Anti-CLC-2 (CLCN2) Antibody ACL-002 from Alomone Labs, Ltd.

Product Specs
ItemAnti-CLC-2 (CLCN2) Antibody
CompanyAlomone Labs, Ltd.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberACL-002
Brand/TrademarkAlomone Labs Ltd
HostRabbit
Quantity1 X 50 mcl, 1 X 0.2 ml
Conjugate/TagUnconjugated
ReactivityHuman (Tested);Mouse (Tested);Rat (Tested)
ApplicationsICC, IF, IHC, WB
FormatLyophilized Powder, Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3
ClonalityPolyclonal
Antibody TypePrimary
Concentration06 mg/ml
PurityAffinity purified on immobilized antigen
IsotypeIgG
ImmunogenSynthetic peptide
Domain/Region/TerminusIntracellular, C-terminus
Research AreaEpilepsy, Neuroscience, Taste, Gustatory System, Visual, Auditory, Auditory System, Hearing, Sensory System, Cancer, tumor, tumour, Cardiovascular, Hypertension, Pulmonary Hypertension, Vascular Hypertension, Neurological Diseases, Depression
Gene NameCLCN2
TargetCLC-2 (CLCN2) Channel
ValidationAs per applications, See images, contact for clarifications
pH RangepH 7.4

Specifications/Features

starting price for North American orders, for exact pricing view product page

Sequence

RSRHGLPREGTPSDSDDKC, corresponding to amino acid residues 888-906 of rat CLC-2

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Alomone Labs, Ltd.

PO Box 4287

Jerusalem 91042

Israel

Phone: +972-2-587-2202
+972-2-587-1101

Company Profile

Website: http://www.alomone.com/

(1) Olfactory neuronal cells as a promising tool to realize the “druggable genome” approach for drug discovery in neuropsychiatric disordersFrontiers in NeuroscienceMarch 10, 2023Marina Mihaljevic, Max Lam, Carlos Ayala-Grosso, Finn Davis-Batt, David J. Schretlen, Koko Ishizuka, Kun Yang, Akira Sawabuffered saline with 0.1% Tween 20. Membranes were incubated with an antibody against CLCN2 (Alomone Labs, ACL-002) (1:200 dilution) or with an antibody against GAPDH (Santa Cruz Biotech, sc-32233) (1:4000 dilution)

(2) Parallel intermediate conductance K(+) and Cl(-) channel activity mediates electroneutral K(+) exit across basolateral membranes in rat distal colonAmerican journal of physiology. Gastrointestinal and liver physiologyAugust 1, 2020Rehman S, Narayanan K, Nickerson AJ, Coon SD, Hoque KM, Sandle GI, Rajendran VM.

(3) Molecular identification of HSPA8 as an accessory protein of a hyperpolarization-activated chloride channel from rat pulmonary vein cardiomyocytesThe Journal of biological chemistryNovember 1, 2019Okamoto Y, Nagasawa Y, Obara Y, Ishii K, Takagi D, Ono K.

(4) Knockout of ClC-2 reveals critical functions of adherens junctions in colonic homeostasis and tumorigenicityAmerican journal of physiology. Gastrointestinal and liver physiologyDecember 1, 2018Jin Y, Ibrahim D, Magness ST, Blikslager AT.transfer to a polyvinylidene difluoride membrane, the membranes were probed using anti-ClC-2 (1:200, ACL-002; Alomone Laboratories, Jerusalem, Israel) and anti␤-actin (1:20,000, ab8227; Abcam) antibodies. Horseradish

(5) CLCN2 chloride channel mutations in familial hyperaldosteronism type IINature geneticsMarch 1, 2018Scholl UI, Stölting G, Schewe J, Thiel A, Tan H, Nelson-Williams C, Vichot AA, Jin SC, Loring E, Untiet V, et al.extracted from stable cell lines, and inducible expression was verified by western blot (ClC-2 antibody #ACL-002, Alomone labs, Jerusalem, Israel). The HAC15 cell line was kindly provided by Dr. William Rainey (University

(6) Identification of the fatty acid activation site on human ClC-2American Journal of Physiology-Cell PhysiologyJune 1, 2017John Cuppoletti, Kirti P. Tewari, Jayati Chakrabarti, Danuta H. MalinowskaRestore Western Blot Stripping Buffer from Thermo Fisher Scientific (Rockford, IL). Rabbit anti-ClC-2 antibody (no. ACl-002) was purchased from Alomone Laboratories (Jerusalem, Israel); horseradish peroxidase (HRP)conjugated

(7) Severe changes in colon epithelium in the Mecp2-null mouse model of Rett syndromeMolecular and cellular pediatricsDecember 1, 2016Millar-Büchner P, Philp AR, Gutierrez N, Villanueva S, Kerr B, Flores CA.Briefly, 4 mM paraffin sections were incubated with the corresponding primary antibody: ClC-2 1:400 (ACL-002, Alomone), NHE-3 1:1000 (C-20, Santa Cruz), and NKCC1 1:4000 (kindly provided by R. James Turner, [10]).

(8) Characterization of Human Dermal Fibroblasts in Fabry DiseaseJournal of cellular physiologyJanuary 1, 2016Lakomá J, Donadio V, Liguori R, Caprini M.

(9) Genetic Ablation of the ClC-2 Cl- Channel Disrupts Mouse Gastric Parietal Cell Acid SecretionPloS oneJanuary 1, 2015Nighot MP, Nighot PK, Ma TY, Malinowska DH, Shull GE, Cuppoletti J, Blikslager AT.

(10) Increase in hypotonic stress-induced endocytic activity in macrophages via ClC-3Molecules and cellsMay 1, 2014Yan Y, Ding Y, Ming B, Du W, Kong X, Tian L, Zheng F, Fang M, Tan Z, Gong F.

(11) Chloride channel ClC-2 is a key factor in the development of DSS-induced murine colitisInflammatory bowel diseasesDecember 1, 2013Nighot P, Young K, Nighot M, Rawat M, Sung EJ, Maharshak N, Plevy SE, Ma T, Blikslager A.

(12) Dual activation of CFTR and CLCN2 by lubiprostone in murine nasal epitheliaAmerican journal of physiology. Lung cellular and molecular physiologyMarch 1, 2013Schiffhauer ES, Vij N, Kovbasnjuk O, Kang PW, Walker D, Lee S, Zeitlin PL.

(13) Severe defects in absorptive ion transport in distal colons of mice that lack ClC-2 channelsGastroenterologyFebruary 1, 2012Marcelo A Catalán, Carlos A Flores, Mireya González-Begne, Yan Zhang, Francisco V Sepúlveda, James E Melvin

(14) ClC-2 contributes to tonic inhibition mediated by α5 subunit-containing GABA(A) receptor in experimental temporal lobe epilepsyNeuroscienceJuly 14, 2011Ge YX, Liu Y, Tang HY, Liu XG, Wang X.

(15) Lubiprostone activates Cl- secretion via cAMP signaling and increases membrane CFTR in the human colon carcinoma cell line, T84Digestive diseases and sciencesFebruary 1, 2011Ao M, Venkatasubramanian J, Boonkaewwan C, Ganesan N, Syed A, Benya RV, Rao MC.

(16) Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colonThe Journal of membrane biologyMay 1, 2010Inagaki A, Yamaguchi S, Takahashi-Iwanaga H, Iwanaga T, Ishikawa T.

(17) Functional role of CLC-2 chloride inward rectifier channels in cardiac sinoatrial nodal pacemaker cellsJournal of molecular and cellular cardiologyJuly 1, 2009Huang ZM, Prasad C, Britton FC, Ye LL, Hatton WJ, Duan D.

(18) Clcn2 encodes the hyperpolarization-activated chloride channel in the ducts of mouse salivary glandsAmerican journal of physiology. Gastrointestinal and liver physiologyNovember 1, 2008Romanenko VG, Nakamoto T, Catalán MA, Gonzalez-Begne M, Schwartz GJ, Jaramillo Y, Sepúlveda FV, Figueroa CD, Melvin JE.

(19) Properties and possible function of a hyperpolarisation-activated chloride current in DrosophilaThe Journal of experimental biologyJuly 1, 2007Rose U, Derst C, Wanischeck M, Marinc C, Walther C.

(20) Membrane cholesterol content modulates ClC-2 gating and sensitivity to oxidative stressThe Journal of biological chemistryJanuary 26, 2007Hinzpeter A, Fritsch J, Borot F, Trudel S, Vieu DL, Brouillard F, Baudouin-Legros M, Clain J, Edelman A, Ollero M.

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