Anti-EAAT1 antibody ab416 from Abcam

Product Specs
ItemAnti-EAAT1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab416
Antibody TypePrimary
ClonalityPolyclonal
IsotypeIgG
Quantity100 UG
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Conjugate/TagUnconjugated
Gene NameSLC1A3
ReactivityHuman, Rat
TargetEAAT1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Chronic exposure to inorganic arsenic and fluoride induces redox imbalance, inhibits the transsulfuration pathway, and alters glutamate receptor expression in the brain, resulting in memory impairment in adult male mouse offspringArchives of ToxicologyJuly 23, 2023Wendy L. González-Alfonso, Petrosyan Pavel, Hernández-Mercado Karina, Luz M. Del Razo, Luz C. Sanchez-Peña, Angélica Zepeda, María E. GonsebattUSA). For Western blots, primary rabbit antibodies against xCT (ab37185), EAAT3 (ab124802), GLAST (ab416), GLT1 (ab41621), NR2B (ab65783), GluA2 (ab133477), Synaptophysin (SY38, ab8049), CBS (ab135626), and

(2) Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewaliScienceJuly 21, 2023Rieskamp JD, Rosado-Burgos I, Christofi JE, Ansar E, Einstein D, Walters AE, Valentini V, Bruno JP, Kirby ED.anti-DCX (1:500 IF),Cell Signaling,Cat#4604; RRID:AB_561007 Rabbit anti-EAAT1 (1:250 IF),Abcam,Cat#ab416; RRID:AB_304334 Guinea Pig anti-EAAT2 (1:1000 IF),Sigma,Cat#ab1783; RRID:AB_90949 Rabbit anti-GFAP

(3) Astrocytic Neuroligins Are Not Required for Synapse Formation or a Normal Astrocyte CytoarchitecturebioRxivJuly 13, 2023Samantha R. Golf, Justin H. Trotter, George Nakahara, Thomas C. SüdhofRRID:AB_2572352",1:5000 Antibody,Anti-Kir4.1,Millipore Sigma,"AB5818, RRID:AB_92053",1:1000 Antibody,Anti-EEAT1,Abcam,"ab416, RRID:AB_304334",1:1000 Antibody,Anti-MAP2,EnCOR Biotechnology,"CPCA-MAP2, RRID:AB_2138173",1:500 Antibody,Anti-Gephyrin,Synaptic Systems

(4) Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemiaBMC Complementary Medicine and TherapiesMay 11, 2023Rafael Short Ferreira, Paulo Roberto Ribeiro, Juliana Helena Castro e Silva, Juliana Bender Hoppe, Monique Marylin Alves de Almeida, Beatriz Correia de Lima Ferreira, Gustavo Borges Andrade, Suzana Braga de Souza, Luzimar Gonzaga Ferdandez, Maria de Fátima Dias Costa, et al.(1:5000, Merck Millipore Chemicon International, ab1783), rabbit anti-GLAST antibody (1: 150, Abcam, ab416), or rabbit anti-GS (1:5000, Abcam, ab49873). Following washes in PBS-T, slices were incubated in appropriate

(5) Spinal cord tissue engineering using human primary neural progenitor cells and astrocytesBioengineering & translational medicineMarch 1, 2023Jin C, Wu Y, Zhang H, Xu B, Liu W, Ji C, Li P, Chen Z, Chen B, Li J, et al.ratio Nestin,NEUROMICS,MO15012,Mouse,1:200 SOX2,GeneTex,GTX101507,Rabbit,1:500 PAX3,Abcam,ab180754,Rabbit,1:200 Ki67,Abcam,ab15580,Rabbit,1:500 Tuj‐1,GeneTex,GTX631836,Mouse,1:500 Tuj‐1,Abcam,ab18207,Rabbit,1:500 DCX,Abcam,ab113435,Goat, MAP2,Sigma‐Aldrich,M4403,Mouse,1:250 MAP2,Abcam,ab32454,Rabbit,1:250 NF70,Sigma‐Aldrich,N4142,Rabbit,1:200 NeuN,Abcam,ab177487,Rabbit,1:500 GFAP,Abcam,ab7260,Rabbit,1:500 GFAP,Abcam,ab134436,chicken,1500 OLIG2,Millipore,AB9610,Rabbit,1:500 S100β,Abcam,ab52642,Rabbit,1:500 GLAST,Abcam,ab416,Rabbit,1:500 S100A4,Biolegened,"810,101",Rabbit,1:200 SOX‐9,R&D Systems,AF3075,Goat,1:200 P2Y12,GeneTex,GTX54796,Rabbit,1:1000 Iba1,Abcam,ab15690,Mouse,1:500 GFP,Abcam,ab290,Rabbit,1:500 GFP,GeneTex,GTX13970,Chicken,1:500 vGLUT1,Millipore,AB5905,Guinea Pig

(6) Adolescent intermittent ethanol exposure reduces astrocyte-synaptic proximity in the adult medial prefrontal cortex in rats: Reversal by gabapentinAddiction NeuroscienceJanuary 16, 2023Kati L. Healey, Amelia Bell, Michael D. Scofield, H.S. Swartzwelder[Guinea Pig anti-GLT1, 1:5000 (cat#AB1783, Millipore Sigma, St, Louis, MO) and Rabbit anti-EAAT1, 1:3000 (cat#ab416, Abcam, Cambridge, MA)] and followed the next day with IRDye secondary antibodies diluted to 1:15,000

(7) Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammalseLifeNovember 29, 2022Iyer AA, Hosamani I, Nguyen JD, Cai T, Singh S, McGovern MM, Beyer L, Zhang H, Jen HI, Yousaf R, et al.17-5791-80,AB_2734965,1:300 POU4F3,Rabbit polyclonal,Proteintech 21509–1-AP,AB_2878872,1:200 GLAST,Rabbit polyclonal,Abcam ab416,AB_304334,1:300 SOX2,Rat monoclonal,Biocompare 14-9811-80,AB_11219070,1:250 SOX9,Rabbit polyclonal,Millipore Sigma

(8) Inhibition of Synaptic Glutamate Exocytosis and Prevention of Glutamate Neurotoxicity by Eupatilin from Artemisia argyi in the Rat CortexInternational journal of molecular sciencesNovember 2, 2022Lu CW, Wu CC, Chiu KM, Lee MY, Lin TY, Wang SJ.and β-actin (#3700) were acquired from Cell Signaling (Beverly, MA, USA). Antibodies against EAAT1 (ab416) and EAAT2 (ab41621) were acquired from Abcam (Cambridge, UK). Horseradish peroxidase-conjugated secondary

(9) Electroacupuncture relieves hyperalgesia by regulating neuronalâ€“glial interaction and glutamate transporters of spinal dorsal horns in rats with acute incisional neck painFrontiers in NeuroscienceOctober 26, 2022Jun-ying Wang, Jin-ling Zhang, Shu-ping Chen, Yong-hui Gao, Jian-liang Zhang, Yu Chen, Yue Zhang, Pei-jing Rong, Jun-ling Liuanti-Iba-1 (1:500, ab5076, Abcam, Waltham, MA, USA), or mouse anti-GFAP and rabbit anti-GLAST (1:200, ab416, Abcam, Waltham, MA, USA)/rabbit anti-GLT-1 (1:200, ab41621, Abcam, Waltham, MA, USA) overnight at 4°C.

(10) Glyphosateâ€based herbicide induces longâ€lasting impairment in neuronal and glial differentiationEnvironmental ToxicologyAugust 1, 2022LuÃ£ Reis, Marilena Raciti, Patricia GonzÃ¡lez Rodriguez, Bertrand Joseph, Ibrahim Al Rayyes, Per UhlÃ©n, Anna Falk, Suzana Telles da Cunha Lima, Sandra Ceccatelli#12202),1:500 ICQ,NESTIN (Millipore MAB5326),1:500 NESTIN (Millipore MAB5326),WB,1:1000 ICQ,GLAST (Abcam ab416),1:500 ICQ,GLT‐1 (Millipore AB1783),1:500 ICQ,"NICD (Abcam, ab8925)",1:500 ICQ,MAP2 (Sigma‐Aldrich

(11) Disruption of Glutamate Homeostasis in the Brain of Rat Offspring Induced by Prenatal and Early Postnatal Exposure to Maternal High-Sugar DietNutrientsMay 24, 2022Jozef Mizera, Bartosz Pomierny, Anna Sadakierska-Chudy, Beata Bystrowska, Lucyna Pomierny-Chamioloantibodies,Western blot—primary antibodies,Western blot—primary antibodies EAAT1,1:1000,"Abcam (Cambridge, UK)",ab416 EAAT2,1:500,"Abcam (Cambridge, UK)",ab178401 xCT,1:1000,"Abcam (Cambridge, UK)",ab175186 VGLUT1,1:1000,"Abcam

(12) Derivation and Characterization of Murine and Amphibian Müller Glia Cell LinesTranslational vision science & technologyApril 1, 2022Gallo RA, Qureshi F, Strong TA, Lang SH, Pino KA, Dvoriantchikova G, Pelaez D.MA1-813,Mouse,1:1000WB,AB_2178528 GFAP,Abcam Cat# ab10062,Mouse,1:1000IF,AB_296804 GLAST (EAAT1),Abcam Cat# ab416,Rabbit,1:200IF,AB_304334 ,,,1:1000WB, GS,Millipore Cat# MAB302,Mouse,1:200IF,AB_2110656 ,,,1:1000WB, Pax6,BioLegend

(13) Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterizationSTAR protocolsMarch 18, 2022Anastasaki C, Wilson AF, Chen AS, Wegscheid ML, Gutmann DH.Anti-GBX2 (1:50),Lifespan Biosciences,Cat# LS-C197281 Rabbit Polyclonal Anti-EAAT1 (1:500),Abcam,Cat# ab416; RRID: AB_304334 Mouse Monoclonal Anti-GFAP (1:500),Abcam,Cat# ab4648; RRID: AB_449329 Mouse Monoclonal

(14) Reactive Astrocytes Contribute to Alzheimer’s Disease-Related Neurotoxicity and Synaptotoxicity in a Neuron-Astrocyte Co-culture AssayFrontiers in Cellular NeuroscienceJanuary 21, 2022David Wasilewski, Nelson David Villalba-Moreno, Inke Stange, Markus Glatzel, Diego Sepulveda-Falla, Susanne Krasemann(1:1,000) cat# MAB360, (clone GA5 Millipore, Burlington, MA, United States), anti-GLAST (1:1,000) cat# ab416, Abcam, Cambridge, United Kingdom. Neuronal and synaptic marker: anti-synaptophysin antibody (1:250),

(15) Fluoxetine increases astrocytic glucose uptake and glycolysis in corticosterone-induced depression through restricting GR-TXNIP-GLUT1 PathwayFrontiers in pharmacologyJanuary 1, 2022Pan SM, Zhou YF, Zuo N, Jiao RQ, Kong LD, Pan Y.fixed by 1% PFA. The fixed cells were incubated with primary antibodies rabbit anti-GLAST1 (1:100, ab416, Abcam) and mouse anti-GLUT1 (1:100, ab40084, Abcam) in permeabilized (0.1% Triton, for total GLUT1

(16) Human iPSC-derived astrocytes generated from donors with globoid cell leukodystrophy display phenotypes associated with diseasePloS oneJanuary 1, 2022Lieberman R, Cortes LK, Gao G, Park H, Wang B, Jones PL, Hunter RB, Leonard JP, Barker RH Jr.Abcam Ab52642), mouse anti-vimentin (1:1000, Sigma-Aldrich V2258), and mouse anti-GLAST (1:100, Abcam Ab416). Primary antibodies were incubated overnight at 4˚C, following which cells were washed four times in

(17) Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brainJournal of extracellular vesiclesJanuary 1, 2022You Y, Muraoka S, Jedrychowski MP, Hu J, McQuade AK, Young-Pearse T, Aslebagh R, Shaffer SA, Gygi SP, Blurton-Jones M, et al.MA3915), anti‐L1 cell adhesion molecule (L1CAM, 1:200, eBioscience, 14‐1719‐82), anti‐EAAT1 (1:250, Abcam, ab416), anti‐ low‐density lipoprotein receptor‐related protein 1 (LRP1, 1:250, Thermo Fisher Scientific, 37–7600),

(18) Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined mediaSTAR ProtocolsDecember 17, 2021Sylvain Perriot, Mathieu Canales, Amandine Mathias, Renaud Du Pasquier1:200),Sigma-Aldrich,Cat#AB5804 anti-S100β rabbit IgG (dilution 1:200),Abcam,Cat#AB52642 anti-GLAST rabbit IgG (dilution 1:200),Abcam,Cat#AB416 anti-rabbit goat IgG AF488 (dilution 1:200),Thermo Fisher Scientific,Cat#A-21206 anti-rabbit donkey

(19) Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brainJournal of neuroscience researchDecember 1, 2021Muñoz Y, Cuevas-Pacheco F, Quesseveur G, Murai KK.produced in rabbit,"Synthetic peptide corresponding to Rat EAAT1 (C terminal, 20 residues)","Abcam, # Ab416, polyclonal antibody, RRID:AB_304334",2 μg/ml/1:500 Anti‐Kir4.1 antibody produced in guinea pig,Peptide

(20) Disruption of Glutamate Release and Uptake-Related Protein Expression After Noise-Induced Synaptopathy in the CochleaFrontiers in Cell and Developmental BiologyAugust 4, 2021Kefeng Ma, Anran Zhang, Xiaojun She, Honglian Yang, Kun Wang, Yingwen Zhu, Xiujie Gao, Bo Cuiat room temperature. Rabbit anti-CtBP2 (1:100; Bioworld, BS2287), rabbit anti-GLAST (1:1,000, Abcam, Ab416), and mouse anti-Na+/K+-ATPase α1 (1:500, Millipore, #05-369) were applied overnight or for 2 h at room

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11/27/2018Rabbit Polyclonal to EAAT1 Karthik KrishnamurthyIsolated primary astrocytes were firstly tested for purity using the glutamate transporter GLAST as a marker of mature astrocytes. The antibody is specific and works well for the detection of EAAT1.

Anti-EAAT1 antibody from Abcam

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Anti-EAAT1 antibody from Abcam

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