Description
The Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set is for the quantitative determination of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA coated on a 96-well plate. Standards and samples are added to the wells, and any Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm