Description
FAM174E Belongs to the FAM174 subfamily. a transmembrane protein (TP) is a protein that goes from one side of a membrane through to the other side of the membrane. Many TPs function as gateways or "loading docks" to deny or permit the transport of specific substances across the biological membrane, to get into the cell, or out of the cell as in the case of waste byproducts. As a response to the shape of certain molecules these "freight handling" TPs may have special ways of folding up or bending that will move a substance through the biological membrane.A transmembrane protein is a polytopic protein that spans an entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FAM47E in samples. An antibody specific for FAM47E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM47E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM47E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM47E bound in the initial step. The color development is stopped and the intensity of the color is measured