Fig 1: Characterization of hOBs. (a) Representative phase contrast and immunofluorescence images of isolated hOBs. (b) Mineral matrix deposition was evaluated by ARS. Results are expressed as the median and 1st and 3rd quartile of at least three independent experiments (n = 5) (* p < 0.05 vs. CTRL). (c) Expression of typical osteoblastic markers (ALP, Runx2, OC, OPN, and COL1a1) analyzed by flow cytometry (representative histograms).
Fig 2: Effect of MK-4 treatment on 2-D cultured hOBs. hOBs were cultured in osteogenic medium (OM) combined or not with MK-4 at 10 µM (OM + MK-4) for 14 days. Mineral matrix deposition was evaluated by ARS and results are expressed as (a) the median and 1st and 3rd quartile of at least three independent experiments (n = 10). (b,c) Expression of typical osteoblast markers (ALP, Runx2, OC, OPN, and COL1a1) analyzed both at the mRNA and protein level, by RT-qPCR and flow cytometry, respectively. Data are presented as mean ± standard deviation (SD) of at least three independent experiments (n = 5). (* p < 0.05 vs. OM).
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