MojoSort(TM) Mouse CD8 T Cell Isolation Kit 480035 from BioLegend

Product Specs
ItemMojoSort(TM) Mouse CD8 T Cell Isolation Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number480035
Quantity200 tests
ApplicationsCell Sep - Neg
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD8
Cell Type (standardized)CD8+ T Cell
Conjugate/Tag/LabelMOJO

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysisSTAR ProtocolsDecember 1, 2023Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, et al.spleen tissue of 6–8 week-male C57BL/6 mice with a MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035) according to the standard protocol.c.After separation, firstly incubate the isolated CD8+ T cells with

(2) ARID1A Deficiency Regulates Anti-Tumor Immune Response in Esophageal AdenocarcinomaCancers (Basel)November 12, 2023Le Zhang, Yueyuan Zheng, Wenwen Chien, Benjamin Ziman, Sandrine Billet, H. Phillip Koeffler, De-Chen Lin, Neil A. Bhowmicksolution (4308151, eBioscience, San Diego, CA, USA). The CD8+ MojoSort Mouse CD8+ T cell isolation kit (480035, BioLegend, San Diego, CA, USA) was used to purify naïve CD8+ T cells from freshly harvested spleens.

(3) The histone demethylase Utx controls CD8(+) T-cell-dependent antitumor immunity via epigenetic regulation of the effector functionCancer scienceJuly 1, 2023Noda H, Suzuki J, Matsuoka Y, Matsumoto A, Kuwahara M, Kamei Y, Takada Y, Yamashita M.Utx KO mice, WT OT‐1 Tg mice, or Utx KO OT‐1 Tg mice using a MojoSort CD8+ T Cell Isolation Kit (cat# 480035; BioLegend) with biotin anti‐CD44 mAb (cat#103004; BioLegend). The cells (7.5 × 105 cells) were then

(4) Senescent CD8 + T cells acquire NK cell‐like innate functions to promote antitumor immunityCancer ScienceJuly 1, 2023Toshio Kakuda, Junpei Suzuki, Yuko Matsuoka, Tadahiko Kikugawa, Takashi Saika, Masakatsu Yamashitain vitro CD8+ T cells were prepared from spleen using a MojoSort Mouse CD8+ T cell isolation kit (cat#480035; BioLegend). In naïve CD8+ T (CD44lowCD62Lhigh) cell preparation, biotin anti‐CD44 mAb (cat#103004;

(5) Adhesion analysis via a tumor vasculature-like microfluidic device identifies CD8(+) T cells with enhanced tumor homing to improve cell therapyCell reportsMarch 28, 2023Camargo CP, Muhuri AK, Alapan Y, Sestito LF, Khosla M, Manspeaker MP, Smith AS, Paulos CM, Thomas SN.assays,Critical commercial assays,Critical commercial assays ,, MojoSort Mouse CD8a Selection Kit,Biolegend,Cat#480035 EasySep Human CD8+ T cell Enrichment Kit,STEMCELL,Cat#19053 Dynabeads Mouse T-Activator CD3/CD28,Gibco,Cat#11453D Dynabeads

(6) Single-cell RNA sequencing reveals the suppressive effect of PPP1R15A inhibitor Sephin1 in antitumor immunityiScienceFebruary 17, 2023Rongjing Wang, Yuchao Zhang, Shiwei Guo, Siyu Pei, Wei Guo, Zhenchuan Wu, Hailong Wang, Minghui Wang, Yizhe Li, Yufei Zhu, et al.spleen tissue of adult male C57BL/6 mice with a MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480035) according to the standard protocol. The isolated CD8+ T cells were first incubated with reagents from

(7) GOT1 regulates CD8+ effector and memory T cell generationCell ReportsJanuary 12, 2023Wei Xu, Chirag H. Patel, Liang Zhao, Im-Hong Sun, Min-Hee Oh, Im-Meng Sun, Rachel S. Helms, Jiayu Wen, Jonathan D. Powellplate-bound anti-CD3 activation, CD8+ T cells were isolated (MojoSort™ Mouse CD8 T cell Isolation Kit, Cat#: 480035, Biolegend). 2 million cells at concentration 1 E6 mL−1 were activated in 6-well plate coated with 5

(8) Absence of TSC1 Accelerates CD8⁺ T cell-mediated Acute Cardiac Allograft RejectionAging and diseaseOctober 1, 2022Liang Tan, Yanan Xu, Gongbin Lan, Hongxia Wang, Zhanfeng Liang, Zhaoqi Zhang, Qianchuan Tian, Yangxiao Hou, Yong Zhao, Xubiao Xieprimary wild-type (WT) mice or TSC1-/- mice using the Mojosort Mouse CD8+T Cell Isolation Kit (Cat#: 480035; BioLegend). The purity of enriched CD8+T cells was > 97%. To detect whether TSC1 deficiency enhanced

(9) Mesothelial cell-derived antigen-presenting cancer-associated fibroblasts induce expansion of regulatory T cells in pancreatic cancerCancer CellJune 1, 2022Huocong Huang, Zhaoning Wang, Yuqing Zhang, Rachana N. Pradhan, Debolina Ganguly, Raghav Chandra, Gilbert Murimwa, Steven Wright, Xiaowu Gu, Ravikanth Maddipati, et al.T cell isolation kit,Biolegend,Cat# 480033 MojoSort mouse CD8 T cell isolation kit,Biolegend,Cat# 480035 iScript cDNA Synthesis Kit,Bio-Rad,Cat# 1708891 iTaq Universal SYBR® Green Supermix,Bio-Rad,Cat# 1725121 ProLong Gold Antifade Reagent with DAPI

(10) SMAD4 TGF-β–independent function preconditions naive CD8+ T cells to prevent severe chronic intestinal inflammationJournal of Clinical InvestigationApril 15, 2022Ramdane Igalouzene, Hector Hernandez-Vargas, Nicolas Benech, Alexandre Guyennon, David Bauché, Célia Barrachina, Emeric Dubois, Julien C. Marie, Saïdi M’Homa SoudjaStopfl/fl Rosa26YFP mice were isolated using the Mojosort negative selection kit from BioLegend (catalog 480035). Naive (CD44–) and memory/experienced (CD44+) CD8+ T cells were then sorted on a BD FACSAria II based

(11) miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell levelEMBO reportsApril 5, 2022Wang D, Xiu J, Zhao J, Luo J.T4 DNA ligase,NEB,M0202S 100 bp ladder,NEB,N3231V MojoSort™ Mouse CD8 T Cell Isolation Kit,Biolegend,480035 Xl‐10 Gold competent cells,Homemade,N/A "Nitrocellulose Membrane, 0.2 µm",Bio‐Rad,1620112 TEMED,Sigma,T9281‐25ML Super

(12) Detection of CD8+ T cell-mediated immune responses to bacterial infection in miceSTAR ProtocolsJanuary 3, 2022Jun Wu, Gen Li, Dan Li, Jiawen Zhou, Zhongjun Dong, Peng Jiangcommercial isolation kits are available, e.g., Miltenyi biotec,Cat#130-104-075; and Biolegend, Cat#480035) as described below:i.Put a 70 μm-cell strainer on a 50 mL centrifuge tube.ii.Place the spleen on the

(13) Implantable optical fibers for immunotherapeutics delivery and tumor impedance measurementNature CommunicationsDecember 1, 2021Ai Lin Chin, Shan Jiang, Eungyo Jang, Liqian Niu, Liwu Li, Xiaoting Jia, Rong Tonganalysis Tumor CD8+ TILs were isolated using MojoSort TM mouse CD8 cell isolation kit (Biolegend, catalog # 480035) following manufacturer’s instruction. Isolated CD8+ TILs were numerated and plated at a density of

(14) CXCL2 combined with HVJ-E suppresses tumor growth and lung metastasis in breast cancer and enhances anti-PD-1 antibody therapyMolecular Therapy - OncolyticsMarch 26, 2021Yi Chun Pan, Tomoyuki Nishikawa, Chin Yang Chang, Jiayu A. Tai, Yasufumi Kanedaisolated from all the treated mice splenocytes using the Mojo Sort mouse CD8 T cell Isolation Kit (480035, Biolegend) following the manufacturer’s protocol. The isolated CD8+ T cells (5 × 104 cells in 50 μL

(15) In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouseSTAR protocolsMarch 19, 2021Zeng W, Yin X, Jiang Y, Jin L, Liang W.commercial assays,Critical commercial assays ,,, MojoSort Mouse CD8 T cell Isolation Kit,BioLegend,Cat # 480035,Cat # 480035 Mojo buffer,BioLegend,Cat # 480017,Cat # 480017 10× RBC lysis buffer,eBioscience,Cat

(16) Competition for Active TGFβ Cytokine Allows for Selective Retention of Antigen-Specific Tissue- Resident Memory T Cells in the Epidermal NicheImmunityJanuary 12, 2021Hirai T, Yang Y, Zenke Y, Li H, Chaudhri VK, De La Cruz Diaz JS, Zhou PY, Nguyen BA, Bartholin L, Workman CJ, et al.,Sigma Aldrich,Cat# H7904–5MG Critical Commercial Assays,, MojoSort™ Mouse CD8 T Cell Isolation Kit,BioLegend,Cat# 480035 TaqMan™ Gene Expression Master Mix,Applied Biosystems,Cat# 4369016 RNeasy Mini Kit,QIAGEN,Cat# 74104 High-Capacity

(17) Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8⁺ T Cells and MacrophagesInternational journal of nanomedicineJanuary 1, 2021Luo ZW, Xia K, Liu YW, Liu JH, Rao SS, Hu XK, Chen CY, Xu R, Wang ZX, Xie H.

(18) Nr4a1 and Nr4a3 Reporter Mice Are Differentially Sensitive to T Cell Receptor Signal Strength and DurationCell ReportsNovember 3, 2020Emma Jennings, Thomas A.E. Elliot, Natasha Thawait, Shivani Kanabar, Juan Carlos Yam-Puc, Masahiro Ono, Kai-Michael Toellner, David C. Wraith, Graham Anderson, David Bendingviability dye,eBioscience,Cat# 65-0865-14 MoJo Sort nanobeads: CD8 T Cell Isolation Kit,BioLegend,Cat# 480035 MoJo Sort nanobeads: naive CD4 T Cell Isolation Kit,BioLegend,Cat# 480039 ,, Deposited Data,Deposited

(19) Manganese is critical for antitumor immune responses via cGAS-STING and improves the efficacy of clinical immunotherapyCell researchNovember 1, 2020Lv M, Chen M, Zhang R, Zhang W, Wang C, Zhang Y, Wei X, Guan Y, Liu J, Feng K, et al.

(20) PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell DysfunctionCell reportsOctober 20, 2020Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, Wang L, Jiang Y, Clay R, Wei X, et al.Recombinant Murine M-CSF,Peprotech,Cat #212–12 Critical Commercial Assays,, MojoSort Mouse CD8 T cell Isolation Kit,Biolegend,Cat # 480035 MojoSort Mouse CD4 T cell Isolation Kit,Biolegend,Cat # 480005 10* Permeabilization buffer,eBioscience,Cat

Write a Review

(1)

11/16/2020Great Murine CD8 Isolation Kit Meng SunThe isolation is 90% clean and CD8s are always enriched.
(1)

1/10/2020Good Isolation Kit to Recover Untouched Mouse CD8+ T CellsDr. Min Hee OhWe are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)

1/6/2020Good BioLegend Mouse CD8 T Cells Isolation Kit Wei XuYou can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.
(1)

6/18/2019Easy-to-Use Isolation Kit Meng SunThis kit is a great alternative to other costly brands. The purity is great, the yield is good, and changing to this brand has cut our lab's cost by a few thousands of dollar a year.

MojoSort(TM) Mouse CD8 T Cell Isolation Kit from BioLegend

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MojoSort(TM) Mouse CD8 T Cell Isolation Kit from BioLegend

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