Anti-Ninein/NIN antibody - Centrosome Marker ab4447 from Abcam

Product Specs
ItemAnti-Ninein/NIN antibody - Centrosome Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab4447
Antibody TypePrimary
Quantity100 UG
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence
IsotypeIgG
ClonalityPolyclonal
Conjugate/TagUnconjugated
Gene Namenin
ReactivityHuman
TargetNinein

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacementiScienceJune 17, 2022Fdez E, Madero-Pérez J, Lara Ordóñez AJ, Naaldijk Y, Fasiczka R, Aiastui A, Ruiz-Martínez J, López de Munain A, Cowley SA, Wade-Martins R, et al.rabbit polyclonal anti-cNAP1 (Proteintech, 14498-1-AP, 1:500), rabbit polyclonal anti-ninein (Abcam, ab4447, 1:500), rabbit polyclonal anti-cenexin (Abcam, ab43840, 1:500) and mouse monoclonal anti-cyclinB1 (Santa Cruz Biotechnology

(2) Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary ciliumPLoS biologyJune 1, 2020Aydin ÖZ, Taflan SO, Gurkaslar C, Firat-Karalar EN.San Diego, CA), rabbit Arl13B (Neuromab, Clone N295B/66, Davis, CA) at 1:500, and anti-Ninein (Abcam, ab4447, Cambridge, MA) at 1:500. Rabbit anti-PCM1, anti-Cep120, and anti-GFP antibodies were generated and

(3) Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16PLoS geneticsSeptember 1, 2018Shi CH, Rubel C, Soss SE, Sanchez-Hodge R, Zhang S, Madrigal SC, Ravi S, McDonough H, Page RC, Chazin WJ, et al.incubated overnight at 4 °C with 10 μg of anti-AMPKα1 (R and D Systems, AF3197), anti-CHIP (Abcam, ab4447), rabbit IgG or goat IgG antibodies. 120 μl Protein-G Dynabeads (Invitrogen) was then added to each

(4) Centrobin controls primary ciliogenesis in vertebratesThe Journal of cell biologyApril 2, 2018Ogungbenro YA, Tena TC, Gaboriau D, Lalor P, Dockery P, Philipp M, Morrison CG.CEP135 (Bird and Hyman, 2008; 1420 738; 1:1,000); CPAP (1:1,000; 11517-1-AP; ProteinTech); Ninein (Ab4447; 1:250; Abcam); SDCCAG8 (1:250; 13471-1-AP; ProteinTech); γ-tubulin (1:1,000; T3559; Sigma-Aldrich);

(5) Spindle pole cohesion requires glycosylation-mediated localization of NuMAScientific ReportsDecember 1, 2017Jérémy Magescas et al.anti-γ-tubulin (T3559; 1:200) antibodies were from Sigma-Aldrich (St Louis, Missouri, USA). Rabbit anti-ninein (ab4447; 1:100), rabbit anti-NuMA (ab109262; 1:200) and mouse anti-O-GlcNAc (ab2739; 1/500) antibodies were

(6) Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplificationMolecular biology of the cellMarch 15, 2017Flanagan AM, Stavenschi E, Basavaraju S, Gaboriau D, Hoey DA, Morrison CG.pericentrin (1:2000; ab4448; Abcam), detyrosinated tubulin (1:500; ab48389; Abcam), ninein (1:200; ab4447; Abcam), Arl13b (1:500; 17711-1-AP; Proteintech), rootletin (1:750; NBP1-80820; Novus), PCM1 (1:10,000;

(7) A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodiesMolecular Biology of the CellJanuary 1, 2017Li-En Jao, Abdalla Akef, Susan R. Wentetarget region, amino acids 414–656), rabbit anti-PCNT (1:500; ab4448; Abcam), rabbit anti-NIN (1:500, ab4447; Abcam), mouse anti–γ-tubulin (1:300; GTU-88; Sigma-Aldrich), rat anti–α-tubulin (1:500; YOL1/34; Accurate

(8) Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic catReproduction in domestic animals = ZuchthygieneNovember 3, 2016T Rowlison, M A Ottinger, P Comizzoli

(9) 14-3-3γ Prevents Centrosome Amplification and Neoplastic ProgressionScientific reportsJune 2, 2016Mukhopadhyay A, Sehgal L, Bose A, Gulvady A, Senapati P, Thorat R, Basu S, Bhatt K, Hosing AS, Balyan R, et al.1:200), α-tubulin (Abcam ab7291; dilution 1:500), centrin1 (Abcam ab11257; dilution 1:50), Ninein (Abcam ab4447; dilution 1:50), 14-3-3γ (CG31; Abcam ab76525; dilution 1:200) antibodies were used for immunofluorescence.

(10) CEP164-null cells generated by genome editing show a ciliation defect with intact DNA repair capacityJournal of cell scienceMay 1, 2016Owen M Daly, David Gaboriau, Kadin Karakaya, Sinéad King, Tiago J Dantas, Pierce Lalor, Peter Dockery, Alwin Krämer, Ciaran G Morrison

(11) Centrosome-intrinsic mechanisms modulate centrosome integrity during feverMolecular biology of the cellOctober 1, 2015Vertii A, Zimmerman W, Ivshina M, Doxsey S.prepared as described (Zheng et al., 1995). The following commercial antibodies were used: ninein (ab4447; Abcam [Cambridge, MA]), pericentrin (ab4448; Abcam), cenexin/ODF2 (ab43840; Abcam), centrobin (ab70448;

(12) Centrin2 regulates CP110 removal in primary cilium formationThe Journal of cell biologyMarch 16, 2015Prosser SL, Morrison CG.ab75005; Abcam), Pericentrin (1:1,000; ab4448; Abcam), Centrin2 (1:500; 6288; BioLegends), Ninein (1:200; ab4447; Abcam), Cep164 (1:250; NBP1-77006; Novus Biologicals), Cep170 (1:1,000; raised against His-tagged amino

(13) LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein complexHuman molecular geneticsJanuary 15, 2014Hyang Mi Moon, Yong Ha Youn, Hayley Pemble, Jessica Yingling, Torsten Wittmann, Anthony Wynshaw-Boris

(14) The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanismsBiology openDecember 15, 2013Jodoin JN, Shboul M, Albrecht TR, Lee E, Wagner EJ, Reversade B, Lee LA.CETN2 (N-17-R, 1:200, Santa Cruz Biotechnology, Dallas, TX), PCTN (ab4448, 1:2000, Abcam), Ninein (ab4447, 1:500, Abcam), dynein intermediate chain (74.1, 1:500, Abcam), and PH3 (Mitosis Marker, 1:1000, Millipore,

(15) Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate cellsMolecular Biology of the CellSeptember 1, 2013Burcu Inanç, Monika Pütz, Pierce Lalor, Peter Dockery, Ryoko Kuriyama, Fanni Gergely, Ciaran G. Morrison1:200, PCM1 (817; a gift from A. Merdes, University of Toulouse, Toulouse, France) at 1:5000, ninein (ab4447; Abcam) at 1:100, protein G (Abcam) at 1:1000, centrin3 (Abnova, San Jose, CA) at 1:1000, and TACC3

(16) The PTEN-Akt pathway impacts the integrity and composition of mitotic centrosomesCell cycle (Georgetown, Tex.)May 1, 2013Mary K Leonard, Natasha T Hill, Paula A Bubulya, Madhavi P Kadakia

(17) Promoter hijack reveals pericentrin functions in mitosis and the DNA damage responseCell cycle (Georgetown, Tex.)February 15, 2013Yifan Wang, Tiago J Dantas, Pierce Lalor, Peter Dockery, Ciaran G Morrison

(18) Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repairPloS oneJanuary 1, 2013Dantas TJ, Daly OM, Conroy PC, Tomas M, Wang Y, Lalor P, Dockery P, Ferrando-May E, Morrison CG.at 1:500; Centrin3 (3E6, Abnova) at 1:1000; mouse anti-myc 9E10 at 1:500 (for DT40 cells); Ninein (ab4447, Abcam) at 1:200; PCM-1 (817, a gift from Andreas Merdes [60]) at 1:10000; Pericentrin (ab4448, Abcam)

(19) STIL is required for centriole duplication in human cellsJournal of Cell ScienceMarch 1, 2012Julia Vulprecht et al.

(20) The expression and localization of the human placental prorenin/renin-angiotensin system throughout pregnancy: roles in trophoblast invasion and angiogenesis?PlacentaDecember 1, 2011K G Pringle, M A Tadros, R J Callister, E R Lumbers

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Anti-Ninein/NIN antibody - Centrosome Marker from Abcam

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Anti-Ninein/NIN antibody - Centrosome Marker from Abcam

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