Human Guanine nucleotide-binding protein G (GNG13) ELISA Kit from


This assay employs a two-site sandwich ELISA to quantitate GNG13 in samples. An antibody specific for GNG13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GNG13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GNG13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GNG13 bound in the initial step. The color development is stopped and the intensity of the color is measured