Biometra TAdvanced Thermal Cycler Series from Analytik Jena

(1) Wilder than intense: higher frequency, variability, and viral flows of porcine circovirus 3 in wild boars and rural farms compared to intensive ones in northern ItalyFrontiers in MicrobiologyJuly 31, 2023Giovanni Franzo, Giulia Faustini, Matteo Legnardi, Giacomo Berto, Mariangela Dal Maso, Viviana Genna, Maria Luisa Menandro, Francesca Poletto, Mattia Cecchinato, Michele Drigo, et al.the primer pairs reported in Franzo et al. (2018b) were used for complete genome characterization. Biometra TAdvanced® Thermal Cycler (Analytik Jena GmbH, Jena, Germany) and Invitrogen™ Platinum™ II Taq Hot-Start DNA polymerase

(2) Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow StripJournal of Analytical Methods in ChemistryJuly 22, 2023Wei Lai, Yongjie Xu, Lin Liu, Huijun Cao, Bin Yang, Jie Luo, Ying Fei(Karlsruhe, Germany), automatic nucleic acid extraction instrument from Tianlong Technology (Xian, China), Biometra TAdvanced 96SG PCR from Analytik Jena (Jena, Germany), and HM3035 XYZ platform dispenser from Shanghai Kinbio

(3) High Emergence of Multidrug-Resistant Sequence Type 131 Subclade C2 among Extended-Spectrum β-Lactamase (ESBL)-Producing Escherichia coli Isolated from the University Hospital Bratislava, SlovakiaAntibiotics (Basel)July 20, 2023Ján Koreň, Michal Andrezál, Elham Ozaee, Hana Drahovská, Martin Wawruch, Adriána Liptáková, Tibor MaliarWaltham, MA, USA); and 2 μL of template DNA, with a total volume of 25 μL. DNA was amplified using the Biometra TAdvanced (Analytik Jena; Jena, Germany) with a thermal protocol consisting of predenaturation at 98 °C for 2

(4) Lost and Found: The Family of NF-κB Inhibitors Is Larger than Assumed in Salmonid FishInternational Journal of Molecular SciencesJune 16, 2023Doret R. van Muilekom, Bertrand Collet, Henrike Rebl, Kristina Zlatina, Fabio Sarais, Tom Goldammer, Alexander ReblBremen, Germany) and oligonucleotide primers linked with specific restriction sites (Table 4). The Biometra TAdvanced cycler (Analytik Jena, Jena, Germany) was used to amplify the gene fragments according to the following

(5) Morphological and Molecular Identification of Physaloptera alata (Nematoda: Spirurida) in a Booted Eagle (Aquila pennata) from PortugalAnimals (Basel)May 17, 2023Zaida Rentería-Solís, David W. Ramilo, Ronald Schmäschke, Sandra Gawlowska, Jorge Correia, Filipa Lopes, Luís Madeira de Carvalho, Luís Cardoso, Isabel Pereira da Fonsecaobtuse) [21] was used as positive control for both PCR assays. Finally, all PCR reactions were run in a Biometra Tadvanced® Thermal cycler (Analytik Jena, Jena, Germany) and visualized in a 1.5% agarose gel. 2.4. Cloning,

(6) The impact of SGLT2 inhibitors on αKlotho in renal MDCK and HK-2 cellsFront Endocrinol (Lausanne)March 8, 2023Lisa Wolf, Michael Föller, Martina FegerGoScript Reverse Transcription System (Promega, Mannheim, Germany). Polymerase chain reaction (PCR) was conducted on a Biometra TAdvanced thermocycler (Analytik Jena, Jena, Germany) using 2 µL cDNA, 0.25 µM canine forward and reverse primers

(7) ATP binding by an F1Fo ATP synthase ε subunit is pH dependent, suggesting a diversity of ε subunit functional regulation in bacteriaFrontiers in Molecular BiosciencesFebruary 27, 2023Alexander Krah, Timothy Vogelaar, Sam I. de Jong, Jolyon K. Claridge, Peter J. Bond, Duncan G. G. McMillanQ5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs), 10 p.m. of each primer and 10 ng DNA. Amplification was performed in a Biometra TAdvanced PCR machine (Analytik Jena), using the following program: 30 s 98 °C, 30x (5 s 98 °C, 30 s. 72 °C),

(8) Lactiplantibacillus argentoratensis and Candida tropicalis Isolated from the Gastrointestinal Tract of Fish Exhibited Inhibitory Effects against Pathogenic Bacteria of Nile TilapiaVeterinary SciencesFebruary 7, 2023Noppadon Siangpro, Songkran Chuakrut, Wanna Sirimanapong, Somboon Tanasupawat, Wongsakorn Phongsopitanun, Bunyarit Meksiriporn, Jarungwit Boonnorat, Siripun Sarin, Siriwat Kucharoenphaibul, Rumpa JutakanokeCTC A-3′) and 1492R (5′-GGT TAC CTT GTT ACG ACT T-3′) as primers [29]. The 16S rDNA was amplified by Biometra TAdvanced Thermal Cycler (Analytik Jena). Successful amplification of 16S rDNA was validated by visual inspection

(9) Genetic Manipulation of CB1 Cannabinoid Receptors Reveals a Role in Maintaining Proper Skeletal Muscle Morphology and Function in MiceInternational journal of molecular sciencesDecember 9, 2022Singlár Z, Ganbat N, Szentesi P, Osgonsandag N, Szabó L, Telek A, Fodor J, Dienes B, Gönczi M, Csernoch L, et al.isolated from finger biopsies and screened for the presence of the HSA-Cre recombinase cassette by PCR (Biometra Tadvanced Twin 48 G, 230 V, Analytik Jena GmbH, Jena, Germany) using the appropriate primers spanned the C-terminus

(10) Rapid Detection of Attomolar SARS-CoV-2 Nucleic Acids in All-Dielectric Metasurface BiosensorsBiosensors (Basel)November 8, 2022Masanobu Iwanagatemperatures were first set to 60–70 ∘C for 60 min and then down to 25 ∘C for 5 min in a thermal cycler (Biometra TAdvanced 96SG, Analytik Jena, Gettingen, Germany). After the reaction, FL was measured in an automated system

(11) Role of EmaSR in Ethanol Metabolism by Acinetobacter baumanniiInternational Journal of Molecular SciencesOctober 20, 2022Hung-Yu Shu, Yu-Wen Huang, Ping-Yi Tsai, Kun-Sheng Hsieh, Guang-Huey LinUSA), 100 mM of dithiothreitol (DTT), 2.5 mM dNTP, and 1 nM of hexamer. The reaction was conducted in a Biometra TADVANCED Thermal Cycler (Analytik Jena, Jena, Germany). Gene-specific primers used to determine the presence

(12) The Toll-like Receptor 4 Polymorphism Asp299Gly Is Associated with an Increased Risk of Ovarian CancerCellsOctober 5, 2022Katarzyna D. Kania, Daria HarÄ™Å¼a, Jacek R. WilczyÅ„ski, MiÅ‚osz WilczyÅ„ski, Dariusz Jarych, Andrzej Malinowski, Edyta Paradowskacycles each of 30 s at 95 °C, 20 s at 60 °C, and 30 s at 72 °C. The reactions were performed in the Biometra TAdvanced thermal cycler (Analytik Jena Gmbh, Göttingen, Germany) and T100 Thermal Cycler (Bio-Rad, Hercules,

(13) Carotenoids in orange carrots mitigate non-alcoholic fatty liver disease progressionFrontiers in NutritionSeptember 26, 2022Emilio Balbuena, Junrui Cheng, Abdulkerim Eroglusamples, 1 μL random primer, 1 μL dNTP, 5 μL RT buffer, 0.5 μL RNase inhibitor, and 1 μL RTase in a Biometra TAdvanced 96G Thermal Cycler (Analytik Jena, Jena, Germany). The program conditions were 25°C for 10 min, 42°C

(14) Characterization of Colletotrichum Isolates from Strawberry and Other Hosts with Reference to Cross-Inoculation PotentialPlants (Basel)September 11, 2022Gunjan Sharma, Marcel Maymon, Vineet Meshram, Stanley Freeman2022), 0.5 µL each of 10 µM forward and reverse primer and 7.5 µL of sterile water in a thermocycler (Biometra TAdvanced, Analytik-Jena, Jena, Germany). The cycling parameters were: initial denaturation at 95 °C for 4 min,

(15) QTL Mapping for Resistance to Bacterial Wilt Caused by Two Isolates of Ralstonia solanacearum in Chili Pepper (Capsicum annuum L.)Plants (Basel)June 10, 2022Saeyoung Lee, Nidhi Chakma, Sunjeong Joung, Je Min Lee, Jundae LeeCarlsbad, CA, USA), and 10−5 μL of each primer in a final reaction volume adjusted to 20.0 μL, using TDW. Biometra TAdvanced (Analytik Jena AG, Jena, Germany) was used for the PCR reaction. After performing the initial denaturation

(16) Altered Brain Arginine Metabolism and Polyamine System in a P301S Tauopathy Mouse Model: A Time-Course StudyInternational Journal of Molecular SciencesMay 27, 2022Hannah Mein, Yu Jing, Faraz Ahmad, Hu Zhang, Ping Liu(Applied Biosystems, 4387406) was used to convert the RNA to cDNA as per the vendor’s instructions on a Biometra TAdvanced PCR Thermal Cycler (Analytik, Jena, Germany). RT-qPCR (triplets per sample) was performed with cDNA

(17) Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cellsiScienceMay 20, 2022Thomas Bayer, Lara Pfaff, Yannick Branson, Aileen Becker, Shuke Wu, Uwe T. Bornscheuer, Ren Wei(Invitrogen, Darmstadt, Germany). Primer sequences are given in Table S1. PCRs were performed on a Biometra TAdvanced thermal cycler (Analytik Jena, Jena, Germany) employing Q5® polymerase as suggested by the supplier

(18) Ceratocystiopsis quercina sp. nov. Associated with Platypus cylindrus on Declining Quercus suber in PortugalBiology (Basel)May 13, 2022Maria L. Inácio, José Marcelino, Arlindo Lima, Edmundo Sousa, Filomena Nóbregawater (Sigma-Aldrich, St. Louis, MI, USA). Amplification reactions were performed in the thermocycler Biometra TAdvanced (Analytik, Jena, Germany). Amplification of the various loci was performed under the following conditions:

(19) Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in UkraineVirusesApril 28, 2022Nataliia Rudova et al.eluate was run in a 25 μL reaction, with an annealing step at 60 °C, on a conventional PCR machine (Biometra TAdvanced Thermal Cycler, Analytik Jena GmbH). 2.3. Amplification and Sequencing of ORF2 (Cap) and PCV2 Genomes Positive

(20) For the road: calibrated maternal investment in light of extracellular symbiont transmissionProceedings of the Royal Society B: Biological SciencesApril 27, 2022Inès Pons, Miguel Ángel González Porras, Noa Breitenbach, Jürgen Berger, Katharina Hipp, Hassan Salemextraction (electronic supplementary material, table S2). Diagnostic PCR was conducted on an Analytik Jena Biometra TAdvanced Thermal Cycler using a final volume of 20 µl containing 1 µl of DNA template, 0.5 µM of each primer

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Biometra TAdvanced Thermal Cycler Series

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Biometra TAdvanced Thermal Cycler Series

Biometra TAdvanced Thermal Cycler Series from Analytik Jena

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Biometra TAdvanced Thermal Cycler Series from Analytik Jena

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