Anti-YKL-40/CHI3L1 antibody ab77528 from Abcam

Product Specs
ItemAnti-YKL-40/CHI3L1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab77528
Conjugate/TagUnconjugated
ClonalityPolyclonal
HostRabbit
Antibody TypePrimary
Quantity100 UG
IsotypeIgG
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Concentration1 mg/ml
Gene NameCHI3L1
ReactivityHuman
TargetYKL-40 / CHI3L1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Proteomic analysis of radioiodine‑refractory differentiated thyroid cancer identifies CHI3L1 upregulation in association with dysfunction of the sodium‑iodine symporterOncology LettersDecember 7, 2022Yunjie Li, Fengqiong Hu, Jie Deng, Xin Huang, Chunyan Zhou, Mengxue Wu, Dong DuanZhongshan Golden Bridge Biotechnology Co., Ltd.). Antibodies targeting NIS (bs-0048R; Bioss) and CHI3L1 (ab77528; Abcam) were diluted to optimal concentrations (1:300 and 1:1,000, respectively). The primary antibodies

(2) Functional analysis of the short splicing variant encoded by CHI3L1/YKL-40 in glioblastomaFront OncolNovember 2, 2022Mengqi Shi, Qianyun Ge, Xinrong Wang, Wenbin Diao, Ben Yang, Sipeng Sun, Guohui Wang, Tian Liu, Andrew Man-Lok Chan, Zhiqin Gao, et al.resolved on an SDS–PAGE gel. The following antibodies were used for immunoblotting: YKL-40 (Abcam, #ab77528), YKL-40 (Cell Signaling, #47066), Actin (Santa Cruz, #sc-1615), anti-goat horseradish peroxidase (HRP)-conjugated

(3) A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migrationCell reportsOctober 18, 2022Ghochani Y, Muthukrishnan SD, Sohrabi A, Kawaguchi R, Condro MC, Bastola S, Gao F, Qin Y, Mottahedeh J, Iruela-Arispe ML, et al.RRID:AB_10861310 Anti-CD44 antibody,Cell Signaling,Cat#: 3570; RRID:AB_2076465 Anti-YKL40/CHI3L1 antibody,Abcam,Cat#: Ab77528; RRID:AB_2040911 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, pLV-FireflyLuc-GFP,UCLA

(4) Single-cell RNA sequencing of subcutaneous adipose tissues identifies therapeutic targets for cancer-associated lymphedemaCell discoveryJune 21, 2022Liu X, Yuan M, Xiang Q, Li Z, Xu F, Chen W, Chen J, Huang J, Yu N, Zhou Z, et al.The primary antibodies used for immunofluorescent staining included: PRG4 (ab28484, Abcam), CHI3L1 (ab77528, Abcam), CTHRC1 (ab85739, Abcam), CXCL14 (ab46010, Abcam), PDGFRα (MAB322, R&D), Cd11b (ab8878, Abcam),

(5) Anti‐Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarizationMolecular OncologyJune 1, 2022Ji Eun Yu, In Jun Yeo, Dong Ju Son, Jaesuk Yun, Sang‐Bae Han, Jin Tae HongLourmat, Collégien, France). The primary antibodies were as follows: anti‐Chi3L1 (dilution 1 : 1000; #ab77528, Abcam, Cambridge, UK), anti‐IL13Rα2 (dilution 1 : 1000; A2043, Abclonal, Wuhan, China), anti‐Cdk2 (dilution

(6) Role of Chitinase-3-like Protein 1 in Cardioprotection and Angiogenesis by Post-Infarction Exercise TrainingBiomedicinesApril 29, 2022Zhuo Li, Fangnan Wu, Lei Xi, Zhenjun Tianincubated with one of the following primary antibodies in the indicated concentrations: CHI3L1 (1:500, #ab77528, Abcam, Cambridge, MA, USA), PAR2 (1:500; #YN2681, Immunoway Biotechnology, Plano, TX, USA), PI3K (1:1000,

(7) SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent mannerMolecular therapy. Nucleic acidsMarch 8, 2022Zhang S, Zhao S, Qi Y, Li B, Wang H, Pan Z, Xue H, Jin C, Qiu W, Chen Z, et al.Cell Signaling Technology; 1:1,000), N-cadherin (13116; Cell Signaling Technology; 1:1,000), YKL40 (ab77528; Abcam; 1:1,000), CD44 (15675-1-AP; Proteintech; 1:1,000), MMP2 (40994; Cell Signaling Technology; 1:1,000),

(8) Single cell transcriptomic landscape of diabetic foot ulcersNature communicationsJanuary 10, 2022Theocharidis G, Thomas BE, Sarkar D, Mumme HL, Pilcher WJR, Dwivedi B, Sandoval-Schaefer T, Sîrbulescu RF, Kafanas A, Mezghani I, et al.anti-FAP (1:50, clone F11-24, sc-65398, Santa Cruz Biotechnology), rabbit polyclonal anti-CHI3L1 (1:100, ab77528, Abcam) and goat polyclonal anti-TIMP1 (1:100, AF970, R&D Systems). Alexa Fluor donkey anti-rabbit 488-,

(9) Chitinase-3 like-protein-1 promotes glioma progression via the NF-κB signaling pathway and tumor microenvironment reprogrammingTheranosticsJanuary 1, 2022Zhao T, Zeng J, Xu Y, Su Z, Chong Y, Ling T, Xu H, Shi H, Zhu M, Mo Q, et al.further performed for Hematoxylin and eosin (H&E) staining and IHC staining with anti-CHI3L1 (Cat. #ab77528, Abcam). For immunofluorescence (IF), frozen brain sections (6 μm) were incubated with anti-CHI3L1 (Cat.

(10) Chitinase 3-like 1 secreted from cancer-associated fibroblasts promotes tumor angiogenesis via interleukin-8 secretion in colorectal cancerInternational Journal of OncologyDecember 13, 2021Kaori Watanabe et al.CD90 (5E10; cat. no. 555593; BD Biosciences), α-SMA (1A4; cat. no. ab7817; Abcam) and CHI3L1 (cat. no. ab77528; Abcam) were used for immunostaining and immunofluorescence staining. Immunohistochemistry was performed

(11) Serum chitinase-3-like 1 protein is a useful biomarker to assess disease activity in ANCA-associated vasculitis: an observational studyArthritis Research & TherapyDecember 1, 2021Sung Soo Ahn, Taejun Yoon, Yong-Beom Park, Maria Prendecki, Gurjeet Bhangal, Stephen P. McAdoo, Sang-Won LeeFollowing heat-induced antigen retrieval, sections were incubated with anti-YKL-40 primary antibody (Ab77528, Abcam, Cambridge, UK) overnight at 4 °C, developed using an HRP-conjugated secondary system (Vector

(12) Activity of Combined Androgen Receptor Antagonism and Cell Cycle Inhibition in Androgen Receptor Positive Triple Negative Breast CancerMolecular Cancer TherapeuticsJune 1, 2021Jessica L. Christenson et al.antibody specific for GDF15 (#AF957, R&D Systems) and rabbit polyclonal antibody specific for CHI3L1 (#ab77528, Abcam). Tris-buffered saline with 0.05% Tween 20 was used for all washes. AR, FKBP5, PSA, and CHI3L1

(13) Databases for technical aspects of immunohistochemistry: 2021 updateJournal of toxicologic pathologyApril 1, 2021Moroki T, Matsuo S, Hatakeyama H, Hayashi S, Matsumoto I, Suzuki S, Kotera T, Kumagai K, Ozaki K.Fig. 35. CD163 /ED2 /Bio-Rad Laboratories, Inc. /MCA342R, Liver /Rat. Fig. 36. CHI3L1 /- /Abcam plc /ab77528, Liver /Mouse. Fig. 37. Chromogranin A /- /Nichirei Biosciences /412751, Adrenal gland / Dog. Fig. 38.

(14) Activation of ATF4 triggers trabecular meshwork cell dysfunction and apoptosis in POAGAging (Albany NY)March 31, 2021Ying Ying et al.markers, Matrix Gla Protein (MGP) (#ab86233, Abcam, Cambridge, MA, USA), Chitinase-3-Like-1 (CH3L1) (#ab77528, Abcam), Dexamethasone-induced cross-linked actin networks (CLANs) (#FAK100; Millipore, Billerica, MA,

(15) Steroid Hormone Receptor and Infiltrating Immune Cell Status Reveals Therapeutic Vulnerabilities of ESR1-Mutant Breast CancerCancer researchFebruary 1, 2021Williams MM, Spoelstra NS, Arnesen S, O'Neill KI, Christenson JL, Reese J, Torkko KC, Goodspeed A, Rosas E, Hanamura T, et al.RRID:AB_11172546), MUC1 (Cell Signaling Technology Cat# 4538, RRID:AB_2148549), and CHI3L1 (Abcam Cat# ab77528, RRID:AB_2040911). Representative images were taken at 200x, 400x, or 1000x magnification. Quantification

(16) Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cellsJournal of experimental & clinical cancer research : CRJanuary 7, 2021Chen Z, Wang H, Zhang Z, Xu J, Qi Y, Xue H, Gao Z, Zhao R, Wang S, Zhang S, et al.γ-H2AX (9718; Cell Signaling Technology; 1:1000), CD44 (3570; Cell Signaling Technology; 1:1000), YKL40 (ab77528; Abcam; 1:1000), SOX2 (3579; Cell Signaling Technology; 1:1000), Olig2 (ab109186; Abcam; 1:1000), phospho-NF-κB

(17) The oncogenic roles of 27‑hydroxycholesterol in glioblastomaOncology LettersJuly 31, 2019Lu Liu, Mei‑Yuan Li, Yu Xing, Xiao‑Yun Wang, Yong Wangab8805; 1:500 dilution, Abcam), anti-p70S6K (cat. no. ab32529; 1:500; Abcam), anti-YKL40 (cat. no. ab77528; 1:500; Abcam), anti-MAPK (cat. no. ab185145; 1:500; Abcam), anti-mTOR (cat. no. ab2732; 1:500; Abcam),

(18) CHI3L1 promotes tumor progression by activating TGF-β signaling pathway in hepatocellular carcinomaScientific reportsOctober 9, 2018Qiu QC, Wang L, Jin SS, Liu GF, Liu J, Ma L, Mao RF, Ma YY, Zhao N, Chen M, et al.protein-protein interactions. The cells were then incubated with the rabbit-antihuman CHI3L1 antibody ab77528 (Abcam, Shanghai, China) at 5 μg/ml overnight at 4 °C. The secondary antibody, goat anti-rabbit lgG

(19) TrkB-containing exosomes promote the transfer of glioblastoma aggressiveness to YKL-40-inactivated glioblastoma cellsOncotargetAugust 2, 2016Sandra Pinet, Barbara Bessette, Nicolas Vedrenne, Aurélie Lacroix, Laurence Richard, Marie-Odile Jauberteau, Serge Battu, Fabrice Lalloué1/1000) and β-actin (A 5316, 1/10000) were purchased from Sigma-Aldrich. Antibodies against YKL40 (ab77528, 1/1000), sortilin (ab16640, 1/1000), CD9 (ab92726, 1/1000) and HSP90 (ab13492, 1/1000), TSG101 (ab30871,

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Anti-YKL-40/CHI3L1 antibody from Abcam

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Anti-YKL-40/CHI3L1 antibody from Abcam

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