MojoSort(TM) Mouse CD4 T Cell Isolation Kit 480033 from BioLegend

Product Specs
ItemMojoSort(TM) Mouse CD4 T Cell Isolation Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number480033
Quantity200 tests
ApplicationsCell Sep - Neg
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD4
Cell Type (standardized)CD4+ T Cell
Conjugate/Tag/LabelMOJO

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansionNature communicationsMay 24, 2023Park JS, Kim JH, Soh WC, Kim NY, Lee KS, Kim CH, Chung IJ, Lee S, Kim HR, Jun CD.Canada, cat# 19851, cat# 19852, cat # 19853) or MojoSort™ Magnetic Cell Separation (BioLegend, cat# 480033) according to the manufacturer’s instructions. Briefly, to obtain single cell suspensions, lymph nodes

(2) Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometrySTAR protocolsDecember 16, 2022Tatsumi N, Davila-Pagan A, Kumamoto Y.marker epitopes intact.c.Isolate CD4T cell with the Mouse CD4T Cell Isolation Kit (BioLegend Cat#: 480033 or StemCell Technologies Cat#: 19852) according to the manufacturer’s Protocol.d.Wash and suspend the

(3) TNFR1 Contributes to Activation-Induced Cell Death of Pathological CD4(+) T Lymphocytes During Ischemic Heart FailureJACC. Basic to translational scienceOctober 1, 2022Kumar V, Rosenzweig R, Asalla S, Nehra S, Prabhu SD, Bansal SS.cells from WT or TNFR1−/− mice were isolated using the MojoSort CD4+ T-cell isolation kit (Catalog #480033, BioLegend) and the RoboSep cell separation system (Stemcell Technologies) following the manufacturers’

(4) SNX9-induced membrane tubulation regulates CD28 cluster stability and signallingeLifeJanuary 20, 2022Ecker M, Schregle R, Kapoor-Kaushik N, Rossatti P, Betzler VM, Kempe D, Biro M, Ariotti N, Redpath GM, Rossy J.(Genotype: B6.Cg-Tg(TcraTcrb)425Cbn/J) using the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend, Cat# 480033). Isolated T cells were seeded at 1 × 106 cells/mL in complete RPMI supplemented with 40 U/mL murine

(5) Effective CD4 T cell priming requires repertoire scanning by CD301b ⁺ migratory cDC2 cells upon lymph node entryScience ImmunologyDecember 24, 2021Naoya Tatsumi, Alicia L. Codrington, Jihad El-Fenej, Varoon Phondge, Yosuke Kumamotoindicated donor mice with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher) or CellTrace

(6) The kinase PDK1 regulates regulatory T cell survival via controlling redox homeostasisTheranosticsSeptember 13, 2021Peiran Feng et al.peripheral lymph nodes. Total CD4+ T cells were bead-isolated used Mouse CD4 T Cell Isolation Kit (Cat#480033, Biolegend). Naïve T cells (CD4+YFP-CD44-CD62L+), Treg cells (CD4+YFP+) cells were sorted on BD FACS

(7) BFAR coordinates TGFβ signaling to modulate Th9-mediated cancer immunotherapyThe Journal of experimental medicineJuly 5, 2021Pei S, Huang M, Huang J, Zhu X, Wang H, Romano S, Deng X, Wang Y, Luo Y, Hao S, et al.from enriched CD4+ T cells via negative selection with the MojoSort Mouse CD4+ T Cell Isolation Kit (480033; BioLegend) according to the manufacturer’s instructions. The sorted naive CD4+ T cells were stimulated

(8) Enhanced Susceptibility of Galectin-1 Deficient Mice to Experimental ColitisFrontiers in ImmunologyJune 28, 2021Raquel Fernandez-Perez, Mercedes Lopez-Santalla, Rebeca Sánchez-Domínguez, Omaira Alberquilla, Irene Gutiérrez-Cañas, Yasmina Juarranz, Juan A. Bueren, Marina I. Garinspleens using Mojosort Mouse CD4 T Cell isolation kit according to the manufacturer’s instructions (ref. 480033; BioLegend, San Diego, CA). Regulatory T cells (Treg cells) were enriched from total CD4+ T cells by

(9) Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung CancerCancer immunology researchNovember 1, 2020Qin J, Zhang X, Tan B, Zhang S, Yin C, Xue Q, Zhang Z, Ren H, Chen J, Liu M, et al.

(10) PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell DysfunctionCell reportsOctober 20, 2020Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, Wang L, Jiang Y, Clay R, Wei X, et al.

(11) Self-Assembled Multivalent Aptamer Nanoparticles with Potential CAR-like Characteristics Could Activate T Cells and Inhibit Melanoma GrowthMolecular therapy oncolyticsJune 26, 2020Bai C, Gao S, Hu S, Liu X, Li H, Dong J, Huang A, Zhu L, Zhou P, Li S, et al.

(12) Lattice Light-Sheet Microscopy Multi-dimensional Analyses (LaMDA) of T-Cell Receptor Dynamics Predict T-Cell Signaling StatesCell systemsMay 20, 2020Rosenberg J, Cao G, Borja-Prieto F, Huang J.were then harvested by negative isolation (MojoSort Mouse CD4 T Cell Isolation Kit, BioLegend, cat: 480033). Cells were washed three times with complete medium and incubated with Alexa488-labeled anti-TCRβ (2

(13) Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophagesPLoS pathogensMay 1, 2020Cheng Y, Kiene NJ, Tatarian A, Eix EF, Schorey JS.

(14) CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE ModelFrontiers in immunologyJanuary 1, 2020Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, Li J, Liang S, Xiong J, Li Z, et al.

(15) T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunityNature immunologyJanuary 1, 2020Jain A, Irizarry-Caro RA, McDaniel MM, Chawla AS, Carroll KR, Overcast GR, Philip NH, Oberst A, Chervonsky AV, Katz JD, et al.

(16) Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitroScientific reportsNovember 22, 2019Nomura S, Takahashi H, Suzuki J, Kuwahara M, Yamashita M, Sawasaki T.CD4+ T cells (CD44low, CD62Lhigh) were prepared using a MojoSort Mouse CD4 T cell Isolation Kit (Cat#480033), BioLegend). Biotinylated anti-CD44 (Cat#103004, IM7) and anti-CD25 antibodies (Cat#102004, PC61) were

(17) Cross-Species Single-Cell Analysis of Pancreatic Ductal Adenocarcinoma Reveals Antigen-Presenting Cancer-Associated FibroblastsCancer discoveryAugust 1, 2019Elyada E, Bolisetty M, Laise P, Flynn WF, Courtois ET, Burkhart RA, Teinor JA, Belleau P, Biffi G, Lucito MS, et al.

(18) Uterine B Cells Exhibit Regulatory Properties During the Peri-Implantation Stage of Murine PregnancyFrontiers in immunologyJanuary 1, 2019Guzman-Genuino RM, Eldi P, Garcia-Valtanen P, Hayball JD, Diener KR.Cell Isolation Kit (480052; Biolegend, San Diego, CA, USA) or MojoSortTM Mouse CD4 T Cell Isolation Kit (480033; BioLegend) according to the manufacturer's instructions. Isolated B cells or CD4+ T cells were routinely

(19) Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4⁺ Th CellsJournal of immunology (Baltimore, Md. : 1950)October 1, 2017Read KA, Powell MD, Baker CE, Sreekumar BK, Ringel-Scaia VM, Bachus H, Martin RE, Cooley ID, Allen IC, Ballesteros-Tato A, et al.the spleens of L. monocytogenes–infected mice using the BioLegend MojoSort Isolation Kit (cat. no. 480033), per the manufacturer’s instructions. For subsequent flow cytometry analysis, isolated cells were stained

(20) FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic CellsFrontiers in immunologyJanuary 1, 2017Pan YG, Yu YL, Lin CC, Lanier LL, Chu CL.

Write a Review

(1)

11/16/2020Relatively Simple Kit but not Always Enriched CD4s Meng SunFor some experiments, we require a pure CD4 population from the beginning therefore we use quick kits like this for enriching CD4s.
(1)

1/31/2020CD4 Enrichment of Cultured Splenocytes Using MojoSort Troy TrevinoThe MojoSort Mouse CD4 T cell isolation kit works very well to enrich for CD4 cells in a primary splenocyte culture. When used in tandem with a dead cell separation medium like Lympholyte-M, high ... Read Review
(1)

12/27/2019Pan CD4 T Cell Isolation Kit from Mouse Wei XuYou can use this kit to get acceptable yield and purity to isolate CD4 cells from mouse with reasonable price.
(1)

4/17/2018Isolating CD4 T Cells Matthew SmithI used this kit side by side with StemCell Technologies Mouse CD4 negative selection kit. Biolegend's product returned similar cell numbers and purity and is MUCH cheaper. The protocol is slightly ... Read Review
(1)

9/7/2017Isolation Of Mouse CD4 T Cell (Negative Selection) Jeeho LeeThe purpose of using this kit is to purify the "Untouched" CD4 T cells from murine spleen and lymph nodes. Isolated T cells were cultured in vitro or were transferred into mice for in vivo study.

Four Key Considerations When Performing Magnetic Cell Separation
February 02, 2016
The separation of specific cells within a complex cell mixture is a challenge that researchers face on a daily basis. Three major techniques have emerged as most widely used: centrifugation-based, fluorescence-activated ... read more

STAR protocols

MojoSort(TM) Mouse CD4 T Cell Isolation Kit from BioLegend

Description

BioLegend

Citations (23)

Reviews

Bench Tips

Four Key Considerations When Performing Magnetic Cell Separation

MojoSort(TM) Mouse CD4 T Cell Isolation Kit from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map