Anti-AMPK alpha 2 antibody ab3760 from Abcam

Product Specs
ItemAnti-AMPK alpha 2 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab3760
Antibody TypePrimary
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
IsotypeIgG
HostRabbit
Quantity50 UL
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NamePRKAA2
ReactivityHuman, Mouse
TargetAMPK alpha 2

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Myonectin protects against skeletal muscle dysfunction in male mice through activation of AMPK/PGC1α pathwayNat CommunAugust 4, 2023Yuta Ozaki, Koji Ohashi, Naoya Otaka, Hiroshi Kawanishi, Tomonobu Takikawa, Lixin Fang, Kunihiko Takahara, Minako Tatsumi, Sohta Ishihama, Mikito Takefuji, et al.Cell Signaling Technology. Antibodies against AMPKα1 (Cat. ab3759)(diluted at 1:1000), AMPKα2 (Cat. ab3760)(diluted at 1:1000) and total OXPHOS (Cat. ab110413)(diluted at 1:500) were purchased from Abcam. Antibody

(2) Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill runningJournal of Biological MethodsAugust 23, 2022Lisbeth Liliendal ValbjÃ¸rn MÃ¸ller, Steffen Henning Raun, Andreas MÃ¦chel Fritzen, Lykke Sylow(Cell Signaling Technology Cat. # 2535, RRID:AB_331250; 1:1000 in 2% skimmed milk; 15 μg protein loaded)AMPKα2 (Abcam Cat. # ab3760, RRID:AB_304055; 1:1000 in 2% skimmed milk; 15 μg protein loaded)p-p38MAPK T180/Y182 (Cell Signaling Technology

(3) Liquiritin Attenuates Pathological Cardiac Hypertrophy by Activating the PKA/LKB1/AMPK PathwayFrontiers in PharmacologyMay 3, 2022Xiahenazi Aiyasiding, Hai-Han Liao, Hong Feng, Nan Zhang, Zheng Lin, Wen Ding, Han Yan, Zi-Ying Zhou, Qi-Zhu TangCell Signaling Technology (Pudong, Shanghai, China). Anti-phospho-AMPKα2 (p-AMPKα2, ab109402) and anti-AMPKα2 (T-AMPKα2, ab3760) antibodies were purchased from Abcam (Cambridge, United Kingdom). Secondary antibodies were purchased

(4) Endothelial Adenosine Monophosphate-Activated Protein Kinase-Alpha1 Deficiency Potentiates Hyperoxia-Induced Experimental Bronchopulmonary Dysplasia and Pulmonary HypertensionAntioxidants (Basel, Switzerland)November 29, 2021Elsaie A, Menon RT, Shrestha AK, Gowda SH, Varghese NP, Barrios RJ, Blanco CL, Konduri GG, Shivanna B.subjected to immunoblotting using antibodies against: AMPKα1 (Abcam, Cambridge, UK; ab3759), AMPKα2 (Abcam; ab3760), and glyceraldehyde 3-phosphate dehydrogenase ([GAPDH] Cell Signaling, Danvers, MA, USA; 2118). 2.1.4.

(5) Brain-specific suppression of AMPKα2 isoform impairs cognition and hippocampal LTP by PERK-mediated eIF2α phosphorylationMolecular psychiatryJune 1, 2021Yang W, Zhou X, Zimmermann HR, Ma T.overnight at 4 °C with primary antibody for AMPKα1 (7.5 μg/mL, AF3197, R&D Systems) and AMPKα2 (1:400, ab3760, Abcam). Alexa Fluor 488 secondary antibody (Thermo Fisher Catalog #: R37120, RRID:AB_2556548) was used.

(6) circSAMD4A participates in the apoptosis and autophagy of dopaminergic neurons via the miR‑29c‑3p‑mediated AMPK/mTOR pathway in Parkinson's diseaseMolecular Medicine ReportsMay 27, 2021Wensheng Wang, Rongxiang Lv, Jingjing Zhang, Yu Liu(p)-5′AMP-activated protein kinase (AMPK; rabbit; 1:1,000; cat. no. ab23875; Abcam), AMPK (rabbit; 1;5,000; cat. no. ab3760; Abcam), p-mTOR (rabbit; 1:10,000; cat. no. ab137133; Abcam) and mTOR (rabbit; 1:10,000; cat. no. ab109268;

(7) Liquiritin Attenuates Lipopolysaccharides-Induced Cardiomyocyte Injury via an AMP-Activated Protein Kinase-Dependent Signaling PathwayFrontiers in PharmacologyMay 14, 2021Shan-Qi Mou et al.Cell Signaling Technology. Primary antibodies, including anti-phospho-AMPKα2 (p-AMPKα2, ab109402), anti-AMPKα2 (T-AMPKα2, ab3760), anti-phospho-IκBα (ab133462), anti-IκBα (ab7217), anti-phospho-p65 (ab194726), and anti-p65 (ab16502)

(8) Smooth muscle-specific HuR knockout induces defective autophagy and atherosclerosisCell death & diseaseApril 9, 2021Liu S, Jiang X, Cui X, Wang J, Liu S, Li H, Yang J, Zhang C, Zhang W.primary antibodies for HuR (29 ng/ml, 12582S, CST), AMPKα1 (1 μg/ml, Ab32047, Abcam), AMPKα2 (1 μg/ml, ab3760, Abcam), p-AMPK (27 ng/ml, 2535S, CST), p62 (293 ng/ml, 184201-AP, Proteintech), LC3I/II (64.5 ng/ml,

(9) Role of AMPK and Akt in triple negative breast cancer lung colonizationNeoplasiaApril 8, 2021Jeremy Johnson, Zeta Chow, Eun Lee, Heidi L. Weiss, B. Mark Evers, Piotr Rychahouin this study include: Abcam: (1) AMPKα1, ab32047 (1:1000 for WB and 1:250 for IHC) and (2) AMPKα2, ab3760 (1:1000 for WB and 1:500 for IHC); Cell Signaling Technology (Danvers, MA): (1) pAkt, #4060 (1:2000

(10) Protein expression alteration in hippocampus upon genetic repression of AMPKα isoformsHippocampusApril 1, 2021Wenzhong Yang, Xueyan Zhou, Xin Wang, Jingyun Lee, Dan Wu, Peiqing Sun, Cristina M. Furdui, Tao Mausing following primary antibodies: AMPKα1 (1:1000, Abcam, Cat# ab3759), AMPKα2 (1:1000, Abcam, Cat# ab3760), GAPDH (1:10,000, Cell Signaling, Cat# 2118S). For SUnSET protein synthesis assay, acute 400 μm transverse

(11) FGF1^ΔHBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppressionSignal transduction and targeted therapyMarch 24, 2021Wang D, Yin Y, Wang S, Zhao T, Gong F, Zhao Y, Wang B, Huang Y, Cheng Z, Zhu G, et al.cat# ab45174, Abcam), phospho-AMPK (1:1000, cat# 50081, Cell Signaling Technology), AMPK (1:1000, cat# ab3760, Abcam) and SIRT1 (1:1000, cat# ab110304, Abcam). The immunoreactive bands were detected with secondary

(12) Knockout of AMPKα2 Blocked the Protection of Sestrin2 Overexpression Against Cardiac Hypertrophy Induced by Pressure OverloadFrontiers in pharmacologyJanuary 1, 2021Zhang N, Liao HH, Feng H, Mou SQ, Li WJ, Aiyasiding X, Lin Z, Ding W, Zhou ZY, Yan H, et al.P-acetyl-CoA carboxylase antibody (#3661S). ABCAM provided the following primary antibodies: Anti-AMPKα2 (ab3760), p-AMPKα2 (S491, ab109402), anti-SOD1 (ab16831), anti-SOD2 (ab38155), Nrf2 (ab15323), 4-hydroxynonenal

(13) Knockdown of AMPKα2 impairs epithelial‑mesenchymal transition in rat renal tubular epithelial cells by downregulating ETS1 and RPS6KA1Molecular medicine reportsDecember 1, 2020Yin X, Ma F, Fan X, Zhao Q, Liu X, Yang Y.antibodies overnight at 4°C. Primary antibodies included AMPKα2 (polyclonal rabbit; 1:2,000; product code ab3760; Abcam), v-ets erythroblastosis virus E26 oncogene homolog-1 (ETS-1) (monoclonal rabbit; 1:1,000; product

(14) A MicroRNA Linking Human Positive Selection and Metabolic DisordersCellOctober 29, 2020Wang L, Sinnott-Armstrong N, Wagschal A, Wark AR, Camporez JP, Perry RJ, Ji F, Sohn Y, Oh J, Wu S, et al.alpha,Abcam,ab24509 UCP3,Abcam,ab3477 Insulin Receptor,Abcam,ab137747 IRS1,Abcam,ab52167 β-Tubulin,Sigma,T8328 AMPK alpha 2,Abcam,ab3760 AMPK alpha 2 (phospho S491),Abcam,ab109402 PPAR gamma,Abcam,ab45036 Acetyl-CoA Carboxylase,Cell Signaling,#3662 Phospho-Acetyl-CoA

(15) Activation of LKB1 rescues 3T3-L1 adipocytes from senescence induced by Sirt1 knock-down: a pivotal role of LKB1 in cellular agingAging (Albany NY)October 10, 2020Fan Lan, Yan Lin, Zhenfeng Gao, Jose M. Cacicedo, Karen Weikel, Yasuo Idofrom Merck (USA); Sirt1 (07-131), from Abcam (USA); FABP4 (ab23693), OXPHOS (ab110413), AMPK alpha2 (ab3760). CamKK β antibody was a gift from D. Grahame Hardie (Dundee, UK). Construction of recombinant lentiviruses:

(16) Targeting PI3K and AMPKα Signaling Alone or in Combination to Enhance Radiosensitivity of Triple Negative Breast CancerCellsMay 19, 2020Jeremy Johnson, Zeta Chow, Dana Napier, Eun Lee, Heidi L. Weiss, B. Mark Evers, Piotr Rychahouab32047 (1:1000 for WB, 1:100 for IHC for PDXs, and 1:175 for IHC for whole tissue sections), (2) AMPKα2, ab3760 (1:1000 for WB, 1:200 for IHC for PDXs, and 1:300 for IHC for whole tissue sections), (3) cyclin D1,

(17) Down-regulation of AMPK signaling pathway rescues hearing loss in TFB1 transgenic mice and delays age-related hearing lossAgingApril 2, 2020Zhao J, Li G, Zhao X, Lin X, Gao Y, Raimundo N, Li GL, Shang W, Wu H, Song L.Beverly, MA, USA) at 1:100, anti-AMPKα1 (ab32047, Abcam, Cambridge, England, UK) at 1:200 and anti-AMPKα2 (ab3760, Abcam, Cambridge, England, UK) at 1:100, respectively. Then incubated in the secondary antibodies HRP-labeled

(18) miR-4999-5p Predicts Colorectal Cancer Survival Outcome and Reprograms Glucose Metabolism by Targeting PRKAA2OncoTargets and TherapyFebruary 11, 2020Qiwei Zhang, Zhi Hong, Jieyao Zhu, Chao Zeng, Zhen Tang, Weiqiang Wang, He Huangblocked with 2% bovine serum albumin for 1 h, and incubated with primary antibodies against AMPKα2 (ab3760, Abcam, Cambridge, MA, USA; 1:100) or Ki-67 (#9027, Cell Signaling Technology, Beverly, MA, USA; 1:400)

(19) Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPKCell ReportsNovember 5, 2019Yu Wang et al.RRID:AB_10950498 Anti-AMPK alpha 1,Abcam,Cat# ab3759; RRID:AB_304054 Anti-AMPK alpha 2,Abcam,Cat# ab3760; RRID:AB_304055 Anti-AMPK gamma 1,Abcam,Cat# ab32508; RRID:AB_722769 Goat Anti-Rabbit IgG (H L)-HRP Conjugate

(20) AMPK Signaling Regulates the Age-Related Decline of Hippocampal NeurogenesisAging and diseaseOctober 1, 2019Brian Z Wang, Jane J Yang, Hongxia Zhang, Charity A Smith, Kunlin JinMillipore-Sigma (MO, USA). Primary antibodies used are as follows: AMPKα1 and AMPKα2 (Cat. # ab3759, ab3760, respectively, both 1:200; Abcam, USA), AMPKβ1 and AMPKβ2 (Cat.# orb37351, orb381985, respectively,

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Anti-AMPK alpha 2 antibody from Abcam

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Anti-AMPK alpha 2 antibody from Abcam

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