Fig 1: BYF inhibits CSE-induced cellular senescence. The protein levels of senescence markers p16, p21 and p27 were detected by Western blot (A and B). The secretion of IL-6 (C), IL-8 (D), and CCL3 (E) into the medium was measured by ELISA. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; #Compared into CSE group, P<0.05; ##Compared into CSE group, P<0.01.
Fig 2: BYF activates klotho transcription by upregulating the expression of transcription factor ZNF263. ZNF263 binding klotho promoter sequence with JASPAR and USCS databases (A). The protein level of ZNF263 in BEAS-2B cells with CSE exposure and BYF treatment was detected by Western blot (B and C). The protein levels of ZNF263, klotho and senescence markers in BEAS-2B cells with CSE exposure and ZNF263-expression plasmid transfection were detected by Western blot (D–F). The secretion of IL-6 (G), IL-8 (H), and CCL3 (I) into the medium was measured by ELISA. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; #Compared into CSE group, P<0.05; ##Compared into CSE group, P<0.01; ###Compared into CSE group, P<0.001.
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