TGF beta-1 Human/Mouse Uncoated ELISA Kit 88-8350-88 from Thermo Fisher Scientific

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Nature communications
May 11 2020

Fig 1: MEVsCa act anti-inflammatory on endothelial cells.a TGF-ß1 vesicles align along the endothelial cells of blood vessels in liver tissue of C. albicans-infected mice as shown by immunohistochemistry as compared with uninfected mice. Liver tissue was collected 24 h after C. albicans infection of mice. Staining– red: TGF-ß1 and orange: nucleic acids. Bars: 10 µm. Representative stainings of four experiments (n = 4 mice) are shown. b Tracking of purified blood cell EVs by DLSM. EVs were isolated from 5 × 107 uninfected or opsonized C. albicans-infected blood cells (BCEVs or BCEVsCa, respectively) by polymer precipitation. Representative profiles of three donors are shown. c BCEVsCa significantly increase compared with BCEVs (data are presented as mean values + /- SD, p = 0.0031, unpaired two-tailed t test, n = 3 donors). d Profiles of BCEVs or BCEVsCa as determined by DLSM using NanoSight NTA 3.2 software. Graphs were generated by overlaying the size distribution of BCEVs and BCEVsCa from three donors. e TGF-ß1 is present in the membrane fraction of BCEVsCa, but not in BCEVs and the cytosol fractions of BCEVs or BCEVsCa. BCEVs or BCEVCaS were isolated from 5 × 107 blood cells, cytosol and membrane fractions separated, and TGF-ß1 detected by western blot (see uncropped WB in Supplementary Fig. 7e). f TGF-ß1 on BCEVsCa binds to TGF-ßRII on HUVECs. HUVECs were incubated with BCEVs or BCEVsCa for 30 min, stained for TGF-ß1 and TGF-ßRII, and TGF-ß1/TGF-ßRII complexes were detected by PLA. PLA staining– red: TGF-ß1/TGF-ßRII complexes, blue: DNA. Bars: 10 µm. Representative data of three experiments (n = 3 donors) are shown in e, f. g HUVECs incubated with BCEVsCa show increased PLA fluorescence intensity compared with HUVECs incubated with BCEVs as measured by ZEN Black 2011 software (data are presented as mean values + /- SD, p < 0.0001, unpaired two-tailed t test, n = 32 individual cell from n = 3 donors). h HUVECs treated with BCEVsCa but not with BCEVs upregulated TGFB1 expression (data are presented as mean values + /- SD, p = 0.0106, unpaired two-tailed t test, n = 5 donors). HUVECs (3 × 106) were treated for 6 h with BCEVs or BCEVsCa each isolated from 5 × 108 blood cells. Purified RNA was evaluated by comparative qPCR. i HUVECs treated with BCEVsCa for 6 h increase intracellular TGF-ß1 as observed in CLSM. Staining– green: TGF-ß1 and orange: nucleic acids. Bars: 10 µm. Representative data of three independent experiments (n = 3 donors) are shown. j Intracellular TGF-ß1 as measured by ZEN 2011 (data are presented as mean values + /- SD, p < 0.0001, unpaired two-tailed t test, n = 39 individual cells from n = 3 donors). k BCEVCa-treated HUVECs increase intracellular TGF-ß1 as measured by ELISA (data are presented as mean values + /- SD, p = 0.0018, unpaired two-tailed t test, n = 4 donors). l HUVECs treated with recombinant TGF-ß1 increase TGFB1 expression in a dose-dependent manner compared to treatment with BCEVs (data are presented as mean values + /- SD, p = 0.0470, p = 0.0213, unpaired two-tailed t test, n = 3 donors). m HUVECs incubated with C. albicans show no increase in TGFB1 expression (data are presented as mean values + /- SD, p = 0.9839, unpaired two-tailed t test, n = 3 donors).
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Frontiers in Immunology
January 05 2022

Fig 2: Fbxw7 deletion upregulates TGF-ß expression in lung fibrosis model induced by bleomycin. qRT-PCR analysis of Tnf-a, Il1ß, Il6, and Il10 relative mRNA expression of AMs (A) after Fbxw7fl/fl and LysM+Fbxw7fl/fl mice were administered PBS or bleomycin for 21 days to induce pulmonary fibrosis. After a 21d period of bleomycin-induced lung injure, qRT-PCR analysis of Tgfb1 relative mRNA expression in lung tissue and AMs (B), TGF-ß content in BALF was detected by ELISA (C). Tgfb1 relative mRNA expression in BAL cells was analyzed by qRT-PCR (D), TGF-ß content in serum and BALF was detected by ELISA (E) after 10 days of LPS-induced lung injury. Macrophages from Fbxw7fl/fl and LysM+Fbxw7fl/fl mice were stimulated with IL-4 (30 ng/ml) and treated with or without TGF-ß neutralizing antibody for 12 h, then co-cultured with MEF for another 48 h. (F) The schematics of the approach. (G) TGF-ß in the media of the macrophage co-culture system. Data are expressed as mean ± SD of biological duplicates (n=3) and are the representative of three independent experiments. P values were obtained using two-tailed Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant. (H) Representative images of western blot analysis of collagen-I and collagen-III in co-cultured MEF after IL-4 treatment. Densitometric fold change of target proteins based on IL-4 untreated group was calculated. (I) Representative images of western blot analysis of collagen-I and collagen-III in co-cultured MEF after IL-4 with TGF-ß neutralizing antibody (Ab) treatment (3 µg/ml). Iso: Isotype. Relative protein levels were presented by densitometric fold change quantified by ImageJ based on Fbxw7fl/f untreated group, the floating bars indicate the min, mean and max value of biological duplicates (n=3), which are the representative of three independent experiments. P values were obtained using One-way ANOVA with Tukey’s Multiple Comparison test (H) or two-tailed Student’s t test (I). *P < 0.05, **P < 0.01, ***P < 0.001.
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Frontiers in Immunology
January 05 2022

Fig 3: Fbxw7 regulates TGF-ß expression via mediating the ubiquitination of c-Jun. (A) Immunoblot analysis of phosphorylated and/or total proteins in lysates of lung tissues obtained from bleomycin-treated Fbxw7fl/fl and LysM+Fbxw7fl/fl mice. Densitometric fold change of target proteins based on Fbxw7fl/fl group was calculated. (B) Immunoblot analysis of c-Jun protein levels in lysates of IL-4 (30 ng/ml) stimulated BMDMs from Fbxw7fl/fl and LysM+Fbxw7fl/fl mice. Densitometric fold change for the relative c-Jun level based on Fbxw7fl/fl untreated group was calculated. (C) Fbxw7fl/fl and LysM+ Fbxw7fl/fl BMDMs stimulated with IL-4 and treated or not with SP600125 (15 µM), qRT-PCR analysis for Tgfb1 relative mRNA expression and ELISA for TGF-ß protein expression. Data are expressed as mean ± SD of biological duplicates (n=5) and are the representative of three independent experiments. P values were obtained using two-tailed Student’s t test, ***P < 0.001. (D) Immunoblot analysis of c-Jun expression in BMDMs treated or not with MG132 (20 µM) for 8 hours and stimulated with IL-4 for the indicated durations. Densitometric fold change of c-Jun based on IL-4, MG132 untreated group was calculated. (E) Immunoblot analysis and relative protein levels of c-Jun in lysates of Fbxw7fl/fl and LysM+Fbxw7fl/fl BMDMs treated with CHX (40 µg/ml) for indicated hours after stimulation with IL-4 for 6 h. (F) Coimmunoprecipitation and immunoblot of BMDMs stimulated with IL-4 for indicated hours. (G) Immunoblot analysis of the K48 ubiquitination of c-Jun in Fbxw7fl/fl and LysM+Fbxw7fl/fl BMDMs stimulated with IL-4 for 6 h. (H) Peritoneal macrophages from LysM+Fbxw7fl/fl (WT) and Fbxw7fl/fl (KO) mice were stimulated with IL-4 for 6 h, coimmunoprecipitation was performed with K48-Ub to pull-down ubiquitylated proteins, followed by IB with c-Jun. Relative protein levels were presented by densitometric fold change quantified by ImageJ, the floating bars indicate the min, mean and max value of biological duplicates and are the representative of three independent experiments. P values were obtained using two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.

Description

The Human/Mouse Transforming Growth Factor beta 1 (TGFβ1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human/mouse TGFβ1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

Principle of the method

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen

Product Specs
ItemTGF beta-1 Human/Mouse Uncoated ELISA Kit
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number88-8350-88
Quantity10 x 96 tests
ApplicationsELISA
Detection Range8-1,000 pg/mL
ReactivityHuman, Mouse
Sample TypePlasma, 20 µL; Serum, 20 µL; Supernatant, 100 µL
Detection TargetTGF beta-1
Assay TimeHands-On Time: 1 hr 45 min; Time to Results: 24 hr 30 min
NCBI Full Gene Nametransforming growth factor beta 1, transforming growth factor, beta 1
NCBI Gene AliasesTGF-beta1, TGFB, DPD1, TGFbeta, CED, TGFbeta1, IBDIMDE, LAP, Tgfb, Tgfb-1
Research AreaAngiogenesis; Growth Factor

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Website: www.thermofisher.com

(1) Proline is increased in allergic asthma and promotes airway remodelingJCI insightAugust 22, 2023Xu T, Wu Z, Yuan Q, Zhang X, Liu Y, Wu C, Song M, Wu J, Jiang J, Wang Z, et al.homogenates, such as IL-4, IL-5, IFN-γ (BioLegend, 431105, 431204, 430804), TGF-β1 (Thermo Fisher Scientific, 88-8350-88), collagen I (Elabscience, E-EL-M0325c) and IL-13 (PeproTech, 900-K207), were measured using commercial

(2) ZIC2 induces pro-tumor macrophage polarization in nasopharyngeal carcinoma by activating the JUNB/MCSF axisCell Death & DiseaseJuly 21, 2023Qian Liu, Ting Yang, Yu Zhang, Ze-Dong Hu, Yan-Min Liu, Yi-Ling Luo, Shang-Xin Liu, Hua Zhang, Qian ZhongR&D, USA), human MCSF (CHE0042-96, 4Abio, China), and mouse MCSF (DY416-05, R&D, USA), mouse TGF-β (88-8350-88, Invitrogen, USA)and mouse IL-10 (431414, BioLegend, USA) kits. Briefly, a 96-well plate was coated

(3) Leishmania donovani Attenuates Dendritic Cell Trafficking to Lymph Nodes by Inhibiting C-Type Lectin Receptor 2 Expression via Transforming Growth Factor-βMicrobiology spectrumJune 15, 2023Yadav M, Akhtar MN, Mishra M, Kumar S, Kumar R, Shubham, Nandal A, Sen P.above. The culture supernatants were assayed for IL-10 (88-7105-88; Thermo Fisher Scientific) and TGF-β (88-8350-88; Thermo Fisher Scientific) using enzyme-linked immunosorbent assay (ELISA) kits following the manufacturer’s

(4) Transforming growth factor beta 1 (TGF-β1) in COVID-19 patients: relation to platelets and association with the disease outcomeMolecular and Cellular BiochemistryMarch 4, 2023Snezana Zivancevic-Simonovic, Rajna Minic, Vojislav Cupurdija, Marijana Stanojevic-Pirkovic, Olivera Milosevic-Djordjevic, Vladimir Jakovljevic, Olgica Mihaljevicblood cultures using the Human/Mouse TGF beta 1 uncoated ELISA (Thermo Fisher Scientific) ELISA kit, Catalog No. 88-8350-88 according to the manufacturer’s instructions. To activate latent TGF-β1 to the immunoreactive form,

(5) Omega 3 supplementation reduces C-reactive protein, prostaglandin E2 and the granulocyte/lymphocyte ratio in heavy smokers: An open-label randomized crossover trialFrontiers in NutritionDecember 1, 2022Ingrid Elisia, Michelle Yeung, Sara Kowalski, Jennifer Wong, Hossein Rafiei, Roger A. Dyer, Sukhinder Atkar-Khattra, Stephen Lam, Gerald KrystalCayman Chemical Company, Ann Arbor, MI) kits, according to the manufacturers' instructions. TGF-β (cat # 88-8350-88, Thermofisher Scientific, Ottawa, ON, Canada) levels were determined using a slight modification of

(6) The Autism Spectrum Disorder-Associated Bacterial Metabolite p-Cresol Derails the Neuroimmune Response of Microglial Cells Partially via Reduction of ADAM17 and ADAM10International journal of molecular sciencesSeptember 20, 2022Zheng Y, Prince NZ, Peralta Marzal LN, Ahmed S, Garssen J, Perez Pardo P, Kraneveld AD.quantified using mouse TNF-α ELISA kit (430901, BioLegend, San Diego, CA, USA), human/mouse TGF-β ELISA kit (88-8350-88, Invitrogen, Waltham, MA, USA), mouse IL-6 ELISA kit (431301, BioLegend, USA), mouse IL-6R ELISA Kit

(7) Fatty acid nitroalkene reversal of established lung fibrosisRedox biologyApril 1, 2022Koudelka A, Cechova V, Rojas M, Mitash N, Bondonese A, St Croix C, Ross MA, Freeman BA.Invitrogen/ThermoFisher kits: IFN-γ (88-7314-88), IL1-β (88-7013-88), IL-13 (88-7137-88), MCP-1 (88-7391-88), TGF-β (88-8350-88) and TNF-α (88-7324- 88). Statistical analysis. Statistical analyses were performed using GraphPad Prism

(8) Liposomal Glutathione Helps to Mitigate Mycobacterium tuberculosis Infection in the LungsAntioxidants (Basel)March 30, 2022Nala Kachour et al.IFN-γ (catalog #88-7314-88), IL-2 (catalog #88-7024-88), IL-10 (catalog #88-7105-88), TGF-β (catalog #88-8350-88), TNF-α, IL-12 (catalog #BMS6004), and IL-17 (catalog #88-7002-88), as per the manufacturer’s instructions

(9) A Human Conditionally Immortalized Proximal Tubule Epithelial Cell Line as a Novel Model for Studying Senescence and Response to SenolyticsFrontiers in PharmacologyMarch 29, 2022Yi Yang, Milos Mihajlovic, Floris Valentijn, Tri Q. Nguyen, Roel Goldschmeding, Rosalinde MasereeuwCTGF (DY9190-05, R&D System, United Kingdom), TNF-α (88-7346-88, Invitrogen, Carlsbad, CA), and TGF-β1(88-8350-88, Invitrogen, Carlsbad, CA) were used according to the manufacturer’s instructions. 2.6 Statistics All

(10) Myeloid Fbxw7 Prevents Pulmonary Fibrosis by Suppressing TGF-β ProductionFrontiers in ImmunologyJanuary 5, 2022Jia He, Yue Du, Gaopeng Li, Peng Xiao, Xingzheng Sun, Wenjun Song, Lihua Lai, Meng Xia, Jianhua Zhang, Qingqing Wangbronchoalveolar lavage fluid (BALF) or culture supernatants were measured with ELISA kits (Invitrogen, 88-8350-88) according to the manufacturer’s instructions. Statistical Analysis Statistical analysis was performed

(11) Fabrication of 3D Printed Poly(lactic acid)/Polycaprolactone Scaffolds Using TGF-β1 for Promoting Bone RegenerationPolymers (Basel)October 28, 2021Cheng-Hsin Cheng, Ming-You Shie, Yi-Hui Lai, Ning-Ping Foo, Mon-Juan Lee, Chun-Hsu YaoQuantification of Immobilized TGF-β1 We used an enzyme-linked immunosorbent assay (ELISA assay) (Cat.88-8350-88, Invitrogen) for measurement of TGF-β1 concentration on the scaffolds. For the preparation of different

(12) NK Cell-Dependent Antibody-Mediated Immunotherapy Is Improved In Vitro and In Vivo When Combined with Agonists for Toll-like Receptor 2 in Head and Neck Cancer ModelsInternational Journal of Molecular SciencesOctober 14, 2021Mandy Gruijs et al.protocol. The cytokines IFN-γ, TGF-β and TNF-α (eBioscience, San Diego, CA, USA, cat. no. 88-7316-22, 88-8350-88, 39-8329, respectively) and granzyme B (MABTECH, Stockholm, Sweden, cat. no. 3485-3-250/6-250) were

(13) Disulfiram Sensitizes a Therapeutic-Resistant Glioblastoma to the TGF-β Receptor InhibitorInternational Journal of Molecular SciencesSeptember 28, 2021Chan-Chuan Liu, Cheng-Lin Wu, Meng-Xuan Lin, Chun-I Sze, Po-Wu Geansecretions of TGF-β1 were measured using a TGF beta-1 Human/Mouse Uncoated ELISA Kit (Invitrogen, Catalog # 88-8350-88). 4.4. MTT Assay 4000 cells were incubated in 96-well plate for 24 h. The cultured media were replaced

(14) Inhibiting IRE1α-endonuclease activity decreases tumor burden in a mouse model for hepatocellular carcinomaeLifeOctober 26, 2020Pavlović N, Calitz C, Thanapirom K, Mazza G, Rombouts K, Gerwins P, Heindryckx F.

(15) HIF-2α is indispensable for regulatory T cell functionNature communicationsOctober 6, 2020Hsu TS, Lin YL, Wang YA, Mo ST, Chi PY, Lai AC, Pan HY, Chang YJ, Lai MZ.for mouse IFN-γ (88-7314-88), mouse IL-17A (88-7371-88), mouse IL-10 (88-7105-88), and mouse TGF-β (88-8350-88) were purchased from Thermo Fisher. Ovalbumin (A2512), 12-O-Tetradecanoylphorbol 13-acetate (TPA, also

(16) Dissecting the immunosuppressive tumor microenvironments in Glioblastoma-on-a-Chip for optimized PD-1 immunotherapyeLifeSeptember 10, 2020Cui X, Ma C, Vasudevaraja V, Serrano J, Tong J, Peng Y, Delorenzo M, Shen G, Frenster J, Morales RT, et al.

(17) Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to MyofibroblastsScientific reportsJune 24, 2020El-Hattab MY, Nagumo Y, Gourronc FA, Klingelhutz AJ, Ankrum JA, Sander EA.

(18) Immune modulation by complement receptor 3-dependent human monocyte TGF-β1-transporting vesiclesNature communicationsMay 11, 2020Halder LD, Jo EAH, Hasan MZ, Ferreira-Gomes M, Krüger T, Westermann M, Palme DI, Rambach G, Beyersdorf N, Speth C, et al.ELISA (R&D Systems, cat no. DY1679) or Human/Mouse TGF-β1 ELISA Ready-SET-Go! Kit (eBioscience, cat no. 88-8350-88). IL-10, IL-6, and Il-1β were measured using the human IL-10 ELISA Ready-SET-Go! Kit (eBioscience, cat

(19) Trained immunity modulates inflammation-induced fibrosisNature communicationsDecember 11, 2019Jeljeli M, Riccio LGC, Doridot L, Chêne C, Nicco C, Chouzenoux S, Deletang Q, Allanore Y, Kavian N, Batteux F.Human Ref # 88-7346-88), CCL2 (Mouse Ref # 88-7391-88, Human Ref # 88-7399-88), and TGF-β(Mouse Ref # 88-8350-88) was performed by specific mouse ELISA kits from eBiosciences (Invitrogen) (Thermo Fisher Scientific,

(20) Bi- and tri-valent T cell engagers deplete tumour-associated macrophages in cancer patient samplesJournal for immunotherapy of cancerNovember 21, 2019Scott EM, Jacobus EJ, Lyons B, Frost S, Freedman JD, Dyer A, Khalique H, Taverner WK, Carr A, Champion BR, et al.(Biolegend, UK, #430604), IFN-γ (Biolegend, UK, #430104), TGF beta-1 Human/Mouse ELISA Kit (Invitrogen, UK, #88–8350-88) and CD206 (RayBiotech, USA, #ELH-MMR-1), following manufacturers’ instructions. Prior to analysis,

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5/6/2020Easy-to-Use TGF-beta1 ELISA Kit and Less Deviation of Results from both Mouse and Human Cell Line Preparations Shubhankar AmbikeThe kit is quite straight forward to use, the protocol provided is not at all complicated.

Nature communications

Frontiers in Immunology

Frontiers in Immunology

TGF beta-1 Human/Mouse Uncoated ELISA Kit from Thermo Fisher Scientific

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TGF beta-1 Human/Mouse Uncoated ELISA Kit from Thermo Fisher Scientific

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