Anti-MiTF antibody [C5] ab12039 from Abcam

Product Specs
ItemAnti-MiTF antibody [C5]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab12039
Antibody TypePrimary
CloneC5
HostMouse, Nude mouse
IsotypeIgG1
ClonalityMonoclonal
ApplicationsEMSA, Flow Cytometry, Western blot
Quantity50 UG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameMITF
ReactivityHuman
TargetMiTF

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-κB- and PRMT5-Mediated PGC1α ReductionCancersJuly 4, 2023Tamiya H, Urushihara N, Shizuma K, Ogawa H, Nakai S, Wakamatsu T, Takenaka S, Kakunaga S.Santa Cruz Biotechnology), SDMA (SYM10; 07-412, Millipore), SOX10 (sc-365692, Santa Cruz Biotechnology), MITF (ab12039, Abcam), LC3B (NB100-2220, Novus Biologicals), NRF2 (#12721, Cell Signaling Technology), Parkin (#4211,

(2) Dedifferentiation maintains melanocyte stem cells in a dynamic nicheNatureApril 27, 2023Qi Sun, Wendy Lee, Hai Hu, Tatsuya Ogawa, Sophie De Leon, Ioanna Katehis, Chae Ho Lim, Makoto Takeo, Michael Cammer, M. Mark Taketo, et al.Abcam, ab15580); mouse anti-β-catenin (1:400; Sigma-Aldrich, C7207); and mouse anti-MITF (1:100; Abcam, ab12039). The following secondary antibodies from Thermo Fisher were used: Alexa Fluor 594 donkey anti-mouse

(3) An adverse tumor-protective effect of IDO1 inhibitionCell Reports MedicineFebruary 21, 2023Juliana C.N. Kenski, Xinyao Huang, David W. Vredevoogd, Beaunelle de Bruijn, Joleen J.H. Traets, Sofía Ibáñez-Molero, Sebastiaan M. Schieven, Alex van Vliet, Oscar Krijgsman, Thomas Kuilman, et al.following antibodies were used: pSTAT1 (9167), IDO1 (86630), ATF4 (11815), from Cell Signaling; MITF (ab12039) from Abcam, Vinculin (V9131) from Sigma, HSP90 alpha/beta (H-114) from Santa Cruz. RNA-sequencing

(4) Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunctionTheranosticsJanuary 1, 2023Park YJ, Kim JC, Kim Y, Kim YH, Park SS, Muther C, Tessier A, Lee G, Gendronneau G, Forestier S, et al.transferred to a polyvinylidene fluoride membrane (Millipore, Burlington, MA). The antibodies against MITF (ab12039) and RAB27 (ab55667) were obtained from Abcam (Cambridge, United Kingdom); Tyrosinase (sc7833) was from

(5) Epigenetic and pharmacological control of pigmentation via Bromodomain Protein 9 ( BRD9 )Pigment Cell & Melanoma ResearchJanuary 1, 2023Tupa Basuroy, Megan Dreier, Caitlin Baum, Thomas Blomquist, Robert Trumbly, Fabian V. Filipp, Ivana L. de la Sernaantibodies. (Keenen et al., 2010). The BRD9 antibody (A303781) was from Bethyl Laboratories; the MITF (ab12039) and SMARCA4 (ab110641) antibodies were from Abcam. SOX10 (N20), TYR (sc‐7833), and TYRP1 (sc‐10,443)

(6) MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesisJournal of Biological ChemistryDecember 1, 2022Jyoti Tanwar, Akshay Sharma, Suman Saurav, Shyamveer, Nidhi Jatana, Rajender K. Motianinumber DCT,Abcam,ab74073 Gp100,Abcam,ab137078 β-Tubulin,Abcam,ab21058 STIM1,Abcam,ab108994 MITF,Abcam,ab12039 Calnexin,Abcam,ab22595 Orai1,Abcam,ab86748 Orai2,Abcam,ab180146 Orai3,Abcam,ab254260 Details

(7) Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITFCellsNovember 12, 2022Wang H, Zhang H, Chen Y, Wang H, Tian Y, Yi X, Shi Q, Zhao T, Zhang B, Gao T, et al.Massachusetts, USA, 1:1000 for WB), active β-catenin (cat. 8814, CST, 1:200 for IF, 1:1000 for WB), MITF (cat. ab12039, Abcam, Cambridge, UK, 1:1000 for WB), PGC1α (cat. 66369-1-Ig, Proteintech, Wuhan, China, 1:1000 for WB

(8) Sensitivity of the Dorsal-Central Retinal Pigment Epithelium to Sodium Iodate-Induced Damage Is Associated With Overlying M-Cone Photoreceptors in MiceInvestigative Opthalmology & Visual ScienceAugust 26, 2022Lili Lian et al.and incubated overnight with primary antibodies at 4°C. Primary antibodies were anti-MITF (1:1000, ab12039; Abcam, Cambridge, MA, USA) and anti-GAPDH (KC-5GC, 1:5000; Aksomics, Shanghai, China). After washing

(9) Persister state-directed transitioning and vulnerability in melanomaNature communicationsJune 1, 2022Chauvistré H, Shannan B, Daignault-Mill SM, Ju RJ, Picard D, Egetemaier S, Váraljai R, Gibhardt CS, Sechi A, Kaschani F, et al.a DAKO autostainer according to the manufacturer’s protocol using the same antibodies or anti-MITF ab12039 (Abcam, Cambridge, UK, 1:1000). For immunofluorescence staining of KDM5B, cultured cells were fixed

(10) Temporal analysis of melanogenesis identifies fatty acid metabolism as key skin pigment regulatorPLOS BiologyMay 18, 2022Farina Sultan et al.separated on 10% SDS PAGE gel. Tyrosinase antibody is synthesized from Genescript. PMEL17 (ab137078), MITF (ab12039), and FASN (ab22759) antibodies are procured from Abcam, while SREBF1 (04–469) is ordered from Millipore.

(11) Vitamin E enriched diet increases the rate of orthodontic tooth movementAmerican Journal of Orthodontics and Dentofacial OrthopedicsMay 1, 2022Christina Seong, Po-Jung Chen, Zana Kalajzic, Shivam Mehta, Ambika Sharma, Ravindra Nanda, Sumit Yadav, Eliane H. Dutrainstructions. In addition, immunofluorescence for microphthalmia-associated transcription factor (MITF) (ab12039, Abcam, Cambridge, United Kingdom) and ligand receptor activator of nuclear factor kappa B (RANKL) (LS-B1425;

(12) CD44+ and CD133+ Non-Small Cell Lung Cancer Cells Exhibit DNA Damage Response Pathways and Dormant Polyploid Giant Cancer Cell Enrichment Relating to Their p53 StatusInternational Journal of Molecular SciencesApril 28, 2022Margarita Pustovalova, Taisia Blokhina, Lina Alhaddad, Anna Chigasova, Roman Chuprov-Netochin, Alexander Veviorskiy, Gleb Filkov, Andreyan N. Osipov, Sergey Leonovab72182, Abcam, Cambridge, MA, USA), mouse monoclonal Anti-MiTF antibody [C5] (dilution 1:100, Cat. # ab12039, Abcam, Cambridge, MA, USA) and secondary goat anti-rabbit Alexa Fluor 488 conjugated (dilution 1:500;

(13) Preferential stimulation of melanocytes by M2 macrophages to produce melanin through vascular endothelial growth factorScientific reportsApril 19, 2022Han H, Kim Y, Mo H, Choi SH, Lee K, Rim YA, Ju JH.containing 0.1% Tween 20 (PBST) and 3% BSA for 1 h and then incubated with an anti-MITF antibody (1:1000, ab12039; Abcam), anti-tyrosinase antibody (1:500, sc-20035; Santa Cruz Biotechnology, Dallas, TX, USA), and

(14) Flavonoids increase melanin production and reduce proliferation, migration and invasion of melanoma cells by blocking endolysosomal/melanosomal TPC2Scientific ReportsDecember 1, 2021Ponsawan Netcharoensirisuk et al.cat. #Sc-71588), MITF (Cell Signaling Technology, 1:1000, cat. #97800), MITF (Abcam, 1:1000, cat. #ab12039), GSK-3β (Cell Signaling Technology, 1:1000, cat. #9832), CREB and pCREB (Cell Signaling Technology,

(15) BRN2 is a non-canonical melanoma tumor-suppressorNature CommunicationsDecember 1, 2021Michael Hamm et al.for 1.5 h at RT and then probed with antibodies against BRN2 (Cell signaling, #12137), MITF (Abcam, ab12039), β-actin (Sigma, A5441), cyclin D1 (Cell signaling, #2926), PTEN (Cell signaling, #9559), phospho-S6

(16) The feather pattern autosomal barring in chicken is strongly associated with segregation at the MC1R locusPigment Cell & Melanoma ResearchNovember 1, 2021Doreen Schwochow, Susanne BornelÃ¶v, Tingxing Jiang, Jingyi Li, David Gourichon, Bertrand Bedâ€™Hom, Ben J. Dorshorst, Chengâ€Ming Chuong, MichÃ¨le Tixierâ€Boichard, Leif Anderssonwere processed for immunohistochemistry or in situ hybridization. The MITF antibody was from Abcam (ab12039, 1:200 dilution). The peroxidase staining was used after primary antibody treatment as described (Jiang

(17) A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cellsLab on a ChipOctober 24, 2021Brenda L. K. Coles, Mahmoud Labib, Mahla Poudineh, Brendan T. Innes, Justin Belair-Hickey, Surath Gomis, Zongjie Wang, Gary D. Bader, Edward H. Sargent, Shana O. Kelley, et al.RRID:AB_477010) from MilliporeSigma; PKCα (MA1-157, RRID:AB_2536865) from Thermo Fisher Scientific; MiTF (ab12039, RRID:AB_298801) and CRX (ab193448) from Abcam; Chx10 (sc-365519, RRID:AB_10842442) from Santa Cruz Biotechnology

(18) Cajanin Suppresses Melanin Synthesis through Modulating MITF in Human Melanin-Producing CellsMoleculesOctober 5, 2021Ponsawan Netcharoensirisuk, Kaoru Umehara, Wanchai De-Eknamkul, Chatchai ChaothamBiotech Ltd. (New Taipei City, Taiwan). Antibodies, including mouse monoclonal antibody to MITF (Cat No. ab12039), mouse monoclonal antibody to TRP-1 (Cat No. ab3312), rabbit polyclonal antibody to Dct (Cat No. ab74073)

(19) Activation of the integrated stress response confers vulnerability to mitoribosome-targeting antibiotics in melanomaJournal of Experimental MedicineSeptember 6, 2021Roberto Vendramin et al.hVIN-1; Sigma-Aldrich, 1:5,000), histone 3 (#4499, clone D1H2; Cell Signaling Technology, 1:1,000), MITF (ab12039; Abcam, 1:1,000), ATF4 (#11815, clone D4B8; Cell Signaling Technology, 1:1,000), ATF5 (SAB4500895; Sigma-Aldrich,

(20) Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytesThe British journal of dermatologyAugust 1, 2021Lan Y, Zeng W, Dong X, Lu H.antimouse IgG (A0428; Beyotime Biotechnology, Haimen, Jiangsu, China); anti‐MITF mouse monoclonal antibody (ab12039; Abcam) conjugated to Cy3‐labelled goat antimouse IgG (A0521; Beyotime); and anti‐OPN5 rabbit polyclonal

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Anti-MiTF antibody [C5] from Abcam

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Anti-MiTF antibody [C5] from Abcam

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