Anti-NFAT1 antibody [25A10.D6.D2] ab2722 from Abcam

Product Specs
ItemAnti-NFAT1 antibody [25A10.D6.D2]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2722
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityMonoclonal
Clone25A10.D6.D2
HostMouse, Nude mouse
Quantity100 UG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameNFATC2
ReactivityHuman
TargetNFAT1
IsotypeIgG1

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) AS-605240 Blunts Osteoporosis by Inhibition of Bone ResorptionDrug Design, Development and TherapyApril 27, 2023Jiacheng Sun, Guoping Cai, Jinlong Shen, Pu Cheng, Jiapeng Zhang, Dengteng Jiang, Xianquan Xu, Fangying Lu, Lihua Chen, Haixiao Chen1:1000) from Cell Signaling Technology (Danvers, USA). We obtained c-Fos (ab222699, 1:1000) and NFATc1 (ab2722, 1:1000) from Abcam (Shanghai, China), and the dilution of the primary antibodies was obtained from

(2) Neuroinflammation aggravated by traumatic brain injury at high altitude is reversed by L-serine via NFAT1-mediated microglial polarizationFrontiers in Cellular NeuroscienceApril 14, 2023Jinchun Liu, Shunhua Peng, Lisha Ye, Yechao Sun, Qiong Zhao, Hua Wei, Qianqian Luo, Min He, Guohua Wanga Bio-Rad system. The blots were blocked in 5% nonfat milk and incubated with anti-NFAT-1 (1:1,000, Ab2722; Abcam, United States) and β-actin (1:10,000, A2228; Sigma–Aldrich, St. Louis, MO, United States) primary

(3) NFAT1 Orchestrates Spinal Microglial Transcription and Promotes Microglial Proliferation via c‐MYC Contributing to Nerve Injury‐Induced Neuropathic PainAdvanced ScienceSeptember 1, 2022Bao‐Chun Jiang, Ting‐Yu Ding, Chang‐Yun Guo, Xue‐Hui Bai, De‐Li Cao, Xiao‐Bo Wu, Wei‐Lin Sha, Ming Jiang, Long‐Jun Wu, Yong‐Jing Gao5% milk and incubated overnight at 4°C with antibodies against NFAT1 (mouse, 1:1000, Abcam, catalog #Ab2722; Rabbit, 1:200, Cell Signaling Technology, catalog #4389S) and c‐MYC (mouse, 1:250, Santa Cruz, catalog

(4) SNPs for Genes Encoding the Mitochondrial Proteins Sirtuin3 and Uncoupling Protein 2 Are Associated With Disease Severity, Type 2 Diabetes, and Outcomes in Patients With Pulmonary Arterial Hypertension and This Is Recapitulated in a New Mouse Model Lacking Both GenesJournal of the American Heart AssociationDecember 7, 2021Yongneng Zhang et al.CD4 (Invitrogen, LS14004182), Ki67 (Abcam, ab16667), nuclear factor of activated T‐cells c2 (Abcam, ab2722), hypoxia inducible factor 1α (Abcam, 51608), and Y705p‐STAT3 (Cell Signaling Technology, 9131). All

(5) Inhibition of Lipopolysaccharide-Induced Inflammatory Bone Loss by Saikosaponin D is Associated with Regulation of the RANKL/RANK PathwayDrug Design, Development and TherapyNovember 23, 2021Xinhui Wu, Kangxian Zhao, Xiaoxin Fang, Feng Lu, Weikang Zhang, Xiaoting Song, Lihua Chen, Jiacheng Sun, Haixiao Chenprovided by Cell Signaling Technology (1:1000, Danvers, USA). In addition, c-Fos(ab222699) and NFATc1(ab2722) were obtained from Abcam (1:1000, Cambridge, UK), and the dilution of the primary antibodies was purchased

(6) Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional responseNature communicationsOctober 8, 2021Gajewska KA, Lescesen H, Ramialison M, Wagstaff KM, Jans DA.1:100) and anti-EIF1A (Cat no. 38976, Abcam, 1:200) antibodies, and mouse monoclonal anti-NFAT1 (Cat no. ab2722 1:1000, Abcam), anti-Actin (Cat no. ab3280, Abcam, 1:4000), anti-UBC9 (Cat no. 610749, BD, 1:100) and

(7) Propofol maintains Th17/Treg cell balance and reduces inflammation in rats with traumatic brain injury via the miR‑145‑3p/NFATc2/NF‑κB axisInternational Journal of Molecular MedicineMay 21, 2021Can Cui et al.primary antibodies as follows: Recombinant nuclear factor of activated T cells c2 (NFATc2) (1:1,000, ab2722; Abcam), p65 (1:1,000, ab16502; Abcam), p‑p65 (1:2,000, ab86299; Abcam), Iκ Bα (1:1,000, ab32518; Abcam),

(8) Cell shape regulates subcellular organelle location to control early Ca2+ signal dynamics in vascular smooth muscle cellsScientific ReportsDecember 1, 2020R. C. Calizo, M. K. Bell, A. Ron, M. Hu, S. Bhattacharya, N. J. Wong, W. G. M. Janssen, G. Perumal, P. Pederson, S. Scarlata, et al.acetylcholine receptor 3 (M3R),IF 1:100,"Abcam, cat # ab126168" NFATc1 antibody,IF 1:100,"Abcam, cat # ab2722" SRF antibody,IF 1:100,"Cell signaling, cat # 4261" Myocardin,IF 1:100,"Abcam, cat # 22073"

(9) LncRNA Nron regulates osteoclastogenesis during orthodontic bone resorptionInternational Journal of Oral ScienceDecember 1, 2020Ruilin Zhang, Junhui Li, Gongchen Li, Fujun Jin, Zuolin Wang, Rui Yue, Yibin Wang, Xiaogang Wang, Yao SunChina) was used to block the cells. Subsequently, the cells were co-incubated with anti-Nfatc1 antibody (ab2722; dilution 1:400; Abcam, USA) and anti-actin antibody (ab8227; dilution 1:500, Abcam, USA) overnight

(10) FOXO1 promotes HIV latency by suppressing ER stress in T cellsNature microbiologySeptember 1, 2020Vallejo-Gracia A, Chen IP, Perrone R, Besnard E, Boehm D, Battivelli E, Tezil T, Krey K, Raymond KA, Hull PA, et al.against RNA Pol II (39097, Active Motif), RelA (A301–824A, Bethyl), ATF4 (11815, Cell Signaling), NFAT1 (ab2722, Abcam), or IgG control (P120–101, Bethyl). After incubation with protein A/G Dynabeads for 2 h and

(11) The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of AsthmaJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine ResearchApril 1, 2020Shi R, Bian X, Feng S, Yang X, Zhao T, Guo M.fat-free milk for 1 h. Then, the membranes were dividedly incubated with primary antibodies, anti-NFAT (no. ab2722; Abcam, UK), anti-SERCA2 (no. ab2817; Abcam), anti-guanine nucleotide-binding protein subunit alpha-12

(12) Increased HERV-E clone 4–1 expression contributes to DNA hypomethylation and IL-17 release from CD4+ T cells via miR-302d/MBD2 in systemic lupus erythematosusCell Communication and SignalingDecember 1, 2019Xin Wang, Chaoshuai Zhao, Chengzhong Zhang, Xingyu Mei, Jun Song, Yue Sun, Zhouwei Wu, Weimin Shipolyvinylidene difluoride (PVDF) membrane (Millipore, USA). Antibodies used in the assays were NFAT1 antibody (ab2722, Abcam, Cambridge, UK), ER-α antibody (#8644, Cell Signaling Technology) and GAPDH antibody (#5174,

(13) Down-regulated HSDL2 expression suppresses cell proliferation and promotes apoptosis in papillary thyroid carcinomaBioscience ReportsJune 28, 2019Jing Zeng, Xiao Ma, Jinjing Wang, Ran Liu, Yun Shao, Yanwei Hou, Zhiyuan Li, Yi Fangincubated with first antibodies against AKT3 (1:1000, CST#9272, Boston, U.S.A.), NFATc2 (1:500, Abcam ab2722, Cambridge, U.K.), PPP3CA (1:2000, Abcam ab3673, Cambridge, U.K.), FOS (1:200, Abcam ab7963, Cambridge,

(14) FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axisEBioMedicineMarch 1, 2019Wu L, Zhang D, Zhou L, Pei Y, Zhuang Y, Cui W, Chen J.sonicated into 100- to 500-bp fragments. Soluble chromatin was precipitated with anti-NFAT1 (Abcam: ab2722) or a control non-immune IgG (Cell Signaling Technology: #2729). For real-time ChIP-PCR, the SYBR green

(15) Upregulation of Ets1 expression by NFATc2 and NFKB1/RELA promotes breast cancer cell invasivenessOncogenesisNovember 23, 2018Kim GC, Kwon HK, Lee CG, Verma R, Rudra D, Kim T, Kang K, Nam JH, Kim Y, Im SH.

(16) Activation of Th2 cells downregulates CRTh2 through an NFAT1 mediated mechanismPLOS ONEJuly 3, 2018Emily MacLean Scott, Lauren A. Solomon, Courtney Davidson, Jessica Storie, Nami Shrestha Palikhe, Lisa Cameron10μg target antibody for 20 minutes at room temperature with rotation. Antibodies used were antiNFAT1 (Ab2722, Abcam, Cambridge, MA, USA), anti-GATA3 (sc-269, Santa Cruz Biotechnology, CA, USA) and mouse IgG (M5284,

(17) NFAT1-regulated IL6 signalling contributes to aggressive phenotypes of gliomaCell Communication and SignalingDecember 19, 2017Yang Jiang, Sheng Han, Wen Cheng, Zixun Wang, Anhua Wuthen incubated with anti-IL6 (1:2000, ab6672, Abxam), IL6R (1:1000, ab128008, Abcam) or NFAT1 (1:1000, ab2722, Abcam) at 4°C overnight. Membranes were washed four times with TBST and incubated with the appropriate

(18) The cardiac calsequestrin gene transcription is modulated at the promoter by NFAT and MEF-2 transcription factorsPLOS ONESeptember 8, 2017Rafael Estrada-Avilés, Gabriela Rodríguez, Angel Zarain-Herzberg

(19) Lithium enhances the antitumour effect of temozolomide against TP53 wild-type glioblastoma cells via NFAT1/FasL signallingBritish Journal of CancerMay 9, 2017Sheng Han, Lingxuan Meng, Yang Jiang, Wen Cheng, Xinxin Tie, Junzhe Xia, Anhua Wu(D17D2; Cell Signaling Technology), p53 (ab1101; Abcam, Cambridge, UK), Fas (ab103551; Abcam), NFAT1 (ab2722; Abcam) and FasL (all at 1 : 1000 dilution). The membrane was then incubated with the appropriate secondary

(20) Bezafibrate Attenuates Pressure Overload-Induced Cardiac Hypertrophy and FibrosisPPAR ResearchJanuary 3, 2017Si-Chi Xu, Zhen-Guo Ma, Wen-Ying Wei, Yu-Pei Yuan, Qi-Zhu Tanganti-phospho-AMPK𝛼 (#2535). Anti-GAPDH (#ab8245), anticalcineurin (CaN) (#ab90540), and antiNFAT1 (#ab2722) were obtained from ABCAM. Anti-𝛼actinin was acquired from Millipore. The secondary antibodies were

Be the First to Write a Review!

Anti-NFAT1 antibody [25A10.D6.D2] from Abcam

Abcam

Citations (44)

Reviews

Anti-NFAT1 antibody [25A10.D6.D2] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map