Intended Uses: This sandwich kit is for the accurate quantitative detection of human Small Nuclear Ribonucleoprotein (also known as SNRNP/SM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SNRNP/SM antibody. SNRNP/SM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SNRNP/SM Antibody is added and binds to SNRNP/SM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SNRNP/SM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SNRNP/SM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm