Fig 1: Immunohistochemical staining of the paraffin-embedded solid form of EAT using proliferating cell nuclear antigen (PCNA) mouse antibody (Dako, M0879) at a dilution 1:100 (magnification 200×). Brown staining is visible in the nuclei of tumour cells. Highly proliferating tumour cells are located peripherally in the tumour mass, while the centre of the tumour is necrotic. Considering that all samples showed the same profile of very high proliferation (over 90%), we showed only one representative photomicrograph. Immunohistochemistry of the paraffin-embedded testis tissue stained using HIF-1a, Rabbit pAb (ABclonal, A16873) at a dilution 1:100 (magnification 100×) is presented in the middle, and immunohistochemistry of the paraffin-embedded breast cancer stained using iNOS, rabbit pAB at a dilution 1:120 (Abcam, ab3523) is presented on the right (magnification 100×). Representative photomicrographs of immunohistochemical slides were taken.