Goat anti-V5 Tag Antibody Agarose Immobilized from Bethyl Laboratories, Inc.

Product Specs
ItemGoat anti-V5 Tag Antibody Agarose Immobilized
CompanyBethyl Laboratories, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS190-119
Quantity100 µg
ApplicationsIP
Conjugate/Tag/LabelAgarose Immobilized
FormatWhole IgG
TargetV5 Tag
IsotypeWhole IgG
HostGoat
Validated ApplicationsImmunoprecipitation (IP)
ClonalityPolyclonal
Storage2 - 8°C

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Bethyl Laboratories, Inc.

P.O. Box 850

Montgomery, Texas 77356

United States

Phone: 1-800-338-9579
Fax: 1-866-597-6105

Email: orders@fortislife.com

Company Profile

Website: fortislife.com

(1) Validation of commercially available antibodies directed against subunits of the epithelial Na(+) channelPhysiological reportsJanuary 1, 2023Mutchler SM, Shi S, Whelan SCM, Kleyman TR.Invitrogen), only 40 μl rec‐protein G‐Sepharose (negative control) or 40 μl V5‐beads (positive control, S190‐119, Bethyl) overnight at 4°C on an end‐to‐end incubator. After extensive washes with PBS, 60 μl Laemmli sample buffer

(2) SUSD2 suppresses CD8(+) T cell antitumor immunity by targeting IL-2 receptor signalingNature immunologyNovember 1, 2022Zhao B, Gong W, Ma A, Chen J, Velegraki M, Dong H, Liu Z, Wang L, Okimoto T, Jones DM, et al.with Protease Inhibitor Cocktail. Total protein extracts were incubated with goat anti-V5 agarose (S190-119; Bethyl Laboratories) or anti-Flag M2 Affinity Gel (A2220; Sigma-Aldrich) overnight at 4°C under gentle

(3) PLAC1 affects cell to cell communication by interacting with the desmosome complexPlacentaJuly 1, 2021Yaohui Chen, Carole Stagg, David Schlessinger, Ramaiah Nagarajaat 13,000 at 4C for 15 minutes and the supernatants were incubated with goat anti-V5 agarose beads (S190-119, Bethyl lab) at 4°C, overnight. The beads were washed X5 with IP buffer at 4C, 15 minutes each, on a

(4) Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategyNature MicrobiologyMarch 1, 2021Tianliang Li et al.for immunoprecipitation included Anti-FLAG M2 Affinity Gel (A2220) (Sigma-Aldrich), anti-V5 agarose (S190–119) (Bethyl Laboratories), and anti-acetyl-Lysine agarose (ICP0388) (ImmuneChem). Listeriolysin O (LLO)

(5) Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase ComplexCell ReportsMarch 14, 2017Martin Kucej, Charles S. Fermaintt, Kun Yang, Ricardo A. Irizarry-Caro, Nan Yan

(6) Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase ComplexCell ReportsMarch 14, 2017Martin Kucej, Charles S. Fermaintt, Kun Yang, Ricardo A. Irizarry-Caro, Nan Yan1:1,000 dilution, ab4-100, Abcam), anti-V5 agarose beads (goat, 2 μg per immunoprecipitation [IP], S190-119, Bethyl Laboratories), and anti-Myc (rabbit, 2 μg per IP, sc-789, Santa Cruz). Flow Cytometry and Fluorescence Microscopy

(7) Stabilizing the Hsp70-Tau Complex Promotes Turnover in Models of TauopathyCell chemical biologyAugust 18, 2016Young ZT, Rauch JN, Assimon VA, Jinwal UK, Ahn M, Li X, Dunyak BM, Ahmad A, Carlson GA, Srinivasan SR, et al.of lysate was incubated with 40 μL of goat anti-V5 conjugated to agarose beads (Bethyl Laboratories, S190-119) and 50 μM JG-48 or DMSO (5%) at 4 °C in the dark overnight. Lysate was incubated with normal goat IgG

(8) Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle DifferentiationPloS oneJanuary 1, 2016Ansseau E, Eidahl JO, Lancelot C, Tassin A, Matteotti C, Yip C, Liu J, Leroy B, Hubeau C, Gerbaux C, et al.products, the lysate was incubated with V5 antibody agarose-immobilized conjugate (Bethyl Laboratories, S190-119) or Myc antibody agarose immobilized conjugate (Millipore, 16–219) for 16–20 h at 4°C. Immunoprecipitated

(9) Malawi Polyomavirus Is a Prevalent Human Virus That Interacts with Known Tumor SuppressorsJournal of VirologyJanuary 1, 2015Christian Berrios et al.

(10) Tissue-specific splice variants of HARE/Stabilin-2 are expressed in bone marrow, lymph node, and spleenBiochemical and Biophysical Research CommunicationsNovember 5, 2014Amanda K. Hare, Edward N. Harris

(11) Prostasin interacts with the epithelial Na+ channel and facilitates cleavage of the γ-subunit by a second proteaseAmerican journal of physiology. Renal physiologyNovember 1, 2014Carattino MD, Mueller GM, Palmer LG, Frindt G, Rued AC, Hughey RP, Kleyman TR.

(12) Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAsNucleic acids researchJanuary 1, 2013Färber V, Erben E, Sharma S, Stoecklin G, Clayton C.

(13) Analysis of the Tau-Associated Proteome Reveals That Exchange of Hsp70 for Hsp90 Is Involved in Tau DegradationACS Chemical BiologyOctober 19, 2012Andrea D. Thompson, K. Matthew Scaglione, John Prensner, Anne T. Gillies, Arul Chinnaiyan, Henry L. Paulson, Umesh K. Jinwal, Chad A. Dickey, Jason E. Gestwickiimmunoprecipitated by incubating with 75 μl of goat anti-V5 conjugated to agarose beads (Bethyl Laboratories, S190-119) at 4 °C overnight in the dark. Beads were subsequently washed with 100 μL of PBS (10mM Na2HPO4, 2 mM

(14) Probing structural selectivity of synthetic heparin binding to Stabilin protein receptorsThe Journal of biological chemistryJune 15, 2012Pempe EH, Xu Y, Gopalakrishnan S, Liu J, Harris EN.

(15) FinTRIMs, fish virus-inducible proteins with E3 ubiquitin ligase activityDevelopmental and comparative immunologyFebruary 1, 2012van der Aa LM, Jouneau L, Laplantine E, Bouchez O, Van Kemenade L, Boudinot P.

(16) Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitizationThe Journal of biological chemistryApril 29, 2011Fuhs SR, Insel PA.

(17) The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular functionThe Journal of biological chemistryFebruary 4, 2011Ikeda M, Andoo A, Shimono M, Takamatsu N, Taki A, Muta K, Matsushita W, Uechi T, Matsuzaki T, Kenmochi N, et al.

(18) Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathwayCell death and differentiationAugust 1, 2009Park HA, Khanna S, Rink C, Gnyawali S, Roy S, Sen CK.

(19) Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growthEukaryotic cellAugust 1, 2009Bratschi MW, Burrowes DP, Kulaga A, Cheung JF, Alvarez AL, Kearley J, Zaremberg V.

(20) Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domainsGlycobiologyApril 1, 2008Chigira Y, Oka T, Okajima T, Jigami Y.