Fig 1: ROC plots for females, using circulating NLRP3 levels as test variable and MetS and its individual components as state variable. AUC is “area under the curve”, 95% CI is 95% confidence interval of AUC, p < 0.05 is considered significant. The five components of MetS- central obesity, hypertension, hyperglycemia, low HDL-C, and hypertriglyceridemia; and the full MetS has been plotted in subfigures (a–f) respectively.
Fig 2: ROC curve analysis of CSF NLRP3 and other parameters used for CABM prognosis. (A) AUC value of CSF NLRP3 (AUC = 0.8875, 95% CI: 0.7644–1.011, p < 0.001) for prediction of CABM prognosis. The cutoff values were 2840 ng/ml with a sensitivity of 76.47% and a specificity of 80%. (B) AUC value of NLR (AUC = 0.7208, 95% CI: 0.5512–0.8905, p = 0.04521) for prediction of CABM prognosis. (C) AUC value of the lymphocyte count (AUC = 0.8063, 95% CI: 0.6587–0.9538, p < 0.01) for prediction of CABM prognosis. (D) AUC value of the monocyte count (AUC = 0.7458, 95% CI: 0.5805–0.9112, p = 0.02579) for prediction of CABM prognosis.
Fig 3: Box-plots showing increasing circulating levels of IL-1ß (a), CRP (b), TNF-a (c) and NLRP3 (d) with increasing MetS components in females. The yellow and gray boxes show third and first quartile, respectively, and the meeting point signifies the median.
Fig 4: Relationship between CSF NLRP3 and other parameters. (A) CSF NLRP3 was positively correlated with the NLR (r = 0.4494, R 2 = 0.202, p = 0.0077). (B) CSF NLRP3 was positively correlated with the PLR (r = 0.8596, R 2 = 0.7347, p < 0.0001). (C) CSF NLRP3 was positively correlated with Qalb (r = 0.6296, R 2 = 0.3965, p < 0.0001). (D) CSF NLRP3 was positively correlated with QIgG (r = 0.6504, R 2 = 0.423, p < 0.0001). (E) CSF NLRP3 was negatively correlated with the lymphocyte count (r = -0.5649, R 2 = 0.3192, p = 0.0005). (F) CSF NLRP3 and the monocyte count were not correlated (r = -0.2203, R 2 = 0.04853, p = 0.2106).
Fig 5: The receiver operating characteristic (ROC) curve analysis of CSF NLRP3 for the diagnosis of CABM. (A) NLRP3 levels showed a good area under the curve (AUC) value in the CABM vs. non-CABM comparison (AUC = 0.8784, 95% CI: 0.7932–0.9636, p < 0.0001). The cutoff values of CSF NLRP3 were 860.9 ng/ml with a sensitivity of 79.4% and a specificity of 88.9%. (B) NLRP3 showed a good area under the curve (AUC) value in the CABM vs. VM comparison (AUC = 0.8279, 95% CI: 0.7182–0.9377, p < 0.0001). The cutoff values of CSF NLRP3 were 892.6 ng/ml with a sensitivity of 76.47% and a specificity of 80%.
Supplier Page from CUSABIO Technology LLC for Human NACHT, LRR and PYD domains-containing protein 3 (NLRP3/C1orf7/CIAS1/NALP3/PYPAF1) ELISA Kit