Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker ab11316 from Abcam

Product Specs
ItemAnti-gamma Tubulin antibody [GTU-88] - Centrosome Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab11316
Antibody TypePrimary
Quantity50 UL
HostMouse, Nude mouse
ApplicationsWestern blot
CloneGTU-88
ClonalityMonoclonal
IsotypeIgG1
Conjugate/TagUnconjugated
Concentration1.3 mg/ml
Gene NameTUBG2
ReactivityAfrican green monkey, Chicken, Chinese hamster, Cow, Dog, Human, Mouse
Targetgamma Tubulin

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Skewed X-inactivation is associated with retinal dystrophy in female carriers of RPGR mutationsLife Sci AllianceAugust 4, 2023Muhammad Usman, Christoph Jüschke, Fei Song, Dennis Kastrati, Marta Owczarek-Lipska, Jannis Eilers, Laurenz Pauleikhoff, Clemens Lange, John Neidhardtantibodies either were anti-RPGR antibody (1:200, HPA001593; Sigma-Aldrich), anti-gamma tubulin (1:500, ab11316; Abcam), anti-detyrosinated tubulin (1:500, ab3201; Merck, Millipore), or anti-polyglutamylation tubulin

(2) Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomesNucleic acids researchJune 9, 2023Leem J, Kim JS, Oh JS.Cell Signaling), anti-HEC1 (1:250, ab3613, Abcam) anti-p-T210-PLK1 (1:250, ab39068, Abcam), anti-γ-tubulin (1:250, ab11316, Abcam), and anti-GFP (1:500, ab1218, Abcam). After being washed three times, the oocytes were incubated

(3) Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranesNature communicationsJune 9, 2023Hildebrandt RP, Moss KR, Janusz-Kaminska A, Knudson LA, Denes LT, Saxena T, Boggupalli DP, Li Z, Lin K, Bassell GJ, et al.mouse anti-Myc (1:100, Developmental Studies Hybridoma Bank, 9E10), mouse anti-γ-Tubulin (1:500, Abcam, ab11316), rabbit anti-FLAG (1:1000, Cell Signaling Technologies, D6W5B), mouse anti-KIF1B - recognizes KIF1Bβ

(4) Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2BMC molecular and cell biologyMay 26, 2023Bieder A, Chandrasekar G, Wason A, Erkelenz S, Gopalakrishnan J, Kere J, Tapia-Páez I.lab), 1:20; mouse anti-acetylated tubulin, 1:5000, Sigma T7451; mouse anti-gamma-tubulin, 1:500, Abcam, ab11316). Antibodies have been previously validated for specificity [16, 18, 35]. Cells were incubated for 1

(5) Calcineurin associates with centrosomes and regulates cilia length maintenanceJournal of Cell ScienceApril 15, 2023Eirini Tsekitsidou, Cassandra J. Wong, Idil Ulengin-Talkish, Angela I. M. Barth, Tim Stearns, Anne-Claude Gingras, Jennifer T. Wang, Martha S. CyertRRID:AB_537701); anti-CNB (1:100; Sigma-Aldrich, C0581; RRID:AB_258693), anti-gamma tubulin (1:500; Abcam, ab11316; RRID:AB_297920), anti-myc-tag (1:300; Cell Signaling Technology, 2278; RRID:AB_490778), anti-POC5 (1:100;

(6) Deficiency of the minor spliceosome component U4atac snRNA secondarily results in ciliary defects in human and zebrafishProceedings of the National Academy of Sciences of the United States of AmericaFebruary 28, 2023Khatri D, Putoux A, Cologne A, Kaltenbach S, Besson A, Bertiaux E, Guguin J, Fendler A, Dupont MA, Benoit-Pilven C, et al.following primary antibodies were used: Arl13b (1:500, 17711-1 AP, Proteintech), γ-tubulin (1:250, ab11316, Abcam), acetylated α-tubulin (1:1,000, T6793, Sigma), and AC3 (1:500, PA5-35382, Invitrogen), diluted

(7) RBM4 regulates cellular senescence via miR1244/SERPINE1 axisCell death & diseaseJanuary 13, 2023Wang L, Zhang X, Sheng J, Chen L, Zhi L, Zheng Q, Qi Y, Wang L, Zhang J, Zhao J, et al.#DCS83), Cyclin D3 (Cell signaling, #DCS22), SERPINE1 (Proteintech, 13801-1-AP) and tubulin (Abcam, ab11316). RNA isolation and RT-qPCR RNA was extracted from cells using Trizol (Invitrogen) according to the

(8) The LRRK2 signaling network converges on a centriolar phospho-Rab10/RILPL1 complex to cause deficits in centrosome cohesion and cell polarizationBiology openAugust 15, 2022Lara Ordóñez AJ, Fasiczka R, Fernández B, Naaldijk Y, Fdez E, Blanca Ramírez M, Phan S, Boassa D, Hilfiker S.1:100), mouse monoclonal anti-LAMP2 (Santa Cruz, sc-18822, 1:50), mouse monoclonal anti-γ-tubulin (Abcam, ab11316, 1:1000), rabbit polyclonal anti-RILPL1 (Sigma-Aldrich, HPA-041314, 1:300), sheep polyclonal anti-RILPL1

(9) Padi2/3 Deficiency Alters the Epigenomic Landscape and Causes Premature Differentiation of Mouse Trophoblast Stem CellsCellsAugust 9, 2022Noura N. Ballasy, Elizabeth A. Bering, Caroline Kokorudz, Bethany N. Radford, Xiang Zhao, Wendy Dean, Myriam HembergerFremont, CA, USA); SOX2 1:250 (R&D Systems AF2018, Minneapolis, MN, USA); gamma-Tubulin 1:100 (Abcam ab11316, Cambridge, UK); alpha-Tubulin 1:100 (NEB 2144S, Ipswich, MA, USA); H3K9me3 1:200 (Active Motif 61013,

(10) Axonemal Growth and Alignment During Paraspermatogenesis in the Marine Gastropod Strombus luhuanusFrontiers in Cell and Developmental BiologyJune 27, 2022Daisuke Shibata, Masaya Morita, Yu Sato, Kogiku Shiba, Seiya Kitanobo, Ryo Yokoya, Kazuo Inabathe method of Satouh and Inaba (2009). Other antibodies used in this study included anti-γ-tubulin (ab11316, Abcam, Tokyo, Japan; T6557, Sigma-Aldrich, St. Louis, MO, United States), anti-acetylated α-tubulin

(11) Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacementiScienceJune 17, 2022Fdez E, Madero-Pérez J, Lara Ordóñez AJ, Naaldijk Y, Fasiczka R, Aiastui A, Ruiz-Martínez J, López de Munain A, Cowley SA, Wade-Martins R, et al.mouse monoclonal anti-pericentrin (Abcam, ab28144, 1:1000), mouse monoclonal anti-γ-tubulin (Abcam, ab11316, 1:1000), mouse monoclonal anti-FLAG (Sigma, clone M2, F1804, 1:1000), mouse monoclonal anti-c-myc (Sigma,

(12) Factor quinolinone inhibitors disrupt spindles and multiple LSF (TFCP2)-protein interactions in mitosis, including with microtubule-associated proteinsPLOS ONEJune 15, 2022Sarah A. Yunes et al.anti-α-tubulin antibody (1:500, Thermo Fisher 62204), mouse monoclonal anti-γ-tubulin (1:1000, Abcam ab11316), rabbit polyclonal anti-α-tubulin (1:5000, Thermo Fisher PA529444), and anti-α-tubulin antibody (Abcam

(13) An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodelingCell stem cellJune 2, 2022Kann AP, Hung M, Wang W, Nguyen J, Gilbert PM, Wu Z, Krauss RS.RRID:AB_305329 Rabbit anti-α-tubulin,GeneTex,Cat# GTX628802; RRID:AB_2716636 Mouse anti-γ-tubulin,Abcam,Cat# ab11316; RRID:AB_297920 Mouse anti-MYOD,BD Biosciences,Cat# 554130; RRID:AB_395255 Rabbit anti-Ddx6,Bethyl Laboratories

(14) NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modesNucleic acids researchMay 20, 2022Bao H, Carraro M, Flury V, Liu Y, Luo M, Chen L, Groth A, Huang H.IF",Abcam,ab181169 HAT1,1:1000,Abcam,ab194296 ASF1,1:1000,"Groth et al., 2007",N/A tubulin,1:10000,Abcam,ab11316 actin,1:10000,Sigma,A5316 HA,1:1000,Cell signaling,3724 myc,1:1000,Millipore,05–724 Antibodies

(15) Ependyma: a new target for autoantibodies in neuromyelitis optica?Brain communicationsJanuary 1, 2022Bigotte M, Gimenez M, Gavoille A, Deligiannopoulou A, El Hajj A, Croze S, Goumaidi A, Malleret G, Salin P, Giraudon P, et al.Laboratories 610062, FoxJ1,Mouse,1/500,eBioscience (Fisher) 14-9965-82, γ-tubulin,Mouse,1/500,Abcam ab11316, Ki67,Rabbit,1/500,Abcam ab15580, Secondary antibodies,Secondary antibodies,Secondary antibodies,Secondary

(16) NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activationCellDecember 22, 2021Andreeva L, David L, Rawson S, Shen C, Pasricha T, Pelegrin P, Wu H.(1:1000, Cell Signaling, Cat. no: 67824) and mouse monoclonal anti-γ-tubulin (1:500, Abcam, Cat. no: ab11316). After incubation, cells were washed and incubated with AlexaFluor647-conjugated anti-rabbit IgG (1:500,

(17) Maternal Smc3 protects the integrity of the zygotic genome through DNA replication and mitosisDevelopmentDecember 15, 2021Wei-Ting Yueh, Vijay Pratap Singh, Jennifer L. Gertonsaline supplemented with 0.01% Tween (TBSTw) for 1 h. SMC3 (Abcam; ab9263; 1:2000) and γ-tubulin (Abcam; ab11316; 1:1000) primary antibodies were diluted in 0.3% blocking buffer at 4°C overnight. Blots were washed

(18) Factor quinolinone inhibitors alter cell morphology and motility by destabilizing interphase microtubulesScientific reportsDecember 7, 2021Stoiber P, Scribani Rossi P, Pokharel N, Germany JL, York EA, Schaus SE, Hansen U.described above, blocked in 5% BSA in PBS and stained overnight at 4 °C with anti-γ-tubulin (1:500, Abcam, ab11316) and anti-α-tubulin (1:500, Fisher Scientific, 62204). Samples were then incubated in goat anti-rabbit

(19) Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functionsThe Journal of cell biologyDecember 6, 2021Kumari A, Kumar C, Pergu R, Kumar M, Mahale SP, Wasnik N, Mylavarapu SVS.cyclin B1 (Abcam; ab32053), GM130 (Abcam; ab 52649), LAMP1 (Sigma-Aldrich; L1418), γ-tubulin (Abcam; ab11316), mCherry (Abcam; ab167453), β-COP (Abcam; ab2899), BICD2 (Abcam; ab117818), p50 (BD Biosciences; 611002),

(20) A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defectsJournal of human geneticsDecember 1, 2021Markus F, Kannengießer A, Näder P, Atigbire P, Scholten A, Vössing C, Bültmann E, Korenke GC, Owczarek-Lipska M, Neidhardt J.4% PFA for 30 min. Blocking and ICC was performed using following antibodies: anti-γ-tubulin (1:500, ab11316, Abcam, Cambridge, UK), anti-detyrosinated α-tubulin (1:1000, D-tubulin, ab3201, Merck Millipore), Alexa

Write a Review

(1)

3/16/2017Great Gamma-Tubulin Antibody Brian ORourkeThis mouse monoclonal antibody is ideal for immunofluorescence imaging of methanol fixed cells. This widely published antibody will allow crisp staining of the cells at low dilution.
(1)

9/16/2016Great Gamma Tubulin Antibody Lilian KabecheI used this antibody to stain for centrosomes. The antibody worked great at 1:1000 for 1 hour at room temp. Here is the full protocol I used: Cells were fixed in 3.5% paraformaldehyde for 15 minutes ... Read Review

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker from Abcam

Abcam

Citations (137)

Reviews

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map