Anti-NFAT5 antibody ab3446 from Abcam

Product Specs
ItemAnti-NFAT5 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab3446
Antibody TypePrimary
Quantity50 UG
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
HostRabbit
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameNFAT5
ReactivityAfrican green monkey, Human, Mouse
TargetNFAT5

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) 2‐methoxyestradiol restrains non‐small cell lung cancer tumorigenesis through regulating circ_0010235/miR‐34a‐5p/NFAT5 axisThoracic CancerJuly 12, 2023Yong Zhang, Yuanyuan Mi, Chunhui Heblock tumor specimens. Tumor sections were then incubated with primary antibodies against NFAT5 (1:50, ab3446, Abcam), Ki67 (1:200, ab15580, Abcam) and MMP2 (1:500, ab97779) and secondary antibody (ab97051, 1:1000).

(2) A novel molecular mechanism mediated by circCCDC134 regulates non‐small cell lung cancer progressionThoracic CancerJuly 1, 2023Zhufeng Tong, Zhen Wang, Jinghan Jiang, Chengcheng Tong, Lan WuThen, primary antibodies (Abcam) against Bax (1:1000, ab32503), Bcl‐2 (1:1000, ab32124), NFAT5 (1:1000, ab3446), TSG101 (1:1000, ab30871), CD63 (1:1000, ab68418), and β‐actin (1:1000, ab8227) were incubated in the

(3) Exosomal MFI2-AS1 sponge miR-107 promotes non-small cell lung cancer progression through NFAT5Cancer cell internationalMarch 18, 2023Xu J, Wang H, Shi B, Li N, Xu G, Yan X, Xu L.primary antibodies: CD63 (ab134045, Abcam), Calnexin (ab133615, bacam), Tsg101 (ab125011, Abcam), NFAT5 (ab3446, Abcam), aAKT/p-AKT (CST, #4060, GAPDH (ab8245, Abcam). CCK8 We used Cell Counting Kit-8 (CCK8) to

(4) The nuclear factor of activated T cells 5 (NFAT5) contributes to the renal corticomedullary differences in gene expressionScientific ReportsNovember 24, 2022Dmitry Chernyakov, Annika Fischer, Max Brandau, Federica Petrillo, Robert A. Fenton, Bayram Edemirmicroscope (Keyence Corporation, Osaka, Japan). We used the following antibodies: anti-NFAT5 rabbit (ab3446, Abcam, Cambridge, UK) and Goat anti-Rabbit IgG (H + L) Alexa Fluor 488 (A-11034, Thermo Fisher Scentific). Conditional

(5) miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/Î²-Catenin PathwayStem Cells InternationalOctober 20, 2022Baodong Wang, Na Xu, Li Cao, Xiaojun Yu, Shanxi Wang, Qikun Liu, Yinguang Wang, Haoran Xu, Yang Caoa nitrocellulose membrane. Next, the membrane was incubated with primary antibodies against NFAT5 (ab3446, Abcam), β-catenin (ab6302, Abcam), cleaved caspase-3 (ab2302, Abcam), Bcl-2 (ab59348, Abcam), Bax (ab32503,

(6) MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5Computational and Mathematical Methods in MedicineJuly 5, 2022Fan Gong, Xiaoliang Li, Hanling Zhang, Jianke Wu, Guoxu Ma, Bowen Zhang, Jian Gao, Yi Ding, Yonglu Huang, Kun Xia, et al.(MMP)-1 (1 : 1000, Abcam, ab52631), anti-MMP-8 (1 : 1000, Abcam, ab81286), anti-NFAT5 (1 : 1000, Abcam, ab3446), antitumor necrosis factor-α (TNF-α) (1 : 1000, Abcam, MA5-23720), interleukin (IL)-6 (1 : 500, Abcam,

(7) Long noncoding RNA KCNQ1OT1 inhibits osteoclast differentiation by regulating the miR-128-3p/NFAT5 axisAging (Albany NY)May 31, 2022Hengshuo Zhang, Lu Chen, Ziyu Wang, Zhenqian Sun, Yu Shan, Qinghui Li, Linzeng Qi, Hongliang Wang, Yunzhen Chenand incubated with the following primary antibodies: rabbit polyclonal anti-NFAT5 (1:1000, Abcam, #ab3446, Cambridge, UK), rabbit monoclonal anti-NFATc1 (1:1000, CST, #8032), mouse monoclonal anti-c-Fos (1:1000,

(8) Bone marrow mesenchymal stem cell-derived exosomal microRNA-381-3p alleviates vascular calcification in chronic kidney disease by targeting NFAT5Cell Death & DiseaseMarch 28, 2022Yingjie Liu, Yan Guo, Shumin Bao, Hongdong Huang, Wenhu Liu, Weikang Guoperoxide and goat serum (ZSGB-Bio, ZLI-9022, Beijing, China), primary antibodies against NFAT5 (Abcam, ab3446, 1:100) were applied, followed by incubation with biotinylated secondary antibody. Dual-luciferase

(9) Circular RNA_0037128 aggravates high glucose-induced damage in HK-2 cells via regulation of microRNA-497-5p/nuclear factor of activated T cells 5 axisBioengineeredDecember 20, 2021Tao Feng, Weifang Li, Tianyi Li, Wenjun Jiao, Sufang Chen(TGF-β1; 1:1000, ab215715, Abcam), collagen type I (Col. I; 1:1000, ab34710, Abcam), NFAT5 (1:1000, ab3446, Abcam), β-actin (1:2000, ab8227, Abcam), as well as the secondary antibody (goat anti-rabbit; 1:10,000,

(10) NFAT5 Controls the Integrity of EpidermisFrontiers in ImmunologyDecember 9, 2021Khalid Muhammad et al.cytosolic and nuclear proteins on PAGE-SDS gels. For detecting NFAT5, the following Abs were used: Ab3446 (directed against the C-terminal aa1439-1455 of human NFAT5), Ab110995 (directed against an internal

(11) Loss of RANBP3L leads to transformation of renal epithelial cells towards a renal clear cell carcinoma like phenotypeJournal of Experimental & Clinical Cancer ResearchDecember 1, 2021Dmitry Chernyakov, Alexander GroÃŸ, Annika Fischer, Nicola Bornkessel, Christoph Schultheiss, Dennis Gerloff, Bayram Edemir100, 200, 400x or 1000x magnification. Antibodies We used following antibodies: anti-NFAT5 rabbit (ab3446, Abcam, Cambridge, UK), anti-GAPDH rabbit (14C10, Cell Signaling Technology, Danvers, Massachusetts,

(12) Circ_0001944 Contributes to Glycolysis and Tumor Growth by Upregulating NFAT5 Through Acting as a Decoy for miR-142-5p in Non-Small Cell Lung CancerCancer Management and ResearchMay 11, 2021Yawei Dou, Wei Tian, Hongtao Wang, Shanshan Lv1:1000, Abcam, Cambridge, MA, USA), LDHA (Lactate Dehydrogenase A) (ab125683, 1:1000, Abcam), NFAT5 (ab3446, 1:1000, Abcam), and β-actin (ab179467, 1:5000, Abcam). The membranes were incubated with a secondary

(13) Enterotoxigenic Bacteroides fragilis induces the stemness in colorectal cancer via upregulating histone demethylase JMJD2BGut MicrobesNovember 9, 2020Qian-Qian Liu, Chun-Min Li, Lin-Na Fu, Hao-Lian Wang, Juan Tan, Yun-Qian Wang, Dan-Feng Sun, Qin-Yan Gao, Ying-Xuan Chen, Jing-Yuan FangChina). Antibodies Primary antibodies used were anti-TLR4 (Abcam, Cambridge, MA, USA, ab13556), anti-NFAT5 (Abcam, ab3446), anti-JMJD2B (Bethyl Laboratories, Montgomery, TX, USA, A301-478), anti-NANOG (Cell Signaling Technology,

(14) Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissuesJOR SPINESeptember 1, 2020Abbie Binch, Joseph Snuggs, Christine L. Le MaitreIndian Hedgehog (Santa Cruz sc‐1196) staining, human NP tissue. C, Nuclear localisation of TonEBP (Abcam ab3446), human NP tissue. D, Pericellular and matrix staining of collagen type II (Abcam ab34712), human NP

(15) New Insights into the Pathogenesis of Intradialytic HypertensionThe Open Urology & Nephrology JournalDecember 31, 2018Joelle Hajal, Youakim Saliba, Najat Joubran, Ghassan Sleilaty, Dima Chacra, Shafika Assaad, Dania Chelala, Nassim Farèspolyclonal to VEGF-C; Abcam ab9546, Cambridge, UK), TonEBP [20] (1:1000; rabbit polyclonal to TonEBP; Abcam ab3446, Cambridge, UK), GAPDH [21] (Glyceraldehyde 3-phosphate dehydrogenase, reference protein) (1:2500; rabbit polyclonal

(16) Transcription Factor NFAT5 Promotes Glioblastoma Cell-driven Angiogenesis via SBF2-AS1/miR-338-3p-Mediated EGFL7 Expression ChangeFrontiers in molecular neuroscienceJanuary 1, 2017Yu H, Zheng J, Liu X, Xue Y, Shen S, Zhao L, Li Z, Liu Y.serial 4 μm sections and sections were labeled with primary antibodies against human NFAT5 (1:100; ab3446, Abcam, Cambridge, UK), followed by incubation with biotinylated secondary antibody included in an immunohistochemical

(17) NFAT5 participates in seawater inhalation-induced acute lung injury via modulation of NF-κB activityMolecular Medicine ReportsDecember 1, 2016Congcong Li, Manling Liu, Liyan Bo, Wei Liu, Qingqing Liu, Xiangjun Chen, Dunquan Xu, Zhichao Li, Faguang Jinaspiration-induced ALI. Materials and methods Drugs and reagents Monoclonal anti-NFAT5 (cat. no. ab3446) and monoclonal β-actin (cat. no. ab8226) antibodies were purchased from Abcam (Cambridge, UK). Anti-phosphorylated

(18) Functional Incompatibility between the Generic NF-κB Motif and a Subtype-Specific Sp1III Element Drives the Formation of the HIV-1 Subtype C Viral PromoterJournal of VirologyAugust 15, 2016Anjali Verma, Pavithra Rajagopalan, Rishikesh Lotke, Rebu Varghese, Deepak Selvam, Tapas K. Kundu, Udaykumar RangaUnited Kingdom), NFAT2 (catalog no. ab2796; Abcam, Cambridge, United Kingdom), and NFAT5 (catalog no. ab3446; Abcam, Cambridge, United Kingdom) were used. A nonspecific antibody raised against HIV-1 p24 (generated

(19) Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasisBreast cancer research : BCROctober 14, 2014Li JT, Wang LF, Zhao YL, Yang T, Li W, Zhao J, Yu F, Wang L, Meng YL, Liu NN, et al.

(20) Components of the Canonical and Non-Canonical Wnt Pathways Are Not Mis-Expressed in Pituitary TumorsPLoS ONEApril 26, 2013Leandro Machado Colli, Fabiano Saggioro, Luciano Neder Serafini, Renata Costa Camargo, Helio Rubens Machado, Ayrton Custodio Moreira, Sonir R. Antonini, Margaret de CastroSanta Cruz SC-7963), planar cell polarity (JNK - Abcam AB18680) and Calcium dependent (NFAT5 - Abcam AB3446) Wnt pathways. In ACTH-secreting pituitary tumors, IHC was performed in nine of the 18 samples studied

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Anti-NFAT5 antibody from Abcam

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