Anti-Perforin antibody [CB5.4] ab16074 from Abcam

Product Specs
ItemAnti-Perforin antibody [CB5.4]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab16074
Antibody TypePrimary
IsotypeIgG2a
Quantity50 UG
HostRat
ApplicationsImmunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunoprecipitation, Western blot
ClonalityMonoclonal
CloneCB5.4
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NamePRF1
ReactivityMouse
TargetPerforin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapyNature CommunicationsNovember 9, 2023Zhiren Wang, Wenpan Li, Yanhao Jiang, Tuyen Ba Tran, Leyla Estrella Cordova, Jinha Chung, Minhyeok Kim, Georg Wondrak, Jennifer Erdrich, Jianqin Lugamma (ab9657, 1/200), anti-CD8α (ab209775, 1/100), anti-granzyme B (ab4059, 1/100) and anti-perforin (ab16074, 1/600) were purchased from Abcam; anti-NK1.1 (# MA1-70100, 1/100) and anti-CD69 (# PA5-102562, 1/100)

(2) EGCG Inhibits Tumor Growth in Melanoma by Targeting JAK-STAT Signaling and Its Downstream PD-L1/PD-L2-PD1 Axis in Tumors and Enhancing Cytotoxic T-Cell ResponsesPharmaceuticals (Basel, Switzerland)October 26, 2021Ravindran Menon D, Li Y, Yamauchi T, Osborne DG, Vaddi PK, Wempe MF, Zhai Z, Fujita M.ab203035), anti-granzyme B (2C5) (1:50; Santa Cruz, Cat: sc-8022), anti-perforin (1:200; Abcam Cat: ab16074, Waltham, MA, USA) and anti-PD-L1 (1:100; eBioscience, Cat:14-5983-82, Waltham, MA, USA). The slides

(3) CD8⁺ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cellsNature communicationsJuly 22, 2021Koda Y, Teratani T, Chu PS, Hagihara Y, Mikami Y, Harada Y, Tsujikawa H, Miyamoto K, Suzuki T, Taniki N, et al.Granzyme B antibody (1/100, R& D systems, AF1865), and anti-mouse Perforin antibody (1/100, Abcam, Ab16074) were used as primary antibody. After washing with PBS, the sections were incubated with Alexa Fluor

(4) Combination Chemo-Immunotherapy for Pancreatic Cancer Using the Immunogenic Effects of an Irinotecan Silicasome Nanocarrier Plus Anti-PD-1Advanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2021Liu X, Jiang J, Liao YP, Tang I, Zheng E, Qiu W, Lin M, Wang X, Ji Y, Mei KC, et al.from ThermoFisher; CRT (ab2907), while antibodies to HMGB1 (ab18256), granzyme B (ab4059), perforin (ab16074) and IFN‐γ (ab9657) were purchased from Abcam. The antibody to LC‐3 (#0231‐100/LC3‐5F10) was purchased

(5) MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphomaJournal of Experimental & Clinical Cancer ResearchDecember 1, 2020Yangyang Xu, Zhenchuan Liu, Lixin Lv, Ping Li, Bing Xiu, Wenbin Qian, Aibin Liangab270264), anti-perforin (Abcam, ab47225), anti-Granzyme B (BOSTER, A00353), anti-perforin (Abcam, ab16074). The following secondary antibodies were used: goat anti-mouse (CST) and goat anti-rabbit (CST). MG132

(6) Liposomal Delivery of Mitoxantrone and a Cholesteryl Indoximod Prodrug Provides Effective Chemo-immunotherapy in Multiple Solid TumorsACS nanoOctober 27, 2020Mei KC, Liao YP, Jiang J, Chiang M, Khazaieli M, Liu X, Wang X, Liu Q, Chang CH, Zhang X, et al.from ThermoFisher; (ii) anti-CRT (#ab2907, 1: 200), anti-HMGB1 (#ab18256, 1: 200), anti-perforin (#ab16074, 1:100), anti-granzyme B (#ab4059, 1: 100), and anti-IFN-γ (#ab9697, 1: 200) were purchased from Abcam;

(7) Breast Cancer Chemo-immunotherapy through Liposomal Delivery of an Immunogenic Cell Death Stimulus Plus Interference in the IDO-1 PathwayACS nanoNovember 27, 2018Lu J, Liu X, Liao YP, Wang X, Ahmed A, Jiang W, Ji Y, Meng H, Nel AE.1/200) were from eBioscience; anti-CRT (ab2907, 1/50), anti-LRP1(CD91) (ab92544, 1/50), antiperforin (ab16074, 1/100), and anti-PD-1 (ab214421, 1/1000) were from Abcam; anticleaved caspase 3 antibody (#9664, 1/200)

(8) Nano-enabled pancreas cancer immunotherapy using immunogenic cell death and reversing immunosuppressionNature communicationsNovember 27, 2017Lu J, Liu X, Liao YP, Salazar F, Sun B, Jiang W, Chang CH, Jiang J, Wang X, Wu AM, et al.anti-HMGB-1 (ab18256, 1/200), anti-LRP1(CD91) (ab92544, 1/50), anti-TLR4 (ab13867, 1/50), and anti-perforin (ab16074, 1/100). Anti-CD8 (#14-0808, 1/100), anti-Foxp3 (#13-5773, 1/200) and anti-IL-10 (#14-7101, 1/50) were

(9) Natural cytotoxicity receptor 1 in mouse uNK cell maturation and functionMucosal ImmunologySeptember 1, 2017A M Felker, B A Croyrabbit anti-mouse VEGFA (Abcam, Toronto, ON, Canada; ab46154) or 40 mg ml 1 rat anti-mouse PRF (Abcam; ab16074) and 4 mg ml 1 goat anti-mouse GZMA (Santa Cruz Biotech, Dallas, TX; sc5510), or for 2 h (room temperature)

(10) Antibody-Dependent Cell-Mediated Cytotoxicity Epitopes on the Hemagglutinin Head Region of Pandemic H1N1 Influenza Virus Play Detrimental Roles in H1N1-Infected MiceFrontiers in ImmunologyMarch 21, 2017Zi-Wei Ye et al.

(11) Inactivation of maternal Hif-1α at mid-pregnancy causes placental defects and deficits in oxygen delivery to the fetal organs under hypoxic stressDevelopmental BiologyFebruary 1, 2017Doreswamy Kenchegowda, Bryony Natale, Maria A. Lemus, David R. Natale, Steven A. Fisherfor 1 hour at room temperature. Sections were incubated with anti-Ki67 (ab16667) or anti-Perforin (ab16074) primary antibodies (Abcam) at 1:300 at room temperature for 1 hour. The remainder of the procedure

(12) Interleukin-15 is required for immunosurveillance and immunoprevention of HER2/neu-driven mammary carcinogenesisBreast Cancer ResearchDecember 1, 2015Stefania Croci et al.determined through immunohistochemistry with anti-CD3 (Ab828; Abcam, Cambridge, UK) and anti-perforin (Ab16074, Abcam) antibodies as previously described [16]. Immunostaining was developed using alkaline phosphatase-conjugated

(13) In vivo RNA interference screens identify regulators of antiviral CD4(+) and CD8(+) T cell differentiationImmunityAugust 21, 2014Chen R, Bélanger S, Frederick MA, Li B, Johnston RJ, Xiao N, Liu YC, Sharma S, Peters B, Rao A, et al.T1 (sc-10750), anti-Blimp1 (sc-47732), anti-Cdk9 (sc-484) (Santa Cruz Biotechnology), anti-Perforin (ab16074) and anti-beta Actin (Abcam ab8227). Supplementary Material

(14) Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cellsPloS oneJanuary 1, 2014Workman AM, Jacobs AK, Vogel AJ, Condon S, Brown DM.

(15) MicroRNA-directed program of cytotoxic CD8+ T-cell differentiationProceedings of the National Academy of Sciences of the United States of AmericaNovember 12, 2013Trifari S, Pipkin ME, Bandukwala HS, Äijö T, Bassein J, Chen R, Martinez GJ, Rao A.

(16) DBA-lectin reactivity defines mouse uterine natural killer cell subsets with biased gene expressionBiology of reproductionOctober 1, 2012Zhilin Chen, Jianhong Zhang, Kota Hatta, Patricia D A Lima, Hakim Yadi, Francesco Colucci, Aureo T Yamada, B Anne Croy

(17) Ablation of Tpbpa-positive trophoblast precursors leads to defects in maternal spiral artery remodeling in the mouse placentaDevelopmental biologyOctober 1, 2011Dong Hu, James C Cross

(18) Preclinical Models for Neuroblastoma: Establishing a Baseline for TreatmentPLoS ONEApril 29, 2011Tal Teitz et al.MO762) (dilution 1∶40), NSE (DAKO, CA, USA, cat. MO873) (dilution 1∶50), Perforin (Abcam, MA, USA, cat. ab16074) (dilution1∶4,000), PGP9.5 (Morphosystems, NC, USA, cat. 7863-0504) (dilution1∶10,000), Synaptophysin,

(19) Antigen-specific clonal expansion and cytolytic effector function of CD8+ T lymphocytes depend on the transcription factor Bcl11bThe Journal of experimental medicineAugust 2, 2010Zhang S, Rozell M, Verma RK, Albu DI, Califano D, VanValkenburgh J, Merchant A, Rangel-Moreno J, Randall TD, Jenkins NA, et al.

(20) Cytotoxic CD8+ T Cell-Neuron Interactions: Perforin-Dependent Electrical Silencing Precedes But Is Not Causally Linked to Neuronal Cell DeathJournal of NeuroscienceDecember 9, 2009Sven G Meuth et al.OT-I (perf⫺/⫺) T cells using rabbit anti-granzyme B (1:100; ab4059; Abcam), rat anti-perforin (1:100; ab16074; Abcam), and the corresponding secondary antibodies goat anti-rabbit Cy3 and goat anti-rat Cy2 (both

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Anti-Perforin antibody [CB5.4] from Abcam

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Anti-Perforin antibody [CB5.4] from Abcam

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