Fig 1: TUBB4A expression in normal prostate and prostate cancer tissues.Differential expression of TUBB4A A between normal prostate and prostate cancer tissues, B between tumor tissues with different Gleason scores, and C between AA and EA tumor tissues from TCGA dataset. Numbers in parentheses indicate the sample size. D DNA methylation of TUBB4A promoter (TCGA dataset). E Correlation of mRNA expression levels with DNA methylation levels in the CpG island of TUBB4A in prostate cancer samples (TCGA dataset). F Kaplan–Meier survival curves for TUBB4A expression with low and high levels in TCGA prostate cancer patients. G Representative IHC staining in normal prostate and prostate cancer tissues with anti-human TUBB4A mAb (Abcam, ab11315) from UAB prostate cancer specimens. Scale bars, 100 µm or 200 µm. This experiment was repeated two times. H–J Quantitative H-scores for prostate cancer samples from European and African American ancestry and tumor stages and Gleason scores. Data are presented as the medians and interquartile ranges as determined with a Kruskal–Wallis (B–D) or Mann–Whitney U (A–D) test. Data are presented as the means and standard deviation (SD) as determined with an ANOVA followed by Tukey’s post hoc t test (H–J). Source data are provided as a Source data file. AA: African-American; EA: European-American; NAT: Normal prostate tissue adjacent to the tumor.