Wizard SV Gel and PCR Clean-Up System from Promega

Product Specs
ItemWizard SV Gel and PCR Clean-Up System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA9285
Quantity1,000 preps
SourceReaction, Gel
Nucleic Acid TypeDNA Fragment, PCR Product

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Phase separation of FSP1 promotes ferroptosisNatureJuly 13, 2023Toshitaka Nakamura, Clara Hipp, André Santos Dias Mourão, Jan Borggräfe, Maceler Aldrovandi, Bernhard Henkelmann, Jonas Wanninger, Eikan Mishima, Elena Lytton, David Emler, et al.R045A) and resulting PCR products were purified by Wizard SV Gel&PCR Clean-up System (Promega, cat. no. A9285) according to the manufacturer’s protocol. Ligation reactions of PCR products or single guide RNA (sgRNA)

(2) Visualization of Actin Cytoskeleton in Cellular Protrusions in Medaka EmbryosBIO-PROTOCOLJuly 5, 2023Toru Kawanishi, Ann Kathrin Heilig, Atsuko Shimada, Hiroyuki Takeda(LMP) (Promega, catalog number: V2831)Wizard SV gel and PCR clean-up system (Promega, catalog number: A9285)mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific, catalog number: AM1340). 2× NTP/CAP,

(3) easyPACId, a Simple Method for Induced Production, Isolation, Identification, and Testing of Natural Products from ProteobacteriaBIO-PROTOCOLJuly 5, 2023Edna Bode, Daniela Assmann, Petra Happel, Elmar Meyer, Karin Münch, Nicole Rössel, Helge B. Bodekit (Qiagen, catalog number: 27106)Wizard® SV Gel and PCR Clean-Up System (Promega, catalog number: A9285)NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, catalog number: E2621L)Methanol optima

(4) Transcription activator WCC recruits deacetylase HDA3 to control transcription dynamics and bursting in NeurosporaScience AdvancesJune 30, 2023Michael Oehler, Leonie Geisser, Axel C. R. Diernfellner, Michael Brunnerextracted using the Promega PCR Cleaning Kit (Wizard SV Gel and PCR Clean-Up System, Promega, catalog no. A9285) as indicated in the manual. DNA was eluted from the columns with 120 μl (ChIP-samples) or 50 μl (input

(5) Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter GenesMicrobiology SpectrumDecember 21, 2022Chengjin Ye, Luis Martinez-Sobridono. R0547L), PciI (New England Biolabs, catalog no. R0655L), PCR cleanup system (Promega, catalog no. A9285), plasmid maxiprep kit (Omega, catalog no. D6922-04), plasmid miniprep kit (Omega, catalog no. D6942-02),

(6) Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siroBMC MicrobiologyOctober 19, 2022Jan Hubert, Blanka Navratilova, Bruno Sopko, Marta Nesvorna, Thomas W. Phillipsrestriction (Cat. No. R6061, Promega) and cleaned with a Wizard SV gel and PCR Clean-Up system (Cat No. A9285). The concentration of the cleaned product was measured on a P330 Implen NanoPhotometer (Munich, Germany)

(7) A dual-antigen self-amplifying RNA SARS-CoV-2 vaccine induces potent humoral and cellular immune responses and protects against SARS-CoV-2 variants through T cell-mediated immunityMolecular TherapySeptember 7, 2022Sean McCafferty et al.verification. Plasmid digests were purified via silica spin columns (Wizard SV gel and PCR Clean-up System A9285; Promega, Madison, WI, USA). Linearized plasmid DNA was used as a template for the IVT reaction via

(8) Mitotic checkpoint gene expression is tuned by codon usage biasThe EMBO journalAugust 1, 2022Esposito E, Weidemann DE, Rogers JM, Morton CM, Baybay EK, Chen J, Hauf S.(from Streptomyces griseus),Chem Impex,Cat # 00083 Wizard SV Gel and PCR Clean‐Up System,Promega,Cat # A9285 SuperScript IV First Strand Synthesis System,ThermoFisher,Cat # 18091050 HiScribe T7 ARCA mRNA Kit (with tailing

(9) The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activityNature communicationsJanuary 17, 2022Morgan SM, Tanizawa H, Caruso LB, Hulse M, Kossenkov A, Madzo J, Keith K, Tan Y, Boyle S, Lieberman PM, et al.

(10) Homodimerization regulates an endothelial specific signature of the SOX18 transcription factorNucleic Acids ResearchNovember 30, 2018Mehdi Moustaqil et al.

(11) Transcription Factor Forkhead Regulates Expression of Antimicrobial Peptides in the Tobacco Hornworm, Manduca sextaScientific ReportsDecember 1, 2017Xue Zhong, Munmun Chowdhury, Chun-Feng Li, Xiao-Qiang YuPCR products were recovered by agarose gel electrophoresis-Wizard® SV Gel and PCR Clean-Up System (A9285, Promega) and subcloned into T-Easy vectors (A1360, Promega). Recombinant T-vectors were purified using

(12) pELMO, an optimised in-house cloning vectorAMB ExpressDecember 1, 2017Andrea E. Ramos, Marina Muñoz, Darwin A. Moreno-Pérez, Manuel A. Patarroyo

(13) A Novel Small RNA Regulates Tolerance and Virulence in Shigella flexneri by Responding to Acidic Environmental ChangesFrontiers in Cellular and Infection MicrobiologyMarch 8, 2016Ligui Wang et al.kanamycin resistance gene. The resulting PCR product was gel purified using a gel extraction kit (Promega, A9285). The fused PCR fragment was transformed into the wild-type. ΔSsr1 were identified by screening transformants

(14) Co-expression of Dorsal and Rel2 Negatively Regulates Antimicrobial Peptide Expression in the Tobacco Hornworm Manduca sextaScientific ReportsFebruary 5, 2016Xue Zhong, Xiang-Jun Rao, Hui-Yu Yi, Xin-Yu Lin, Xiao-Hong Huang, Xiao-Qiang YuPCR products were recovered by agarose gel electrophoresis-Wizard® SV Gel and PCR Clean-Up System (A9285, Promega), digested by Dpn I, and then transformed into competent Escherichia coli XL1 Blue cells. The

(15) Characterization of a novel Manduca sexta beta-1, 3-glucan recognition protein (βGRP3) with multiple functionsInsect Biochemistry and Molecular BiologySeptember 1, 2014Xiang-Jun Rao, Xue Zhong, Xin-Yu Lin, Xiao-Hong Huang, Xiao-Qiang YuThe PCR product was recovered by agarose gel electrophoresis-Wizard® SV Gel and PCR Clean-Up System (A9285, Promega), subcloned into T-Easy vector (A1360, Promega), and recombinant plasmid was isolated. After

(16) Impact of Technical Sources of Variation on the Hand Microbiome Dynamics of Healthcare WorkersPLoS ONEFebruary 14, 2014Mariana Rosenthal, Allison E. Aiello, Carol Chenoweth, Deborah Goldberg, Elaine Larson, Gregory Gloor, Betsy Foxmanpooled at approximately equal concentrations and purified using a Wizard PCR Clean-Up Kit (Promega; #A9285). DNA Sequencing and Sequence Reads Filtering Sequencing reactions were carried out on three Ion Torrent

(17) Manduca sexta gloverin binds microbial components and is active against bacteria and fungiDevelopmental & Comparative ImmunologyOctober 1, 2012Xiao-Xia Xu, Xue Zhong, Hui-Yu Yi, Xiao-Qiang Yuend. PCR product was recovered by agarose gel electrophoresis-Wizard® SV Gel and PCR Clean-Up System (A9285, Promega), subcloned into T-Easy vector (A1360, Promega), and recombinant plasm id was isolated. After

(18) A Toll-Spätzle pathway in the tobacco hornworm, Manduca sextaInsect Biochemistry and Molecular BiologyJuly 1, 2012Xue Zhong, Xiao-Xia Xu, Hui-Yu Yi, Christopher Lin, Xiao-Qiang YuPCR products were recovered by agarose gel electrophoresis-Wizard® SV Gel and PCR Clean-Up System (A9285, Promega) and subcloned into T-Easy vectors (A1360, Promega). Plasmid DNAs in T-vectors were purified

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(1)

1/14/2020Purification of DNA Restriction Digestion Reactions for CloningDr. Thippeswamy SannasiddappaHighly suggest this kit for purification of restriction reactions or PCR reactions for successful cloning method.
(1)

12/1/2014Purification of PCR products for cloning Lee-Ann HwangI routinely cloning PCR products to various plasmids for expression and conditional knock down experiments. I need high purity PCR product for reliable cloning into my target plasmids. I use Promega... Read Review