Anti-Histone H2A.Z antibody - ChIP Grade ab4174 from Abcam

Product Specs
ItemAnti-Histone H2A.Z antibody - ChIP Grade
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab4174
Antibody TypePrimary
HostRabbit
Quantity100 UG
IsotypeIgG
ApplicationsChIP, Immunocytochemistry/ Immunofluorescence, Western blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameH2AFZ
ReactivityCow, Human, Mouse, Rat
TargetHistone H2A.Z

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) A complex interplay between H2A.Z and HP1 isoforms regulates pericentric heterochromatinFrontiers in Cell and Developmental BiologyNovember 9, 2023Jessica González, Laia Bosch-Presegué, Anna Marazuela-Duque, Anna Guitart-Solanes, María Espinosa-Alcantud, Agustín F. Fernandez, Jeremy P. Brown, Juan Ausió, Berta N. Vazquez, Prim B. Singh, et al.were used for each ChIP and were incubated ON with the following antibodies: 7.5 µg α-H2A.Z (Abcam, ab4174), 7.5 µg α-H2A (Cell Signaling, D603A) and 7.5 µg α-HA (Sigma-Aldrich, H6908), corresponding rabbit IgG

(2) Retrospective analysis of enhancer activity and transcriptome historyNature biotechnologyNovember 1, 2023Boers R, Boers J, Tan B, van Leeuwen ME, Wassenaar E, Sanchez EG, Sleddens E, Tenhagen Y, Mulugeta E, Laven J, et al.and nuclei. Samples were incubated O/N with 1:100 primary antibody (rabbit anti-histone H2A.Z, Abcam, ab4174; rabbit anti-acetyl histone H2A.Z, Merck, ABE1363; or rabbit anti-H3K27me3, Cell Signaling Technology,

(3) DDM1-mediated gene body DNA methylation is associated with inducible activation of defense-related genes in ArabidopsisGenome BiologyMay 5, 2023Seungchul Lee, Jaemyung Choi, Jihwan Park, Chang Pyo Hong, Daeseok Choi, Soeun Han, Kyuha Choi, Tae-Young Roh, Daehee Hwang, Ildoo HwangChIP sequencing library, the sonicated chromatin was immunoprecipitated with 5 μg of anti-H2A.Z (Abcam, ab4174) or anti-H1 (Agrisera, AS11 1801) antibody and with 40 μl of Dynabeads Protein G (Invitrogen, 10003D).

(4) The impact of SWI/SNF and NuRD inactivation on gene expression is tightly coupled with levels of RNA polymerase II occupancy at promotersGenome ResearchMarch 16, 2023Sachin Pundhir, Jinyu Su, Marta Tapia, Anne Meldgaard Hansen, James Seymour Haile, Klaus Hansen, Bo Torben Porsefragmented chromatin in IP buffer was immunoprecipitated using either 5 µg of antihistone H2A.Z antibody (ab4174, Abcam) or recombinant antihistone H3.3 antibody (Abcam ab176840). Each ChIP was spiked (2%) with sonicated

(5) Chromatin remodeler Znhit1 controls bone morphogenetic protein signaling in embryonic lung tissue branchingThe Journal of biological chemistryOctober 1, 2022Wei W, Tang X, Jiang N, Ni C, He H, Sun S, Yu M, Yu C, Qiu M, Yan D, et al.and washed with PBS. ChIP protocol was done as previously described (12). The anti-H2A.Z antibody (ab4174; Abcam) and protein A/G magnetic beads (Millipore) were used for ChIP. ChIP–qPCR was performed with

(6) Hierarchical Accumulation of Histone Variant H2A.Z Regulates Transcriptional States and Histone Modifications in Early Mammalian EmbryosAdvanced ScienceAugust 1, 2022Xin Liu et al.preincubating 11 µL Dynabeads Protein G (10003D, Invitrogen) with 2 µg antibody of H2A.Z (ab188314, Abcam; ab4174, Abcam; ab150402, Abcam; 07–594, Millipore), H3K4me3 (ab8580, Abcam), H3K27me3 (07–449, Millipore) and

(7) Mitotically heritable, RNA polymerase II-independent H3K4 dimethylation stimulates INO1 transcriptional memoryeLifeMay 17, 2022Bethany Sump, Donna G Brickner, Agustina D'Urso, Seo Hyun Kim, Jason H BricknerIgG, from Thermo Fisher Scientific; α -H3K4me2 (ab32356), α-GFP (ab290), α-Myc (ab32), and α-H2A.Z (ab4174) from Abcam; α-RNA Polymerase II (cat:664906) from BioLegend; α-TAP (Product #CAB1001) from Invitrogen.

(8) Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathyJCI insightMarch 22, 2022Shi Y, Fan W, Xu M, Lin X, Zhao W, Yang Z.5′-TTCGCTACCTTCGACAGCAAG-3′ and 5′-GATGGTCATAGGCTCTTCCATG-3′. Antibodies. Znhit1 (ab238125), H2A.Z (ab4174), and H3K4me3 (ab1012) were purchased from Abcam (Cambridge); H3K27me3 (9733) was from Cell Signaling Technology

(9) HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCsNucleic acids researchJanuary 11, 2022Yang Y, Zhang L, Xiong C, Chen J, Wang L, Wen Z, Yu J, Chen P, Xu Y, Jin J, et al.Applied Biosystems StepOnePlus system. Antibodies used for ChIP were as follows: H2A.Z antibody (Abcam, ab4174), SRCAP antibody (Kerafast ESL103) and spike-in antibody (Active Motif, 61686). The primers used for

(10) Chromatin states shaped by an epigenetic code confer regenerative potential to the mouse liverNature CommunicationsDecember 1, 2021Chi Zhang, Filippo Macchi, Elena Magnani, Kirsten C. Sadler1 µg/µl; used 6 µl), anti-H3K9me3 (Active Motif, 39161; 1 µg/µl; used 6 µl), and anti-H2AZ (Abcam, ab4174; 1 µg/µl; used 10 µl). The prepared libraries were sequenced on the Hiseq2500 platform for 75 bp single-end

(11) Cleavage-embryo genes and transposable elements are regulated by histone variant H2A.XJournal of Reproduction and DevelopmentOctober 29, 2021Kai-yi SUN, Shi-meng GUO, Gui-ping CHENG, Ying YIN, Ximiao HE, Li-quan ZHOUHoechst. The primary antibodies used included anti-H2A.X (ab11175, Abcam, Cambridge, UK) and anti-H2A.Z (ab4174, Abcam), anti-macroH2A (61428, active motif, Carlsbad, CA, USA), and anti-H2A (D210-3, MBL, Nagoya,

(12) Linking nuclear matrix–localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.ZJournal of Biological ChemistryOctober 1, 2021Zhaosu Chen et al.H2A (Abcam, ab18255), histone H2B (Abcam, ab1790), histone H4 (Abcam, ab10158), histone H2A.Z (Abcam, ab4174), Lamin A/C (Abcam, ab133256), PIAS1 (Abcam, ab109388), SAE1 (Abcam, ab185552), SAE2 (Abcam, ab185955),

(13) OsARP6 Is Involved in Internode Elongation by Regulating Cell-Cycle-Related GenesBiomoleculesJuly 26, 2021Aziz Ul Ikram, Yong Ding, Yanhua Sufor 10 min and then quenched with 0.125 M glycine. Then, 5 µg of the specific antibodies anti-H2A.Z (ab4174, lot: GR292900-1, Abcam, Cambridge, UK), or control IgG serum, were added to the pre-cleared supernatants

(14) Stress-induced transcriptional memory accelerates promoter-proximal pause release and decelerates termination over mitotic divisionsMolecular CellApril 1, 2021Anniina Vihervaara, Dig Bijay Mahat, Samu V. Himanen, Malin A.H. Blom, John T. Lis, Lea Sistonenover night at 4°C using the following ChIP-verified antibodies: HSF1 (Spa-901, Enzo), H2.AZ (Abcam, ab4174), H3 (Merck Millipore, 06–755), H4 (Merck Millipore, 05–858) and AcH4 (06–866, Upstate). Proteins were

(15) Decoding the protein composition of whole nucleosomes with Nuc-MSNature MethodsMarch 1, 2021Luis F. Schachner et al.Information. Antibodies used: H2A.Z (Cell Signaling Technologies, CST, #2718S) Lot #2; H2A.Z (Abcam, #ab4174) Lot# GR3198864-2; H3.3 (EMD/Millipore, #09-838) Lot #3310680; H4K16ac (CST, #13534S) Lot #3; H3K79me2

(16) Disrupting the DREAM transcriptional repressor complex induces apolipoprotein overexpression and systemic amyloidosis in miceJournal of Clinical InvestigationFebruary 15, 2021Pirunthan Perampalam, Haider M. Hassan, Grace E. Lilly, Daniel T. Passos, Joseph Torchia, Patti K. Kiser, Andrea Bozovic, Vathany Kulasingam, Frederick A. Dickμg, MilliporeSigma, anti–B-MYB mouse antibody MABE886; H2AZ: 5 μg, Abcam, anti–histone H2A.Z rabbit antibody ab4174), and then combined with lysed and sonicated samples and incubated overnight at 4°C with rotation. Dynabeads

(17) Epigenetic priming by Dppa2 and 4 in pluripotency facilitates multi-lineage commitmentNature Structural & Molecular BiologyAugust 1, 2020Mélanie A. Eckersley-Maslin, Aled Parry, Marloes Blotenburg, Christel Krueger, Yoko Ito, Valar Nila Roamio Franklin, Masashi Narita, Clive S. D’Santos, Wolf ReikFor each ChIP, 5μg antibody (anti-Ash2l Bethyl Labs A300-489, anti-Ezh2 CST D2C9, anti-H2A.Z Abcam ab4174, anti-H3K4me3 Abcam ab8580, anti-H3K27me3 Active Motif AM39155, anti-IgG Abcam ab125938, anti-Ring1b

(18) Histone variant H2A.Z regulates nucleosome unwrapping and CTCF binding in mouse ES cellsNucleic Acids ResearchJune 19, 2020Zengqi Wen, Liwei Zhang, Haihe Ruan, Guohong Liparticle size for ChIP-seq. For H2A.Z, H2A or H3 ChIP, chromatin was first incubated with H2A.Z (Abcam, ab4174), H2A (Abcam, ab18255) or H3 (Abcam, ab1791) antibody in RIPE-150 (50 mM Tris–HCl, 150 mM NaCl, 1 mM

(19) Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulationeLifeFebruary 28, 2020Lamaa A, Humbert J, Aguirrebengoa M, Cheng X, Nicolas E, Côté J, Trouche D.were prepared and analysed by standard Western blotting protocol, using antibodies against total H2AZ (ab4174, Abcam), GAPDH (clone 6C5, MAB374, Millipore), PHF14 (SAB3500960, Sigma and proteintech 24787–1-AP),

(20) The Evening Complex Establishes Repressive Chromatin Domains Via H2A.Z DepositionPlant PhysiologyJanuary 1, 2020Meixuezi Tong et al.Millipore), anti-Pol II (cat. no. sc-33754; Santa Cruz Biotechnology), anti-H2A.Z antibodies (cat. no. ab4174; Abcam), and salmon sperm DNA/protein A agarose beads (cat. no. 16-157; Millipore) were used for ChIP. DNA

Write a Review

(1)

9/11/2018Good Anti-Histone H2A.Z AntibodyDr. Raghavendra VadlaOur goal was to study the stability of H2A in the nucleosomes. For that, we immunoprecipitated Flag-H2A nucleosomes from yeast cell lysates and washed the beads with different NaCl concentration ... Read Review

Anti-Histone H2A.Z antibody - ChIP Grade from Abcam

Abcam

Citations (126)

Reviews

Anti-Histone H2A.Z antibody - ChIP Grade from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map