Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker ab6326 from Abcam

Product Specs
ItemAnti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab6326
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (PFA perfusion fixed frozen sections), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (PFA perfusion fixed frozen sections)
ClonalityMonoclonal
Quantity1 MG, 100 UG
CloneBU1/75 (ICR1)
HostRat
IsotypeIgG2a
Conjugate/TagUnconjugated
Concentration1 mg/ml
ReactivitySpecies independent
TargetBrdU

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Website: http://www.abcam.com

(1) Ethanol changes Nestin-promoter induced neural stem cells to disturb newborn dendritic spine remodeling in the hippocampus of miceNeural Regeneration ResearchFebruary 1, 2024Guixiang Wang, Wenjia Wang, Ye Zhang, Xiaoying Gou, Qingqing Zhang, Yanmiao Huang, Kuo Zhang, Haotian Zhang, Jingyu Yang, Yuting Lidoublecortin (DCX; rabbit, 1:1000, Abcam, Cat# Ab207175, RRID: AB_2894710), BrdU (rat, 1:500, Abcam, Cat# Ab6326, RRID: AB_305426), neuronal nuclei (NeuN; rabbit, 1:500, Merck, Cat# MAB377, RRID: AB_2298772), pS6

(2) FBXW7-loss sensitizes cells to ATR inhibition through induced mitotic catastropheCancer Research CommunicationsDecember 21, 2023Siobhan O'Brien, Tajinder Ubhi, Lucie Wolf, Krishna Gandhi, Sichun Lin, Naz Chaudary, Neesha C. Dhani, Michael Milosevic, Grant W. Brown, Stephane Angersimmunofluorescence analysis as described previously (17). CldU was detected using rat anti-BrdU (Abcam ab6326, 1:40), IdU was detected using mouse anti-BrdU clone B44 (BD Biosciences #347580, 1:10), and ssDNA was

(3) Targeting EIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancerJournal of Clinical InvestigationDecember 15, 2023Na Zhao, Elena B. Kabotyanski, Alexander B. Saltzman, Anna Malovannaya, Xueying Yuan, Lucas C. Reineke, Nadia Lieu, Yang Gao, Diego A. Pedroza, Sebastian J. Calderon, et al.Cell Signaling Technology [CST], 70076S), anti-S100A8 (1:5000; R&D Systems, MAB3059), anti-BrdU (1:1000; Abcam, ab6326), anti–cleaved caspase 3 (1:1000; CST, 9661), anti–phospho-histone H2A.X (Ser139) (1:400; CST, 9718),

(4) Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forksNucleic Acids ResearchDecember 11, 2023Yuqin Zhao, Kaiping Hou, Youhang Li, Shuailin Hao, Yu Liu, Yinan Na, Chao Li, Jian Cui, Xingzhi Xu, Xiaohua Wu, et al.BRCA2 (10741S, Cell Signaling Technology), MRE11 (ab208020, Abcam), RAD51 (ab133534, Abcam), BrdU (ab6326, Abcam and 347580, BD), MUS81(ab247136, Abcam), BLM (ab2179, Abcam), DNA2 (18727-1-AP, Proteintech),

(5) Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in agingElifeDecember 11, 2023Hirofumi Noguchi, Jessica Chelsea Arela, Thomas T. Ngo, Laura Cocas, Samuel J. Pleasurechicken anti-S100B (1:500, Synapyic Systems, 287 006, RRID:AB_2713986); rat anti-BrdU (1:500, Abcam, ab6326, RRID:AB_305426) and mouse anti-BrdU (1:100, BD Biosciences, 347580, RRID:AB_400326); rabbit anti-GluR2/3

(6) Permanent deconstruction of intracellular primary cilia in differentiating granule cell neuronsbioRxivDecember 8, 2023Carolyn M. Ott, Sandii Constable, Tri M. Nguyen, Kevin White, Wei-Chung Allen Lee, Jennifer Lippincott-Schwartz, Saikat Mukhopadhyayhumid chamber. Primary antibodies: ARL13B (1:1000, UC Davis/NeuroMab #75–287), BrdU (1:500, Abcam #ab6326), P27KIP (1:400, BD Biosciences) #610241), and SOX9 (1:500, Millipore #ABE571). For BrdU staining we

(7) Visualizing DNA replication by single-molecule analysis of replicated DNASTAR ProtocDecember 3, 2023Advaitha Madireddy, Jeannine Gerhardt1:15),Becton Dickinson,Cat #347580 Rat monoclonal anti-bromodeoxyuridine (dilution 1:15),Abcam,Cat #ab6326 "Goat anti-rat IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (dilution 1:15)",Invitrogen,Cat

(8) Characterization of BRCA2 R3052Q variant in mice supports its functional impact as a low-risk variantCell Death & DiseaseNovember 18, 2023Arun Prakash Mishra, Suzanne Hartford, Rajani Kant Chittela, Sounak Sahu, Suhas S. Kharat, Lucia Alvaro-Aranda, Aida Contreras-Perez, Teresa Sullivan, Betty K. Martin, Mary Albaugh, et al.min with 5% BSA in PBST. The fibers were then incubated with primary rat anti-BrdU antibody (Abcam ab6326, 1:500) and mouse anti-BrdU antibody (Becton Dickinson 347580, 1:500) for 2.5 h in the blocking solution.

(9) The splicing factor DHX38 enables retinal development through safeguarding genome integrityiScienceNovember 17, 2023Kui Sun, Yunqiao Han, Jingzhen Li, Shanshan Yu, Yuwen Huang, Yangjun Zhang, Jamas Reilly, Jiayi Tu, Pan Gao, Danna Jia, et al.antibodies: mouse anti-BrdU antibody (Becton Dickinson, Cat#347580) and rat anti-BrdU antibody (Abcam Cat#Ab6326), and then diluted in blocking solution and applied at 4°C overnight. Then, slides were washed twice

(10) Mouse primary follicles experience slow growth rates after activation and progressive increases that influence the duration of the primary follicle phase†Biology of ReproductionNovember 15, 2023Sharon Richard, Nicholas J Anderson, Yiran Zhou, Michael W PankhurstPBS, with 0.2% Tween 20 (Merck, cat# P1379) for 20 min. Primary antibody (rat anti-BrdU, Abcam, cat#: ab6326, RRID: AB_305426) was applied at 2 μg/mL PBS with 0.2% w/v Tween 20 (Sigma-Aldrich, Cat# P1379) and

(11) Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degenerationiScienceOctober 20, 2023Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou, et al.Na2B4O7 in water, PH8.5) for 15 min, prior to primary antibody incubation. An anti-BrdU antibody (1:250, ab6326, Abcam, UK) was incubated in blocking solution overnight at 4°C, and appropriate secondary antibody

(12) KDM2B regulates hippocampal morphogenesis by transcriptionally silencing Wnt signaling in neural progenitorsNature CommunicationsOctober 14, 2023Bo Zhang, Chen Zhao, Wenchen Shen, Wei Li, Yue Zheng, Xiangfei Kong, Junbao Wang, Xudong Wu, Tao Zeng, Ying Liu, et al.anti-CTIP2 (1:500; Abcam, ab18465), rabbit anti-SATB2 (1:500; Abcam, ab92446), rat anti-BrdU (1:500; Abcam, ab6326), mouse-anti-BrdU (1:500; Roche, 11170376001), rabbit-anti-DCX (1:500; Abcam, ab18723), rabbit anti-TBR2

(13) Regulation of young-adult neurogenesis and neuronal differentiation by neural cell adhesion molecule 2 (NCAM2)Cerebral CortexOctober 14, 2023Alba Ortega-Gascó, Antoni Parcerisas, Keiko Hino, Vicente Herranz-Pérez, Fausto Ulloa, Alba Elias-Tersa, Miquel Bosch, José Manuel García-Verdugo, Sergi Simó, Lluís Pujadas, et al.commercial primary antibodies were used for immunohistochemistry at the indicated dilution: anti-BrdU (ab6326, Abcam; 1:500); anti-ChFP (ab167453, Abcam, 1:300); anti-DCX (A8L1U, Cell Signaling, 1:500); anti-GFP

(14) P53 independent pathogenic mechanisms contribute to BubR1 microcephalyFrontiers in Cell and Developmental BiologyOctober 12, 2023Noelle A. Sterling, Bethany K. Terry, Julia M. McDonnell, Seonhee Kim#C10617). 2.4 Primary antibodies The following antibodies were used for immunohistochemistry: BrdU (ab6326, Abcam, 1:200), Cleaved Caspase-3 (#9661, Cell Signaling Technology, 1:200), CTIP2 (ab18465, Abcam,

(15) Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of strokeNature CommunicationsOctober 10, 2023Michael R. Williamson, Stephanie P. Le, Ronald L. Franzen, Nicole A. Donlan, Jill L. Rosow, Mathilda S. Nicot-Cartsonis, Alexis Cervantes, Benjamin Deneen, Andrew K. Dunn, Theresa A. Jones, et al.polyclonal anti-BrdU (1:500) Abcam ab152095, Rat monoclonal anti-BrdU [clone BU1/75 (ICR1)] (1:500) Abcam ab6326, Rabbit polyclonal anti-cleaved caspase 3 (1:500) Cell Signaling #9661, Mouse monoclonal anti-CXCL12

(16) Ribonucleotide synthesis by NME6 fuels mitochondrial gene expressionThe EMBO journalSeptember 18, 2023Grotehans N, McGarry L, Nolte H, Xavier V, Kroker M, Narbona-Pérez ÁJ, Deshwal S, Giavalisco P, Langer T, MacVicar T.NME6,Sigma,HPA017909 Human RCC1L,Proteintech,13796‐1‐AP Human TFAM,Proteintech,22586‐1‐AP DNA,Sigma,CBL186 BrdU,Abcam,ab6326 HRP‐conjugated secondary anti rabbit,BioRad,170‐6515 HRP‐conjugated secondary anti mouse,BioRad,170‐6516 Alexa

(17) Neuroimmune Support of Neuronal Regeneration and Neuroplasticity following Cerebral Ischemia in Juvenile MiceBrain SciencesSeptember 17, 2023Ricaurte A. Marquez-Ortiz, Vesna Tesic, Daniel R. Hernandez, Bilkis Akhter, Nibedita Aich, Porter M. Boudreaux, Garrett A. Clemons, Celeste Yin-Chieh Wu, Hung Wen Lin, Krista M. Rodgers(Vectashield, H-1000; Vector Laboratories). Primary antibodies included the following: rat anti-BrdU (ab6326; Abcam), rabbit anti-doublecortin (ab18723; Abcam), mouse anti-NeuN (MAB377; EMD Millipore), rabbit

(18) Alk1 acts in non-endothelial VE-cadherin(+) perineurial cells to maintain nerve branching during hair homeostasisNature communicationsSeptember 12, 2023Chovatiya G, Li KN, Li J, Ghuwalewala S, Tumbar T.AF1002); LYVE1 (1:400, Thermo Scientific, 14-0443-82); Prox1 (1:300, Abcam, ab199359); BrdU (1:300, Abcam, ab6326); Endomucin (1:300, Santa Cruz Biotechnology, sc-65495); CD34 (1:100, BD Biosciences, 553731); Laminin

(19) Conditional Deletion of Foxg1 Delayed Myelination during Early Postnatal Brain DevelopmentInternational Journal of Molecular SciencesSeptember 10, 2023Guangliang Cao, Congli Sun, Hualin Shen, Dewei Qu, Chuanlu Shen, Haiqin Luanti-MBP (Abcam, 1:1000, AB7349), rabbit anti-KI67 (Abcam, 1:500, AB16667), rat anti-BrdU (Abcam, 1:1000, AB6326, Cambridge, UK), mouse anti-SOX2 (Santa Cruz, 1:200, sc-365823, Dallas, TX, USA), mouse anti-CC1 (GeneTex,

(20) Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalonNeural DevelopmentSeptember 8, 2023Mary Jo Talley, Diana Nardini, Lisa A. Ehrman, Q. Richard Lu, Ronald R. Waclaw(1:1,000, Biogenesis, #2282), Rbt-βgal (1:1000, Biogenesis, #4600 − 1509), Rat-BrdU (1:250, Abcam, #AB6326), Rbt-CNPase (1:500, Cell Signaling, #5664), MS-CC1 (1:100, Calbiochem #OP80-100UG), GP-Gsx1 (1:5000,

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Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker from Abcam

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Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker from Abcam

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