Anti-Metallothionein antibody [UC1MT] ab12228 from Abcam

Product Specs
ItemAnti-Metallothionein antibody [UC1MT]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab12228
Antibody TypePrimary
CloneUC1MT
IsotypeIgG1
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot
HostMouse, Nude mouse
Quantity100 UG
ClonalityMonoclonal
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameMT1A
ReactivityHuman, Rabbit
TargetMetallothionein

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Metallothionein synthesis increased by Ninjin-yoei-to, a Kampo medicine protects neuronal death and memory loss after exposure to amyloid Î²1-42J Pharm Health Care SciNovember 1, 2022Haruna Tamano, Haruna Tokoro, Daichi Murakami, Rin Tsujimoto, Yuka Nishijima, Erina Tsuda, Satoshi Watanabe, Miki Suzuki, Atsushi Takedafor 1 h, and rinsed with PBS for 5 min followed by overnight incubation with anti-MT antibody [UC1MT] ab12228 (Abcam) in 0.1% Triton X-100 in PBS (1:200 dilution) at 4 °C. The slides were rinsed with PBS for 5

(2) Resolving the immune landscape of human prostate at a single-cell level in health and cancerCell reportsDecember 21, 2021Tuong ZK, Loudon KW, Berry B, Richoz N, Jones J, Tan X, Nguyen Q, George A, Hori S, Field S, et al.AB_445401 anti-human CD206 (PE/Dazzle),BioLegend,Cat#321130; clone 15-2; RRID: AB_2616867 anti-human MT1,Abcam,Cat#ab12228; clone UC1MT; RRID: AB_298949 anti-human CD3 (AF488),BioLegend,Cat#300415; clone UCHT1; RRID: AB_389310 anti-human

(3) Antitumor effects of cadmium against diethylnitrosamine‑induced liver tumors in miceOncology LettersNovember 26, 2021Yu Nie, Bo Huang, An-Ling Hu, Yun-Yan Xu, Yan Zou, Yun Liu, Jie Liu1 h at room temperature. Subsequently, sections were incubated with MT primary antibodies (cat. no. ab12228, 1:100; Abcam) overnight at 4°C. After washing with PBS for 5 min, slides were incubated with HRP-conjugated

(4) Preventive effect of Ninjin-yoei-to, a Kampo medicine, on amyloid β_1-42-induced neurodegeneration via intracellular Zn²⁺ toxicity in the dentate gyrusExperimental AnimalsNovember 10, 2021Haruna Tamano et al.and rinsed with PBS for 5 min followed by overnight incubation with anti-MT 224 antibody [UC1MT] ab12228 (Abcam) in 0.1% Triton X-100 in PBS at 4°C. The slides 225 were rinsed with PBS for 5 min three times

(5) BCRP/ABCG2 Transporter Regulates Accumulation of Cadmium in Kidney Cells: Role of the Q141K Variant in Modulating NephrotoxicityDrug Metabolism and DispositionAugust 1, 2021Xia Wen, Danielle Kozlosky, Ranran Zhang, Cathleen Doherty, Brian Buckley, Emily Barrett, Lauren M. Aleksunes(ALX-801-036C100), HO-1 (ADI-SPA-895-F) (Enzo Life Sciences, Farmingdale, NY), NQO1 (ab80588), MT1/2 (ab12228), SOD-1 (ab13498), or TRXR (ab18840) (Abcam, Cambridge, MA) followed Downloaded from dmd.aspetjournals.org

(6) Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healingCell stem cellJuly 1, 2021Altshuler A, Amitai-Lange A, Tarazi N, Dey S, Strinkovsky L, Hadad-Porat S, Bhattacharya S, Nasser W, Imeri J, Ben-David G, et al.Polyclonal anti-Atf3,Santa Cruz,Cat#AB_225851 Mouse monoclonal anti-Mt1-2(Methallothionein),Abcam,Cat#ab12228; AB_298949 Rabbit monoclonal anti-Krt12,Abcam,Cat#ab185627 Mouse monoclonal anti-Krt15,Santa Cruz,Cat#sc-47697;

(7) Effect of MT2A on apoptosis and proliferation in HL60 cellsInternational Journal of Medical SciencesJune 4, 2021Yu-Qing Pan et al.antibodies were as follows: anti-human MT2A/IκB-α mouse monoclonal antibody(diluted 1:2000, Cat.No. ab12228/ab12134, Abcam, Cambridge, UK), anti-human Bax/Bcl2 mouse monoclonal antibody (diluted 1:500, Cat.No.

(8) Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinomaTheranosticsJanuary 1, 2021Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, Niu Y, Xu X, Guo S, Yang Y, et al.#ab207327), anti-hemoglobin subunit alpha 1 (HBA1, Abcam, #ab77125), anti-metallothionein 2A (MT2A, Abcam, #ab12228), anti-keratin 81 (KRT81, Abcam, #ab55407), anti-hemoglobin subunit delta (HBD, Abcam, #ab131225), anti-carbonic anhydrase 12 (CA12

(9) Single-nucleus RNA-seq identifies Huntington disease astrocyte statesActa Neuropathologica CommunicationsDecember 1, 2020Osama Al-Dalahmah, Alexander A. Sosunov, A. Shaik, Kenneth Ofori, Yang Liu, Jean Paul Vonsattel, Istvan Adorjan, Vilas Menon, James E. GoldmanC3 (rabbit monoclonal, 1:200, Abcam Ab20999), metallothionein (MT) (mouse monoclonal, 1:200, Abcam ab12228), HTT (mouse monoclonal, 1:2000 Millipore MAB5492 1:2000), ALDH1L1 (mouse monoclonal, Encor Cat# MCA-2E7,

(10) D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degenerationSignal transduction and targeted therapyMarch 4, 2020Wang B, Wang L, Gu S, Yu Y, Huang H, Mo K, Xu H, Zeng F, Xiao Y, Peng L, et al.#PA5–38294), F-actin (Phalloidin FITC, Yepsen Biotechnology, #40735ES75), Metallothionein (Abcam, #ab12228), HMGB1 (Abcam, #ab18256), LC3B (CST, #3868), 8-OHdG (Santa Cruz, #SC-393871), Alexa Fluor 488-labeled

(11) A bioengineered living cell construct activates metallothionein/zinc/MMP8 and inhibits TGF β to stimulate remodeling of fibrotic venous leg ulcersWound Repair and RegenerationMarch 1, 2020Rivka C. Stone, Olivera Stojadinovic, Andrew P. Sawaya, George D. Glinos, Linsey E. Lindley, Irena Pastar, Evangelos Badiavas, Marjana Tomic‐CanicBiosystems) in PBS/ 0.1% Tween-20, and probed with antimetallothionein (Abcam, San Francisco, CA; Cat. No. ab12228), anti-MMP8 (Abcam Cat. No. ab81286), or antidecorin (Santa Cruz Biotechnology; Cat. No. sc-22,753).

(12) C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarctionCell death & diseaseJuly 11, 2019Zhang Z, Zhu L, Feng P, Tan Y, Zhang B, Gao E, Wang X, Fan C, Wang X, Yi W, et al.Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-MMP9 (cat. no. ab38898; Abcam), anti-MT1/2 (cat. no. ab12228; Abcam), anti-SOD2 (cat. no. 13141; Cell Signaling Technology), anti-phospho-AKT (cat. no. 4058; Cell Signaling Technology

(13) Zinc Inhibits HIF-Prolyl Hydroxylase Inhibitor-Aggravated VSMC Calcification Induced by High PhosphateFrontiers in physiologyJanuary 1, 2019Nagy A, Pethő D, Gáll T, Zavaczki E, Nyitrai M, Posta J, Zarjou A, Agarwal A, Balla G, Balla J.States, Cat.: bs-5685R) at 1:500 dilution, metallothionein (Abcam, Cambridge, United Kingdom; Cat.: # ab12228) 1:1000, and PDK-4 (Abcam, Cat.: ab88063) at 1:1000 dilution. Antigen-antibody complexes were visualized

(14) The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial CellsNutrientsDecember 2, 2018Sara Rodríguez-Menéndez, Montserrat García, Beatriz Fernández, Lydia Álvarez, Andrés Fernández-Vega-Cueto, Miguel Coca-Prados, Rosario Pereiro, Héctor González-Iglesiasgoat serum (10% in PBS), and further incubated overnight with a mouse monoclonal antibody to MT1/2 (ab12228, Abcam; dilution 1:100), at 4 °C. Then, cells were washed with PBS (3 times, 5 min) and incubated at

(15) Magnesium isoglycyrrhizinate has hepatoprotective effects in an oxaliplatin‑induced model of liver injuryInternational journal of molecular medicineOctober 1, 2018Zou X, Wang Y, Peng C, Wang B, Niu Z, Li Z, Niu J.primary antibodies at 4°C overnight. The following antibodies were used: Metallothionein 1 (cat. no. ab12228; 1:2,000; Abcam, Cambridge, MA, USA), peroxiredoxin 1 (cat. no. 8499; 1:5,000; Cell Signaling Technology,

(16) Metallothionein expression in slow- vs. fast-twitch muscle fibers following 4 weeks of streptozotocin-induced type 1 diabetesFACETSOctober 1, 2018Pamela Mondragon, Andreas Bergdahlwash buffer. Then the sections were incubated overnight at 4 °C with the primary MT-I+II antibody (ab12228; Abcam, Toronto, Ontario, Canada) diluted in PBS with 1% BSA 1:500 followed by horseradish peroxidase

(17) MT1G is Silenced by DNA Methylation and Contributes to the Pathogenesis of Hepatocellular CarcinomaJournal of CancerJuly 16, 2018Ju-deng Zeng, Ning Zhang, Gui-jun Zhao, Li-xia Xu, Yang Yang, Xiao-yi Xu, Meng-ke Chen, Hui-yun Wang, Steven Xiao-feng Zheng, Xiao-xing Lisites on membrane were sealed with 5% milk, and then incubated with a primary antibody (UC1MT; Abcam, ab12228) and secondary antibody. The signals were detected using the enhanced chemiluminescence method. Colony

(18) Decreased long non-coding RNA MTM contributes to gastric cancer cell migration and invasion via modulating MT1FOncotargetNovember 14, 2017Zhenghua Lin, Sanchuan Lai, Xingkang He, Wei Zhuo, Lan Wang, Jianmin Si, Shujie Chen

(19) ASSOCIATION OF CD44+CD24−/low WITH MARKERS OF AGGRESSIVENESS AND PLASTICITY OF CELL LINES AND TUMORS OF PATIENTS WITH BREAST CANCERExperimental OncologySeptember 22, 2017V F Chekhun, N Yu Lukianova, S V Chekhun, N O Bezdieniezhnykh, T V Zadvorniy, T V Borikun, L Z Polishchuk, O M Klyusovanti-metallothionein Experimental Oncology 39, �� 203–211, �� (September) 205 (clone ab12228), anti-glutathione-S-transferase (clone ab18183), anti-ferroportin (clone ab78066; Abcam) (Abcam, USA);

(20) Metallothioneins regulate the adipogenic differentiation of 3T3-L1 cells via the insulin signaling pathwayPloS oneJanuary 1, 2017Kadota Y, Toriuchi Y, Aki Y, Mizuno Y, Kawakami T, Nakaya T, Sato M, Suzuki S.

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Anti-Metallothionein antibody [UC1MT] from Abcam

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Anti-Metallothionein antibody [UC1MT] from Abcam

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