8-hydroxy 2 deoxyguanosine ELISA Kit ab201734 from Abcam

Product Specs
Item8-hydroxy 2 deoxyguanosine ELISA Kit
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab201734
Quantity1 96TESTS
ApplicationsELISA
TypeCompetitive
Detection Range0.94 - 60 ng/ml
Sensitivity0.59 ng/ml
Detection Target8-hydroxy 2 deoxyguanosine

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(1) Tumor microenvironment in a minipig model of spinal cord gliomaJournal of Translational MedicineSeptember 27, 2023Muhibullah S. Tora, Stewart G. Neill, Yuliya Lakhina, Hemza Assed, Michelle Zhang, Purva P. Nagarajan, Thais Federici, Juanmarco Gutierrez, Kimberly B. Hoang, Yuhong Du, et al.minipig spinal cord glioma samples were harvested and quantified for 8-hydroxy 2 deoxyguanosine Kit (ab201734, ABCAM, USA), GSH/GSSG-Glo™ (Promega Corporation, USA) and Protein Carbonyl Assay Kit (#ab126287, ABCAM,

(2) Rapid phosphorylation of glucose-6-phosphate dehydrogenase by casein kinase 2 sustains redox homeostasis under ionizing radiationRedox BiologySeptember 1, 2023Yilong Hao, Tao Ren, Xiaoke Huang, Mi Li, Jong-Ho Lee, Qianming Chen, Rui Liu, Qingfeng TangThe level of 8-OHdG was examined by using 8-hydroxy 2 deoxyguanosine ELISA Kit obtained from Abcam (ab201734). The data was normalized to the cell numbers. 2.13 BrdU cell proliferation assay Cells were plated

(3) Biosynthesized Selenium Nanoparticles Using Epigallocatechin Gallate Protect against Pentylenetetrazole-Induced Acute Epileptic Seizures in Mice via Antioxidative, Anti-Inflammatory, and Anti-Apoptotic ActivitiesBiomedicinesJuly 11, 2023Barakat M. Alrashdi, Alaa Fehaid, Rami B. Kassab, Sara Rizk, Ola A. Habotta, Ahmed E. Abdel MoneimEllman’s method was used to analyze the reduced glutathione (GSH) levels [42]. The Abcam ELISA kit (ab201734) was employed to measure 8-hydroxy-2-deoxyguanosine (8OHdG) as an oxidative biomarker following the

(4) Association between micronutrients, oxidative stress biomarkers and angiogenic growth mediators in early and late-onset preeclamptic Ghanaian womenSAGE Open MedicineJune 6, 2023Enoch Odame Anto, Wina Ivy Ofori Boadu, Otchere Addai-Mensah, Yaw Amo Wiafe, William KBA Owiredu, Christian Obirikorang, Max Efui Annani-Akollor, Eric Adua, Michael Appiah, Stephen Opoku, et al.urinary and serum 8-OHdG were analysed in duplicates using highly sensitive and competitive ELISA kits (ab201734; Abcam, Shanghai, China). Serum concentrations were determined by comparison to a standard curve and

(5) Novel neuroendocrine role of γ-aminobutyric acid and gastrin-releasing peptide in the host response to influenza infectionMucosal immunologyJune 1, 2023Shirey KA, Lai W, Sunday ME, Cuttitta F, Blanco JCG, Vogel SN.Ontario, Canada). The 8-hydroxy 2 deoxyguanosine (8-OHdG) ELISA kit was purchased from Abcam (Catalog # ab201734; Branford, CT). GAD ELISA was purchased from Lifeome Biolabs (Catalog # EL009159MO-96; Oceanside, CA).

(6) Transcriptome Profiling Reveals Enhanced Mitochondrial Activity as a Cold Adaptive Strategy to Hypothermia in Zebrafish MuscleCellsMay 11, 2023Thomas Cahill, Sherine Chan, Ian M. Overton, Gary Hardiman1M Tris. The levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) were measured using the 8-OHdG ELISA kit (ab201734, Abcam) following manufacturer’s instructions. Absorbance values were measured using a CLARIOstar microplate

(7) Male reproductive traits are differentially affected by dietary macronutrient balance but unrelated to adiposityNature CommunicationsMay 4, 2023A. J. Crean, S. Afrin, H. Niranjan, T. J. Pulpitel, G. Ahmad, A. M. Senior, T. Freire, F. Mackay, M. A. Nobrega, R. Barrès, et al.using a colorimetric 8-hydroxy 2 deoxyguanosine (8-OHdG) ELISA kit (Abcam, Melbourne, Australia, cat # ab201734) according to the manufacturer’s directions. Briefly, 4 µg of DNA was heat denatured (95 °C, 5 min)

(8) MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory MechanismInternational Journal of Molecular SciencesMarch 29, 2023Bogdan Andrei Miu, Ionela Cristina Voinea, Lucian Diamandescu, Anca Dinischiotuusing a commercially available enzyme-linked immunosorbent assay (ELISA) kit purchased from Abcam (ab201734; Cambridge, UK). Previously, DNA from MRC-5 cells exposed to TiO2 NPs was isolated and quantified. Afterward,

(9) Prevalence of Hypertension and Obesity: Profile of Mitochondrial Function and Markers of Inflammation and Oxidative StressAntioxidants (Basel)January 10, 2023Andrés García-Sánchez, Luis Gómez-Hermosillo, Jorge Casillas-Moreno, Fermín Pacheco-Moisés, Tannia Isabel Campos-Bayardo, Daniel Román-Rojas, Alejandra Guillermina Miranda-DíazDNA 8-Hydroxy-2′-Deoxyguanosine (8-OHG) The assay was performed with the reagents kit 8-OHG (No. ab201734 Abcam®, Cambridge, United Kingdom). The competitive ELISA assay was performed with 50 µL of a serum

(10) Polymerized human cord hemoglobin assisted with ascorbic acid as a red blood cell substitute alleviating oxidative stress for blood transfusionFrontiers in bioengineering and biotechnologyJanuary 1, 2023Kong W, Zhou W, He Z, Zhang X, Li S, Zhong R, Liu J.glutathione peroxidase (GPx) assay kit (#ab102530), and an 8-hydroxy 2 deoxyguanosine (8-OHdG) assay kit (#ab201734) were purchased from Abcam (Cambridge, MA, United States). A total antioxidant capacity (T-AOC) assay

(11) Interleukin-33 Induces Neutrophil Extracellular Trap (NET) Formation and Macrophage Necroptosis via Enhancing Oxidative Stress and Secretion of Proatherogenic Factors in Advanced AtherosclerosisAntioxidants (Basel)November 26, 2022Manoj Kumar Tembhre, Mukesh Kumar Sriwastva, Milind Padmakar Hote, Shikha Srivastava, Priyanka Solanki, Shafaque Imran, Ramakrishnan Lakshmy, Alpana Sharma, Kailash Jaiswal, Ashish Datt Upadhyayanalyzed by estimating the concentration of 8-hydroxy 2 deoxyguanosine (8-OHdG) (Abcam, Cambridge, UK, ab201734), malondialdehyde (MDA)/lipid peroxidation (Abcam, Cambridge, UK, ab118970), GSH+GSSG (total glutathione)/GSH

(12) Preliminary Effects of Osteopathic Manipulative Medicine on Reactive Oxygen Species in Parkinson’s Disease: A Randomized Controlled Pilot StudyCureusNovember 14, 2022James Docherty, Joerg R Leheste, Jayme Mancini, Sheldon YaoUSA) and according to the manufacturer’s instructions: MDA (ab238537), 3-NT (ab113848), and 8-OHdG (ab201734) for oxidative damage. Dityrosine was analyzed using the Japan Institute for the Control of Aging (JaICA)

(13) Sirtuin5 protects colorectal cancer from DNA damage by keeping nucleotide availabilityNature CommunicationsOctober 17, 2022Hao-Lian Wang, Yan Chen, Yun-Qian Wang, En-Wei Tao, Juan Tan, Qian-Qian Liu, Chun-Min Li, Xue-Mei Tong, Qin-Yan Gao, Jie Hong, et al.LoVo cells at 48 h post-transfection with control siRNA or SIRT5 siRNAs using an ELISA kit from Abcam (ab201734, USA). Cells treated with 10 μM H2O2 for 30 min served as a positive control. Briefly, DNA was purified

(14) A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative StressAntioxidants (Basel)October 14, 2022Pooja D. Patil, Ana Clara Melo, Brian M. Westwood, E. Ann Tallant, Patricia E. Gallaghercages the day before the rats were sacrificed. Urinary 8-OHdG was measured using an in vitro ELISA kit (ab201734, Abcam, Cambridge, UK). Urinary 8-OHdG data are expressed as ng/mL/24 h. 2.7. Quantification of Catalase

(15) Ameliorating high-fat diet-induced sperm and testicular oxidative damage by micronutrient-based antioxidant intervention in ratsEuropean Journal of NutritionOctober 1, 2022Md Mustahsan Billah, Saroj Khatiwada, Virginie Lecomte, Margaret J. Morris, Christopher A. Maloneydetermined using 8-hydroxy 2 deoxyguanosine ELISA kit as per the manufacturer’s instructions (Abcam Cat ID: ab201734). The 8-OHdG concentration in each sample was calculated by five-parameter logistic regression interpolated

(16) Wild-type IDH1 inhibition enhances chemotherapy response in melanomaJournal of Experimental & Clinical Cancer ResearchSeptember 24, 2022Mehrdad Zarei, Omid Hajihassani, Jonathan J. Hue, Hallie J. Graor, Alexander W. Loftus, Moeez Rathore, Ali Vaziri-Gohar, John M. Asara, Jordan M. Winter, Luke D. Rothermelwavelength at 535 nm on a GloMax plate reader. 8-hydroxy-2-deoxyguanosine (8-OHdG) was measured (Abcam, AB201734) per the manufacturer’s instructions. For apoptosis, the caspase3/7 (Caspase-GloTM Promega G8090) level

(17) Antioxidant Therapy Significantly Attenuates Hepatotoxicity following Low Dose Exposure to Microcystin-LR in a Murine Model of Diet-Induced Non-Alcoholic Fatty Liver DiseaseAntioxidants (Basel)August 22, 2022Apurva Lad et al.(8-OHDG) Free 8-OHDG levels in the urine of animals were quantified using 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734 Abcam®, Cambridge, UK) by following the manufacturer’s protocol. Fifty 50 µL of undiluted urine was

(18) Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signalingImmunityAugust 9, 2022Xian H, Watari K, Sanchez-Lopez E, Offenberger J, Onyuru J, Sampath H, Ying W, Hoffman HM, Shadel GS, Karin M.Lipofectamine™ 3000 Transfection Reagent,Invitrogen,Cat#L3000001 LDH-Glo™ Cytotoxicity Assay,Promega,Cat#J2380 8-hydroxy 2 deoxyguanosine ELISA Kit,Abcam,Cat#ab201734 Experimental Models: Cell Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines HEK293T,ATCC,ATCC®

(19) Partial freezing of rat livers extends preservation time by 5-foldNature communicationsJuly 15, 2022Tessier SN, de Vries RJ, Pendexter CA, Cronin SEJ, Ozer S, Hafiz EOA, Raigani S, Oliveira-Costa JP, Wilks BT, Lopera Higuita M, et al.ab133061), tumor necrosis factor-alpha (TNFα, Abcam, ab236712), 8-hydroxy 2 deoxyguanosine (8-OHdG, Abcam, ab201734), Albumin (Abcam, ab108789), Lactate Dehydrogenase (LDH, MyBioSource, MBS2018912), Flavin Mononucleotide

(20) DNA Damage in Circulating Hematopoietic Progenitor Stem Cells as Promising Biological Sensor of FrailtyThe Journals of Gerontology: Series AJuly 5, 2022Chiara Grasselli et al.8-Hydroxy-2-deoxy guanosine (8-OH-dG) was analyzed in duplicate by enzyme-linked immunosorbent assay (ab201734, 8-hydroxy-2-deoxy guanosine, ELISA kit, Abcam, Cambridge, UK), according to literature (32) and the

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8-hydroxy 2 deoxyguanosine ELISA Kit from Abcam

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8-hydroxy 2 deoxyguanosine ELISA Kit from Abcam

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