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Antibodies
Anti-KAT13D / CLOCK antibody
Anti-KAT13D / CLOCK antibody from Abcam
(1)
Write a review
Citations:
(42)
Supplier Page
Supplier Page from
Abcam for
Anti-KAT13D / CLOCK antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-KAT13D / CLOCK antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab3517
Antibody Type
Primary
Clonality
Polyclonal
Applications
Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Isotype
IgG
Quantity
200 UL
Host
Rabbit
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
CLOCK
Reactivity
Human, Mouse
Target
KAT13D / CLOCK
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (42)
(1)
A druggable copper-signalling pathway that drives inflammation
Nature
May 1, 2023
Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD,
et al
.
Ms), CLOCK (
Proteintech
,
18094-1-AP
, WB
1:1,000
, Hu, KD/KO validated by manufacturer), CLOCK (
Abcam
,
ab3517
, WB
1:1,000
, Ms),
CTR1
(
Abcam
,
ab129067
,
clone
EPR7936, lot GR3414582-4 and GR81444-2, WB
1:1,000
, Hu,
(2)
Identification of Novel Targets for Treatment of Dilated Cardiomyopathy Based on the Ferroptosis and Immune Heterogeneity
Journal of inflammation research
January 1, 2023
Lu H, Xie Y, Zhou Z, Hong P, Chen J.
(bs-17563R,
1:200
, Bioss, China),
CD19
(
14–0194-82
,
1:100
,
ThermoFisher
, USA),
4-hydroxynonenal
(4-HNE) (
ab3517
,
Abcam
, Cambridge, UK). The secondary antibodies used are as follows: Goat Anti-Mouse IgG H&L (TRITC)
(3)
Co-alterations of circadian clock gene transcripts in human placenta in preeclampsia
Scientific reports
October 25, 2022
Zhou G, Winn E, Nguyen D, Kasten EP, Petroff MG, Hoffmann HM.
blocked in 3% milk for 1 h at RT and incubated with rabbit anti-
human
CLOCK primary antibody (
1:1000
,
ab3517
,
Abcam
, Cambridge, UK, RRID: AB_303886) for 16 h at 4 °C. After washing, the blot was incubated in
goat anti-rabbit IgG-HRP
(4)
Chronic sleep deprivation altered the expression of circadian clock genes and aggravated Alzheimer''s disease neuropathology
Brain pathology (Zurich, Switzerland)
May 1, 2022
Niu L, Zhang F, Xu X, Yang Y, Li S, Liu H, Le W.
Cambridge, UK),
anti‐Cryptochrome I/CRY1
(
ab104736
,
1:200
,
Abcam
, Cambridge, UK),
anti‐KAT13D/CLOCK
(
ab3517
,
1:500
,
Abcam
, Cambridge, UK), phospho‐Tau (Thr231) monoclonal antibody (AT180) (
MN1040
,
1:100
,
Thermo Fisher Scientific
(5)
miR-92b-3p Exerts Neuroprotective Effects on Ischemia/Reperfusion-Induced Cerebral Injury via Targeting NOX4 in a Rat Model
Oxidative Medicine and Cellular Longevity
March 30, 2022
Yongpan Huang, Jiayu Tang, Xiaojuan Li, Xian Long, Yansong Huang, Xi Zhang
MA, USA), sealed with 5% skim milk powder, and incubated with the anti-NOX4 primary antibody (cat.
ab3517
:
Abcam
, Cambridge, UK) overnight. Then, the protein was incubated again with the HRP-coupled secondary
(6)
Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration
International journal of molecular sciences
September 30, 2021
Cha S, Lee SM, Wang J, Zhao Q, Bai D.
instruction. The following antibodies were used: Bmal1 (
1:250
, #NB100-2288, Novusbio), Clock (
1:100
, #
ab3517
,
Abcam
), GFP (
1:100
, #NB600-597, Novusbio),
Acan
(
1:200
, #
13880-1-AP
,
Proteintech
), Collagen II (
1:100
,
(7)
L-3,4-Dihydroxyphenylalanine Recovers Circadian Rhythm Disturbances in the Rat Models of Parkinson's Disease by Regulating the D1R-ERK1/2-mTOR Pathway
Frontiers in Aging Neuroscience
August 19, 2021
Shuyuan Yang, Ying Wan, Na Wu, Lu Song, Zhihua Liu, Jiahao Zhao, Ying Liu, Zhenguo Liu, Jing Gan
tyrosine hydroxylase (TH;
1:1,000
,
ab41528
,
Abcam
, UK), BMAL1 (
1:1,000
,
ab3350
,
Abcam
), CLOCK (
1:1,000
,
ab3517
,
Abcam
), ERK1/2 (
1:1,000
,
4695S
,
Cell Signaling
, USA), p-
ERK
(
1:1,000
,
4370S
,
Cell Signaling
, USA),
(8)
Decreased expression of the clock gene Bmal1 is involved in the pathogenesis of temporal lobe epilepsy
Molecular brain
July 14, 2021
Wu H, Liu Y, Liu L, Meng Q, Du C, Li K, Dong S, Zhang Y, Li H, Zhang H.
were used: anti-Bmal1 (
Abcam
,
ab93806
), anti-β-actin (
Proteintech
,
66009-1-Ig
), anti-CLOCK (
Abcam
,
ab3517
), anti-
Per2
(
Abcam
,
ab179813
), anti-
Cry1
(
Proteintech
,
13474-1-AP
), anti-NeuN (
Merck Millipore
,
ABN90
),
(9)
NOX4 promotes ferroptosis of astrocytes by oxidative stress-induced lipid peroxidation via the impairment of mitochondrial metabolism in Alzheimer's diseases
Redox biology
May 1, 2021
Park MW, Cha HW, Kim J, Kim JH, Yang H, Yoon S, Boonpraman N, Yi SS, Yoo ID, Moon JS.
(PS1). 2.3 Reagents and antibodies The following antibodies were used: polyclonal rabbit anti-
NOX4
(
ab3517
,
Abcam
, Cambridge, UK), polyclonal rabbit anti-4-HNE antibody (
ab3517
, Abcam, Cambridge, UK), polyclonal
(10)
Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells
eLife
April 23, 2021
Ogino T, Matsunaga N, Tanaka T, Tanihara T, Terajima H, Yoshitane H, Fukada Y, Tsuruta A, Koyanagi S, Ohdo S.
gels, transferred to polyvinylidene difluoride
membranes
, and probed with antibodies against Clock (
ab3517
;
Abcam
, Cambridge, UK), E-cadherin (
AF748
;
R and D systems
, Minneapolis, MN), Claudin1 (13050–1-AP;
(11)
Epigallocatechin‑3‑gallate inhibits self‑renewal ability of lung cancer stem‑like cells through inhibition of CLOCK
International journal of molecular medicine
December 1, 2020
Jiang P, Xu C, Zhang P, Ren J, Mageed F, Wu X, Chen L, Zeb F, Feng Q, Li S.
for 1 h at room temperature. The following primary antibodies were used: Anti-CLOCK (
1:1,000
; cat no.
ab3517
,
Abcam
), anti-
CD133
(
1:1,000
; cat no.
ab216323
,
Abcam
), anti-
CD44
(
1:1,000
; cat no.
ab189524
,
Abcam
),
(12)
Elevated CLOCK and BMAL1 Contribute to the Impairment of Aerobic Glycolysis from Astrocytes in Alzheimer's Disease
International journal of molecular sciences
October 23, 2020
Yoo ID, Park MW, Cha HW, Yoon S, Boonpraman N, Yi SS, Moon JS.
USA). 4.2. Reagents and Antibodies The following antibodies were used: polyclonal rabbit anti-CLOCK (
ab3517
,
Abcam
, (Cambridge, UK)), polyclonal rabbit anti-BMAL1 antibody (
NB100-2288
,
Novus Biologicals
), monoclonal
(13)
Chronic Timed Sleep Restriction Attenuates LepRb-Mediated Signaling Pathways and Circadian Clock Gene Expression in the Rat Hypothalamus
Frontiers in Neuroscience
September 8, 2020
Qi Sun, Yang Liu, Wei Wei, Dan Wu, Ren Lin, Deliang Wen, Lihong Jia
procured from
Cell Signaling Technology
(MA, United States). The primary antibodies against CLOCK (#
ab3517
) and phosphorylated-
PI3K
(p-
PI3K
, Y607, #
ab182651
) were purchased from
Abcam
(Cambridge, MA, United
(14)
Circadian Clock Regulation of Developmental Time in the Kidney
Cell reports
May 19, 2020
Dan H, Ruan T, Sampogna RV.
AF1556
; RRID:AB_354858 Aquaporin-2,
Sigma-Aldrich
,Cat#
A7310
; RRID:AB_476762 Clock for ChIP,
Abcam
,Cat#
AB3517
; RRID:AB_303866
Bmal1
Rabbit,
Novus
,Cat#
NB100–2288
; RRID:AB_10000794 Alexa Fluor 488
rat
secondary
(15)
Noninvasive 40-Hz Light Flicker Rescues Circadian Behavior and Abnormal Lipid Metabolism Induced by Acute Ethanol Exposure via Improving SIRT1 and the Circadian Clock in the Liver-Brain Axis
Frontiers in Pharmacology
March 25, 2020
Youli Yao, Wenjiang Zhang, Ruibo Ming, Qiyu Deng, Along Zuo, Shengli Zhang, Ying Ying, Yingying Zhao, Junxian Ma
recorded for 5 days. Antibodies and Reagents
Anti-SREBP1
(
ab28481
), anti-GAPDH (
ab8245
), anti-CLOCK (
ab3517
), anti-
BMAL1
(
ab228594
), and anti-
SIRT1
(
ab110304
) were purchased from
Abcam
(Cambridge, MA, USA). Antibodies
(16)
Circadian Regulator CLOCK Recruits Immune-Suppressive Microglia into the GBM Tumor Microenvironment
Cancer discovery
March 1, 2020
Chen P, Hsu WH, Chang A, Tan Z, Lan Z, Zhou A, Spring DJ, Lang FF, Wang YA, DePinho RA.
and 30 sec off). Solubilized
chromatin
was then incubated with a mixture of antibody [CLOCK (
Abcam
, #
ab3517
) or BAML1 (CST, #
14020S
)] and Dynabeads (Life Technologies) overnight. Immune complexes were then washed
(17)
Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation
Nature communications
December 18, 2019
Hasegawa S, Fukushima H, Hosoda H, Serita T, Ishikawa R, Rokukawa T, Kawahara-Miki R, Zhang Y, Ohta M, Okada S,
et al
.
Slurry (
Merck Millipore
) and then
immunoprecipitated
for 48 h at 4 °C using 5 μg of anti-CLOCK antibody (
ab3517
,
Abcam
, Cambridge, UK) or anti-rabbit IgG (
ab46540
,
Abcam
).
Chromatin
–antibody complexes were collected
(18)
2,3,7,8-Tetrachlorodibenzo-p-dioxin abolishes circadian regulation of hepatic metabolic activity in mice
Scientific Reports
December 1, 2019
Kelly A. Fader, Rance Nault, Claire M. Doskey, Russell R. Fling, Timothy R. Zacharewski
antibodies as previously described45: IgG (#
2729
,
Cell Signaling
),
ARNTL
(#
14020
, Cell Signaling), CLOCK (
ab3517
,
Abcam
, Cambridge, MA), or AhR (
BML-SA210
,
Enzo
, Farmingdale, NY). ChIP DNA was purified using a QIAquick
(19)
Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesis
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
June 1, 2019
Lixian Chen et al.
(
ab3505
),
MT1
(
ab203038
),
MT2
(
ab203346
), cyclin-dependent kinase inhibitor 1A (p16,
ab189043
), CLOCK (
ab3517
), desmin (
ab185033
) and CD68 (
ab31630
) were purchased from
Abcam
(Cambridge, MA);
mouse
CK-19 antibody
(20)
Nuclear Lamin B1 Interactions With Chromatin During the Circadian Cycle Are Uncoupled From Periodic Gene Expression
Frontiers in genetics
January 1, 2019
Brunet A, Forsberg F, Fan Q, Sæther T, Collas P.
Tris–HCl pH 7.6, 0.05%
Tween®20
).
Membranes
were incubated with antibodies against CLOCK (
1:500
;
Abcam
ab3517
), LMNB1 (
1:1,000
;
Santa Cruz Biotechnology
sc6216
), and β-actin (
1:2,000
;
Sigma-Aldrich
A5441
) in TBST
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Reviews
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(1)
12/14/2015
Anti-Clock Antibody
A R
We have used clock antibody to measure levels of clock protein in the human fibroblast cells at different time point.
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Anti-KAT13D / CLOCK antibody from Abcam
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