Fig 2: Elevated expression of ADH1 and ADH4 proteins in NASH livers.(A) Western blot analyses were performed with lysates prepared from NASH livers and NCs. The NCs were of normal BMI and free from steatosis. Separate blots were probed for ADH1, ADH4 and ß-actin as these proteins all migrate at about 43 kDa. While similar signals for actin were detected for all samples, the NASH livers exhibited stronger signals for both ADH1 and ADH4. (B) The Western blot results were quantitated with NIHimage software. The normalized quantities of the ADH1 and ADH4 signals were the densities of the ADH bands divided by that of the ß-actin bands, respectively. The mean values for NASH and NC were plotted with error bars representing the standard errors of the means.