SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein from Sino Biological, Inc.

Product Specs
ItemSARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog Number40592-V08H82
FormatLyophilized from sterile PBS, pH 7.4
Conjugate/Tag/LabelC-His
TypeRecombinant protein
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions
Molecule NameSpike
Activity1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His(40592-V08H82), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His (40592-V08H82) is 3-20 ng/mL. 2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein(Cat. No. 40592-V08H82) with an affinity constant of 0.827 nM as determined by Octet RED96e system
Purity> 95 % as determined by SDS-PAGE
SpeciesSARS-CoV-2

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Sino Biological, Inc.

1400 Liberty Ridge Drive

Suite 101

Wayne, PA 19087

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: http://www.sinobiological.com/

(1) Physicochemical Nature of SARS-CoV-2 Spike Protein Binding to Human VimentinACS Applied Materials & InterfacesJuly 19, 2023Piotr Deptuła, Krzysztof Fiedoruk, Monika Wasilewska, Łukasz Suprewicz, Mateusz Cieśluk, Paulina Żeliszewska, Magdalena Oćwieja, Zbigniew Adamczyk, Katarzyna Pogoda, Robert BuckiP.1 RBD (#40592-V08H86), South Africa B.1.135 RBD (#40592-V08H85), and United Kingdom B.1.1.7 RBD (#40592-V08H82). Recombinant spike RBD proteins were aliquoted at 0.1 mg/mL in phosphate-buffered saline (PBS) (Gibco,

(2) Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccinationNature communicationsJuly 13, 2023Tong X, McNamara RP, Avendaño MJ, Serrano EF, García-Salum T, Pardo-Roa C, Bertera HL, Chicz TM, Levican J, Poblete E, et al.,Sino Biological,40592-V08H SARS-CoV-2 Alpha Variant S,Sino Biological,40589-V08B6 SARS-CoV-2 Alpha Variant RBD,Sino Biological,40592-V08H82 SARS-CoV-2 Beta Variant S,Sino Biological,40589-V08B7 SARS-CoV-2 Beta Variant RBD,Sino Biological,40592-V08H59 SARS-CoV-2

(3) Adsorption of SARS-CoV-2 Spike (N501Y) RBD to Human Angiotensin-Converting Enzyme 2 at a Lipid/Water InterfaceThe journal of physical chemistry. BMay 25, 2023Rozak H, Nihonyanagi S, Myalitsin A, Roy S, Ahmed M, Tahara T, Rzeznicka II.10108-H08H) and human spike (N501Y) region binding domain (spike (N501Y) RBD; MW = 26.6 kDa) (Cat. No. 40592-V08H82) were purchased from Sino Biological Inc. (Beijing, China) as lyophilized powders (lyophilized from

(4) An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variantsNature communicationsMay 23, 2023Tai W, Feng S, Chai B, Lu S, Zhao G, Chen D, Yu W, Ren L, Shi H, Lu J, et al.with SARS-CoV-2 RBD proteins (Sino Biological: wild-type, Cat. No# 40592-V08H; Alpha strain, Cat. No# 40592-V08H82; Beta strain, Cat. No# 40592-V08H84; Gamma strain, Cat. No# 40592-V08H86; Delta strain, Cat. No# 40592-V08H91;

(5) Comprehensive engineering of a therapeutic neutralizing antibody targeting SARS-CoV-2 spike protein to neutralize escape variantsmAbsDecember 31, 2022Taichi Kuramochi et al.N487R (SinoBiological, #40592-V08H75), F490S (SinoBiological, 40592-V08H41), N501 (SinoBiological, #40592-V08H82), and triple RBD mutation K417N, E484K, N501Y (Acrobiosystems, #SPD-C52Hp). Recombinant SARS-CoV-2 S

(6) Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2BiomedicinesDecember 17, 2022Kim JW, Min SW, Lee J, Shin HG, Choi HL, Yang HR, Lee JH, Cho YB, Shim H, Lee S.wild-type RBD histidine-tagged (RBD-His) (Cat#40592-V08B, Sino Biological), Alpha variant-RBD-His (Cat#40592-V08H82, Sino Biological), and Delta variant-RBD-His (Cat#40592-V08H90, Sino Biological) were pre-incubated

(7) Single-Nanoparticle-Based Digital SERS Sensing Platform for the Accurate Quantitative Detection of SARS-CoV-2ACS applied materials & interfacesAugust 31, 2022Shim JE, Kim YJ, Choe JH, Lee TG, You EA.(40150-V08B2), MERS-CoV S antigen (40069-V08B1), nucleocapsid (N) antigen (40068-V08B), and variant antigen (40592-V08H82, 40592-V08H84, and 40592-V08H91) were purchased from Sino Biological (China). Deionized (DI) water with

(8) Potent and broad neutralization of SARS-CoV-2 variants of concern (VOCs) including omicron sub-lineages BA.1 and BA.2 by biparatopic human VH domainsiScienceAugust 19, 2022Chen C, Saville JW, Marti MM, Schäfer A, Cheng MH, Mannar D, Zhu X, Berezuk AM, Banerjee A, Sobolewski MD, et al.paper,N/A SARS2 RBD S494P,Sino Biological,Cat# 40592-V08H18 SARS2 RBD N501Y,Sino Biological,Cat# 40592-V08H82 SARS2 RBD K417N/E484K/N501Y,Sino Biological,Cat# 40592-V08H85-B "SARS2 S1 K417N, E484K, and N501Y",Sino Biological

(9) Potent neutralizing anti-SARS-CoV-2 human antibodies cure infection with SARS-CoV-2 variants in hamster modeliScienceAugust 19, 2022Imbrechts M, Maes W, Ampofo L, Van den Berghe N, Calcoen B, Van Looveren D, Kerstens W, Rasulova M, Vercruysse T, Noppen S, et al.(REC31849-500, The Native Antigen Company), RBD(N439K)-His (40592-V08H14, Sino Biological), RBD(N501Y)-His (40592-V08H82, Sino Biological), RBD(E484K)-His (40592-V08H84, Sino Biological), RBD(Y453F)-His (40592-V08H80, Sino Biological

(10) SARS-CoV-2 mRNA vaccine induced higher antibody affinity and IgG titers against variants of concern in post-partum vs non-post-partum womenEBioMedicineMarch 1, 2022Lee Y, Grubbs G, Ramelli SC, Levine AR, Bathula A, Saharia K, Purcell M, Singireddy S, Dugan CL, Kirchoff L, et al.

(11) Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple IndividualsAnalytical ChemistryFebruary 8, 2022Lei Mou, Yingying Zhang, Yao Feng, Honghai Hong, Yong Xia, Xingyu Jiangprotein (40592-V08H) and their variants (India variant B.1.617: 40592-V08H88; U.K. variant B.1.1.7: 40592-V08H82; South Africa variant B.1.351: 40592-V08H84; Brazil variant P.1: 40591-V08H5) are from Sino Biological

(12) Nucleic acid delivery of immune-focused SARS-CoV-2 nanoparticles drives rapid and potent immunogenicity capable of single-dose protectionCell reportsFebruary 1, 2022Konrath KM, Liaw K, Wu Y, Zhu X, Walker SN, Xu Z, Schultheis K, Chokkalingam N, Chawla H, Du J, et al.Biological,40592-V08 "Recombinant RBD (K417T, E484K, N501Y)",Sino Biological,40592-V08H86 Recombinant RBD (N501Y),Sino Biological,40592-V08H82 "Recombinant RBD (K417N, E484K, N501Y)",Sino Biological,40592-V08H85 "Recombinant RBD (L452R, E484Q)",Sino Biological

(13) Rapid test to assess the escape of SARS-CoV-2 variants of concernScience advancesDecember 3, 2021Heggestad JT, Britton RJ, Kinnamon DS, Wall SA, Joh DY, Hucknall AM, Olson LB, Anderson JG, Mazur A, Wolfe CR, et al.RBD proteins for WT (Sino Biological, catalog no. 40592-V08H), B.1.1.7 (Sino Biological, catalog no. 40592-V08H82), P.1 (Sino Biological, catalog no. 40592-V08H86), and B.1.351 (Sino Biological, catalog no. 40592-V08H85)

(14) Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sexEBioMedicineDecember 1, 2021Tang J, Grubbs G, Lee Y, Huang C, Ravichandran S, Forgacs D, Golding H, Ross TM, Khurana S.

(15) Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cellsScientific reportsNovember 12, 2021Caohuy H, Eidelman O, Chen T, Liu S, Yang Q, Bera A, Walton NI, Wang TT, Pollard HB.protein (Cat # 40591-V08H3), Mink [Y453F] RBD protein (Cat # 40592-V08H80), (α/β/γ) [N501Y] RBD (Cat #40592-V08H82) and (β) (E484K) RBD proteins (Cat # 40592-Vo8H84) were purchased from SinoBiologicals U.S. Inc. (Chesterbrook,

(16) In vivo characterization of emerging SARS-CoV-2 variant infectivity and human antibody escape potentialCell reportsOctober 19, 2021Lam B, Kung YJ, Lin J, Tseng SH, Tsai YC, He L, Castiglione G, Egbert E, Duh EJ, Bloch EM, et al.proteins" ,, RBD-Fc,This paper,N/A RBD-His,This paper,N/A N501Y-RBD-His,Sino Biological,Catalog# 40592-V08H82 N501Y-RBD-Fc,This paper,N/A MPL,Sigma Aldrich,SAB1406140 D-luciferin,GoldBio,Catalog # eLUCNA-100;

(17) Epitope diversity of SARS-CoV-2 hyperimmune intravenous human immunoglobulins and neutralization of variants of concerniScienceSeptember 24, 2021Tang J, Lee Y, Ravichandran S, Grubbs G, Huang C, Stauft CB, Wang T, Golding B, Golding H, Khurana S.

(18) The neutralization potency of anti-SARS-CoV-2 therapeutic human monoclonal antibodies is retained against viral variantsCell reportsSeptember 7, 2021Makdasi E, Zvi A, Alcalay R, Noy-Porat T, Peretz E, Mechaly A, Levy Y, Epstein E, Chitlaru T, Tennenhouse A, et al.

(19) A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptationCellAugust 1, 2021Lin Kang, Guijuan He, Amanda K. Sharp, Xiaofeng Wang, Anne M. Brown, Pawel Michalak, James Weger-Lucarelli(Wayne, PA) using purified RBD from WT (Cat: 40592-V08H), A372T (Cat: 40592-V08H36), and N501Y (Cat: 40592-V08H82). RBDs were expressed in HEK293 cells and purified using the polyhistidine tag at the C terminus. Purity

(20) A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomesNature communicationsMay 11, 2021Guo Y, Huang L, Zhang G, Yao Y, Zhou H, Shen S, Shen B, Li B, Li X, Zhang Q, et al.#40592-V08H41), RBD (S494P, Sino Biological, Catalog #40592-V08H18), RBD (N501Y, Sino Biological, Catalog #40592-V08H82), or SARS-CoV-2 spike S1 domain (D614G, Sino Biological, Catalog #40591-V08H3) were injected through

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