Anti-AKT1 (phospho S473) antibody ab8932 from Abcam

Product Specs
ItemAnti-AKT1 (phospho S473) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab8932
Antibody TypePrimary
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityPolyclonal
Quantity100 UG
IsotypeIgG
Conjugate/TagUnconjugated
FormatThis product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum
Concentration1.02 mg/ml
ModificationsPhospho-S473
Gene NameAKT1
ReactivityHuman
TargetAKT1

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Vitexin suppresses the proliferation, angiogenesis and stemness of endometrial cancer through the PI3K/AKT pathwayPharmaceutical BiologyDecember 31, 2023Cuixia Liang, Yongjie Jiang, Lizhu Sunab21624), anti-p-PI3K (1:500; ab182651), anti-Total-PI3K (1:1000; ab133595), anti-p-AKT (S473) (1:500; ab8932), anti-p-AKT (T308) (1:500; ab38449), anti-Total-AKT (1:500; ab300473) and anti-β-actin (1:5000; ab8227)

(2) The Yunnan national medicine Maytenus compound inhibits the proliferation of hepatocellular carcinoma (HCC) by suppressing the activation of the EGFR-PI3K-AKT signaling pathwayJournal of CancerApril 7, 2021Wen-Tao Zhao et al.against EGFR (CST, #4405), p-EGFR (CST, #4407), PI3K (Abcam, ab189403), p-PI3K (Abcam, ab182651), AKT (Abcam, ab8932), p-AKT (Abcam, ab179463), FOXO3a (Abcam, ab109629), p-FOXO3a (Abcam, ab154786), p27 (Abcam, ab32034),

(3) Enforced expression of miR-92b blunts E. coli lipopolysaccharide-mediated inflammatory injury by activating the PI3K/AKT/β-catenin pathway via targeting PTENInternational Journal of Biological SciencesMarch 25, 2021Kangfeng Jiang, Jing Yang, Chunlian Song, Fengping He, Liangyu Yang, Xiaobing Libs-3332R, Bioss, China), rabbit anti-PI3K (1:1000, ab227204, Abcam, USA), rabbit anti-phospho-AKT (1:1000, ab8932, Abcam, USA), rabbit anti-AKT (1:500, GB111114, Servicebio, China), rabbit anti-β-catenin (1:5000, ab196204,

(4) Hsa_circ_0110757 upregulates ITGA1 to facilitate temozolomide resistance in glioma by suppressing hsa-miR-1298-5pCell Death & DiseaseMarch 5, 2021Haoyu Li, Qing Liu, Zihua Chen, Ming Wu, Chao Zhang, Jun Su, Yue Li, Chi ZhangH00003672, USA), PI3K primary antibody (Abcam, ab140307, USA), p-AKT (phospho S473) primary antibody (Abcam, ab8932, USA), and Bcl-2 primary antibody (Abcam, ab32124, USA) for overnight. After washing with PBS, an HRP-conjugated

(5) BHLHE40 modulates post-traumatic stress disorder behaviors with the involvement of the PI3K/AKT signaling pathwayAnais da Academia Brasileira de CiênciasJanuary 1, 2021ADILA AJI, RENA AIHEMAITI, SHAOHONG ZOU, ALIMUJIANG MAISIYITI, CHENG ZHANG, RUONAN LIU, XIAOKAITI SULIDAN93(3) e20201708 4 | 12 ADILA AJI et al. BHLHE40 ALLEVIATES PTSD-LIKE BEHAVIORS antibody (1:1000, ab8932), AKT antibody (1:1000, ab8805) and GAPDH antibody (1:1000, ab9485) at 4°C overnight, and then incubated

(6) Remodeling cancer stemness by collagen/fibronectin via the AKT and CDC42 signaling pathway crosstalk in gliomaTheranosticsJanuary 1, 2021Zhong C, Tao B, Tang F, Yang X, Peng T, You J, Xia K, Xia X, Chen L, Peng L.with primary anti-p-PI3KTyr607 antibody (ab182651, Abcam, Cambridge, UK) and anti-p-AKT1S473 antibody (ab8932, Abcam, Cambridge, UK) for overnight at 4 °C, followed by incubation with goat anti-rabbit Alexa Fluor®

(7) WITHDRAWN:Estrogen receptor β (ERβ) induces bovine ovarian granulosa cell (BGC) autophagy via the AKT/mTOR pathwayAsian-Australasian Journal of Animal SciencesDecember 1, 2020Yuxin Zheng, Ning Liu, Xiaorong Tang, Zhongliang Jiang# 188 ab101266, 1:2000, Abcam, UK), AKT (anti-AKT, # ab64148, 1:3000, Abcam, UK), 189 phospho-AKT (anti-pAKT, # ab8932, 1:3000, Abcam, UK), mTOR (anti-mTOR, 190 #ab2372, 1:2000, Abcom, UK), phospho-mTOR (anti-pmTOR, 1:2000,

(8) Electroacupuncture Protects Cognition by Regulating Tau Phosphorylation and Glucose Metabolism via the AKT/GSK3β Signaling Pathway in Alzheimer’s Disease Model MiceFrontiers in NeuroscienceNovember 20, 2020Anping Xu, Qingtao Zeng, Yinshan Tang, Xin Wang, Xiaochen Yuan, You Zhou, Zhigang Liantibody ab108387 Abcam, England Anti-pan-AKT antibody ab8805 Abcam, England Anti-AKT1 (phospho S473) antibody ab8932 Abcam, England Anti-GSK3 beta antibody ab93926 Abcam, England Anti-GSK3 beta (phospho S9) antibody ab131097 Abcam,

(9) Rho guanine nucleotide exchange factor 39 increases the viability, migration and invasion of clear cell renal cell carcinoma cells via the activation of the AKT/ERK signaling pathwayGenetics and Molecular BiologyNovember 18, 2020Shuzhong Wang, Yanmei Wang, Chuanyun WangARHGEF39 (1:1000, cat.no. ab67211, Abcam), AKT (1:500, cat. no. ab64148, Abcam), p-AKT (1:500, cat.no. ab8932, Abcam), ERK (1:1000, cat.no. ab32537, Abcam), p-ERK (1:1000, cat.no. ab131438, Abcam). Following that,

(10) MiR-185-3p regulates epithelial mesenchymal transition via PI3K/Akt signaling pathway by targeting cathepsin D in gastric cancer cellsTranslational Cancer ResearchNovember 1, 2020Cheng Huang, Yang-Jie Wu, Wei-Feng He, Shun-Li Zhao, Yan-Yi Ouyang, Xiao-Hong Ai, Zhi-Qi Liu, San-Yuan TangN-cadherin (ab18203), vimentin (ab92547), PI3K (ab86714), p-PI3K (ab182651), Akt (ab8805), p-Akt (Ser 473) (ab8932) and GAPDH (ab8245) were purchased from Abcam (Cambridge, MA, USA). Scratch assay For cell scratch

(11) Silencing of Peroxiredoxin 1 Inhibits the Proliferation of Esophageal Cancer Cells and Promotes Apoptosis by Inhibiting the Activity of the PI3K/AKT PathwayCancer Management and ResearchDecember 30, 2019Yingjian Song, Huimin Liu, Chunling Cui, Xiaonu Peng, Chaoyang Wang, Xudong Tian, Wenjun Li(1:500, orb137259; Biorbyt), anti-AKT (1:2000, ab235958; Abcam, Cambridge, UK), antip-AKT (1:1000, ab8932; Abcam), anti-PRDX1 (1:500, orb335510; Biorbyt), and polyclonal β-actin (1:1000, ab8227; Abcam). The

(12) miR-21-3p inhibits autophagy of bovine granulosa cells by targeting VEGFA via PI3K/AKT signalingReproductionNovember 1, 2019Lizhu Ma, Yuxin Zheng, Xiaorong Tang, Huimin Gao, Ning Liu, Yan Gao, Lizhuang Hao, Shujie Liu, Zhongliang Jiangpolyclonal to AKT, 1:3000, # ab64148, Abcam), phospho-AKT (Rabbit polyclonal to phosphor-AKT, 1:3000, #ab8932, Abcam) and β-actin (Rabbit polyclonal to β-actin, 1:5000; #4970, Cell Signaling Technology) in QuickBlock™ Primary

(13) MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTORPharmaceutical BiologySeptember 8, 2019Li Feng, Bo Xia, Bao-Fang Tian, Gong-Biao Ludiluted) were added for overnight reaction at 4 °C, including RICTOR (ab70374, Abcam), p-Akt (s473) (ab8932, Abcam), Akt (ab8805, Abcam), and GAPDH (ab181602, Abcam). The membrane with proteins on it was washed

(14) LncRNA SNHG6 inhibits cell proliferation and metastasis by targeting ETS1 via the PI3K/AKT/mTOR pathway in colorectal cancerMolecular Medicine ReportsJuly 19, 2019Su Meng, Zhao Jian, Xiaofei Yan, Jibin Li, Rui Zhang(1:2,000; ab26096), anti-p-PI3K (1:2,000; ab151549), anti- PI3K (1:2,000; ab32089) anti p-AKT (1:3,000; ab8932), anti AKT (1:2,000; ab38449), anti p-mTOR (1:2,000; ab109268), anti mTOR (1:2,000; ab2732) and anti-GAPDH

(15) lncRNA PLAC2 activated by H3K27 acetylation promotes cell proliferation and invasion via the activation of Wnt/β‑catenin pathway in oral squamous cell carcinomaInternational journal of oncologyApril 1, 2019Chen F, Qi S, Zhang X, Wu J, Yang X, Wang R.no. ab16663), anti-MMP-9 antibody (Abcam; cat. no. ab76003), anti-β-catenin antibody (Abcam; cat. no. ab8932) and anti-GAPDH antibody (Invitrogen; Thermo Fisher Scientific, Inc.; cat. no. PA1-987). Protein bands

(16) Activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast CancerMolecular cancerJanuary 8, 2019Dong H, Hu J, Zou K, Ye M, Chen Y, Wu C, Chen X, Han M.ab182651), anti-vimentin antibody (Abcam, cat. no. ab8805), anti-β-catenin antibody (Abcam, cat. no. ab8932), anti-GAPDH antibody (Invitrogen, cat. no. PA1–987). In vivo animal experiment Ten male BALB/c nude

(17) A Novel Aurora-A Inhibitor (MLN8237) Synergistically Enhances the Antitumor Activity of Sorafenib in Hepatocellular CarcinomaMolecular Therapy - Nucleic AcidsDecember 7, 2018Kai Zhang, Ting Wang, Hao Zhou, Bing Feng, Ying Chen, Yingru Zhi, Rui Wanganti-E-cadherin, ab15148; anti-N-cadherin, ab8203; anti-VEGFA, ab1316; anti-total Akt, ab8805; anti-p-Akt (S473), ab8932; anti-total p38-MAPK, ab27986; anti-p-p38-MAPK (Thr180/Tyr182), ab38238; and anti-GAPDH, ab8245] (Abcam)

(18) Clinical and genomic landscape of gastric cancer with a mesenchymal phenotypeNature CommunicationsDecember 1, 2018Sang Cheul Oh et al.directed against phospho-Akt1 (pS473) and phospho-ERK1/2 (pT202/pY204 for ERK1 and pT185/pY187 for ERK3) (ab8932 and ab50011, Abcam, Cambridge, UK) were diluted with phosphatebuffered saline in a ratio of 1:100. All

(19) Effect of long non-coding RNA Gas5 on proliferation, migration, invasion and apoptosis of colorectal cancer HT-29 cell lineCancer Cell InternationalDecember 1, 2018Jin Li, Yuan Wang, Cheng-Gong Zhang, Han-Juan Xiao, Hai-Juan Xiao, Jun-Ming Hu, Jun-Ming Hou, Jing-Dong He

(20) SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascadeCancer Cell InternationalDecember 1, 2018Shaoqiang Wang, Yingying Zheng, Zhiwei He, Wolong Zhou, Yuanda Cheng, Chunfang Zhanganti-SH2B1 (1:100, ab196575, Abcam), anti-Akt (1:500, ab8805, Abcam), anti-Akt (phospho S473) (1:200, ab8932, Abcam), anti-mTOR (1:1000, ab2732, Abcam), anti-mTOR (phosphor S2448) (1:50, ab109268, Abcam), anti-PTEN

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Anti-AKT1 (phospho S473) antibody from Abcam

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Anti-AKT1 (phospho S473) antibody from Abcam

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