Anti-Collagen IV antibody ab6586 from Abcam

Product Specs
ItemAnti-Collagen IV antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab6586
Antibody TypePrimary
Quantity100 UG
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
FormatImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Concentration1 mg/ml
Gene NameCol4a1
ReactivityHuman
TargetCollagen IV

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal DefectsOrganogenesisDecember 31, 2023Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato, et al.anti-laminin (Abcam; 1:100; ab11575), anti-fibronectin (Abcam; 1:100; ab6328), and collagen IV (Abcam; 1:50; ab6586). Peroxidase-conjugated IgG polyclonal antibody was used as the secondary antibody (Histofine simple

(2) Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approachFrontiers in Cardiovascular MedicineNovember 29, 2023Kirill Yu. Klyshnikov, Maria A. Rezvova, Nikita V. Belikov, Tatiana V. Glushkova, Evgeny A. Ovcharenkoof bovine serum albumin for 1 h. Then, they were treated with antibodies against type IV collagen (ab6586, Abcam, Cambridge, UK) or antibodies against elastin (E4013, Sigma-Aldrich, St. Louis, MO, USA). Specimens

(3) 3D bioprinted multilayered cerebrovascular conduits to study cancer extravasation mechanism related with vascular geometryNature CommunicationsNovember 24, 2023Wonbin Park, Jae-Seong Lee, Ge Gao, Byoung Soo Kim, Dong-Woo ChoMA5-12023, Invitrogen, USA; Anti-TuJ1 antibody, 32-2600, Invitrogen, USA; Anti-Collagen IV antibody, ab6586, Abcam, UK; Anti-MAP2 antibody, Sigma-Aldrich, M4403; Anti-MAP2 antibody, Abcam, ab32454; Anti-ZO-1

(4) A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapyJournal of Tissue EngineeringNovember 17, 2023Michael J Buckenmeyer, Meena Sukhwani, Aimon Iftikhar, Alexis L Nolfi, Ziyu Xian, Srujan Dadi, Zachary W Case, Sarah R Steimer, Antonio D’Amore, Kyle E Orwig, et al.Acid-Schiff (PAS). Antibodies specific for ECM proteins Collagen I (Abcam, ab34710) and IV (Abcam, ab6586), Fibronectin (Abcam, ab23751), and Laminin (Abcam, ab11575) were evaluated using DAB (3,3′-Diaminobenzidine)

(5) Unlocking the potential of ultra-high dose fractionated radiation for effective treatment of glioblastomaResearch SquareNovember 13, 2023Xiao-Yan Lan, Lukasz Kalkowski, Cheng-Yan Chu, Anna Jablonska, Shen Li, Mihoko Kai, Yue Gao, Miroslaw Janowski, Piotr WalczakTechnology), Iba1 (1:250; Cat. 019-19741, Wako); CD45 (1:250; Cat. ab10558, Abcam), Collagen IV (1:300; Cat. ab6586, Abcam). Histochemical and immunofluorescent images were acquired with a DMi8 inverted microscope (Leica Microsystems

(6) Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cellsCell Death & DiseaseNovember 8, 2023Yakinthi Chrisochoidou, Rajat Roy, Pooyeh Farahmand, Guadalupe Gonzalez, Jennifer Doig, Lukas Krasny, Ella F. Rimmer, Anne E Willis, Marion MacFarlane, Paul H. Huang, et al.(#6763), MCP1 (CCL2) (#7201), IL-17A (#4887) were from ProSci. Antibodies against AR (ab45089), COL4A3 (ab6586), ENG (ab169545), FAP (ab207178), IL-1A (ab227482), IL-8 (ab18672) were from Abcam. Antibodies against

(7) Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networksNature communicationsSeptember 21, 2023Uçar MC, Hannezo E, Tiilikainen E, Liaqat I, Jakobsson E, Nurmi H, Vaahtomeri K.(1:300; R&D systems MAB2125), goat anti-VEGFR3 (1:150; R&D AF743), rabbit anti-collagen IV (1:150; Abcam Ab6586), chicken anti-GFP (1:1000; Abcam ab13970) and rabbit anti-RFP (1:500; Rockland 600-401-379). The used

(8) GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosisbioRxiv : the preprint server for biologySeptember 19, 2023Gobbo F, Zingariello M, Verachi P, Falchi M, Arciprete F, Martelli F, Peli A, Mazzarini M, Vierstra J, Mead-Harvey C, et al.Novus biologicals, Centennial, CO, USA), Collagen III (cat no. ab7778, Abcam) or Collagen IV (cat no. ab6586, Abcam) over night at 4°C. Cell binding of the primary antibodies was visualized with a secondary antibody

(9) MYCT1 attenuates renal fibrosis and tubular injury in diabetic kidney diseaseiScienceSeptember 15, 2023Xin Li, Qiu-Ling Fan, Tian-Kui Ma, Cong Liu, Hang Shi, Yuan-Yuan Sun, Yue Wang, Dong-Xue Ding, Ao Tang, Yu Qin, et al.RRID:AB_2082037" Rabbit polyclonal anti-Collagen IV (Western blot:1:2000; IHC:1:200),"Abcam, USA","Cat# ab6586, RRID:AB_305584" Rabbit polyclonal anti-Fibronectin (Western blot:1:2000; IHC:1:300),"Proteintech Co

(10) Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their ageFront Bioeng BiotechnolAugust 2, 2023Dominik Hanetseder, Tina Levstek, Andreas Herbert Teuschl-Woller, Julia Katharina Frank, Barbara Schaedl, Heinz Redl, Darja Marolt Presenincubated with primary antibodies against collagen type I (cat. No. ab34710), collagen type IV (cat. No. ab6586), laminin (cat. No. ab11575) and fibronectin (cat. No. ab2413) purchased from Abcam (Cambridge, UK)

(11) Pericyte dysfunction and impaired vasomotion are hallmarks of islets during the pathogenesis of type 1 diabetesCell ReportsAugust 1, 2023Luciana Mateus Mateus Gonçalves, Mirza Muhammad Fahd Fahd Qadir, Maria Boulina, Madina Makhmutova, Elizabeth Pereira, Joana AlmaçaA5228 CD31,Millipore,RRID: 550389 Tyrosine hydroxylase (TH),Millipore,RRID: AB152 Collagen IV,Abcam,RRID: AB6586 Periostin,R&D,RRID: AF2955 Insulin,Dako,RRID: IR002 Somatostatin,Millipore,RRID: MAB354 CD146,Abcam,RRID:

(12) HNRNPC promotes collagen fiber alignment and immune evasion in breast cancer via activation of the VIRMA-mediated TFAP2A/DDR1 axisMol MedAugust 1, 2023Bin Lian, Shuxun Yan, Jiayi Li, Zhengyang Bai, Jinping Ligoat serum is added. After incubating at room temperature for 5 min, rabbit anti-Collagen IV (1:500, ab6586, Abcam, USA) and rabbit anti-CD8 (1:2000, ab217344, Abcam, USA) primary antibodies are added and incubated

(13) A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantationScientific reportsJuly 24, 2023Kanai N, Inagaki A, Nakamura Y, Imura T, Mitsugashira H, Saito R, Miyagi S, Watanabe K, Kamei T, Unno M, et al.anti-insulin (Dako, Glostrup, Denmark), anti-collagen III (ab7778; Abcam, Cambridge, UK), anti-collagen IV (ab6586; Abcam) and anti-laminin (ab11575; Abcam) antibodies. EnVision+ System- HRP labelled polymer anti-rabbit

(14) Clopidogrel Administration Impairs Post-Stroke Learning and Memory Recovery in MiceInternational Journal of Molecular SciencesJuly 20, 2023Marina Paul, Jonathan W. Paul, Madeleine Hinwood, Rebecca J. Hood, Kristy Martin, Mahmoud Abdolhoseini, Sarah J. Johnson, Michael Pollack, Michael Nilsson, Frederick R. WalkerAustralia) for 30 min. Sections were then incubated in the presence of antibodies against Collagen IV (cat# ab6586, Abcam, Cambridge, UK; 1:1000), Iba1 (cat#019-19741, WAKO, Richmond, VA, USA; 1:1000), NeuN (cat#MAB377,

(15) Sigma-1 Receptor Activation Is Protective against TGFβ2-Induced Extracellular Matrix Changes in Human Trabecular Meshwork CellsLife (Basel)July 19, 2023Minh Ngoc Tran, Timea Medveczki, Balazs Besztercei, Gyorgy Torok, Attila J. Szabo, Xavier Gasull, Illes Kovacs, Andrea Fekete, Judit Hodrea(S1R, 1:1000, Santa Cruz sc137075; fibronectin, 1:5000, Abcam ab2413; collagen type IV, 1:500, Abcam ab6586; CTGF, 1:200, Santa Cruz sc14939; MMP2, 1:2000, Abcam ab92536) at 4 °C overnight. The membranes were

(16) Carbamoylated Erythropoietin-Induced Cerebral Blood Perfusion and Vascular Gene RegulationInternational Journal of Molecular SciencesJuly 15, 2023Jayanarayanan Sadanandan, Monica Sathyanesan, Yutong Liu, Neeraj K. Tiwari, Samuel S. Newton(Electron microscopy, Berkeley, CA, USA). Blood vessels were stained using collagen IV primary antibody (Abcam AB6586, Cambridge, UK) for 45 min at room temperature followed by Alexa fluor 488 secondary antibodies (Thermo

(17) A Novel Circ_Arf3/miR-452-5p/Mbnl1 Axis Regulates Proliferation and Expression of Fibrosis-Related Proteins of Mouse Mesangial Cells Under High GlucoseDiabetes, Metabolic Syndrome and ObesityJuly 11, 2023Qiong Wang, Yanting Zhu, Qianlan Dong, Linping Zhang, Wei ZhangI, 1:1000, ab34710, Abcam), anti-fibronectin (anti-FN, 1:1000, ab174833, Abcam), anti-Col IV (1:400, ab6586, Abcam), anti-GAPDH (1:2500, ab9485, Abcam), anti-Mbnl1 (1:1000, ab108519, Abcam), and goat Anti-Rabbit

(18) TIMP3 promotes the maintenance of neural stem-progenitor cells in the mouse subventricular zoneFrontiers in NeuroscienceJune 28, 2023Lingyan Fang, Takaaki Kuniya, Yujin Harada, Osamu Yasuda, Nobuyo Maeda, Yutaka Suzuki, Daichi Kawaguchi, Yukiko Gotohanti-Ascl1 (1:500, BD Biosciences Cat# 556604, RRID:AB_396479), anti-Collagen IV (1:50, Abcam Cat# ab6586, RRID:AB_305584), anti-Dcx (1:1000, Abcam Cat# ab18723, RRID:AB_732011), anti-EGFR (1:500, Fitzgerald Industries International

(19) Sustained in vivo perfusion of a re-endothelialized tissue engineered kidney graft in a human-scale animal modelFrontiers in Bioengineering and BiotechnologyJune 14, 2023Joseph S. Uzarski, Emily C. Beck, Emily E. Russell, Ethan J. Vanderslice, Matthew L. Holzner, Vikram Wadhera, Dylan Adamson, Ron Shapiro, Dominique S. Davidow, Jeff J. Ross, et al.human endothelial cells. Anti-Collagen I antibody (Abcam, #ab34710), Anti-Collagen IV antibody (Abcam, #ab6586), and Anti-Laminin antibody (Abcam, #ab11575) were used for matrix characterization. AlexaFluor 488

(20) Genetic Characterization of Rat Hepatic Stellate Cell Line PAV-1CellsJune 11, 2023Gäberlein K, Schröder SK, Nanda I, Steinlein C, Haaf T, Buhl EM, Sauvant P, Sapin V, Abergel A, Weiskirchen R.Netherlands",21–24,1:1000,r pAb Collagen I,NB600-408,"Novus Biologicals, Wiesbaden, Germany",139,1:1000,r mAb Collagen IV,ab6586,"Abcam, Berlin, Germany",161,1:1000,r pAb Desmin (D93F5),#5332,Cell Signaling,53,1:1000,r mAb Fibronectin,AB1954,Sigma-Aldrich,262,1:3000,r

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(1)

4/14/2022Western Blot Collagen 4 Marco PilliAbcam antibody works well for Western blot, but our results had several unspecific bands.
(1)

1/14/2020Good Antibody to Stain Collagen IV from Abcam Purnima GuptaVery good staining of collagen IV across the vasculature.
(1)

1/25/2017A Collagen IV Antibody That Works For Drosophila Ayla SessionsThe aim of our research is visualizing and quantifying the extracelullar matrix proteins in the Drosophila heart. Specifically we note how that change with age. There as of yet had been no anti-... Read Review

Anti-Collagen IV antibody from Abcam

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Anti-Collagen IV antibody from Abcam

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