, Sook-Yoong Chia, Peng Chen, Hongyuan Yang, Sujoy Ghosh, Lei Sun

(12) CELF1 is a central node in post-transcriptional regulatory programmes underlying EMTNature communicationsNovember 21, 2016Chaudhury A, Cheema S, Fachini JM, Kongchan N, Lu G, Simon LM, Wang T, Mao S, Rosen DG, Ittmann MM, et al.subjected to hematoxylin and eosin staining and immunohistochemistry using anti-CUGBP1 antibody [3B1] (ab9549; Abcam, Cambridge, MA, USA) (1:500), DUSP2 (PA5-28775; Thermo Scientific, Rockford, IL, USA) (1:100),

(13) High-content screening identifies small molecules that remove nuclear foci, affect MBNL distribution and CELF1 protein levels via a PKC-independent pathway in myotonic dystrophy cell linesHuman molecular geneticsMarch 15, 2014Ami Ketley, Catherine Z Chen, Xin Li, Sukrat Arya, Thelma E Robinson, Javier Granados-Riveron, Inyang Udosen, Glenn E Morris, Ian Holt, Denis Furling, Soraya Chaouch, Ben Haworth, Noel Southall, Paul Shinn, Wei Zheng, Christopher P Austin, Christopher J Hayes, J David Brook

(14) Muscle weakness in myotonic dystrophy associated with misregulated splicing and altered gating of Ca(V)1.1 calcium channelHuman molecular geneticsMarch 15, 2012Tang ZZ, Yarotskyy V, Wei L, Sobczak K, Nakamori M, Eichinger K, Moxley RT, Dirksen RT, Thornton CA.

(15) CAG repeats mimic CUG repeats in the misregulation of alternative splicingNucleic Acids ResearchNovember 1, 2011Agnieszka Mykowska, Krzysztof Sobczak, Marzena Wojciechowska, Piotr Kozlowski, Wlodzimierz J. Krzyzosiak

(16) The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolismProceedings of the National Academy of Sciences of the United States of AmericaFebruary 2, 2010Clower CV, Chatterjee D, Wang Z, Cantley LC, Vander Heiden MG, Krainer AR.

(17) Temporal requirement of the alternative-splicing factor Sfrs1 for the survival of retinal neuronsDevelopment (Cambridge, England)December 1, 2008Rahul N Kanadia, Victoria E Clark, Claudio Punzo, Jeffrey M Trimarchi, Constance L Cepko

(18) Elevation of RNA-binding protein CUGBP1 is an early event in an inducible heart-specific mouse model of myotonic dystrophyThe Journal of clinical investigationOctober 1, 2007Guey-Shin Wang, Debra L Kearney, Mariella De Biasi, George Taffet, Thomas A Cooper

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Anti-CUG-BP1 antibody [3B1] from Abcam

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Anti-CUG-BP1 antibody [3B1] from Abcam

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