Description
The Replication-Competent Lentivirus (RCL) qPCR Detection Kit is designed to provide a fast (less than 4 hours), sensitive (detects as low as 10 copies/µl), reliable and specific method to detect RCL contamination in both cell culture supernatants and transduced cells by quantitative Polymerase Chain Reaction (qPCR). The kit targets sequences derived from HIV-1 VSV-G genes that are typically retained in helper packaging systems and are absent in lentiviral vectors but would be present if RCL were generated. The kit includes enough reagents for 100 reactions, including standard curve and controls