GBP6 ELISA Kit (Human) (OKEH04629) from Aviva Systems Biology

Description

Aviva Systems Biology GBP6 ELISA Kit (Human) (OKEH04629) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human GBP6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GBP6 and incubated. The found in the sample or standards competes with the biotinylated GBP6 for limited binding sites on the immobilized anti-Human GBP6 antibody. Excess unbound biotinylated GBP6 and sample or standard GBP6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GBP6 captured in the well and inversely proportional to the amount of GBP6 which was contained in the sample or standard